Antimicrobial Therapies for HLB and Canker Stephanie Slinski, CRDF Antimicrobial Project Manager Citrus Research and Development Foundation, Inc. 700 Experiment Station Road • Lake Alfred • FL • 33850 863-956-8817 citrusrdf.org Florida Citrus Growers’ Institute April 7, 2015
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Antibiotics are used for prevention of treatment of landscape or specimen plants4/27/2015 www.citrusrdf.org 7
Major Hurdles to Finding an Effective Therapy
•Delivery Foliar delivery for a vascular pathogen?
o Trunk injection
o Penetrants, new chemistries
•Pesticide Registration Most effective therapies have a long regulatory pathway
4/27/2015 www.citrusrdf.org 8
Goals of CLas Chemical Therapy
•Stabilize/reverse decline of chronically infected trees
•Reduce bacterial titer
•Allow for recovery of fruit production while replanting
•Treat new infections early
•Sequential development of better tools
Not viewed as permanent solution
4/27/2015 www.citrusrdf.org 9
CRDF Antimicrobial Therapy Efforts
• Testing materials from industry and researchers Known agricultural bactericides
o Streptomycino Oxytetracycline
Re-purposed bactericides – human and vet med Biopesticides – registered and new Plant Essential Oils EPA 25(b) Exempt List and GRAS-like (FDA) Copper and other metals Libraries of active ingredients - industry
•Discovery – new actives through funded research
4/27/2015 www.citrusrdf.org 10
Evaluation Criteria
• Bactericidal activity
• Dose response
• Formulation for phloem movement
• Application methods – foliar, trunk, roots
4/27/2015 www.citrusrdf.org 11
Stepwise Assay System
Field Use
Liberibacter crescens (laboratory) assay-tests bactericidal activity and dose response
Flush or detached leaf (laboratory) assay-tests activity against CLas, local movement, dose response, phytotoxicity
4/27/2015 www.citrusrdf.org 12
Secondary Evaluation Criteria
• Manufacturing and Scale-Up
• Intellectual Property
• Regulatory Feasibility
• Cost to Grower
• Availability/Time-to-Market
4/27/2015 www.citrusrdf.org 13
Antimicrobial Therapy Prioritization
• Materials are prioritized based on evaluation criteriaand ranked within antimicrobial class using an “Antimicrobial Therapy Candidate Matrix”