ANNUAL REPORT 2014Current research focuses on the cellular and molecular mechanisms underlying the pathogenesis to of Cerebral Cavernous Malformations (CCM), a major cerebrovascular

Department of Clinical

and Biological Sciences

University of Turin

ANNUAL REPORT

2014

DIRECTOR: Prof. Mario DE MARCHI

INDEX

ADAPTED TRAINING AND PERFORMANCE TEAMS (A.T.P.T.) 1

Dr. Massimiliano GOLLIN, Assistant Professor

APPLIED BIOLOGY 3

Prof. Saverio F. RETTA, Associate Professor

BIOSTATISTICS 5

Prof. Giuseppe MIGLIARETTI, Associate Professor

CELL BIOLOGY 10

Prof. Salvatore BOZZARO, Full Professor

CELLULAR NEUROBIOLOGY 11

Prof. Isabelle PERROTEAU, Full Professor

CLINICAL PATHOLOGY AND NEPHROLOGY

Prof. Dario ROCCATELLO, Full Professor

ENDOCRINOLOGY, DIABETOLGY AND METABOLISM 18

Dott. Roberta GIORDANO, Assistant Professor

EXPERIMENTAL MEDICINE AND CLINICAL PATHOLOGY 20

Prof. Giuseppina BARRERA, Associate Professor

FARMACOLOGY 22

Dr. Giovanni N. BERTA, Assistant Professor

GENERAL PATHOLOGY 24

Dr. Riccardo AUTELLI, Assistant Professor

GENERAL PATHOLOGY - CANCER CACHEXIA 26

Prof. Gabriella BONELLI, Full professor

GENERAL PATHOLOGY - LABORATORY FOR THE STUDY OF NUTRITION

AND METABOLIC DISORDERS 28

Prof. Manuela ARAGNO, Associate Professor

GENERAL PATHOLOGY AND PATHOPHYSIOLOGY 30

Prof. Giuseppe POLI, Full Professor

HEMATOLOGY AND STEM CELL TRANSPLANTATION LABORATORY 33

Prof. Daniela CILLONI, Associate Professor

HUMAN ANATOMY 36

Prof. Maria G. ROBECCHI, Full Professor

HUMAN AND INDUSTRIAL TOXICOLOGY 39

Dr. Giovanni MAINA, Assistant Professor

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MEDICAL GENETICS - HUMAN GENETICS GROUP 41

Prof. Claudia GIACHINO, Associate Professor

HUMAN PHYSIOLOGY - CARDIOVASCULAR PHYSIOLOGY 43

Prof. Pasquale PAGLIARO, Associate Professor

INTERNAL MEDICINE 3 - METABOLIC DISEASE UNIT 46

Prof. Angelo GUERRASIO, Associate Professor

INTERNAL MEDICINE I 48

Prof. Massimo TERZOLO, Associate Professor

IRON METABOLISM 52

Prof. Giuseppe SAGLIO, Full Professor

LIVER & GUT PATHOPHYSIOLOGY 55

Prof. Maurizio PAROLA, Full Professor

MOLECULAR ONCO-HEMATOLOGY 57

Dr. Alessandro MOROTTI, Assistant Professor

MOLECULAR BIOLOGY 59

Prof. Michele DE BORTOLI, Full Professor

MOLECULAR VIROLOGY AND ANTIVIRAL RESEARCH 62

Prof. David LEMBO, Associate Professor

NEPHROLOGY AND CLINICAL NUTRITION 65

Prof. Paolo AVAGNINA, Associate Professor

NEUROLOGY 69

Prof. Luca DURELLI, Full Professor

OBSTRUCTIVE LUNG DISEASE 71

Prof. Fabio L.M. RICCIARDOLO, Assistant Professor

PEDIATRICS 74

Prof. Antonio G. PIGA, Associate Professor

PHARMACOLOGY AND CLINICAL PHARMACOLOGY SERVICE 77

Prof. Silvia RACCA, Associate Professor

PSYCHIATRY 79

Dr. Rocco Luigi PICCI, Assistant Professor

ROLE OF IRON METABOLISM PROTEINS IN HEMATOLOGIC MALIGNANCIES 81

Dr.Marco DE GOBBI, Assistant Professor

Research Team

www.unito.it/dscb

ADAPTED TRAINING AND PERFORMANCE TEAMS (A.T.P.T.)

Dr. Massimiliano

GOLLIN Assistant Professor

Dr. Luca

BERATTO PhD Student

Dr. Marco

ALESSANDRIA PhD Student

Dr. Luca

BASEGGIO Technical Assistant

Test

Dr. Alessandro

BINELLI Technical Assistant

Test

Dr. Ruben

ALLOIS Technical Assistant

Test

Dr. Federico

ABATE DAGA Technical Assistant

Test

Current Research Projects

Current research projects are focused in these main directions:

To investigate gate differences between asymmetric and symmetric sport disciplines to

prevent muscle damages.

To investigate whether adapted physical activity can promote brain plasticity in Parkinson's

desease.

To investigate the influence of a short circuit training (program) performed with elastic

bands in wheelchair basketball athletes.

To investigate the influence of multifactorial neuromuscular stimulation through adapted

physical activity in multiple sclerosis.

To investigate whether masticatory cycle analysis can be a prevention screening in multiple

sclerosis.

To investigate the effect of core stability and proprioceptive unbalance exercises in Parkinson's

desease.

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Research products

Recent Publications (2013/2014)

Books

Gollin M., Metodologia della preparazione fisica, Elika Edizioni, Cesena 2014.

Book chapters

Gollin M., Autore dei Capitoli II e VII del libro Preparazione allo sport: manuale per la

preparazione fisica di base. Ed. SDS, Roma 2014: 59-80; 135-154 – ISBN: 9788897337102.

International journal

Mental health & physical activity

Roppolo M, Mulasso A, Gollin M, Bertolotto A, Ciairano S The role of fatigue in the

associations between exercise and psychological health in multiple sclerosis: direct and indirect

effects, Mental Health & Physical Activity, 2013 (accepted) 6: 87-94 – ISSN 1755-2966.

Medicina dello sport

Gollin M, Beratto L, Strength exercise with and without self-adapted mouthguard: an acute

study. Int J Sports Med. 2014 Sep (in press).

National journal

Sport school journal

Gollin M, Beratto L,, Allois R., Serravite N, Mazzei P, L’allenamento della forza con

sovraccarichi e il wheelchair tennis: studio longitudinale, SDS - Rivista di Cultura Sportiva, 2014,

102: 61-66.

Gollin M,, Gualtieri A, Baseggio L, L’influenza della stimolazione sonora sulla prestazione

di forza, SDS - Rivista di Cultura Sportiva, 2014, 101: 47-51.

Gollin M., Beratto Luca, Abate Daga F., Effetti ritardati dell’attività di elongazione

muscolo-tendinea di breve durata, SDS - Rivista di Cultura Sportiva, 2013, (98): 65-70 - ISSN:

1125-1891.

Gollin M. , Piancino MG, Rainoldi A, Bracco P, L’allenamento della forza dinamica e

l’utilizzo del bite, SDS - Rivista di Cultura Sportiva, 2013, (96): 55-62 - ISSN: 1125-1891.

Strength & cinditioning

Gollin m., Guerra A., Le variazioni circadiane dell’efficienza fisica nella lotta greco romana

(seconda parte), strength conditioning (Italy), 2014, 8: 40-44, issn: 2281-3772.

Gollin M., Guerra A., le variazioni circadiane dell’efficienza fisica nella lotta greco romana

(prima parte), strength conditioning (Italy), 2013, 7: 39-41, issn: 2281-3772.

Gollin M., Beratto L., baseggio luca, luciano a., forza, estensibilità muscolo-tendinea e

stretching statico, strength conditioning (Italy), 2013, 4: 37-41, issn: 2281-3772.

Sport science

Gollin M., Beratto Luca PhDc, Mazza C, Roppolo M PhDc, Mulasso A PhDc, Il ruolo

dell’esercizio fisico adattato in soggetti con SM: metodo pilates vs stretching statico, Scienza e

Sport (Italy), 2014, 24: 74-79 SSN: 2039-0726.

Gollin M., Allois R., Binelli A., Il Kinesiotaping e la prestazione di forza, Scienza e Sport

(Italy), 2014, 22: 66-71, ISSN: 2039-0726.

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APPLIED BIOLOGY

Prof. Saverio F.

RETTA Associate Professor

Dr. Luca

GOITRE Postdoctoral

Fellowship

Dr. Eliana

TRAPANI PhD Student


Current research focuses on the cellular and molecular mechanisms underlying the pathogenesis to

of Cerebral Cavernous Malformations (CCM), a major cerebrovascular disease of proven genetic

origin affecting 0.3-0.5% of the general population. It is characterized by abnormally enlarged and

leaky capillaries that predispose to seizures, focal neurological deficits and intracerebral

hemorrhage. However, CCM lesions occur in a range of different phenotypes, including wide

differences in lesion number, size and susceptibility to intracerebral hemorrhage, suggesting that

multiple factors can contribute to CCM disease pathogenesis. Indeed, only approximately 30% of

people with CCM lesions eventually develop symptoms, which usually show an age-dependent

onset and progression.

Pharmacological treatment is not yet available; however, the characterization of molecular

mechanisms underlying CCM disease is providing fundamental insights into the development of

novel therapeutic approaches.

Major current research projects on this topic are the following:

Characterization of molecular and cellular mechanisms underlying the pathogenesis of

Cerebral Cavernous Malformations (CCM);

Identification of risk factors of diagnostic and prognostic value associated with onset,

progression and severity of CCM disease;

Multidisciplinary and integrative research approaches involving multiple clinical and basic

research groups of the CCM_Italia network (http://www.ccmitalia.unito.it/) for the

development of novel, safe and effective pharmacological therapies for CCM disease

prevention and treatment.

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http://www.ccmitalia.unito.it/


Gibson C., Zhu W., Davis C.T., Bowman-Kirigin J.A., Chan A.C., Ling J., Walker A.E.,

Goitre L., Delle Monache S., Retta S.F., Shiu Y-T E., Grossmann A.H., Thomas K.R., Donato

A.J., Lesniewski L.A., Whitehead K.J., Li D.J. Strategy for Identifying Repurposed Drugs for

the Treatment of Cerebral Cavernous Malformation. Circulation 2014, in press.

Fontanella MM, Panciani PP, Spena G, Roca E, Migliorati K, Ambrosi C, Sturiale CL, Retta

SF (2014) Professional athletes and cerebral cavernomas: an obstacle to overcome. Sports

Med Phys Fitness. 2014 Mar 18. [Epub ahead of print].

Goitre L, De Luca E, Braggion S, Trapani E, Guglielmotto M, Biasi F, Forni M, Moglia A,

Trabalzini L, Retta SF. (2014) KRIT1 loss of function causes a ROS-dependent upregulation

of c-Jun. Free Radic Biol Med., 68C:134-147.

Goitre L, Trapani E, Trabalzini L, Retta SF (2014) The ras superfamily of small GTPases: the

unlocked secrets. Methods Mol Biol. 2014;1120:1-18.

Ferro E, Goitre L, Baldini E, Retta SF, Trabalzini L. (2014) Ras GTPases are both regulators

and effectors of redox agents. Methods Mol Biol. 2014;1120:55-74.

Goitre L, Retta SF (2014) Combined pulldown and time-lapse microscopy studies for

determining the role of Rap1 in the crosstalk between integrins and cadherins. Methods Mol

Biol. 2014;1120:177-95.

Goitre L, Cutano V, Retta SF. (2014) Fluorescence microscopy study of Rap1 subcellular

localization. Methods Mol Biol. 2014;1120:197-205.

Maddaluno L, Rudini N, Cuttano R, Bravi L, Giampietro C, Corada M, Ferrarini L, Orsenigo

F, Papa E, Boulday G, Tournier-Lasserve E, Chapon F, Richichi C, Retta SF, Lampugnani

MG, Dejana E. (2013) EndMT contributes to the onset and progression of cerebral cavernous

malformations. Nature, 27;498(7455):492-6.

Di Gregorio E, Bianchi FT, Schiavi A, Chiotto AM, Rolando M, Verdun di Cantogno L,

Grosso E, Cavalieri S, Calcia A, Lacerenza D, Zuffardi O, Retta SF, Stevanin G, Marelli C,

Durr A, Forlani S, Chelly J, Montarolo F, Tempia F, Beggs HE, Reed R, Squadrone S, Abete

MC, Brussino A, Ventura N, Di Cunto F, Brusco A. (2013) A de novo X;8 translocation

creates a PTK2-THOC2 gene fusion with THOC2 expression knockdown in a patient with

psychomotor retardation and congenital cerebellar hypoplasia. J Med Genet, 50(8):543-51.

Orso F, Balzac F, Marino M, Lembo A, Retta SF, Taverna D. (2013) miR-21 coordinates

tumor growth and modulates KRIT1 levels. Biochem Biophys Res Commun, 16;438(1):90-6.

Bacigaluppi S, Retta S.F., Pileggi S, Fontanella M, Goitre L, Tassi L, La Camera A, Citterio

A, Patrosso MC, Tredici G, Penco S. (2013) Genetic and cellular basis of Cerebral Cavernous

Malformations: implications for clinical management. Clinical Genetics, 83(1):7-14.

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BIOSTATISTICS

Prof. Giuseppe

MIGLIARETTI Associate Professor

Dr Paola

BERCHIALLA Assistant Professor


1. Clinical Epidemiology

Efficacy of Growth Hormone therapy (Regional GH Register of Piedmont)

Principal aims:

1. Monitoring the patients treated with GH therapy in Piedmont in the years

2. Evaluation of the efficacy of the treatment using estimated growth curves based on

Bayesian approach

Telemedicine in head injuries (TEMPORE project)

Principal aim: evaluating influence of pain management and relief on the prognosis of patients both

in term of functional recovery and quality of life.

Lung Cancer in young adults: an epidemiological prospective multicenter study

Principal aim: to create a multi-institutional network involving national and international Leader

Institutions in the field of lung cancer research, facing, for the first time, a unique pts

population in order to develop an interactive communication specifically dedicated to young lung

cancer pts.

Epidemiology of Autism in Piedmont

Principal aims:

1. Incidence of Autism in Piedmont in the period 2002-2014 will be estimated and

investiagted using a specific analysis of trend;

2. Using a Bayesian approach, a spatial analysis will be performed to describe the risk of

disease associated to geographical variation.

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2. Methodological research interest, motivated by biomedical research questions

(i) Graphical models for exploring probabilistic relationships among variables and conditional

independencies in the associated probability models with application in a wide range of fields:

epidemiology of foreign body injuries in children, quantitative risk assessment of adverse

cardiovascular events, association of biochemical and clinical factors on fetal growth outcome,

causation and causal inference;

(ii) Hierarchical Bayesian models for integrating data from multiple data sources and Bayesian

Model Averaging, as approach to inference in presence of different competing models;

(iii) Bayesian Design for Phase I/II Clinical Trials

3. Medical education research and evaluation

The research group is actually involved in the evaluation of research and teaching activities of the

DSCB and the San Luigi University hospital. Within this context, multi-criteria decision methods

have been assessed for evaluating medical care, research and teaching activities carried out by

operational units and automation of a computer application has been developed.

4. Statistical applications

Collaborative support and assistance is provided in all areas of biostatistical expertise, including

designing studies with appropriate sample size and power and statistical analysis of both clinical

and biological data.

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Baldi I, Pagano E, Berchialla P, Desideri, Ferrando, Merletti F, Gregori D. Modeling health

care costs in simultaneouss presence of asymmetry, heteroscedasticity and correlation.

Journal of Applied Statitsics 2013; 40(2): 298-310.

Berchialla P, Gangemi EN, Foltran F, Haxhiaj A, Buja A, Lazzarato F, Stella M, Gregori D.

Predicting Severity of Pathological Scarring Due to Burn Injuries: a Clinical Decision Making

Tool Using Bayesian Networks. International Wound Journal 2014 June; 11(3):246-252.

Berchialla P, Scaioli G, Passi S, Gianino MM. Adverse events in hospitalized pediatric

patients: a systematic review and a meta-regression analysis. Journal of Evaluation in

Clinical Practice 2014 20(5): 551-8.

Berchialla P., Scarinzi, C., Snidero, S., Gregori, D. Comparing Models for Quantitative Risk

Assessment: an Application to the European Registry of Foreign Body Injuries in Children.

Statistical Methods in Medical Research 2013 Feb 19 [Epub ahead of print].

Bistolfi A, Lee GC, Deledda D, Rosso F, Berchialla P, Crova M, Massazza G. NexGen® LPS

mobile bearing total knee arthroplasty: 10 year results. Knee Surg Sports Traumatol Arthrosc

2014 22(8): -1786-92.

Bistolfi A, Massazza, Crova, Lee, Deledda, Berchialla P, Crova. Long Term, Prospective

Comparison of Fixed Bearing versus Mobile Bearing TKA. Journal of Bone and Joint

Surgery 2013 Jun 19; 95(12): e83.

Borrelli I, Casalis Cavalchini GC, Del Peschio S, Micheletti M, Venesio T, Sarotto I,

Allavena A, Delsedine L, Barberis MA, Mandrile G, Berchialla P, Ogliara P, Bracco C, Pasini

B. A founder MLH1 mutation in Lynch Syndrome families from Piedmont, Italy, is

associated with an increased risk of pancreatic tumours and diverse immunohistochemical

patterns. Familial Cancer 2014 13(3): 401-13.

Charrier L, Berchialla P, Galeano D, Spizzichino L, Borraccino A, Lemma P, Dalmasso P,

Cavallo F. Smoking Habits among Italian Adolescents: What Has Changed in the Last

Decade? BioMed Research International 2014.

Ciccone MM, Sharma RK, Scicchitano P, Cortese F, Salerno C, Berchialla P, Frasso G,

Sassara M, Carbone M, Palmiero P, Maiello M, Voelker DJ, Reddy HK, Federico F.

Dolichocatotids: Echo-Color Doppler Evaluation and Clinical Role. Atherosclerosis and

Thrombosis 2014 Jan 23;21(1):56-63.

Gonella S, Berchialla P, Bruno B, Di Giulio P. Are orange lollies effective in preventing

nausea and vomiting related to Dymethyl Sulfoxide? A multicenter randomized trial.

Supportive Care Cancer 2014 22(9):2417-24.

Gregori D, Ballali S, Vogele, C, Galasso F, Widhalm K, Berchialla P, Baldi I. What is the

Value Given by Consumers to Nutritional Label Information? Results from a Large

Investigation in Europe. Journal of the American College of Nutrition 2014.

