ANTIMICROBIAL PROPERTIES OF VARIOUS GREEN TEA PRODUCTS WITH AND WITHOUT THE ADDITION OF SUGAR T3 Members: Samantha Acevedo, Conan Chen, Ethan France, Brian Goldspiel, Anna Kim, Amanda Li, Florence Ma, Sana Siddiqui, Stephanie Tarlowe, Anthony Yakely, Leanna Zhan Advisor: Mrs. Rachel Sandler Assistant: Gillian Bradley
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ANTIMICROBIAL PROPERTIES OF VARIOUS GREEN TEA PRODUCTS WITH AND WITHOUT THE ADDITION OF SUGAR T3 Members: Samantha Acevedo, Conan Chen, Ethan France, Brian.
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ANTIMICROBIAL PROPERTIES OF VARIOUS GREEN TEA PRODUCTS WITH AND
WITHOUT THE ADDITION OF SUGAR
T3 Members:Samantha Acevedo, Conan Chen, Ethan France, Brian
Goldspiel, Anna Kim, Amanda Li, Florence Ma, Sana Siddiqui, Stephanie Tarlowe, Anthony Yakely, Leanna Zhan
• No observable zone of inhibition around disks of any teas
Paper Disk Diffusion: Possible Errors
• Contaminated plates– Open disks exposed
to fungus in lab– Lapses in aseptic
technique– Excess liquid on
disks
Paper Disk Diffusion:Zone of Growth
• Lipton ® tea with sugar– 0.95 cm diameter
• No growth on control
• Sugar may have boosted
• Inconclusive Gram stain
• More research needed
In Vitro:Results
• Control had more bacteria before than after• All results compared to control
– Less bacteria not necessarily significant
• Sugar appeared to have no effect
Control (Water) Matcha with Sugar
In Vitro:Best Results
Loose Leaves Scope ®
In Vitro:Results Discussion
• More subjective and qualitative– Visual observation
• Little clear evidence of antimicrobial benefits against S. mutans
• Loose tea leaves had strongest effect
In Vitro:Alternate Method for Scope ®
• Known antibacterial agent• Loop immersed in Scope ® for 30
seconds• Simulated difference between rinsing
with mouthwash and sipping a beverage
• May have attributed to differing results
In Vitro:Errors
• Preparation method• Small sample size• Discrepancies
Future Research
• Different preparation methods• Potency of green tea on other
microbes• Chemical compositions of teas
– Loose leaf tea (in vitro)
• Effects of sugar• Modeled after realistic experience• Different concentrations
Conclusions
• Scope ® is most effective• Loose sencha leaves (in vitro)• Sugar inconclusive
– Possibly detrimental in one case
Acknowledgements
• John and Laura Overdeck• The Crimmins Family Charitable Foundation• NJGSS Alumni and Parents (1984-2011)• Bristol-Myers Squibb• Roche• Kinder Morgan• Bain Capital• GlaxoSmithKline• Novartis• Bayer HealthCare• Dr. Miyamoto, Dr. Surace, Dr. Quinn, Myrna