Gregori D, Ballali S, Vecchio MG, Sciré AS, Foltran F, Berchialla P. Randomized Controlled

Trials Evaluating Effect of Television Advertising on Food Intake in Children: Why Such a

Sensitive Topic is Lacking Top-Level Evidence? Ecology of Food and Nutrition 2014 53(5):

562-577.

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Lazzeri G, Giacchi MV, Dalmasso P, Vieno A, Nardone P, Lamberti A, Spinelli A, Cavallo F;

HBSC 2010 Group. The methodology of the Italian HBSC 2010 study (Health Behaviour in

School-aged Children). Annali di Igiene 2013 May-Jun; 25(3).

Mandrile G, van Woerden CS, Berchialla P, Beck BB, Acquaviva Bourdain C, Hulton SA,

Rumsby G. Data from a large European study indicate that the outcome of primary

hyperoxaluria type 1 correlates with the AGXT mutation type. Kidney Int. 2014 Jul 2.

Migliaretti G, Berchialla P. Observational approaches in the study of the effects of Total

Suspendd Particulates (TSP) exposure. International Journal of Environmental Health

Research 2013; 23(5): 392-9.

Migliaretti G, Berchialla P, Dalmasso P, Cavallo F, Chiò A. Amyotrophic lateral sclerosis in

Piedmont (Italy): a Bayesian spatial analysis of the incident cases. Amyotroph Lateral Scler

Frontotemporal Degener. 2013 Jan;14(1):58-65.

Migliaretti G, Ciaramitano P, Berchialla P, Scarinzi C, Andrini R, Orlando A, Faccani G.

Teleconsulting for minor head injury: the Piedmont experience. Journal of telemedicine and

telecare. 2013 Jan 19(1):33-5.

Navone ND, Perga S, Martire S, Berchialla P, Malucchi M, Bertolotto A. Monocytes and

CD4+ T cells contribution to the under-expression of NR4A2 and TNFAIP3 genes in patients

with multiple sclerosis. J Neuroimmunol 2014 272(1-2):99.102 pii: S0165-5728(14)00142-8.

Patrono D, Paraluppi G, Perino M, Palisi M, Migliaretti G, Berchialla P, Romagnoli R,

Salizzoni M. Posthepatectomy liver failure after simultaneous versus staged resection of

colorectal cancer and synchronous hepatic metastases. Giornale di Chirurgia 2014 Mar-Apr;

35(3-4): 86-93.

Pineiro ALM, Ballali S, Berchialla P, Chiffi D, del Socorro del Rio Angulo M, Giunta F,

Stanchi R, Gregori D. Feeding Children in Rural Area: Proper Nutrition and Psycho-Social

Development. The Open Obesity Journal, 2013, 5, (Suppl 1:M2) 14-21.

Salerno C., Berchialla P., Palin L., Panella M. [Cancer survival in a local health district in

Piemonte (Italy): follow up to 2007]. Igiene e Sanità Pubblica 2013 Jan-Feb; 69(1):39-46.

Salerno C, Berchialla P, Palin LA, Barasolo E, Vanhaecht K, Panella M. Geographical and

epidemiological analysis of oncological mortality in Municipality of North-Western Italy

Vercelli years 2000-2009 [Italian]. Annali di Igiene, 2014, 26(2): 157-66.

Sandri M, Berchialla P, Baldi I, Gregori D, De Blasi R. Dynamic Bayesian Networks to

predict sequences of organ failures in patients admitted to ICU. Journal of Biomedical

Informatics, 2014 48:106-13.

Sicuro J, Bondonio P, Charrier L, Berchialla P, Cavallo F, Porta M, Trento M, Cost analysis

of group versus usual care in patients with type 2 diabetes in the ROMEO clinical trial.

Nutrition, Metabolism and Cardiovascular Diseases 2013 Feb; 23(2):e13-4 (Letter to the

editor) dx.

Sicuro J, Charrier L, Berchialla P, Cavallo F, Merlo S, Mazzeo A, Porta M, Trento M. Self

Management Education by Group Care Reduces Cardiovascular Risk in Patients with Type 2

Diabetes. Analysis of the ROMEO Clinical Trial. Diabetes Care 2014, 37(9):e192-3.

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Terzolo M, Allasino B, Pia A, Peraga G, Daffara F, Laino F, Ardito A, Termine A, Paccotti P,

Berchialla P, Migliaretti G, Reimondo G. Surgical Remission of Cushing’s syndrome reduces

cardiovascular risk. Eur J Endocrinology 2014 171(1):127-36.

Vecchio MG, Berchialla P, Didier N, Cayul O, Valdenegro D, Ghidina M, Gregori D. The

IBAI Instrument to Assess Brand Awareness Toward Food in Children: the Chile Adaptation.

The Open Obesity Journal, 2013, 5, (Suppl 1:M4) 30-35.

Vecchio MG, Ghidina M, Gulati A, Berchialla P, Paramesh EC, Gregori D. Measuring Brand

Awareness as a Component of Eating Habits in Indian Children: The Development of the

IBAI Questionnaire. Indian J Pediatr 2014.

Vernone A, Berchialla P, Pescarmona G. Human Protein Cluster Analysis using Amino acids

Frequencies. PLoS One. 2013 Apr 4; 8(4): e60220.

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CELL BIOLOGY

Prof. Salvatore

BOZZARO Full Professor

Dr. Barbara

PERGOLIZZI Assistant Professor

Dr. Barbara

PERACINO ResearchTechnician

Dr.Simona

BURACCO PhD student


Investigating role and mechanism of action of the Nramp family of iron transporters in the model

organism Dictyostelium

Identifying genes involved in iron homeostasis

Investigating gene networks regulating phagocytosis and host-pathogen interactions

investigating the role of a master ubiquitin ligase in chemotaxis and development

Lab homepage:

http://www.personalweb.unito.it/salvatore.bozzaro/

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http://www.personalweb.unito.it/salvatore.bozzaro/

CELLULAR NEUROBIOLOGY

Prof. Isabelle

PERROTEAU Full Professor

Dr. Giovanna

GAMBAROTTA Assistant Professor

Dr. Giulia

RONCHI Postdoctoral

Fellowship

Dr. Benedetta E.

FORNASARI PhD Student


The current research projects are focused on:

the study of gene expression regulation in the peripheral nerve following nerve injury - focusing

the attention on genes involved in Schwann cell survival, migration and remyelination – with the

final aim of promoting nerve regeneration;

the study of the signal transduction pathways activated downstream different isoforms of ErbB4

and neuregulin1, genes known to confer susceptibility to Schizophrenia.

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Gambarotta G, Ronchi G, Geuna S, Perroteau I. Neuregulin 1 isoforms could be an effective

therapeutic candidate to promote peripheral nerve regeneration. Neural Regen Res. 2014

9:1183-5.

Pascal D, Giovannelli A, Gnavi S, Hoyng SA, de Winter F, Morano M, Fregnan F,

Dell'Albani P, Zaccheo D, Perroteau I, Pellitteri R, Gambarotta G. Characterization of glial

cell models and in vitro manipulation of the neuregulin1/ErbB system. Biomed Res Int.

2014:310215.

Jager SB, Ronchi G, Vaegter CB, Geuna S. The mouse median nerve experimental model in

regenerative research. Biomed Res Int. 2014:701682.

Marvaldi L, Thongrong S, Kozłowska A, Irschick R, Pritz CO, Bäumer B, Ronchi G, Geuna

S, Hausott B, Klimaschewski L. Enhanced axon outgrowth and improved long-distance axon

regeneration in sprouty2 deficient mice. Dev Neurobiol. 2014.

Gambarotta G, Ronchi G, Friard O, Galletta P, Perroteau I, Geuna S. Identification and

validation of suitable housekeeping genes for normalizing quantitative real-time PCR assays

in injured peripheral nerves. PLoS One. 2014 9:e105601.

Gnavi S, di Blasio L, Tonda-Turo C, Mancardi A, Primo L, Ciardelli G, Gambarotta G,

Geuna S, Perroteau I. Gelatin-based hydrogel for vascular endothelial growth factor release in

peripheral nerve tissue engineering. J Tissue Eng Regen Med. 2014.

Tonda-Turo C, Gnavi S, Ruini F, Gambarotta G, Gioffredi E, Chiono V, Perroteau I, Ciardelli

G. Development and characterization of novel agar and gelatin injectable hydrogel as filler for

peripheral nerve guidance channels. J Tissue Eng Regen Med. 2014.

Fregnan F, Gnavi S, Macrì L, Perroteau I, Gambarotta G.The four isoforms of the tyrosine

kinase receptor ErbB4 provide neural progenitor cells with an adhesion preference for the

transmembrane type III isoform of the ligand neuregulin 1. Neuroreport. 2014 25:233-41.

Faroni A, Castelnovo LF, Procacci P, Caffino L, Fumagalli F, Melfi S, Gambarotta G, Bettler

B, Wrabetz L, Magnaghi V.Deletion of GABA-B receptor in Schwann cells regulates remak

bundles and small nociceptive C-fibers. Glia. 2014 62:548-565.

Borrione P, Grasso L, Chierto E, Geuna S, Racca S, Abbadessa G, Ronchi G, Faiola F, Di

Gianfrancesco A, Pigozzi F. Experimental model for the study of the effects of platelet-rich

plasma on the early phases of muscle healing. Blood Transfus. 2014 Suppl 1:s221-8.

Gambarotta G, Fregnan F, Gnavi S, Perroteau I. Neuregulin 1 role in Schwann cell regulation

and potential applications to promote peripheral nerve regeneration. Int Rev Neurobiol.

2013;108:223-56.

Haastert-Talini K, Geuna S, Dahlin LB, Meyer C, Stenberg L, Freier T, Heimann C, Barwig

C, Pinto LF, Raimondo S, Gambarotta G, Samy SR, Sousa N, Salgado AJ, Ratzka A, Wrobel

S, Grothe C. Chitosan tubes of varying degrees of acetylation for bridging peripheral nerve

defects. Biomaterials. 2013 38:9886-904.

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Geuna S, Perroteau I, Tos P, Battiston B.Peripheral nerve repair is no longer a matter of

surgical reconstruction only. Int Rev Neurobiol. 2013;109:xi-xii.

Geuna S, Perroteau I, Tos P, Battiston B. Preface. tissue engineering of the peripheral nerve:

stem cells and regeneration promoting factors. Int Rev Neurobiol. 2013;108:xiii-xiv.

Geuna S, Gnavi S, Perroteau I, Tos P, Battiston B. Tissue engineering and peripheral nerve

reconstruction: an overview. Int Rev Neurobiol. 2013;108:35-57.

Tos P, Ronchi G, Geuna S, Battiston B. Future perspectives in nerve repair and regeneration.

Int Rev Neurobiol. 2013;109:165-92.

Raimondo S, Ronchi G, Geuna S, Pascal D, Reano S, Filigheddu N, Graziani A. Ghrelin: a

novel neuromuscular recovery promoting factor? Int Rev Neurobiol. 2013;108:207-21.

Moimas S, Novati F, Ronchi G, Zacchigna S, Fregnan F, Zentilin L, Papa G, Giacca M,

Geuna S, Perroteau I, Arnež ZM, Raimondo S. Effect of vascular endothelial growth factor

gene therapy on post-traumatic peripheral nerve regeneration and denervation-related muscle

atrophy. Gene Ther. 2013 20:1014-21.

Tonda-Turo C, Cipriani E, Gnavi S, Chiono V, Mattu C, Gentile P, Perroteau I, Zanetti M,

Ciardelli G. Crosslinked gelatin nanofibres: preparation, characterisation and in vitro studies

using glial-like cells. Mater Sci Eng C Mater Biol Appl. 2013 33:2723-35.

Porporato PE, Filigheddu N, Reano S, Ferrara M, Angelino E, Gnocchi VF, Prodam F,

Ronchi G, Fagoonee S, Fornaro M, Chianale F, Baldanzi G, Surico N, Sinigaglia F, Perroteau

I, Smith RG, Sun Y, Geuna S, Graziani A. Acylated and unacylated ghrelin impair skeletal

muscle atrophy in mice. J Clin Invest. 2013 123:611-22.




atrophy. Gene Ther. 2013 20:1014-21.

Jaminet P, Köhler D, Schäufele M, Rahmanian-Schwarz A, Lotter O, Fornaro M, Ronchi G,

Geuna S, Rosenberger P, Schaller HE. Evaluating the role of Netrin-1 during the early phase

of peripheral nerve regeneration using the mouse median nerve model. Restor Neurol

Neurosci. 2013;31:337-45.

Papalia I, Raimondo S, Ronchi G, Magaudda L, Giacobini-Robecchi MG, Geuna S. Repairing

nerve gaps by vein conduits filled with lipoaspirate-derived entire adipose tissue hinders

nerve regeneration. Ann Anat. 2013 195:225-30.



I, Smith RG, Sun Y, Geuna S, Graziani A.Acylated and unacylated ghrelin impair skeletal

muscle atrophy in mice. J Clin Invest. 2013 123:611-22.

Jaminet P, Köhler D, Rahmanian-Schwarz A, Lotter O, Mager A, Fornaro M, Ronchi G,

Geuna S, Rosenberger P, Schaller HE. Expression patterns and functional evaluation of the

UNC5b receptor during the early phase of peripheral nerve regeneration using the mouse

median nerve model. Microsurgery. 2013 33:216-22.

13

CLINICAL PATHOLOGY AND NEPHROLOGY

Prof. Dario

ROCCATELLO Full Professor

Prof. Elisa

MENEGATTI Associate Professor

Dr. Simone

BALDOVINO Assistant Professor

Dr. Savino

SCIASCIA Assistant Professor

Dr. Debora

DI SIMONE Postdoctoral

Fellowship

Dr. Laura

SOLFIETTI Postdoctoral

Fellowship

Dr. Margherita

MESSINA Postdoctoral

Fellowship

Valentina

ODDONE Biomedical Laboratory

Technician


Diagnostic Research

Development of a miniaturized, automated assay for genetic testing

Anti DSG1/DSG3 and anti BP180/230 antibodies ELISA tests for diagnosis and follow-up of

autoimmune bullous dermatoses

The clinical application of the Global Antiphospholipid Syndrome Score

The clinical value of testing for anti-prothrombin and anti-phosphatidylserine/prothrombin

antiphospholipid antibodies

Validation of commercially available kits to detect anti-phosphatidylserine/prothrombin

antibodies

Analysis of the C4 gene polymorphism in patients with cryoglobulinemic vasculities

Clinical and Therapeutic Research

Evaluation of the long term effects of an original treatment protocol based on the anti- CD20

monoclonal antibody administration in patients with HCV-associated mixed cryoglobulinemia and

in ANCA-associated vasculitis

Long term effects of Rituximab in adult patients with Idiopathic Membranous Nephropathy and

Focal Segmental Glomerular Sclerosis

14

Intensified B lymphocyte depletion without immunosuppressive maintenance treatment as a rescue

therapy in refractory lupus and in IgG4-related tubulointerstitial nephritis.

Use of intravenous immunoglobulin in patients with Systemic Lupus Erythematous and with active

vasculitis with concomitant infection

Biosimilars and Autoimmune Diseases

The clinical impact of new oral anticoagulants in patients with antiphospholipid syndrome patients

and previous poor anticoagulation control with vitamin K antagonists

Epidemiologic examination of data of Piedmont and Aosta Valley inter-regional network of Rare

Diseases

15


Rollino C, Ferro M, Beltrame G, Quattrocchio G, Massara C, Quarello F, Roccatello D. Renal

biopsy in patients over 75: 131 cases. Clin Nephrol. 2014 Aug 27.

Sciascia S, Sanna G, Murru V, Roccatello D, Khamashta MA, Bertolaccini ML. The global

anti-phospholipid syndrome score in primary APS. Rheumatology (Oxford). 2014 Aug 13. pii:

keu307.

Borgio C, Mercandino S, Fantino M, Vallero A, Beltrame G, Canton AM, Sillano M, Pezzana

A, Rollino C, Roccatello. D. Low protein dinner: patients in the kitchen. G Ital Nefrol. 2014

Jul-Aug;31(4). pii: gin/31.4.16. Italian.

Sciascia S, Cuadrado MJ, Sanna G, Murru V, Roccatello D, Khamashta MA, Bertolaccini

ML. Thrombotic Risk Assessment in Systemic Lupus Erythematosus: Validation of the

Global Antiphospholipid Syndrome Score (GAPSS) in a prospective cohort. Arthritis Care

Res (Hoboken). 2014 Jun 25.

Sciascia S, Bertolaccini ML, Roccatello D, Khamashta MA, Sanna G. Autoantibodies

involved in neuropsychiatric manifestations associated with systemic lupus erythematosus: a

systematic review. J Neurol. 2014 Jun 21.

Baldovino S, Menegatti E, Modena V, Maspoli M, Avanzi F, Roccatello D. Piedmont and

Aosta Valley inter-regional network in the context of the Italian National Network for rare

diseases. Blood Transfus. 2014 Apr;12 Suppl 3:s617-20. doi: 10.2450/2014.0055-14s.

Taruscio D, Kodra Y, Ferrari G, Vittozzi L; National Rare Diseases Registry Collaborating

Group. The Italian National Rare Diseases Registry.Blood Transfus. 2014 Apr;12 Suppl

3:s606-13.

Tomaselli V, D'Ambrosio D, Massarelli L, Roccatello D. Effect of ambrisentan on digital

ulcers in systemic sclerosis: a case report. Clin Exp Rheumatol. 2014 Apr 28.

Sciascia S, Cuadrado MJ, Khamashta M, Roccatello D. Renal involvement in

antiphospholipid syndrome. Nat Rev Nephrol. 2014 May;10(5):279-89. Epub 2014 Mar 18.

Capobianco M, Piccoli G, Neve Vigotti F, Scapoli P, Deagostini MC, Albera C, Roccatello D,

Bertolotto A. Interferon beta-related nephropathy and interstitial lung disease: a new

association and a long-term warning. Mult Scler. 2014 Jan 20.

Rollino C, Balbiano R, Caramello P, Roccatello D. The use of tolvaptan in a case of severe

hyponatriemia in SIADH during meningitis. G Ital Nefrol. 2013 Nov-Dec;30(6). pii:

gin/30.6.8. Italian.

Sciascia S, Sanna G, Murru V, Roccatello D, Khamashta MA, Bertolaccini ML. Anti-

prothrombin (aPT) and anti- phosphatidylserine/prothrombin (aPS/PT) antibodies and the risk

of thrombosis in the antiphospholipid syndrome. A systematic review. Thromb Haemost.

2014 Feb;111(2):354-64. Epub 2013 Oct 31.

Camara I, Sciascia S, Simoes J, Pazzola G, Salas V, Karim Y, Roccatello D, Cuadrado MJ.

Treatment with intravenous immunoglobulins in systemic lupus erythematosus: a series of 52

patients from a single centre. Clin Exp Rheumatol. 2014 Jan-Feb;32(1):41-7. Epub 2013 Sep

12.

16

Roccatello D. An innovative approach to the treatment of immune-mediated glomerular

diseases. G Ital Nefrol. 2013 Jan-Feb;30(1). pii: gin/30.1.3. Italian.

Elia F, Pagnozzi F, Laface B, Aprà F, Roccatello D. A victim of the Occam's razor. Intern

Emerg Med. 2013 Dec;8(8):767-8. Epub 2013 May 9.

Mereuta OM, Baldovino S, Errichiello E, Binello GB, Restagno G, Battaglia GG, Mazzucco

G, Roccatello D. Systemic AA amyloidosis as a unique manifestation of a combined mutation

of TNFRSF1A and MEFV genes. Amyloid. 2013 Jun;20(2):122-6. Epub 2013 Mar 6.

Piccoli GB, Daidola G, Attini R, Parisi S, Fassio F, Naretto C, Deagostini MC, Castelluccia

N, Ferraresi M, Roccatello D, Todros T. Kidney biopsy in pregnancy: evidence for

counselling? A systematic narrative review. BJOG. 2013 Mar;120(4):412-27. Epub 2013 Jan

15. Review.

Sciascia S, Sanna G, Murru V, Roccatello D, Khamashta MA, Bertolaccini ML. GAPSS: the

Global Anti-Phospholipid Syndrome Score. Rheumatology (Oxford). 2013 Aug;52(8):1397-

403. Epub 2013 Jan 12.

Menegatti E, Berardi D, Messina M, Ferrante I, Giachino O, Spagnolo B, Restagno G,

Cognolato L, Roccatello D. Lab-on-a-chip: emerging analytical platforms for immune-

mediated diseases. Autoimmun Rev. 2013 Jun;12(8):814-20. Epub 2012 Dec 6. Review.

Bazzan M, Vaccarino A, Stella S, Bertero MT, Carignola R, Montaruli B, Roccatello D,

Shoenfeld Y; Piedmont APS Consortium. Thrombotic recurrences and bleeding events in

APS vascular patients: a review from the literature and a comparison with the APS Piedmont

Cohort. Autoimmun Rev. 2013 Jun;12(8):826-31. Epub 2012 Dec 4. Review.

Roccatello D. Novel diagnostic approaches and cost-benefit balance of treatment of immune-

mediated and rare disease in the era of biologic drugs: lessons from the 15th Turin Congress

on Immune Pathology and Orphan Disease. Autoimmun Rev.2013 Jun;12:793-5. Epub 2012

Dec 4.

Rossi D, Russo A, Manna E, Binello G, Baldovino S, Sciascia S, Roccatello D. The role of

nail-videocapillaroscopy in early diagnosis of scleroderma. Autoimmun Rev. 2013

Jun;12(8):821-5. Epub 2012 Dec 4. Review.

Modena V, Bianchi G, Roccatello D. Cost-effectiveness of biologic treatment for rheumatoid

arthritis in clinical practice: an achievable target? Autoimmun Rev. 2013 Jun;12(8):835-8.

Epub 2012 Dec 3. Review.

Baldovino S, Montin D, Martino S, Sciascia S, Menegatti E, Roccatello D. Common variable

immunodeficiency: crossroads between infections, inflammation and autoimmunity.

Autoimmun Rev. 2013 Jun;12(8):796-801. Epub 2012 Dec 4. Review.

17

Research Team

ENDOCRINOLOGY, DIABETOLGY AND METABOLISM

Dr. Roberta

GIORDANO Assistant Professor

Dr. Elisa

MARINAZZO PhD Student

Federica

FUMAROLA Student

Alessia

RAMPINO Student


The current research projects are focused in four main directions:

to investigate the role of Alprazolam, a benzodiazepine activating GABA receptors, in patients

with either Cushing’s syndrome due to pituitary and adrenal adenoma or subclinical Cushing’s

syndrome

to evaluate metabolic and cardiovascular complications in patients with Cushing's syndrome and

Addison's disease, and in patients with adrenal incidentaloma

to evaluate metabolic and cardiovascular profile in patients with Turner syndrome in comparison

with patient with other form of precocious ovary insufficiency

to evaluate metabolic and cardiovascular profile in patients with adrenal insufficiency under new

glucorticoid replacement therapy with Plenadren in comparison with conventional glucocorticoid

replacement therapy

18

Research products

www.unito.it/dscb


R. Giordano, R. Berardelli, I. Karamouzis, V. D’Angelo, A. Picu, C. Zichi., B. Fussotto, M.

Manzo, G. Mengozzi, E. Ghigo, E. Arvat. Acute administration of Alprazolam, a

benzodiazepine activating GABA receptors, inhibits cortisol secretion in patients with

subclinical but not overt Cushing's syndrome. Pituitary; 2013 Sep;16(3):363-9.

Karamouzis, R. Berardelli, E. Marinazzo, V. D'Angelo, D. Zinna', M. Minetto, C. Zichi,

Fussotto, R. Giordano, E. Ghigo, E. Arvat. The acute effect of Fludrocortisone on basal and

hcrh-stimulated hypothalamic-pituitary-adrenal (HPA) axis in humans. Pituitay; 2013

Sep;16(3):378-85.

R. Scarpa, R. Alaggio, L Norberto, J. Furmaniak, S. Chen, B. Rees Smith, S. Masiero, L.

Morlin, M. Plebani, S. Cervato, F. De Luca, M.C. Salerno, R. Giordano, G. Radetti, L.

Ghizzoni, G. Tonini, F. Farinati, C. Betterle. Tryptophan hydroxylase autoantibodies as

markers of a distinct autoimmune gastrointestinal component of autoimmune polyendocrine

syndrome type 1. J Clin Endocrinol Metab. 2013;98:704-712.

Morelli V, Reimondo G, Giordano R, Della Casa S, Policola C, Palmieri S, Salcuni AS, Dolci

A, Mendola M, Arosio M, Ambrosi B, Scillitani A, Ghigo E, Beck-Peccoz P, Terzolo M,

Chiodini I. Long-term follow-up in adrenal incidentalomas: an italian multicenter study. J

Clin Endocrinol Metab. 2014;99(3):827-834.

Guaraldi F, Parasiliti-Caprino M, Goggi R, Beccuti G, Grottoli S, Arvat E, Ghizzoni L, Ghigo

E, Giordano R, Gori D. Identification of risk conditions for the development of adrenal

disorders: how optimized pubmed search strategies makes the difference. Endocrine. 2014

May 25. [Epub ahead of print]

Karamouzis I, Berardelli R, D'Angelo V, Fussotto B, Zichi C, Giordano R, Settanni F,

Maccario M, Ghigo E, Arvat E. Enhanced oxidative stress and platelet activation in patients

with Cushing's syndrome. Clin Endocrinol (Oxf). 2014 Jun 12. [Epub ahead of print]

19

EXPERIMENTAL MEDICINE AND CLINICAL PATHOLOGY

Prof. Giuseppina

BARRERA Associate Professor

Dr. Stefania

PIZZIMENTI Assistant Professor

Dr. Chiara

ULLIO Postdoctoral

Fellowship

Dr. Eric

CIAMPORCERO PhD Student

Dr. Martina

DAGA PhD Student


Effect of GHS-responsive nanoparticles containing chemotherapeutic agents in chemo-

resistant tumor cells with diverse GSH level.

Effect of GSH-responsive nanoparticles in tumor cell line TUBO vs. derived cancer stem

cells-enriched mammospheres.

The role of Hippo signaling effector YAP in chemoresistance of bladder cancer cells.

Detection of HNE-protein adduct in KRIT1−/− and KRIT1+/+ mouse embryonic fibroblast

(MEF) cell lines were established from KRIT1−/− and KRIT1+/+ E8.5 mouse embryos

20


Eric ciamporcero, Kiersten Marie Miles, Remi Adelaiye, Swathi Ramakrishnan, Li Shen,

Shengyu Ku, Stefania Pizzimenti, Barbara Sennino, Giuseppina Barrera, and Roberto Pili;

Combination strategy targeting VEGF and HGF/c-met in human renal cell carcinoma models.

Molecular Cancer Therapeutics, 2014 in press.

Giuseppina Barrera, Stefania Pizzimenti, Eric Ciamporcero, Martina Daga, Chiara Ullio,

Alessia Arcaro, Gianpaolo Cetrangolo, Carlo Ferretti, Chiara Dianzani, Alessio Lepore, and

Fabrizio Gentile. Role of 4-hydroxynonenal-protein adducts in human diseases; Antioxidant

and Redox Signalling, 2014 in press.

Steimberg N., Mazzoleni G., Ciamporcero E., Ullio C., Daga M., Barrera G., Pizzimenti S. In

Vitro Modeling of Tissue-Specific 3D Microenvironments and Possibile Application to

Pediatric Cancer Research. J Pediatric Oncology 2014; 2(1): 40-76.

Dianzani C, Zara GP, Maina G, Pettazzoni P, Pizzimenti S, Rossi F, Gigliotti CL,

Ciamporcero ES, Daga M, Barrera G. Drug Delivery Nanoparticles in Skin Cancers. Biomed

Res Int. 2014; 2014:895986. Epub 2014 Jul 2. Review.

Pizzimenti S, Ciamporcero E, Pettazzoni P, Osella-Abate S, Novelli M, Toaldo C, Husse M,

Daga M, Minelli R, Bisazza A, Ferruti P, Ranucci E, Grazia Bernengo M, Dianzani C, Biasi

F, Cavalli R, Barrera G. The inclusion complex of 4 hydroxynonenal with a polymeric

derivative of β-cyclodextrin enhances the antitumoral efficacy of the aldehyde in several

tumor cell lines and in a three-dimensional human melanoma model. Free Radic Biol Med.

2013 Dec;65:765-77.

Pizzimenti S, Ciamporcero E, Daga M, Pettazzoni P, Arcaro A, Cetrangolo G, Minelli R,

Dianzani C, Lepore A, Gentile F, Barrera G. Interaction of aldehydes derived from lipid

peroxidation and membrane proteins. Front Physiol. 2013 Sep 4;4:242.

Minelli R, Occhipinti S, Gigliotti CL, Barrera G, Gasco P, Conti L, Chiocchetti A, Zara GP,

Fantozzi R, Giovarelli M, Dianzani U, Dianzani C. Solid lipid nanoparticles of cholesteryl

butyrate inhibit the proliferation of cancer cells in vitro and in vivo models. Br J Pharmacol.

2013 Sep;170(2):233-44.

21

FARMACOLOGY

Dr. Giovanni N.

BERTA Assistant Professor

Dr. Federica

DI SCIPIO Postdoctoral

Fellowship

Dr. Zhiqian

YANG PhD Student

Dr. Maria E.

CARERE PhD Student


•Identification and preclinical validation of novel therapeutic strategies (DNA vaccination included)

efficacious against poor prognosis tumour histotypes (carcinomas).

•Identification and validation of novel nano-molecular vectors for drugs and/or molecules

characterized by unfavorable pharmacokinetics.

•Isolation and characterization of mesenchymal stem cells from tissues of adult mammalians and

their preclinical employment in three-dimensional models.

22


Rizzotto G, Crosetti E, Lucioni M, Bertolin A, Monticone V, Sprio AE, Berta GN, Succo G.

“Oncologic outcomes of supratracheal laryngectomy: Critical analysis.” Head Neck. 2014

May 16.

Di Scipio F, Sprio AE, Folino A, Carere ME, Salamone P, Yang Z, Berrone M, Prat M,

Losano G, Rastaldo R, Berta GN. “Injured cardiomyocytes promote dental pulp mesenchymal

stem cell homing.” Biochim Biophys Acta. 2014 Jul;1840(7):2152-61.

Cristallini C, Cibrario Rocchietti E, Accomasso L, Folino A, Gallina C, Muratori L, Pagliaro

P, Rastaldo R, Raimondo S, Saviozzi S, Sprio AE, Gagliardi M, Barbani N, Giachino C. “The

effect of bioartificial constructs that mimic myocardial structure and biomechanical properties

on stem cell commitment towards cardiac lineage.” Biomaterials. 2014 Jan;35(1):92-104.

Zamperone A, Pietronave S, Merlin S, Colangelo D, Ranaldo G, Medico E, Di Scipio F, Berta

GN, Follenzi A, Prat M. “Isolation and characterization of a spontaneously immortalized

multipotent mesenchymal cell line derived from mouse subcutaneous adipose tissue.” Stem

Cells Dev. 2013 Nov 1;22(21):2873-84.

Ronchi G, Gambarotta G, Di Scipio F, Salamone P, Sprio AE, Cavallo F, Perroteau I, Berta

GN, Geuna S. “ErbB2 receptor over-expression improves post-traumatic peripheral nerve

regeneration in adult mice.” PLoS One. 2013;8(2):e56282.

23

Research Team

www.unito.it/dscb

GENERAL PATHOLOGY – RICCARDO AUTELLI

Dr. Riccardo

AUTELLI Assistant Professor


Lysosomal mechanisms of death in cancer cells.

Hepatocarcinomas (HCC) and HCC-derived cells frequently show resistance to anticancer agents.

The rat hepatoma cells HTC are resistant to many inducers of apoptosis, but are effectively killed

by TNF/cycloheximide treatment. Death is mediated by the lysosomal compartment and relies on

TNF-induced, oxidative stress-mediated lysosomal membrane permeabilization (LMP). Upon

permeabilization, the lysosomal contents are allowed to relocate to the cytoplasm and trigger

necrotic or non-necrotic cell death.

The specific aims of this research are:

- to characterize the mechanisms underlying lysosomal death pathways in HCC and breast cancer

cells, in particular to investigate the contribution of pro- and antiapoptotic Bcl-2 family proteins;

- to investigate whether some of the most widely used anticancer drugs (doxorubicin and others)

rely on LMP for their activity;

- to search for a correlation between development of drug resistance and suppression of LMP;

- to investigate the role of autophagy on increased resistance of tumor cell to anticancer drugs.

Interfering with sphingolipid metabolism to increase drug-induced LMP and death in cancer

cells

Sphingosine (Sph) is a sphingolipid generated by ceramide metabolism. We have shown that

sphingosine is a powerful inducer of LMP and mediates TNF-induced LMP and death in HCC cells.

Cancer cells keep as low as possible the intracellular level of Sph, in order to prevent spontaneous

or drug-induced LMP and death. In many cancers, this is granted by overexpression of sphingosine

kinase 1 (SK1) and, to a lesser extent, of SK2. Such kinases phosphorylate Sph to sphingosine 1

phosphate (S1P), thus converting a proapoptotic factor (Sph) into a molecule (S1P) that enhances

survival of SK-overexpressing cells.

Specific aims of the research are: 1) to investigate the molecular mechanisms of SK1 and 2

disregulation in HCC and breast cancer cell lines; 2) to down-regulate SKs in the above cancer cell

lines and to evaluate whether this affects both S1P/Sph ratio and drug-induced LMP and cell death.

24

Research products

www.unito.it/dscb


Bocca C, Ievolella M, Autelli R, Motta M, Mosso L, Torchio B, Bozzo F, Cannito S,

Paternostro C, Colombatto S, Parola M, Miglietta A. Expression of Cox-2 in human breast

cancer cells as a critical determinant of epithelial-to-mesenchymal transition and invasiveness.

Expert Opin Ther Targets. 2014 18:121-35.

25

GENERAL PATHOLOGY - CANCER CACHEXIA

Prof. Gabriella

BONELLI Full professor

Prof. Paola

COSTELLI Associate Professor

Dr. Fabio

PENNA Assistant Professor

Dr. Daniela

De Stefanis Research Technician

Dr. Domiziana

COSTAMAGNA

Postdoctoral

Fellowship

Dr. Fabrizio

PIN PhD student

Dr Riccardo

BALLARO’ PhD student


Cancer cachexia and muscle wasting: alterations of protein metabolism and myogenic

potential;

Pharmacological, nutritional and training approaches to improve muscle wasting in

experimental cancer cachexia;

Stem cell therapy to restore cancer-induced impaired myogenesis.

26


Penna F, Costamagna D, Pin F, Camperi A, Fanzani A, Chiarpotto EM, Cavallini G, Bonelli

G, Baccino FM, Costelli P. Autophagic degradation contributes to muscle wasting in cancer

cachexia. Am J Pathol. 2013 Apr;182(4):1367-78.

Merli M, Giusto M, Molfino A, Bonetto A, Rossi M, Ginanni Corradini S, Baccino FM, Rossi

Fanelli F, Costelli P, Muscaritoli M. MuRF-1 and p-GSK3β expression in muscle atrophy of

liver cirrhosis. Liver Int. 2013 May;33(5): 714-21.

Penna F, Bonelli G, Baccino FM, Costelli P. Mechanism-based therapeutic approach to

cachexia. Vitam Horm. 2013;92:271-99.

Bonetto A, Penna F, Aversa Z, Mercantini P, Baccino FM, Costelli P, Ziparo V, Lucia S,

Rossi Fanelli F, Muscaritoli M. Early changes of muscle IGF-1 and myostatin gene

expression in gastric cancer patients. Muscle Nerve. 2013 Sep;48(3):387-92.

Minero VG, Khadjavi A, Costelli P, Baccino FM, Bonelli G. JNK activation is required for

TNFα-induced apoptosis in human hepatocarcinoma cells. Int Immunopharmacol. 2013

Sep;17(1):92-8.

Penna F, Busquets S, Toledo M, Pin F, Massa D, Lopez-Soriano FJ, Costelli P, Argiles JM.

Erythropoietin administration partially prevents adipose tissue loss in experimental cancer

cachexia models. J Lipid Res. 2013 Nov;54(11):3045-51.

Fontes-Oliveira CC, Busquets S, Toledo M, Penna F, Paz Aylwin M, Sirisi S, Silva AP, Orpí

M, García A, Sette A, Inês Genovese M, Olivan M, López-Soriano FJ, Argilés JM.

Mitochondrial and sarcoplasmic reticulum abnormalities in cancer cachexia: altered energetic

efficiency? Biochim Biophys Acta. 2013 Mar;1830(3):2770-8.

Faggi F, Mitola S, Sorci G, Riuzzi F, Donato R, Codenotti S, Poliani PL, Cominelli M,

Vescovi R, Rossi S, Calza S, Colombi M, Penna F, Costelli P, Perini I, Sampaolesi M, Monti

E, Fanzani A. Phosphocaveolin-1 enforces tumor growth and chemoresistance in

rhabdomyosarcoma. PLoS One. 2014 Jan 10;9(1):e84618.

Penna F, Baccino FM, Costelli P. Coming back: autophagy in cachexia. Curr Opin Clin Nutr

Metab Care. 2014 May;17(3):241-6.

Gallo S, Gatti S, Sala V, Albano R, Costelli P, Casanova E, Comoglio P, Crepaldi T. Agonist

antibodies activating the Met receptor protect cardiomyoblasts from cobalt chloride-induced

apoptosis and autophagy. Cell Death Dis. 2014 Apr 17;5:e1185.

Penna F, Bonetto A, Baccino FM, Costelli P. Glutamine and myostatin expression in muscle.

In Glutamine in Health and Disease, ed. R. Rajendram, V.R. Preedy, and V.B. Patel. 2014

London: Springer Press, in press.

27

GENERAL PATHOLOGY

LABORATORY FOR THE STUDY OF NUTRITION AND METABOLIC

DISORDERS

Prof. Manuela

ARAGNO Associate Professor

Dr. Raffaella

MASTROCOLA Research Technician

Dr. Debora

NIGRO PhD Student


Investigation on the molecular pathways underlying the pathogenesis of diet-induced

metabolic disorders such as obesity, dyslipidemia, insulin resistance and diabetes, focusing mainly

on the role of advanced glycation end products (AGEs) on the dysregulation of the lipogenic

pathway.

We recently developed in vivo models of diet-induced metabolic disorders for studying molecular

alterations occurring in various organs and tissue (including liver, kidney, heart, and skeletal

muscle).

We currently are the leading group of the following ongoing research projects:

- Role of the AGEs in the myosteatosis and muscle dysfunction in a high-fructose diet and

modulation by the anti-glycative compound pyridoxamine (in collaboration with Medana, Collino,

Minetto, D’Antona);

- Effects of a high-fat high-fructose diet in the impairment of recovery mechanisms and

activation of the inflammasome complex in the ischemic heart and prevention by a new anti-NFkB

activation compound (in collaboration with Pagliaro and Collino);

- Characterization of the lipid profile modifications in liver and plasma samples of high-fat or

high-fructose fed mice by different methods in GC-MS (in collaboration with Medana)

and we actively cooperate to the following studies:

- Efficacy of vitamin D supplementation in the prevention of obesity and insulin resistance in

high-fat high-sucrose diet fed mice (in collaboration with Collino, Minetto, D’Antona);

- Effects of the administration of innovative anti-inflammatory compounds in the diet-induced

insulin resistance (in collaboration with Collino and Thiemermann);

- Role of the NLRP3 inflammasome pathway in the development of diet-induced metabolic

derangements (in collaboration with Collino and Ryffel).

28


Medana C, Calza P, Giancotti V, Dal Bello F, Aragno M, Baiocchi C. Study of the

photocatalytic transformation of synephrine: a biogenic amine relevant in anti-doping

analysis. Anal Bioanal Chem. 2013 Jan;405(2-3):1105-13.

Collino M, Benetti E, Rogazzo M, Mastrocola R, Yaqoob MM, Aragno M, Thiemermann C,

Fantozzi R. Reversal of the deleterious effects of chronic dietary HFCS-55 intake by PPAR-δ

agonism correlates with impaired NLRP3 inflammasome activation. Biochem Pharmacol,

85(2):257-64, 2013.

Sansoè G, Aragno M, Mastrocola R, Paternostro C, Parola M. Calcium receptors located in

fibrotic septa: a new target to reduce portal pressure in liver cirrhosis. Clinical Science

125(2):67-75, 2013.

Mastrocola R, Collino M, Rogazzo M, Medana C, Nigro D, Boccuzzi G, Aragno M.

Advanced glycation end-products promote hepatosteatosis by interfering with SCAP-SREBP

system in fructose drinking mice. Am J Physiol – Gastrointestinal and Liver, 305(6):G398-

407, 2013

Benetti E, Mastrocola R, Rogazzo M, Chiazza F, Aragno M, Fantozzi R, Collino M and

Minetto MA. High Sugar Intake and Development of Skeletal Muscle Insulin Resistance and

Inflammation in Mice: A Protective Role for PPAR-𝛿 Agonism. Mediators of Inflammation,

2013:509502.

Collino M, Mastrocola R, Nigro D, Chiazza F, Aragno M, D'Antona G and Minetto MA.

Variability in myosteatosis and insulin resistance induced by high fat diet in mouse skeletal

muscles. Special issue "Nutrients and Muscle Disease”, BioMed Research International,

2014:56923.

Collino M, Benetti E, Rogazzo M, Chiazza F, Mastrocola R, Nigro D, Cutrin JC, Aragno M,

Fantozzi R, Minetto M, Thiemermann C. A nonerythropoietic peptide derivative of

erythropoietin decreases susceptibility to diet-induced insulin resistance in mice. Br J

Pharmacol 2014. In press

De Stefanis D, Mastrocola R, Nigro D, Costelli P, Aragno M. Effect of chronic sugar-diet on

mice skeletal muscle: a comparison between fructose and glucose. J Nutr 2014. Submitted.

Mastrocola R, Collino M, Nigro D, Chiazza F, D’Antona G, Aragno M, Minetto MA.

Advanced glycation end-products promote myosteatosis by activation of the SCAP/SREBP

lipogenetic pathway in mouse skeletal muscle. PlosOne 2014. Submitted

29

GENERAL PATHOLOGY AND PATHOPHYSIOLOGY

Prof. Giuseppe

POLI Full Professor

Prof. Elena

CHIARPOTTO Associate Professor

Prof. Fiorella

BIASI Associate Professor

Prof. Gabriella

LEONARDUZZI Associate Professor

Dr. Paola

GAMBA Assistant Professor

Dr. Simona

GARGIULO Postdoctoral

Fellowship

Dr. Gabriella

TESTA Postdoctoral

Fellowship

Dr. Marco

MAINA Postdoctoral

Fellowship

Dr. Tina

GUINA PhD Student

Dr. Simone

CALFAPIETRA PhD Student

Dr. Daniela

ROSSIN PhD Student

Dr. Barbara

SOTTERO Research Technician


Oxysterols, a family of 27-carbon molecules originated from cholesterol oxidation by both

enzymatic and non-enzymatic mechanisms, are comprehensively investigated for their potential

contribution to the pathogenesis and the progression of those human diseases that recognize

hypercholesterolemia as primary risk factor and are associated with inflammation. Among these

pathologies, atherosclerosis, Alzheimer’s disease, inflammatory bowel disease and colon

carcinogenesis are considered.

Very recently, much attention has also been drawn on the beneficial properties of oxysterols.

A few relevant examples are the ability of defined oxysterols to modulate physiologically

important nuclear receptors, induce survival signaling within cells and protect them against

infection by a broad variety of enveloped and non enveloped viruses.

30

Recent Publications (2013/2014) Vurusaner B, Gamba P, Testa G, Gargiulo S, Biasi F, Zerbinati C, Iuliano L, Leonarduzzi G,

Basaga H, Poli G. Survival signaling elicited by 27-hydroxycholesterol through the combined

modulation of cellular redox state and ERK/Akt phosphorylation. Free Radic. Biol. Med.

2014 Aug 7. [Epub ahead of print].

Biasi F, Deiana M, Guina T, Gamba P, Leonarduzzi G, Poli G. Wine consumption and

intestinal redox homeostasis. Redox Biol. 2014;2:795-802.

Poli G, Chiarpotto E. Editorial: lifestyle and therapeutics in the elderly. Curr. Pharm. Des.

2014;20:2949.

Testa G, Gamba P, Badilli U, Gargiulo S, Maina M, Guina T, Calfapietra S, Biasi F, Cavalli

R, Poli G, Leonarduzzi G. Loading into nanoparticles improves quercetin's efficacy in

preventing neuroinflammation induced by oxysterols. PLoS One 2014;9(5):e96795.

Poli G. A new biochemical effect of 4-hydroxynonenal: downregulation of TGFβ-dependent

elastogenesis. Free Radic. Biol. Med. 2014;71:437-438.

Gamba P, Guglielmotto M, Testa G, Monteleone D, Zerbinati C, Gargiulo S, Biasi F, Iuliano

L, Giaccone G, Mauro A, Poli G, Tamagno E, Leonarduzzi G. Up-regulation of β-

amyloidogenesis in neuron-like human cells by both 24- and 27-hydroxycholesterol:

protective effect of N-acetyl-cysteine. Aging Cell 2014;13:561-572.

Vizio B, Biasi F, Scirelli T, Novarino A, Prati A, Ciuffreda L, Montrucchio G, Poli G,

Bellone G. Pancreatic-carcinoma-cell-derived pro-angiogenic factors can induce endothelial-

cell differentiation of a subset of circulating CD34+ progenitors. J. Transl. Med. 2013;11:314.

Goitre L, De Luca E, Braggion S, Trapani E, Guglielmotto M, Biasi F, Forni M, Moglia A,

Trabalzini L, Retta SF. KRIT1 loss of function causes a ROS-dependent upregulation of c-

Jun. Free Radic. Biol. Med. 2014;68:134-147.

Testa G, Biasi F, Poli G, Chiarpotto E. Calorie restriction and dietary restriction mimetics: a

strategy for improving healthy aging and longevity. Curr. Pharm. Des. 2014;20:2950-2977.

Gargiulo S, Gamba P, Poli G, Leonarduzzi G. Metalloproteinases and metalloproteinase

inhibitors in age-related diseases. Curr. Pharm. Des. 2014;20:2993-3018.

Poli G, Biasi F, Leonarduzzi G. Oxysterols in the pathogenesis of major chronic diseases.

Redox Biol. 2013;1:125-130.

Biasi F, Guina T, Maina M, Cabboi B, Deiana M, Tuberoso CI, Calfapietra S, Chiarpotto E,

Sottero B, Gamba P, Gargiulo S, Brunetto V, Testa G, Dessì MA, Poli G, Leonarduzzi G.

Phenolic compounds present in Sardinian wine extracts protect against the production of

inflammatory cytokines induced by oxysterols in CaCo-2 human enterocyte-like cells.

Biochem. Pharmacol. 2013;86:138-145.

Biasi F, Leonarduzzi G, Oteiza PI, Poli G. Inflammatory bowel disease: mechanisms, redox

considerations, and therapeutic targets. Antioxid. Redox Signal. 2013;19:1711-1747.

Biasi F, Chiarpotto E, Sottero B, Maina M, Mascia C, Guina T, Gamba P, Gargiulo S, Testa

G, Leonarduzzi G, Poli G. Evidence of cell damage induced by major components of a diet-

31

compatible mixture of oxysterols in human colon cancer CaCo-2 cell line. Biochimie

2013;95:632-640.

Pizzimenti S, Ciamporcero E, Pettazzoni P, Osella-Abate S, Novelli M, Toaldo C, Husse M,

Daga M, Minelli R, Bisazza A, Ferruti P, Ranucci E, Grazia Bernengo M, Dianzani C, Biasi

F, Cavalli R, Barrera G. The inclusion complex of 4-hydroxynonenal with a polymeric

derivative of β-cyclodextrin enhances the antitumoral efficacy of the aldehyde in several

tumor cell lines and in a three-dimensional human melanoma model. Free Radic. Biol. Med.

2013;65:765-777.

Penna F, Costamagna D, Pin F, Camperi A, Fanzani A, Chiarpotto EM, Cavallini G, Bonelli

G, Baccino FM, Costelli P. Autophagic degradation contributes to muscle wasting in cancer

cachexia. Am. J. Pathol. 2013;182:1367-1378.

Lezo A, Biasi F, Massarenti P, Calabrese R, Poli G, Santini B, Bignamini E. Oxidative stress

in stable cystic fibrosis patients: do we need higher antioxidant plasma levels? J. Cyst.

Fibrosis 2013;12:35-41.

32

HEMATOLOGY AND STEM CELL TRANSPLANTATION LABORATORY

Prof. Daniela

CILLONI Associate Professor

Dr. Valentina

ROSSO Postdoctoral

Fellowship

Dr. Sonia

CARTURAN Postdoctoral

Fellowship

Dr. Jessica

PETITI PhD Student

Dr. Elisabetta

SIGNORINO PhD Student


Molecular characterization of myeloproliferative disorders and myelodysplastic syndromes

including the development of drosophila model for the genetic characterization of oncogenic

pathways.

Study of stemness properties and aging of cord blood and adult stem cells before and after

transplantation

Clonal evolution and markers of Acute Myeloid Leukemia.

33


Efficace F, Santini V, La Nasa G, Cottone F, Finelli C, Borin L, Quaresmini G, Di Tucci AA,

Volpe A, Cilloni D, Quarta G, Sanpaolo G, Rivellini F, Salvi F, Molteni A, Voso MT,

Alimena G, Fenu S, Mandelli F, Angelucci E. Health-relatedquality of life in transfusion-

dependent patients with myelodysplastic syndromes:a prospective study to assess the impact

of iron chelation therapy. BMJ Support Palliat Care. 2014 Sep 9 2014

Aslanyan MG, Kroeze LI, Langemeijer SM, Koorenhof-Scheele TN, Massop M, vanHoogen

P, Stevens-Linders E, van de Locht LT, Tönnissen E, van der Heijden A, da Silva-Coelho P,

Cilloni D, Saglio G, Marie JP, Tang R, Labar B, Amadori S, MuusP, Willemze R, Marijt EW,

de Witte T, van der Reijden BA, Suciu S, Jansen JH.Clinical and biological impact of TET2

mutations and expression in younger adult AML patients treated within the

EORTC/GIMEMA AML-12 clinical trial. Ann Hematol. 2014 Aug;93(8):1401-12

Angelucci E, Santini V, Di Tucci AA, Quaresmini G, Finelli C, Volpe A, Quarta G, Rivellini

F, Sanpaolo G, Cilloni D, Salvi F, Caocci G, Molteni A, Vallisa D, Voso MT, Fenu S, Borin

L, Latte G, Alimena G, Storti S, Piciocchi A, Fazi P, Vignetti M, Tura S. Deferasirox for

transfusion-dependent patients with myelodysplastic syndromes: safety, efficacy, and beyond

(GIMEMA MDS0306 Trial). Eur J Haematol. 2014 Jun;92(6):527-36

Willemze R, Suciu S, Meloni G, Labar B, Marie JP, Halkes CJ, Muus P, Mistrik M, Amadori

S, Specchia G, Fabbiano F, Nobile F, Sborgia M, Camera A, Selleslag DL, Lefrère F Sr,

Magro D, Sica S, Cantore N, Beksac M, Berneman Z, Thomas X, Melillo L, Guimaraes JE,

Leoni P, Luppi M, Mitra ME, Bron D, Fillet G, Marijt EW, Venditti A, Hagemeijer A,

Mancini M, Jansen J, Cilloni D, Meert L, Fazi P, Vignetti M, Trisolini SM, Mandelli F, de

Witte T. High-dose cytarabine in induction treatment improves the outcome of adult patients

younger than age 46 years with acute myeloid leukemia: results of the EORTC-GIMEMA

AML-12 trial. J Clin Oncol. 2014 Jan 20;32(3):219-28.

Frassoni F, Cilloni D, Podestà M. New possibilities to exploit the potentiality of cord blood

cells in the context of transplantation. Immunol Lett. 2013 Sep-Oct;155(1-2):24-6.

Bracco E, Rosso V, Serra A, Carnuccio F, Gaidano V, Nicoli P, Musto P, Saglio G, Frassoni

F, Cilloni D. Design and application of a novel PNA probe for thedetection at single cell level

of JAK2V617F mutation in MyeloproliferativeNeoplasms. BMC Cancer. 2013 Jul 18;13:348.

Finazzi G, Vannucchi AM, Martinelli V, Ruggeri M, Nobile F, Specchia G, Pogliani EM,

Olimpieri OM, Fioritoni G, Musolino C, Cilloni D, Sivera P, BarosiG, Finazzi MC, Di Tollo

S, Demuth T, Barbui T, Rambaldi A. A phase II study of Givinostat in combination with

hydroxycarbamide in patients with polycythaemiavera unresponsive to hydroxycarbamide

monotherapy. Br J Haematol. 2013Jun;161(5):688-94

Cilloni D, Carturan S, Bracco E, Campia V, Rosso V, Torti D, Calabrese C, Gaidano V,

Niparuck P, Favole A, Signorino E, Iacobucci I, Morano A, De Luca L,Musto P, Frassoni F,

Saglio G. Aberrant activation of ROS1 represents a newmolecular defect in chronic

myelomonocytic leukemia. Leuk Res. 2013May;37(5):520-30.

Marchioli R, Finazzi G, Specchia G, Cacciola R, Cavazzina R, Cilloni D, De Stefano V, Elli

E, Iurlo A, Latagliata R, Lunghi F, Lunghi M, Marfisi RM, Musto P, Masciulli A, Musolino

C, Cascavilla N, Quarta G, Randi ML, Rapezzi D, Ruggeri M, Rumi E, Scortechini AR,

34

Santini S, Scarano M, Siragusa S, Spadea A, Tieghi A, Angelucci E, Visani G, Vannucchi

AM, Barbui T; CYTO-PV Collaborative Group.Cardiovascular events and intensity of

treatment in polycythemia vera. N Engl JMed. 2013 Jan 3;368(1):22-33.

Angelini S, Soverini S, Ravegnini G, Barnett M, Turrini E, Thornquist M, PaneF, Hughes TP,

White DL, Radich J, Kim DW, Saglio G, Cilloni D, Iacobucci I,Perini G, Woodman R,

Cantelli-Forti G, Baccarani M, Hrelia P, Martinelli G.Association between imatinib

transporters and metabolizing enzymes genotype andresponse in newly diagnosed chronic

myeloid leukemia patients receiving imatinib therapy. Haematologica. 2013 Feb;98(2):193-

200.

35

HUMAN ANATOMY

Prof. Maria G.

ROBECCHI Full Professor

Prof. Stefano

GEUNA Associate Professor

Dr. Stefania

RAIMONDO Assistant Professor

Dr. Federica

FREGNAN Postdoctoral

Fellowship

Dr. Sara

GNAVI Postdoctoral

Fellowship

Dr. Luisa

MURATORI

PhD Student

Dr. Davide

PASCAL PhD Student

Dr. Michela

MORANO PhD Student


The current research projects are focused in five main directions:

to investigate, by means of an interdisciplinary approach, innovative strategies for the tissue

engineering of the damaged peripheral nerves;

to investigate the neuronal adaptive changes occurring after peripheral nerve trauma and/or

disease;

to investigate innovative strategies to prevent skeletal muscle atrophy after peripheral nerve

damage;

to investigate the neuroendocrine mechanisms involved in peripheral nerve regeneration;

to develop new behavioral and stereological protocols for reliable quantitative assessment of the

PNS.

36


Novajra G, Tonda-Turo C, Vitale-Brovarone C, Ciardelli G, Geuna S, Raimondo S. “Novel

systems for tailored neurotrophic factor release based on hydrogel and resorbable glass

hollow fibers.” Mater Sci Eng C Mater Biol Appl. 36:25-32 (2014).

Buskbjerg Jager S, Ronchi G, Bjerggaard Vaegter C, Geuna S. “The mouse median nerve

experimental model in regenerative research.” Biomed Res Int. 2014:701682 (2014).

Beck-Broichsitter BE, Lamia A, Geuna S, Fregnan F, Smeets R, Becker ST, Sinis N. “Does

pulsed magnetic field therapy influence nerve regeneration in the median nerve model of the

rat?” Biomed Res Int. 2014:401760 (2014).

Ziv-Polat O, Shahar A, Levy I, Skaat H, Neuman S, Fregnan F, Geuna S, Grothe C, Haastert-

Talini K, Margel S. “The role of neurotrophic factors conjugated to iron oxide nanoparticles

in peripheral nerve regeneration: in vitro studies.” Biomed Res Int. 2014:267808 (2014).

Riccio M, Pangrazi PP, Parodi PC, Vaienti L, Marchesini A, Neuendorf AD, Bottegoni C,

Tos P, Geuna S. “The amnion muscle combined graft (AMCG) conduits: A new alternative in

the repair of wide substance loss of peripheral nerves”. Microsurgery. (2014).

Dimauro I, Grasso L, Fittipaldi S, Fantini C, Mercatelli N, Racca S, Geuna S, Di

Gianfrancesco A, Caporossi D, Pigozzi F, Borrione P. “Platelet-rich plasma and skeletal

muscle healing: a molecular analysis of the early phases of the regeneration process in an

experimental animal model.” PLoS One. 9(7):e102993 (2014).

Crosio A, Valdatta L, Cherubino M, Izzo M, Pellegatta I, Pascal D, Geuna S, Tos P. “A

simple and reliable method to perform biomechanical evaluation of postoperative nerve

adhesions.” J Neurosci Methods. 233:73-7 (2014).

Papalia I, Raimondo S, Ronchi G, Magaudda L, Giacobini-Robecchi MG, Geuna S.

“Repairing nerve gaps by vein conduits filled with lipoaspirate-derived entire adipose tissue

hinders nerve regeneration.” Ann Anat. 195(3):225-30 (2013)

Geuna S, Gnavi S, Perroteau I, Tos P, Battiston B. Tissue engineering and peripheral nerve

reconstruction: an overview.

Int Rev Neurobiol 108:35-57 (2013).

Haastert-Talini K, Geuna S, Dahlin LB, Meyer C, Stenberg L, Freier T, Heimann C, Barwig

C, Pinto LF, Raimondo S, Gambarotta G, Samy SR, Sousa N, Salgado AJ, Ratzka A, Wrobel

S, Grothe C. Chitosan tubes of varying degrees of acetylation for bridging peripheral nerve

defects. Biomaterials 34:9886-904 (2013).




atrophy. Gene Ther 20:1014-21 (2013).

37

Ronchi G, Gambarotta G, Di Scipio F, Salamone P, Sprio AE, Cavallo F, Perroteau I, Berta

GN, Geuna S. ErbB2 receptor over-expression improves post-traumatic peripheral nerve

regeneration in adult mice. PLoS One 8(2):e56282 (2013).

Costa LM, Pereira JE, Filipe VM, Magalhães LG, Couto PA, Gonzalo-Orden JM, Raimondo

S, Geuna S, Maurício AC, Nikulina E, Filbin MT, Varejão AS. Rolipram promotes functional

recovery after contusive thoracic spinal cord injury in rats. Behav Brain Res 243:66-73

(2013).

Papalia I, Raimondo S, Ronchi G, Magaudda L, Giacobini-Robecchi MG, Geuna S. Repairing

nerve gaps by vein conduits filled with lipoaspirate-derived entire adipose tissue hinders

nerve regeneration. Ann Anat 195:225-30 (2013).



I, Smith RG, Sun Y, Geuna S, Graziani A. Acylated and unacylated ghrelin impair skeletal

muscle atrophy in mice. J Clin Invest 123:611-22 (2013).

38

HUMAN AND INDUSTRIAL TOXICOLOGY

Dr. Giovanni

MAINA

Assistant Professor

Dr. Andrea Elio

SPRIO Postdoctoral

Fellowship

Dr. Lisa

ACCOMASSO

Postdoctoral

Fellowship


The current research projects are focused in two main directions:

to prepare, and to evaluate formulations of drug incorporated in new types of nano-vehicles

(cyclodextrin-based nanosponges and micro and nanobubbles).

to develop technical textile materials with innovative and highly durable antibacterial activities for

civil and aerospace applications.

39


Miola M, Bruno M, Maina G, Fucale G, Lucchetta G, Vernè E. “Antibiotic-free composite bone

cements with antibacterial and bioactive properties. A preliminary study.” Mater Sci Eng C

Mater Biol Appl. 43:65-75. (2014)

Miola M, Brovarone CV, Maina G, Rossi F, Bergandi L, Ghigo D, Saracino S, Maggiora M,

Canuto RA, Muzio G, Vernè E. “In vitro study of manganese-doped bioactive glasses for bone

regeneration.” Mater Sci Eng C Mater Biol Appl. 38:107-18. (2014)

Bianco C, Adami G, Crosera M, Larese F, Casarin S, Castagnoli C, Stella M, Maina G. “Silver

percutaneous absorption after exposure to silver nanoparticles: A comparison study of three

human skin graft samples used for clinical applications.” Burns. 40(7):1390-6. (2014)

Dianzani C, Zara GP, Maina G, Pettazzoni P, Pizzimenti S, Rossi F, Gigliotti CL, Ciamporcero

ES, Daga M, Barrera G. “Drug delivery nanoparticles in skin cancers.” Biomed Res Int.

2014:895986. (2014)

Larese Filon F, Crosera M, Timeus E, Adami G, Bovenzi M, Ponti J, Maina G. “Human skin

penetration of cobalt nanoparticles through intact and damaged skin.” Toxicol In Vitro.

27(1):121-7. (2013)

40

MEDICAL GENETICS

HUMAN GENETICS GROUP

Prof. Claudia

GIACHINO Associate Professor

Dr. Silvia

SAVIOZZI Research Technician

Dr. Valentina

TURINETTO Postdoctoral

Fellowship

Dr. Clara

GALLINA PhD Student

Dr Tania

CAPELÔA PhD Student


Our current research projects are focused on four main areas:

Genetic and epigenetic stability of stem cells;

Medical devices for innovative therapies (In vivo tissue engineering for myocardium

regeneration);

Bio-nano-technologies for regenerative medicine (biocompatibility and biosafety of micro

and nanoparticles, stem cell imaging).

DNA repair capacity and cancer risk.

41


Minieri V, Saviozzi S, Gambarotta G, Lo Iacono M, Accomasso L, Cibrario Rocchietti E,

Gallina C, Turinetto V, Giachino C. Persistent DNA damage-induced premature senescence

alters the functional features of human bone marrow mesenchymal stem cells. J Cell Mol

Med. 2014, in press.

Pardini B, Allione A, Guarrera S, Turinetto V, Fiorito G, Rosa F, Russo A, Modica F,

Giachino C, Vineis P, Sacerdote C, Matullo G. DNA repair capacity, gene expression and

epigenomic profiles in bladder cancer. Cancer Res. 2014 Oct; 74 (19 Suppl):5367.

Lo Iacono M, Monica V, Vavalà T, Gisabella M, Saviozzi S, Bracco E, Novello S, Papotti M,

Vittorio Scagliotti G. ATF2 contributes to cisplatin resistance in Non-Small cell lung cancer

and Celastrol induces cisplatin re-sensitization through inhibition of JNK/ATF2 pathway. Int

J Cancer. 2014 Oct 30.


P, Rastaldo R, Raimondo S, Saviozzi S, Sprio AE, Gagliardi M, Barbani N, Giachino C. The


on stem cell commitment towards cardiac lineage. Biomaterials. 2014 Jan;35(1):92-104.


P, Rastaldo R, Raimondo S, Saviozzi S, Sprio A, Barbani N, Giachino C. The guidance of

stem cell cardiomyogenic differentiation by bioartificial scaffolds mimicking myocardium

structure and biomechanics. EPMA Journal 2014, 5(Suppl 1):A81.

Fornero S, Bassino E, Ramella R, Gallina C, Mahata SK, Tota B, Levi R, Alloatti G, Gallo

MP. Obligatory role for endothelial heparan sulphate proteoglycans and caveolae

internalization in catestatin-dependent eNOS activation. Biomed Res Int. 2014; 2014:783623.

Capelôa T, Caramelo F, Fontes-Ribeiro C, Gomes C, Silva AP. Role of methamphetamine on

glioblastoma cytotoxicity induced by doxorubicin and methotrexate. Neurotox Res. 2014

Oct;26(3):216-27.

Allione A, Porcedda P, Russo A, Ricceri F, Simonelli V, Minoprio A, Guarrera S, Pardini B,

Mazzei F, Dogliotti E, Giachino C, Matullo G. Effect of blood storage conditions on DNA

repair capacity measurements in peripheral blood mononuclear cells. Mutat Res. 2013

Sep;749(1-2):73-9.

Giachino C, Orlando L, Turinetto V. Maintenance of genomic stability in mouse embryonic

stem cells: relevance in aging and disease. Int J Mol Sci. 2013 Jan 28;14(2):2617-36.

42

Research Team

www.unito.it/dscb

HUMAN PHYSIOLOGY

(CARDIOVASCULAR PHYSIOLOGY)

Prof. Pasquale

PAGLIARO Associate Professor

Dr. Caludia

PENNA Assistant Professor

Dr. Daniele

MANCARDI Assistant Professor

Dr. Raffaella

RASTALDO Assistant Professor

Dr. Maria Giulia

PERRELLI Postdoctoral

Fellowship

Dr. Francesca

TULLIO Postdoctoral

Fellowship

Dr. Anna

FOLINO Postdoctoral

Fellowship

Dr. Carmelina

ANGOTTI PhD Student

Dr. Saveria

FEMMINÒ PhD Student


The current research projects are focused on three main areas:

Cardio-protection and cardio-regeneration: to study novel cardioprotective strategies

elicited by peptides (chromogranine and derivates, apelin, bradykinin) and to study the

importance of microstructure for stem cell orientation and differentiation of functionalized

cardioregenerative and cardioprotective biomaterials;

Stem cells and regenerative medicine: pluripotent cardiac stem cell, adult mesenchymal

stem cells and induced pluripotent stem cell cultures;

Molecular and Cellular Biology: mitochondrial redox signaling and stress, cardioprotective

signaling pathways; gas transmitters and calcium signaling.

By coupling the use of relevant experimental models (with comorbidities and with concomitant best

therapies) to further knowledge of endogenous cardioprotective pathways, we hope to identify

appropriate candidates (cells, substances and/or biomaterials) for each cardioprotective-

cardioregenerative strategy to be tested in future clinical trials and one day defeat ischemic disease.

43

Research products

www.unito.it/dscb


Altaany Z, Moccia F, Munaron L, Mancardi D, Wang R. Hydrogen Sulfide and Endothelial

Dysfunction: Relationship with Nitric Oxide. Curr Med Chem. 2014 Jul 6. [Epub ahead of

print] PubMed PMID: 25005182.

Avanzato D, Merlino A, Porrera S, Wang R, Munaron L, Mancardi D. Role of calcium

channels in the protective effect of hydrogen sulfide in rat cardiomyoblasts. Cell Physiol

Biochem. 2014;33(4):1205-14.).


P, Rastaldo R, Raimondo S, Saviozzi S, Sprio AE, Gagliardi M, Barbani N, Giachino C. The


on stem cell commitment towards cardiac lineage. Biomaterials. 2014 Jan;35(1):92-104.

Di Scipio F, Sprio AE, Folino A, Carere ME, Salamone P, Yang Z, Berrone M, Prat M,

Losano G, Rastaldo R, Berta GN. Injured cardiomyocytes promote dental pulp mesenchymal

stem cell homing. Biochim Biophys Acta. 2014 Jul;1840(7):2152-61.

Folino A, Losano G, Rastaldo R. Balance of nitric oxide and reactive oxygen species in

myocardial reperfusion injury and protection. J Cardiovasc Pharmacol. 2013 Dec;62(6):567-

75.

Mognetti B, La Montagna G, Perrelli MG, Pagliaro P, Penna C. Bone marrow mesenchymal

stem cells increase motility of prostate cancer cells via production of stromal cell-derived

factor-1α. J Cell Mol Med. 2013 Feb;17(2):287-92.

Munaron L, Avanzato D, Moccia F, Mancardi D. Hydrogen sulfide as a regulator of calcium

channels. Cell Calcium. 2013 Feb;53(2):77-84.

Pagliaro P, Gattullo D, Penna C. Nitroglycerine and sodium trioxodinitrate: from the

discovery to the preconditioning effect. J Cardiovasc Med (Hagerstown). 2013

Oct;14(10):698-704.

Pagliaro P, Penna C. Redox signaling and cardioprotection – translatability and mechanism.

Br J Pharmacol. 2014 Oct 10.

Penna C, Angotti C, Pagliaro P. Protein S-nitrosylation in preconditioning and

postconditioning. Exp Biol Med (Maywood). 2014 Mar 25;239(6):647-662.

Penna C, Brancaccio M, Tullio F, Rubinetto C, Perrelli MG, Angotti C, Pagliaro P, Tarone G.

Overexpression of the muscle-specific protein, melusin, protects from cardiac

ischemia/reperfusion injury. Basic Res Cardiol. 2014 Jul;109(4):418.

Penna C, Pasqua T, Amelio D, Perrelli MG, Angotti C, Tullio F, Mahata SK, Tota B, Pagliaro

P, Cerra MC, Angelone T. Catestatin increases the expression of anti-apoptotic and pro-

angiogenetic factors in the post-ischemic hypertrophied heart of SHR. PLoS One. 2014 Aug

6;9(8):e102536. eCollection 2014.

Penna C, Perrelli MG, Karam JP, Angotti C, Muscari C, Montero-Menei CN, Pagliaro P.

Pharmacologically active microcarriers influence VEGF-A effects on mesenchymal stem cell

survival. J Cell Mol Med. 2013 Jan;17(1):192-204.

44

Research Team

www.unito.it/dscb

Penna C, Perrelli MG, Pagliaro P. Mitochondrial pathways, permeabilità transition pore, and

redox signaling in cardioprotection: therapeutic implications. Antioxid Redox Signal. 2013

Feb 10;18(5):556-99.

Penna C, Perrelli MG, Tullio F, Angotti C, Camporeale A, Poli V, Pagliaro P. Diazoxide

postconditioning induces mitochondrial protein S-nitrosylation and a redox-sensitive

mitochondrial phosphorylation/translocation of RISK elements: no role for SAFE. Basic Res

Cardiol. 2013 Sep;108(5):371.

Penna C, Perrelli MG, Tullio F, Angotti C, Pagliaro P. Acidic infusion in early reperfusion

affects the activity of antioxidant enzymes in postischemic isolated rat heart. J Surg Res. 2013

Jul;183(1):111-8.

Penna C, Settanni F, Tullio F, Trovato L, Pagliaro P, Alloatti G, Ghigo E, Granata R. GH-

releasing hormone induces cardioprotection in isolated male rat heart via activation of RISK

and SAFE pathways. Endocrinology. 2013 Apr;154(4):1624-35.

Perrelli MG, Tullio F, Angotti C, Cerra MC, Angelone T, Tota B, Alloatti G, Penna C,

Pagliaro P. Catestatin reduces myocardial ischaemia/reperfusion injury: involvement of

PI3K/Akt, PKCs, mitochondrial KATP channels and ROS signalling. Pflugers Arch. 2013

Jul;465(7):1031-40.

Tullio F, Angotti C, Perrelli MG, Penna C, Pagliaro P. Redox balance and cardioprotection.

Basic Res Cardiol. 2013 Nov;108(6):392.

45

INTERNAL MEDICINE 3 - METABOLIC DISEASE UNIT

Prof. Angelo

GUERRASIO Associate Professor

Dr. Franco

CAVALOT Medical Doctor

Dr. Isabella

RUSSO Research Technician

Mr. Luigi

MATTIELLO Research Technician

Dr. Cristina

BARALE PhD Student


Glucagon-like peptide -1 effects on platelet function in health, obesity and Type 2 Diabetes Mellitus

Extracellular depletion and overload of iron on platelet responses in in vitro and in vivo models

46

Recent Publications (2013/2014) Russo I, Viretto M, Doronzo G, Barale C, Mattiello L, Anfossi G, Trovati M. A short-term

incubation with high glucose impairs VASP phosphorylation at serine 239 in response to the

nitric oxide/cGMP pathway in vascular smooth muscle cells: role of oxidative stress. Biomed

Res Int. 2014;2014:328959.

Galluccio E, Cassina L, Russo I, Gelmini F, Setola E, Rampoldi L, Citterio L, Rossodivita A,

Kamami M, Colombo A, Alfieri O, Carini M, Bosi E, Trovati M, Piatti P, Monti LD, Casari

G. A novel truncated form of eNOS associates with altered vascular function. Cardiovasc

Res. 2014 Mar 1;101(3):492-502.

Trovati M, Doronzo G, Barale C, Vaccheris C, Russo I, Cavalot F. Leptin and vascular

smooth muscle cells. Curr Pharm Des. 2014;20(4):625-34.

Doronzo G, Viretto M, Barale C, Russo I, Mattiello L, Anfossi G, Trovati M. Oleic acid

increases synthesis and secretion of VEGF in rat vascular smooth muscle cells: role of

oxidative stress and impairment in obesity. Int J Mol Sci. 2013 Sep 13;14(9):18861-80.

47

INTERNAL MEDICINE I

Prof. Massimo

TERZOLO Associate Professor

Dr. Giuseppe

REIMONDO Assistant Professor

Dr. Anna

PIA Medical Doctor

Dr. Fulvia C.

DAFFARA Medical Doctor

Dr. Marcella

COLETTA Medical Doctor,

Fellowship

Dr. Vittoria

BASILE Medical Doctor

Dr. Barbara

ZAGGIA Medical Doctor

Dr. Chiara

SCIOLLA Fellowship

Dr. Laura

SABA Research Technician

Dr. Antonina

GERMANO Fellowship

Dr. Angela

TERMINE Research Technician

Dr. Paola

PEROTTI Data manager

Fiorenza

CATINO Secretary


The current research projects of Prof. Terzolo’s group are mainly related to two main topics and

concern either preclinical or clinical aspects:

benign adrenal tumors;

malignant adrenal tumors (adrenocortical carcinoma, ACC).

Research projects on the first area involve analysis of a number of polymorphisms of the

glucocorticoid receptor gene in patients with adrenal incidentaloma; definition of management and

follow-up strategies of subclinical hypercortisolism; evaluation of biomarkers predictive of

peripheral cortisol effect; assessment of the natural history of adrenal incidentalomas.

48

Research projects in the second area involve analysis of polymorphisms of mitochondrial

enzymes on the pharmacokinetics of mitotane, the reference treatment for ACC; in vitro

assessment of the cytotoxic activity of different drugs (such as mitotane and gemcitabine)

in ACC cell lines; assessment of markers predictive of efficacy for adjuvant mitotane

and of prognostic markers in ACC; assessment of novel diagnostic and therapeutic tools

for ACC patients.

49


Baratelli C, Brizzi MP, Tampellini M, Scagliotti GV, Priola A, Terzolo M, Pia A, Berruti A.

Intermittent everolimus administration for malignant insulinoma. Endocrinol Diabetes Metab

Case Rep. 2014;2014:140047.

Ronchi CL, Sbiera S, Volante M, Steinhauer S, Scott-Wild V, Altieri B, Kroiss M, Bala M,

Papotti M, Deutschbein T, Terzolo M, Fassnacht M, Allolio B. CYP2W1 is highly expressed

in adrenal glands and is positively associated with the response to mitotane in adrenocortical

carcinoma. PLoS One. 2014 Aug 21;9(8):e105855.

Duregon E, Rapa I, Votta A, Giorcelli J, Daffara F, Terzolo M, Scagliotti GV, Volante M,

Papotti M. MicroRNA expression patterns in adrenocortical carcinoma variants and clinical

pathologic correlations. Hum Pathol. 2014 Aug;45(8):1555-62.

Terzolo M, Zaggia B, Allasino B, De Francia S. Practical treatment using mitotane for

adrenocortical carcinoma. Curr Opin Endocrinol Diabetes Obes. 2014 Jun;21(3):159-65.

Duregon E, Molinaro L, Volante M, Ventura L, Righi L, Bolla S, Terzolo M, Sapino A,

Papotti MG. Comparative diagnostic and prognostic performances of the hematoxylin-eosin

and phospho-histone H3 mitotic count and Ki-67 index in adrenocortical carcinoma. Mod

Pathol. 2014 Sep;27(9):1246-54.

Terzolo M, Allasino B, Pia A, Peraga G, Daffara F, Laino F, Ardito A, Termine A, Paccotti P,

Berchialla P, Migliaretti G, Reimondo G. Surgical Remission of Cushing's syndrome reduces

cardiovascular risk. Eur J Endocrinol. 2014 Jul;171(1):127-36. Epub 2014 May 6.

Morelli V, Reimondo G, Giordano R, Della Casa S, Policola C, Palmieri S, Salcuni AS, Dolci

A, Mendola M, Arosio M, Ambrosi B, Scillitani A, Ghigo E, Beck- Peccoz P, Terzolo M,

Chiodini I. Long-term follow-up in adrenal incidentalomas: an Italian multicenter study. J

Clin Endocrinol Metab. 2014 Mar;99(3):827-34.

Berruti A, Fassnacht M, Haak H, Else T, Baudin E, Sperone P, Kroiss M, Kerkhofs T,

Williams AR, Ardito A, Leboulleux S, Volante M, Deutschbein T, Feelders R, Ronchi C,

Grisanti S, Gelderblom H, Porpiglia F, Papotti M, Hammer GD, Allolio B, Terzolo M.

Prognostic role of overt hypercortisolism in completely operated patients with adrenocortical

cancer. Eur Urol. 2014 Apr;65(4):832-8.

Germano A, Rapa I, Volante M, Lo Buono N, Carturan S, Berruti A, Terzolo M, Papotti M.

Cytotoxic activity of gemcitabine, alone or in combination with mitotane, in adrenocortical

carcinoma cell lines. Mol Cell Endocrinol. 2014 Jan 25;382(1):1-7.

Gentilin E, Tagliati F, Terzolo M, Zoli M, Lapparelli M, Minoia M, Ambrosio MR, Degli

Uberti EC, Zatelli MC. Mitotane reduces human and mouse ACTH-secreting pituitary cell

viability and function. J Endocrinol. 2013 Jul 29;218(3):275-85.

Reimondo G, Bondanelli M, Ambrosio MR, Grimaldi F, Zaggia B, Zatelli MC, Allasino B,

Laino F, Aroasio E, Termine A, Conton P, Paoletta A, Demenis E, Uberti ED, Terzolo M.

Growth hormone values after an oral glucose load do not add clinically useful information in

patients with acromegaly on long-term somatostatin receptor ligand treatment. Endocrine.

2014 Feb;45(1):122-7.

50

Duregon E, Fassina A, Volante M, Nesi G, Santi R, Gatti G, Cappellesso R, Dalino

Ciaramella P, Ventura L, Gambacorta M, Dei Tos AP, Loli P, Mannelli M, Mantero F, Berruti

A, Terzolo M, Papotti M. The reticulin algorithm for adrenocortical tumor diagnosis: a

multicentric validation study on 245 unpublished cases. Am J Surg Pathol. 2013

Sep;37(9):1433-40.

Pia A, Vignani F, Attard G, Tucci M, Bironzo P, Scagliotti G, Arlt W, Terzolo M, Berruti A.

Strategies for managing ACTH dependent mineralocorticoid excess induced by abiraterone.

Cancer Treat Rev. 2013 Dec;39(8):966-73.

D'Avolio A, De Francia S, Basile V, Cusato J, De Martino F, Pirro E, Piccione F, Ardito A,

Zaggia B, Volante M, Di Perri G, Terzolo M. Influence of the CYP2B6 polymorphism on the

pharmacokinetics of mitotane. Pharmacogenet Genomics. 2013 Jun;23(6):293-300.

Chortis V, Taylor AE, Schneider P, Tomlinson JW, Hughes BA, O'Neil DM, Libé R, Allolio

B, Bertagna X, Bertherat J, Beuschlein F, Fassnacht M, Karavitaki N, Mannelli M, Mantero

F, Opocher G, Porfiri E, Quinkler M, Sherlock M, Terzolo M, Nightingale P, Shackleton CH,

Stewart PM, Hahner S, Arlt W. Mitotane therapy in adrenocortical cancer induces CYP3A4

and inhibits 5α-reductase, explaining the need for personalized glucocorticoid and androgen

replacement. J Clin Endocrinol Metab. 2013 Jan;98(1):161-71.

Duregon E, Volante M, Giorcelli J, Terzolo M, Lalli E, Papotti M. Diagnostic and prognostic

role of steroidogenic factor 1 in adrenocortical carcinoma: a validation study focusing on

clinical and pathologic correlates. Hum Pathol. 2013 May;44(5):822-8.

51

IRON METABOLISM

Prof. Giuseppe

SAGLIO Full Professor

Dr. Antonella

ROETTO Research Technician

Dr.Rosa Maria

PELLEGRINO PhD Student

Dr. Martina

BOERO PhD Student

Dr. Antonietta

PALMIERI Fellowship

Dr. Ludovica

FERBO Specialization

Student

Dr. Carmen

FAVA Assistant Professor


The team studies the genetic basis of iron homeostasis trying to elucidate in particular the role of

TFR2 in iron metabolism utilizing Tfr2 mouse models.

The Transferrin receptor 2 (TFR2) gene codifies for an iron sensor protein and is involved in the

pathogenesis of iron overload genetic disorder named haemochromatosis type 3 (HFE3) Two

alternative transcripts of TFR2 have been reported. Tfr2 proteins, highly expressed in the liver,

acts regulating the hepatic production of hepcidin (Hepc), the iron hormone that modulates body

iron availability. Recent data suggest that Tfr2 is also expressed in extra hepatic tissues like bone

marrow. On the contrary, the widely expressed Tfr2 isoform is involved in iron export from

reticuloendotelial cells.

We have developed three targeted murine models. They are: (A)Tfr2 KO mice, where Tfr2 and

Tfr2 isoforms are germinally inactivated and in which a severe iron overload is present at adult

age; (B)Tfr2 KI mice, in which only the Tfr2isoform inactivated: animals have normal iron level

except in the spleen, where high iron deposition is observed; (C)Tfr2 LCKO-KI mice in which

Tfr2 is silenced only in the liver, while Tfr2 is silenced in all tissues: animals have a severe iron

overload at adult age (Roetto et al, 2010).

.

Tfr2 targeted animals are utilized to study:

Tfr2 isoforms involvement of in different stress conditions

-forced erythropoiesis

-hypoxia

-systemic inflammation

52

-cardiac ischemia and reperfusion (I/R) (in collaboration with Dr Penna/Prof Pagliaro, this

Department).

-iron depletion

Tfr2 involvement in erythropoiesis regulation (in collaboration with Dr Riondato, Dept of

Veterinary Sciences)

Tfr2 involvement in brain iron sensing (in collaboration with Dr Buffo, NICO Foundation)

Tfr2 beta isoform role in host-pathogen interaction (in collaboration with Prof Bozzaro, this

Department).

Part of the team is also involved in molecular biology diagnosis of main genetic iron disorders:

(Hemochromatosis, Iperferritinemia/Cataract and Haemoglobinopathies) (in collaboration with Dr

De Gobbi, this Department).

53


Nai A, Pellegrino RM, Rausa M, Pagani A, Boero M , Silvestri L, Saglio G, Roetto A,

Camaschella C. The erythroid function of transferrin receptor 2 revealed by Tmprss6

inactivation in different models of transferrin receptor 2 knockout mice. Haematologica. 2014

Jun;99(6):1016-21.

Roetto A, Porto G. A novel mutation in the CUB sequence of matriptase-2 (TMPRSS6) is

implicated in iron-resistant iron deficiency anaemia . Br J Haematol. 2013 Feb;160(4):566-7.

54

LIVER & GUT PATHOPHYSIOLOGY

Prof. Maurizio

PAROLA Full Professor

Prof. Antonella

MIGLIETTA Associate Professor

Dr. Erica

NOVO Assistant Professor

Dr. Claudia

BOCCA Assistant Professor

Dr. Claudia PATERNOSTRO Postdoctoral Fellowship

Dr. Stefania

CANNITO Postdoctoral

Fellowship

Dr. Elisabetta

MORELLO PhD Student


Main Research interests. To investigate (experimental and translational, at morphological, cell &

molecular biology level) mechanisms and processes underlying fibrogenic progression of chronic

liver diseases (CLDs) and the development of hepatocellular carcinoma (HCC) as well as of

colorectal cancer (CRC).

Specific areas of research.

To investigate the mechanistic role of hypoxia, hypoxia inducible factors (HIFs) and of related

target genes and mechanisms.

To investigate the molecular cross-talk between cell populations involved in the progression of

CLDs or in the development of HCC or CRC.

To investigate the role of epithelial-to-mesenchymal transition in cancer development.

To identify novel and reliable biomarkers and to test their diagnostic and/or prognostic significance

in human pathologic specimens (CLDs, HCC, CRC).

To investigate molecular mechanisms and significance of lipotoxicity in the progression of

NAFLD/NASH.

To investigate the effects of Rare Earth Elements (lanthanides) on human hepatocellular and

colorectal cancer cell growth and the underlying mechanisms.

55


Bocca C, Ievolella M, Autelli R, Motta M, Mosso L, Torchio B, Bozzo F, Cannito S,

Paternostro C, Colombatto S, Parola M, Miglietta A. Expression of COX-2 in human breast

cancer cells as a critical determinant of epithelial-mesenchymal transition and invasiveness.

Expert Opinion on Therapeutic Targets (2014),18:121-35.

Busletta C, Novo E, Parola M. Human induced pluripotent stem cells as a source of

hepatocyte-like cells: new kids on the block. Hepatology International (2013) 7:299-305.

Cannito S, Paternostro C, Busletta C, Bocca C, Colombatto S, Miglietta A, Novo E, Parola M.

Hypoxia, hypoxia inducible factors and fibrogenesis in chronic liver diseases. Histol

Histopathol (2014), 29:33-44.

Locatelli I, Sutti S, Jandal A, Vacchiano M, Bozzola C, Reutelingsperger C, Kusters D,

Benna S, Parola M, Paternostro C, Bugianesi E, McArthur S, Albano E, Perretti M. The

role of annexin A1 in modulating the progression of non-alcoholic steatohepatitis (NASH).

Hepatology (2014), 60:531-544.

Novo E, Cannito S, Paternostro C, Bocca C, Miglietta A. Parola M. Cellular and molecular

mechanisms in liver fibrogenesis. Arch Biochem Biophys (2014), 548:20-37.

Paternostro C, Busletta C, Cannito S, Novo E, Parola M. Hepatic angiogenesis and

fibrogenesis in the progression of chronic liver diseases. Current Angiogenesis (2013) 2:23-

29.

Povero D, Eguchi A, Niesman IR, Andronikou N, Mollerat du Jeu X, Mulya A, Berk M,

Lazic M, Thapaliya S, Parola M, Patel H, Feldstein AE. Hepatocytes release microparticles

during lipotoxicity that have potent pro-angiogenic effects in vitro and in vivo through Vanin-

1-dependent internalization. Science Signaling (2013) 6 (296) ra88.

Provenzano A, Milani S, Vizzutti F, Delogu W, Navari N, Novo E, Maggiora M, Maurino V.,

Laffi G, Parola M, Marra F. n-3 polyunsaturated fatty acids worsen inflammation and fibrosis

in experimental nonalcoholic steatohepatitis. Liver Int. (2014), 34:918-30.

Rovida E, Di Maira G, Tusa I, Cannito S, Paternostro C, Navari N, Vivoli E, Deng X, Gray

NS, Esparís-Ogando A, David E, Pandiella A, Dello Sbarba P, Parola M, Marra F. The

mitogen-activated protein kinase ERK5 regulates the development and growth of

hepatocellular carcinoma. Gut (2014), accepted on 4th August 2014.

Sansoè G, Aragno M, Mastrocola R, Paternostro C, Parola M. Calcium receptors located in

fibrotic septa: a new target to reduce portal pressure in liver cirrhosis. Clinical Science (2013),

125:67-75.

Villano G, Turato C, Quarta S, Ruvoletto M, Ciscato F, Terrin L, Semeraro R, Paternostro C,

Parola M, Alvaro D, Bernardi P, Gatta A, Pontisso P. Hepatic progenitor cells overexpress

SerpinB3.BMC Cell Biology (2014),15:5.

56

MOLECULAR ONCO-HEMATOLOGY

Dr. Alessandro

MOROTTI Assistant Professor

Dr. Cristina

PANUZZO Postdoctoral

Fellowship

Dr. Sabrina

CRIVELLARO PhD student

Dr. Giovanna

CARRÀ Fellowship


Definition of the role of PTEN, NFKBIA and DOK2 tumor suppressors in the pathogenesis of

Myeloproliferative Disorders

Identification of novel molecular targeted therapies for Myeloproliferative Disorders

57

Publications 2014

Morotti A, Panuzzo C, Crivellaro S, Carrà G, Guerrasio A, Saglio G. HAUSP

compartmentalization in chronic myeloid leukemia. Eur J Haematol. 2014 Aug 1. [Epub

ahead of print] PubMed PMID: 25082234.

Morotti A, Panuzzo C, Fava C, Saglio G. Kinase-inhibitor-insensitive cancer stem cells in

chronic myeloid leukemia. Expert Opin Biol Ther. 2014 Mar;14(3):287-99. Epub 2014 Jan 3.

PubMed PMID: 24387320.

Morotti A, Panuzzo C, Crivellaro S, Pergolizzi B, Familiari U, Berger AH, Saglio G, Pandolfi

PP. BCR-ABL disrupts PTEN nuclear-cytoplasmic shuttling through phosphorylation-

dependent activation of HAUSP. Leukemia. 2014 Jun;28(6):1326-33. Epub 2013 Dec 9.

PubMed PMID: 24317448.

58

MOLECULAR BIOLOGY

Prof. Michele

DE BORTOLI, Full Professor

Dr. Santina

CUTRUPI

Assistant Professor

Dr. Stefania

REINERI Postdoctoral

Fellowship

Dr. Laura

RICCI Postdoctoral

Fellowship

Dr. Andrea

IANELLO Fellowship

Dr. Valentina

MIANO PhD student

Dr. Giulio

FERRERO PhD Student

Ludovica

VANZAN Student


Our research focuses on the mechanisms of gene expression control in development and disease,

with special emphasis to the role of Estrogen Receptors. Stemming from fundamental aspect of

regulatory biology, we aim to identifying new therapeutic targets as well as clinically useful

biomarkers. Main pathways of our activity currently are:

- Genome-wide activity of unliganded ERα in breast cancer: transcriptome and chromatin level

studies in breast cancer cells with specific deletion of ERα unravel an unespected role of this

receptor in the absence of hormones. This data has implications in the problem of resistance to

breast cancer treatment with Aromatase Inhibitors.

- the role of LncRNAs in regulatory networks of gene regulation and phenotype determination by

controlling transcription, chromatin epigenetic regulation and post-transcriptional events in breast

cancer. A subset of these ERα-dependent lncRNAs have important functions in the regulation of a

hormone-dependent, epithelial phenotype in breast cancer and are proposed as innovative markers

of hormone-responsiveness.

- the role of ERα in the control of Neuroglobin transcription in neuronal models. This is afforded by

identifying the regulatory sequences in the neuroglobin locus and chromatin-level studies following

59

estrogen stimulation. an important application is the recognized action of estrogens in

neuroprotection

- the role of ERα in the immunomodulation of Multiple Sclerosis (MS) disease during pregnancy

and physical activity programs for MS patients.

New projects:

- Epigenetic modifications of stem cells maintained in culture (Mareschi, Canepa)

- Epigenetic modifications of T lymphocytes infiltrated in pancreas cancer (Novelli)

60


Caizzi L, Ferrero G, Cutrupi S, Cordero F, Ballaré C, Miano V, Reineri S, Ricci L, Friard O,

Testori A, Cora D, Caselle M, Di Croce L, De Bortoli M. Genome-wide activity of

unliganded estrogen receptor-α in breast cancer cells. PNAS, 2014, 111(13):4892-7.

Cutrupi S, Ferrero G, Reineri S, Cordero F, De Bortoli M. Genomic lens on neuroglobin

transcription. IUBMB Life. 2014 Jan;66(1):46-5.

Cimino D, De Pittà C, Orso F, Zampini M, Casara S, Penna E, Quaglino E, Forni M,

Damasco C, Pinatel E, Ponzone R, Romualdi C, Brisken C, De Bortoli M, Biglia N, Provero

P, Lanfranchi G, Taverna D. miR148b is a major coordinator of breast cancer progression in a

relapse-associated microRNA signature by targeting ITGA5, ROCK1, PIK3CA, NRAS, and

CSF1. FASEB J 2013; 27: 1223-35.

Cutrupi S, Ferrero G, Reineri S, Cordero F, De Bortoli M. Genomic lens on neuroglobin

transcription. IUBMB Life. 2014 ;66(1):46-51.

61

MOLECULAR VIROLOGY AND ANTIVIRAL RESEARCH

Prof. David

LEMBO Associate Professor

Dr. Manuela

DONALISIO Assistant Professor

Dr. Andrea

CIVRA PhD Student

Dr. Valeria

CAGNO PhD Student


• Studies on virus-cell interactions

• Research and development of antiviral molecules

• Research and development of innovative formulations based on nanocarriers for the delivery

of antiviral drugs, peptides, and nucleic acids

Viruses routinely screened for antiviral drug discovery in the lab:

- Herpes simplex virus 1 and 2

- Cytomegalovirus

- Human papillomaviruses

- Rotaviruses

- Respiratory syncytial virus

- Rhinovirus

- Adenovirus

62

Recent Publications (2013/2014) Donalisio M, Ranucci E, Cagno V, Civra A, Manfredi A, Cavalli R, Ferruti P, Lembo D.

Agmatine-containing poly(amidoamine)s as novel class of antiviral macromolecules:

structural properties and in vitro evaluation of infectivity inhibition. Antimicrob Agents

Chemother. 2014 Aug 4. pii: AAC.03420-14. [Epub ahead of print]

Cagno V, Donalisio M, Civra A, Volante M, Veccelli E, Oreste P, Rusnati M, Lembo D.

Highly Sulfated K5 Escherichia coli Polysaccharide Derivatives Inhibit Respiratory Syncytial

Virus Infectivity in Cell Lines and Human Tracheal-Bronchial Histocultures. Antimicrob

Agents Chemother. 2014 Aug;58(8):4782-94.

Donalisio M, Massari S, Argenziano M, Manfroni G, Cagno V, Civra A, Sabatini S, Cecchetti

V, Loregian A, Cavalli R, Lembo D, Tabarrini O. Ethyl 1,8-Naphthyridone-3-carboxylates

Downregulate Human Papillomavirus-16 E6 and E7 Oncogene Expression. J Med Chem.

2014 Jul 10;57(13):5649-63.

Calcio Gaudino E, Tagliapietra S, Martina K, Barge A, Lolli M, Terreno E, Lembo D,

Cravotto G. A novel SWCNT platform bearing DOTA and β-cyclodextrin units. "One shot"

multidecoration under microwave irradiation. Org Biomol Chem. 2014 Jul 14;12(26):4708-15.

Lembo D, Donalisio M, Laine C, Cagno V, Civra A, Bianchini EP, Zeghbib N, Bouchemal K.

Auto-associative heparin nanoassemblies: A biomimetic platform against the heparan sulfate-

dependent viruses HSV-1, HSV-2, HPV-16 and RSV. Eur J Pharm Biopharm. 2014 May 14.

pii: S0939-6411(14)00156-8. [Epub ahead of print]

Russo E, Gaglianone N, Baldassari S, Parodi B, Cafaggi S, Zibana C, Donalisio M, Cagno V,

Lembo D, Caviglioli G. Preparation, characterization and in vitro antiviral activity evaluation

of foscarnet-chitosan nanoparticles. Colloids Surf B Biointerfaces. 2014 Jun 1;118:117-25.

Donalisio M, Cagno V, Vallino M, Moro GE, Arslanoglu S, Tonetto P, Bertino E, Lembo D.

Inactivation of high-risk human papillomaviruses by Holder pasteurization: implications for

donor human milk banking. J Perinat Med. 2014 Jan;42(1):1-8.

Donalisio M, Nana HM, Ngane RA, Gatsing D, Tchinda AT, Rovito R, Cagno V, Cagliero C,

Boyom FF, Rubiolo P, Bicchi C, Lembo D. In vitro anti-Herpes simplex virus activity of

crude extract of the roots of Nauclea latifolia Smith (Rubiaceae). BMC Complement Altern

Med. 2013 Oct 16;13:266.

Bon I, Lembo D, Rusnati M, Clò A, Morini S, Miserocchi A, Bugatti A, Grigolon S,

Musumeci G, Landolfo S, Re MC, Gibellini D. Peptide-derivatized SB105-A10 dendrimer

inhibits the infectivity of R5 and X4 HIV-1 strains in primary PBMCs and cervicovaginal

histocultures. PLoS One. 2013 Oct 7;8(10):e76482.

Cavalli R, Bisazza A, Lembo D. Micro- and nanobubbles: a versatile non-viral platform for

gene delivery. Int J Pharm. 2013 Nov 18;456(2):437-45. Epub 2013 Sep 2.

Salvador B, Sexton NR, Carrion R Jr, Nunneley J, Patterson JL, Steffen I, Lu K, Muench MO,

Lembo D, Simmons G. Filoviruses utilize glycosaminoglycans for their attachment to target

cells. J Virol. 2013 Mar;87(6):3295-304.

63

Lembo D, Swaminathan S, Donalisio M, Civra A, Pastero L, Aquilano D, Vavia P, Trotta F,

Cavalli R. Encapsulation of Acyclovir in new carboxylated cyclodextrin-based nanosponges

improves the agent's antiviral efficacy. Int J Pharm. 2013 Feb 25;443(1-2):262-72.

64

Research Team

www.unito.it/dscb

NEPHROLOGY AND CLINICAL NUTRITION

Prof. Paolo AVAGNINA Associate Professor

Dr. Giorgina B. PICCOLI Assistant Professor

Dr. Federica N. VIGOTTI Postdoctoral Fellowship

Dr. Martina FERRARESI Specialization Student

Dr. Irene CAPIZZI Fellowship

Dr. Elena MONGILARDI Fellowship

Current Research Projects 1) PROTEREN: a new qualitative, multiple-choice approach to low protein diets (LPDs) in chronic kidney disease (CKD).

2) RENIRE: to protect the kidney by losing weight. A new dietary approach for Chronic Kidney Disease overweight/obese patients.

3) Development of a clinical flowchart for end stage CKD patients in conservative therapy, to help defining the “ideal” moment for the start of renal replacement therapy.

4) Quality of life of patients on LPDs: a multicentric survey (Torino-Cagliari-Pisa-Solofra). Expected involvement 500 patients

5) POLIFERE: somatostatin analogue therapy for hepatorenal policystosis.

6) Participation to the european study EQUAL on CKD patients older than 65 years old (first Italian centre for enrolment rate)

7) Outcome research on pregnancy and CKD (cooperation with the Italian Study Group for Kidney and Pregnancy).

8) Ecodialysis: waste management in dialysis. An economical and ecological sustainability project (with Politecnico di Torino)

65

Research products

www.unito.it/dscb


Piccoli GB, Giuva D, Vigotti FN, Ruff D, Finnel S, Seman A, Demartino F, Picillo R, Richiero G. Four seasons for reflecting. Summer: taste and touch. Nephrol Dial Transplant. 2014 Sep;29(9):1675-9.

Piccoli GB, Deagostini MC, Vigotti FN, Ferraresi M, Moro I, Consiglio V, Scognamiglio S, Mongilardi E, Clari R, Aroasio E, Versino E, Porpiglia F. Which low-protein diet for which CKD patient? An observational, personalized approach. Nutrition. 2014 Sep;30(9):992-9.

Bellizzi V, Chiodini P, Cupisti A, Viola BF, Pezzotta M, De Nicola L, Minutolo R, Barsotti G, Piccoli GB, Di Iorio B. Very low-protein diet plus ketoacids in chronic kidney disease and risk of death during end-stage renal disease: an historical, cohort, controlled study. Nephrol Dial Transplant. 2014 Jul 30. pii: gfu251. [Epub ahead of print] PubMed PMID: 25082793.

Piccoli GB, Roggero S, De Pascale A, Vigotti FN, Piga A, Veltri A. The Case | Microhematuria in a patient with beta-thalassemia major: a casual or a causal association? Kidney Int. 2014 Aug;86(2):450-2.

Piccoli GB, Guzzo G, Vigotti FN, Scognamiglio S, Consiglio V, Aroasio E, Gonella S, Castelluccia N, Mauro G, Colombi N. Chronic dialysis discontinuation: a systematic narrative review of the literature in the new millennium. Int J Artif Organs. 2014 Jul 31;37(7):556-62.

Piccoli GB, Guzzo G, Vigotti FN, Capizzi I, Clari R, Scognamiglio S, Consiglio V, Aroasio E, Gonella S, Veltri A, Avagnina P. Tailoring dialysis and resuming low-protein diets may favor chronic dialysis discontinuation: report on three cases. Hemodial Int. 2014 Jul;18(3):590-5.

Piccoli GB, Priola AM, Vigotti FN, Guzzo G, Veltri A. Renal infarction versus pyelonephritis in a woman presenting with Fever and flank pain. Am J Kidney Dis. 2014 Aug;64(2):311-4. Epub 2014 Apr 22.

Piccoli GB, Cabiddu G, Daidone G, Guzzo G, Maxia S, Ciniglio I, Postorino V, Loi V, Ghiotto S, Nichelatti M, Attini R, Coscia A, Postorino M, Pani A; Italian Study Group “Kidney and Pregnancy”. The children of dialysis: live-born babies from on-dialysis mothers in Italy-an epidemiological perspective comparing dialysis, kidney transplantation and the overall population. Nephrol Dial Transplant. 2014 Aug;29(8):1578-86.

Piccoli GB. [Spending review, personal view, water and waste in (home) hemodialysis]. G Ital Nefrol. 2014 Jan-Feb;31(1). pii: gin/31.1.3. Italian. PubMed PMID: 24671836.

Piccoli GB, Leone F, Attini R, Parisi S, Fassio F, Deagostini MC, FerraresiM, Clari R, Ghiotto S, Biolcati M, Giuffrida D, Rolfo A, Todros T. Association of low-protein supplemented diets with fetal growth in pregnant women with CKD. Clin J Am Soc Nephrol. 2014 May;9(5):864-73.

Veltri A, Gazzera C, Busso M, Solitro F, Piccoli GB, Andreetto B, Garetto I. T1a as the Sole Selection Criterion for RFA of Renal Masses: Randomized Controlled Trials versus Surgery Should Not Be Postponed. Cardiovasc Intervent Radiol. 2013 Dec 13.

66

Research products

www.unito.it/dscb

Pecci A, Klersy C, Gresele P, Lee KJ, De Rocco D, Bozzi V, Russo G, Heller PG, Loffredo G, Ballmaier M, Fabris F, Beggiato E, Kahr WH, Pujol-Moix N, Platokouki H, Van Geet C, Noris P, Yerram P, Hermans C, Gerber B, Economou M, De Groot M, Zieger B, De Candia E, Fraticelli V, Kersseboom R, Piccoli GB, Zimmermann S, Fierro T, Glembotsky AC, Vianello F, Zaninetti C, Nicchia E, Güthner C, Baronci C, Seri M, Knight PJ, Balduini CL, Savoia A. MYH9-related disease: a novel prognostic model to predict the clinical evolution of the disease based on genotype-phenotype correlations. Hum Mutat. 2014 Feb;35(2):236-47.

Piccoli GB, Tavassoli E, Melluzza C, Grassi G, Monzeglio C, Donvito V, Leone F, Attini R, Ghiotto S, Clari R, Moro I, Fassio F, Parisi S, Pilloni E, Vigotti FN, Giuffrida D, Rolfo A, Todros T. Severe diabetic nephropathy in type 1 diabetes and pregnancy--a case series. Rev Diabet Stud. 2013 Spring;10(1):68-78.

Piccoli GB, Clari R, Ghiotto S, Castelluccia N, Colombi N, Mauro G, Tavassoli E, Melluzza C, Cabiddu G, Gernone G, Mongilardi E, Ferraresi M, Rolfo A, Todros T. Type 1 diabetes, diabetic nephropathy, and pregnancy: a systematic review and meta-study. Rev Diabet Stud. 2013 Spring;10(1):6-26.

Piccoli GB, Ferraresi M, Deagostini MC, Vigotti FN, Consiglio V, Scognamiglio S, Moro I, Clari R, Fassio F, Biolcati M, Porpiglia F. Vegetarian low-protein diets supplemented with keto analogues: a niche for the few or an option for many? Nephrol Dial Transplant. 2013 Sep;28(9):2295-305.

De Pascale A, Piccoli GB, Priola SM, Rognone D, Consiglio V, Garetto I, Rizzo L, Veltri A. Diffusion-weighted magnetic resonance imaging: new perspectives in the diagnostic pathway of non-complicated acute pyelonephritis. Eur Radiol. 2013 Nov;23(11):3077-86.

Piccoli GB, Attini R, Parisi S, Vigotti FN, Daidola G, Deagostini MC, Ferraresi M, De Pascale A, Porpiglia F, Veltri A, Todros T. Excessive urinary tract dilatation and proteinuria in pregnancy: a common and overlooked association? BMC Nephrol. 2013 Feb 27;14:52.

Piccoli GB, Daidola G, Attini R, Parisi S, Fassio F, Naretto C, Deagostini MC, Castelluccia N, Ferraresi M, Roccatello D, Todros T. Kidney biopsy in pregnancy: evidence for counselling? A systematic narrative review. BJOG. 2013 Mar;120(4):412-27.

Piccoli GB, Gaglioti P, Attini R, Parisi S, Bossotti C, Olearo E, Oberto M, Ferraresi M, Rolfo A, Versino E, Biolcati M, Todros T. Pre-eclampsia or chronic kidney disease? The flow hypothesis. Nephrol Dial Transplant. 2013 May;28(5):1199-206.

Piccoli GB, Arduino S, Attini R, Parisi S, Fassio F, Biolcati M, Pagano A, Bossotti C, Vasario E, Borgarello V, Daidola G, Ferraresi M, Gaglioti P, Todros T. Multiple pregnancies in CKD patients: an explosive mix. Clin J Am Soc Nephrol. 2013 Jan;8(1):41-50.

Rolfo A, Attini R, Nuzzo AM, Piazzese A, Parisi S, Ferraresi M, Todros T, Piccoli GB. Chronic kidney disease may be differentially diagnosed from preeclampsia by serum biomarkers. Kidney Int. 2013 Jan;83(1):177-81.

Giacchino F, Piccoli G, Colla L, Fenoglio R, Marazzi F, Amore A, Rollino C, Stratta P. [Acute pielonephritis and renal abscesses in Piedmont and Aosta Valley]. G Ital Nefrol. 2014 Jul-Aug;31(4). pii: gin/31.4.10. Italian. PubMed PMID: 25098465.

67

Research products

www.unito.it/dscb

Piccoli G. [The Canadian Society of Nephrology 2014 clinical practice guideline for timing the initiation of chronic dialysis: a paradigm shift and the return of the clinical nephrologist]. G Ital Nefrol. 2014 May-Jun;31(3). Pii: gin/31.3.8. Italian. PubMed PMID: 25030011.

Vigotti FN, Guzzo G, Capizzi I, Teta L, Ippolito D, Mirasole S, Giuffrida D, Avagnina P, Piccoli G. [Intensive weight-loss in dialysis: a personalized approach]. G Ital Nefrol. 2014 Mar-Apr;31(2).

Capobianco M, Piccoli G, Neve Vigotti F, Scapoli P, Deagostini MC, Albera C, Roccatello D, Bertolotto A. Interferon beta-related nephropathy and interstitial lung disease: a new association and a long-term warning. Mult Scler. 2014 Jan 20.

Piccoli Giorgina (indexed as Barbara PG). “Advice to the medical students in my service": the rediscovery of a golden book by Jean Hamburger, father of nephrology and of medical humanities. Philos Ethics Humanit Med. 2013 Mar 15;8:2.

68

NEUROLOGY

Prof. Luca

DURELLI Full Professor

Dr. Marinella

CLERICO Assistant Professor

Dr. Stefania

DE MERCANTI PhD Student


We are conducting different projects on Multiple Sclerosis, the more relevant are 1) Role of

estrogen receptor alpha in multiple sclerosis 2) Phase IV study on comparison of the efficacy of

Natalizumab vs Fingolimod in Multiple Sclerosis 3) Role of anti-IFN neutralizing antibodies: a

Cochrane review 4) Study of psychiatric disorder in Multiple Sclerosis: a longitudinal study 5)

Psycho-pedagogic project on multiple sclerosis patients.

In the near future we will start some researches on different neurologic disease: Neurodegenerative

disorders (Parkinson disease and Alzheimer disease), Hereditary ataxias.

69


Patti F, Morra VB, Amato MP, Trojano M, Bastianello S, Tola MR, Durelli L, Cottone

S,Plant A, Picconi O; COGIMUS Study Group. Subcutaneous interferon β-1a may protect

against cognitive impairment in patients with relapsing-remitting multiple sclerosis: 5-year

follow-up of the COGIMUS study. PLoS One. 2013 Aug30;8(8):e74111.

Pupillo E, Messina P, Giussani G, Logroscino G, Zoccolella S, Chiò A, Calvo A, Corbo M,

Lunetta C, Durelli L, Marin B, Mitchell D, Hardiman O, Rooney J, Stevic Z, Bandettini di

Poggio M, Filosto M, Cotelli MS, Perini M, Riva N, Tremolizzo L, Vitelli E, Damiani D,

Beghi E; EURALS Consortium. Physical activity and amyotrophic lateral sclerosis: a

European population-based case-control study. Ann Neurol. 2014 May;75(5):708-16.

Chiò A, Calvo A, Bovio G, Canosa A, Bertuzzo D, Galmozzi F, Cugnasco P, Clerico M, De

Mercanti S, Bersano E, Cammarosano S, Ilardi A, Manera U, Moglia C, Sideri R, Marinou K,

Bottacchi E, Pisano F, Cantello R, Mazzini L, Mora G; for the Piemonte and Valle d’Aosta

Register for Amyotrophic Lateral Sclerosis (PARALS). Amyotrophic Lateral Sclerosis

Outcome Measures and the Role of Albumin and Creatinine: A Population-Based Study.

JAMA Neurol. 2014 Jul 21.

Clerico M, Schiavetti I, De Mercanti SF, Piazza F, Gned D, Brescia Morra V, Lanzillo R,

Ghezzi A, Bianchi A, Salemi G, Realmuto S, Sola P, Vitetta F, Cavalla P, Paolicelli D,

Trojano M, Sormani MP, Durelli L. Treatment of Relapsing-Remitting Multiple Sclerosis

After 24 Doses of Natalizumab: Evidence From an Italian Spontaneous, Prospective, and

Observational Study (the TY-STOP Study). JAMA Neurol. 2014 Aug 1;71(8):954-60.

Rolla S, Bardina V, De Mercanti S, Quaglino P, Palma RD, Gned D, Brusa D, Durelli L,

Novelli F, Clerico M. Th22 cells are expanded in multiple sclerosis and are resistant to IFN-β.

J Leukoc Biol. 2014 Aug 5.

Soragni E, Miao W, Iudicello M, Jacoby D, Demercanti S, Clerico M, Longo F, Piga A, Ku S,

Campau E, Du J, Penalver P, Rai M, Madara JC, Nazor K, O'Connor M, Maximov A, Loring

JF, Pandolfo M, Durelli L, Gottesfeld JM, Rusche JR. Epigenetic therapy for Friedreich's

ataxia. Ann Neurol. 2014 Aug 27.

70

OBSTRUCTIVE LUNG DISEASE

Prof. Fabio L.M.

RICCIARDOLO Assistant Professor

Dr. Valentina

SORBELLO Postdoctoral

Fellowship

Dr. Ilaria

LEONE Fellowship


The current research projects are focused on three main directions:

clinical research about asthma control in relation to functional parameters, non-invasive

biomarkers and comordibities;

translational research in Asthma and COPD (Chronic Obstructive Pulmonary Disease) focused on

the role of invasive biomarkers involved in pathogenetic mechanisms related to disease severity;

basic research focused on the role of epithelial cells and fibroblasts in the modulation of airway

chronic inflammation and remodeling.

71


Ricciardolo FLM, Sorbello V, Ciprandi G. FeNO as biomarker for asthma phenotyping and

management. Allergy Asthma Proc 2014; In Press.

Ciprandi G, Ricciardolo FLM, Sciavetti I, Cirillo I. Allergic rhinitis phenotypes based on

bronchial hyperreactivity to methacholine. Am J Rhinol Allergy 2014; In Press.

Ciprandi G, Cirillo I, Ricciardolo FLM. The relevance of obesity on asthma control in

identical twins. Allergology Intern 2014; In Press.

Papi A, Blasi F, Canonica GW, Cazzola M, Centanni S, Foschino Barbaro MP, Melani AS,

Paggiaro P, Ricciardolo F, Rossi A, Scichilone N. Fluticasone propionate/formoterol: A

fixed-combination therapy with flexible dosage. Eur J Intern Med. 2014 Oct;25(8):695-700.

Epub 2014 Jul 19. Review.

Ciprandi G, Schiavetti I, Bellezza Fontana R, Sorbello V, Ricciardolo FLM. Overweight and

obesity as risk factors for impaired lung function in patients with asthma: A real-life

experience. Allergy Asthma Proc. 2014 Jul-Aug;35(4):e62-71.

Masoero M, Bellocchia M, Ciuffreda A, Ricciardolo FLM, Rolla G, Bucca C. Laryngeal

spasm mimicking asthma and vitamin d deficiency. Allergy Asthma Immunol Res. 2014

May;6(3):267-9. Epub 2014

Malerba M, Radaeli A, Olivini A, Damiani G, Ragnoli B, Montuschi P, Ricciardolo FLM.

Exhaled nitric oxide as a biomarker in COPD and related comorbidities. Biomed Res Int.

2014;2014:271918. Epub 2014 Feb 27.

Eleuteri E, Di Stefano A, Vallese D, Gnemmi I, Pitruzzella A, Tarro Genta F, Delle Donne

L, Cappello F, Ricciardolo FL, Giannuzzi P. Fibrosis markers and CRIM1 increase in

chronic heart failure of increasing severity. Biomarkers. 2014 May;19(3):214-21. Epub 2014

Mar 11.

Boita M, Garzaro M, Raimondo L, Riva G, Mazibrada J, Pecorari G, Bucca C, Bellone G,

Vizio B, Heffler E, Ricciardolo FLM, Rolla G. Eosinophilic inflammation of chronic

rhinosinusitis with nasal polyps is related to OX40 ligand expression. Boita M, Garzaro M,

Raimondo L, Riva G, Mazibrada J, Pecorari G, Bucca C, Bellone G, Vizio B, Heffler E,

Ricciardolo FLM, Rolla G. Innate Immun. 2014 Feb 28. [Epub ahead of print]

Ricciardolo FLM. Revisiting the role of exhaled nitric oxide in asthma. Curr Opin Pulm

Med. 2014 Jan;20(1):53-9. Review.

Ciprandi G, Ricciardolo FL, Signori A, Schiavetti I, Monardo M, Ferraro MR, Cirillo I.

Increased body mass index and bronchial impairment in allergic rhinitis. Am J Rhinol

Allergy. 2013 Nov-Dec;27(6):e195-201.

Ciprandi G, Cirillo I, Pasotti F, Ricciardolo FLM. FEF25-75: a marker for small airways and

asthma control. Ann Allergy Asthma Immunol. 2013 Sep;111(3):233. Epub 2013 Jul 16. No

abstract available.

72

http://www.ncbi.nlm.nih.gov/pubmed/25051902
















Ricciardolo FLM, Sabatini F, Sorbello V, Benedetto S, Defilippi I, Petecchia L, Usai C,

Gnemmi I, Balbi B, De Rose V, Ten Hacken NH, Postma DS, Timens W, Di Stefano A.

Expression of vascular remodelling markers in relation to bradykinin receptors in asthma

and COPD. Thorax. 2013 Sep;68(9):803-11. Epub 2013 Jun 5.

Heffler E, Pizzimenti S, Badiu I, Guida G, Ricciardolo FLM, Bucca C, Rolla G. Nasal nitric

oxide is a marker of poor asthma control. J Breath Res. 2013 Jun;7(2):026009. Epub 2013

May 10.

Sabatini F, Luppi F, Petecchia L, Stefano AD, Longo AM, Eva A, Vanni C, Hiemstra PS,

Sterk PJ, Sorbello V, Fabbri LM, Rossi GA, Ricciardolo FLM. Bradykinin-induced

asthmatic fibroblast/myofibroblast activities via bradykinin B2 receptor and different

MAPK pathways. Eur J Pharmacol. 2013 Jun 15;710(1-3):100-9. Epub 2013 Apr 12.

Albano GD, Di Sano C, Bonanno A, Riccobono L, Gagliardo R, Chanez P, Gjomarkaj M,

Montalbano AM, Anzalone G, La Grutta S, Ricciardolo FLM, Profita M. Th17 immunity in

children with allergic asthma and rhinitis: a pharmacological approach. PLoS One.

2013;8(4):e58892. Epub 2013 Apr 3.

Ricciardolo FLM, Sorbello V, Silvestri M, Giacomelli M, Debenedetti VM, Malerba M,

Ciprandi G, Rossi GA, Rossi A, Bontempelli M. TNF-alpha, IL-4R-alpha and IL-4

polymorphisms in mild to severe asthma from Italian Caucasians. Int J Immunopathol

Pharmacol. 2013 Jan-Mar;26(1):75-84.

Sterk PJ, Ricciardolo FLM. Clinical-biological phenotyping beyond inflammation in asthma

delivers. Am J Respir Crit Care Med. 2013 Jan 15;187(2):117-8. No abstract available.

Cirillo I, Ricciardolo FLM, Medusei G, Signori A, Ciprandi G. Exhaled nitric oxide may

predict bronchial hyperreactivity in patients with allergic rhinitis. Int Arch Allergy Immunol.

2013;160(3):322-8. Epub 2012 Oct 9.

Overbeek SA, Kleinjan M, Henricks PA, Kamp VM, Ricciardolo FLM, Georgiou NA,

Garssen J, Kraneveld AD, Folkerts G. Chemo-attractant N-acetyl proline-glycine-proline

induces CD11b/CD18-dependent neutrophil adhesion. Biochim Biophys Acta. 2013

Jan;1830(1):2188-93. Epub 2012 Oct 3.

73


















PEDIATRICS

Prof. Antonio G.

PIGA Associate Professor

Dr. Filomena

LONGO Postdoctoral

Fellowship

Dr. Simona

ROGGERO Postdoctoral

Fellowship

Dr. Arianna

CANUTO Fellowship

Dr. Erica

LONGONI Fellowship

Dr. Marianna

GENISIO Fellowship

Dr. Ilaria

ALASIA Fellowship

Dr. Cecilia

Maria PINI Fellowship


-Modulation of erythropoiesis by activin receptor ligands

-Pathogen inactivation in blood products

-New markers of iron toxicity in iron disorders

-Optimization of iron chelation therapy by combination of iron chelating agents

-New approaches to transition medicine for hemoglobinopathies and rare diseases

74


Piga A, Longo F, Origa R, Roggero S, Pinna F, Zappu A, Castiglioni C, Cappellini MD.

Deferasirox for cardiac siderosis in β-thalassaemia major: a multicentre, open label,

prospective study. Br J Haematol. 2014 Jun 30. [Epub ahead of print]

Pennell DJ, Porter JB, Piga A, Lai Y, El-Beshlawy A, Belhoul KM, Elalfy M, Yesilipek A,

Kilinç Y, Lawniczek T, Habr D, Weisskopf M, Zhang Y, Aydinok Y; CORDELIA study

investigators. A 1-year randomized controlled trial of deferasirox vs deferoxamine for

myocardial iron removal in β-thalassemia major (CORDELIA). Blood. 2014 Mar

6;123(10):1447-54.

Derchi G, Galanello R, Bina P, Cappellini MD, Piga A, Lai ME, Quarta A, Casu G, Perrotta

S, Pinto V, Musallam KM, Forni GL; Webthal Pulmonary Arterial Hypertension Group.

Prevalence and risk factors for pulmonary arterial hypertension in a large group of β-

thalassemia patients using right heart catheterization: a Webthal study. Circulation. 2014 Jan

21;129(3):338-45.

Soragni E, Miao W, Iudicello M, Jacoby D, De Mercanti S, Clerico M, Longo F, Piga A, Ku

S, Campau E, Du J, Penalver P, Rai M, Madara JC, Nazor K, O'Connor M, Maximov A,

Loring JF, Pandolfo M, Durelli L, Gottesfeld JM, Rusche JR. Epigenetic therapy for

Friedreich ataxia. Ann Neurol. 2014 Aug 27.

Piccoli GB, Roggero S, De Pascale A, Vigotti FN, Piga A, Veltri A. The case.

Microhematuria in a patient with beta- thalassemia major: a casual or a causal association?

Kidney Int. 2014 Aug;86(2):450-2.

Borgna-Pignatti C, Garani MC, Forni GL, Cappellini MD, Cassinerio E, Fidone C, Spadola

V, Maggio A, Restivo Pantalone G, Piga A, Longo F, Gamberini MR, Ricchi P, Costantini S,

D'Ascola D, Cianciulli P, Lai ME, Carta MP, Ciancio A, Cavalli P, Putti MC, Barella S,

Amendola G, Campisi S, Capra M, Caruso V, Colletta G, Volpato S. Hepatocellular

carcinoma in thalassaemia: an update of the Italian Registry. Br J Haematol. 2014

Oct;167(1):121-6.

Roggero S, Vullo S, Volpe G, Piga A, Albera C. Management of pulmonary arterial

hypertension associated to thalassemia: when pulmonary endarterectomy is the best

therapeutical option? A case report. J Thromb Thrombolysis. 2014 May 1.

Ruo Redda MG, Allis S, Reali A, Bartoncini S, Roggero S, Anglesio SM, Piga A. Complete

recovery from paraparesis in spinal cord compression due to extramedullary haemopoiesis in

beta-thalassaemia by emergency radiation therapy. Intern Med J. 2014 Apr;44(4):409-12.

Origa R, Sollaino MC, Borgna-Pignatti C, Piga A, Feliu Torres A, Masile V, Galanello R. α-

globin gene quadruplication and heterozygous β-thalassemia: a not so rare cause of

thalassemia intermedia. Acta Haematol. 2014;131(3):162-4.

Fernandes JL, Sampaio EF, Fertrin K, Coelho OR, Loggetto S, Piga A, Verissimo M, Saad

ST. Amlodipine reduces cardiac iron overload in patients with thalassemia major: a pilot trial.

Am J Med. 2013 Sep;126(9):834-7.

Pennell DJ, Udelson JE, Arai AE, Bozkurt B, Cohen AR, Galanello R, Hoffman TM, Kiernan

MS, Lerakis S, Piga A, Porter JB, Walker JM, Wood J; American Heart Association

Committee on Heart Failure and Transplantation of the Council on Clinical Cardiology and

75

Council on Cardiovascular Radiology and Imaging. Cardiovascular function and treatment in

β-thalassemia major: a consensus statement from the American Heart Association.

Circulation. 2013 Jul 16;128(3):281-308.

Piga A, Longo F, Musallam KM, Cappellini MD, Forni GL, Quarta G, Chiavilli F,

Commendatore F, Mulas S, Caruso V, Galanello R. Assessment and management of iron

overload in β-thalassaemia major patients during the 21st century: a real-life experience from

the Italian WEBTHAL project. Br J Haematol. 2013 Jun;161(6):872-83.

76

PHARMACOLOGY AND CLINICAL PHARMACOLOGY SERVICE

Prof. Silvia

RACCA Associate Professor

Dr. Barbara

MOGNETTI Assistant Professor

Dr. Giuliana

ABBADESSA Research Technician

Dr. Elisa

PIRRO Research Technician

Dr. Silvia

DE FRANCIA Pharmacology

Consultant

Dr. Vitina

CARRIERO Postdoctoral

Fellowship

Dr. Francesca

Bertolini PhD Student

Dr. Paolo

CARDALANA Fellowship


• Retrospective (and prospective) pharmacogenetic studies on potential impact of genetic

variants on drug levels (gene under investigation: CYP2B6 for Mitotane and Phenytoin; CYP1A1

and CYP1A2 for Deferasirox; CYP2C19 for Voriconazole, ABCB1 for Dasatinib and Phenytoin).

• The importance of BCLI and N363S polymorphisms on the response to glucocorticoids in

asthma treatment.

• Study on the antioxidant activity of anabolic androgen steroids in heart cell culture.

• Biomelecular and genetic investigation to ascertain suspected link between

lymphangioleiomyomatosis and angiomyolipoma.

• Study of the anti-diabetic drug metformin influence on rat skeletal muscle to establish if

metformin is able to improve muscle mass and physical performance.

Clinical Pharmacology service activities

Plasma analyses for Therapeutic Drug Monitoring of: antineoplastic agents (Mitotane,

Methotrexate); tyrosine kinases inhibitors (Imatinib, Dasatinib and Nilotinib); cardiovascular drugs

(Hydroquinidine, Flecainide, Amiodarone and Propafenone); antifungal agents (Posaconazole and

Voriconazole); chelating agents (Deferasirox); antiepileptics drugs (Levetiracetam, Oxcarbazepine

and Metabolite, Lamotrigine e Phenytoin); antibiotics (Vancomycin, Teicoplanin).

77


Dimauro I, Grasso L, Fittipaldi S, Fantini C, Mercatelli N, Racca S, Geuna S, Di

Gianfrancesco A, Caporossi D, Pigozzi F, Borrione P. Platelet-rich plasma and skeletal

muscle healing: a molecular analysis of the early phases of the regeneration process in an

experimental animal model. PLoS One. 2014; 9(7):e102993.

Trotta F, Dianzani C, Caldera F, Mognetti B, Cavalli R. The application of nanosponges to

cancer drug delivery. Expert Opin Drug Deliv. 2014 Jun;11(6):931-41.

Mognetti B, La Montagna G, Perrelli MG, Marino S, Pagliaro P, Cracco CM, Penna C.

Zoledronic acid and leuprorelin acetate, alone or in combination, similarly reduce

proliferation and migration of prostate cancer cells In vitro. International Journal of Medical

Biology, 2014, 1, ID 235870

Cusato J, Allegra S, Massano D, De Francia S, Piga A, D'Avolio A. Influence of single-

nucleotide polymorphisms on deferasirox C(trough) levels and effectiveness.

Pharmacogenomics J., 2014.

Borrione P, Grasso L, Chierto E, Geuna S, Racca S, Abbadessa G, Ronchi G, Faiola F,

Di Gianfrancesco A, Pigozzi F. Experimental model for the study of the effects of platelet-

rich plasma on the early phases of muscle healing. BloodTransfus. 2014;12 Suppl 1:s221-8.

De Francia S, DʼAvolio A, Ariaudo A, Pirro E, Piccione F, Simiele M, Fava C, Calcagno

A, Di Perri G, Saglio G. Plasma and intracellular imatinib concentrations in patients with

chronic myeloid leukemia. Ther Drug Monit. 2014;36(3):410-2.

Terzolo M, Zaggia B, Allasino B, De Francia S. Practical treatment using mitotane for

adrenocortical carcinoma. Curr Opin Endocrinol Diabetes Obes. 2014; 21(3):159-65.

Mognetti B, La Montagna G, Perrelli MG, Pagliaro P, Penna C. Bone marrow

mesenchymal stem cells increase motility of prostate cancer cells via production of stromal

cell-derived factor-1α. J Cell Mol Med., 2013 Feb;17(2):287-92.

Terzolo M, Baudin AE, Ardito A, Kroiss M, Leboulleux S, Daffara F, Perotti P, Feelders

RA, deVries JH, Zaggia B, De Francia S, Volante M, Haak HR, Allolio B, Al Ghuzlan A,

Fassnacht M, Berruti A. Mitotane levels predict the outcome of patients with adrenocortical

carcinoma treated adjuvantly following radical resection. Eur J Endocrinol., 2013,

169(3):263-70.

D'Avolio A, De Francia S, Basile V, Cusato J, De Martino F, Pirro E, Piccione F, Ardito

A, Zaggia B, Volante M, Di Perri G, Terzolo M. Influence of the CYP2B6 polymorphism

on the pharmacokinetics of mitotane. Pharmacogenet Genomics. 2013, 23(6):293-300.

Serdiuk T, Lysenko V, Mognetti B, Skryshevsky V, Géloën A. Impact of cell division on

intracellular uptake and nuclear targeting with fluorescent SiC-based nanoparticles. J

Biophotonics. 2013 Mar;6(3):291-7. Epub 2012 Jun 1

78

PSYCHIATRY

Dr. Rocco Luigi

PICCI Assistant Professor

Dr. Francesco

OLIVA

Assistant Professor


Quality of life and co-occurrence of psychiatry disorders among patients with alcohol use disorder

- Use and abuse of “Legal highs”, bath salts, and other synthetic cathinones

- Peer-education among high school children to prevent alcohol and substances use/abuse

- Relationship between Type-D (distress) personality and essential hypertension

- Evaluation of the level of pertinence of Compulsory Admission

- Homocysteine blood level and mixed features mood disorder

- The role of glial cell and glutamate pathway in the development of psychiatric disorder

79

Recent Publications (2013/2014) Oliva F, Dalmotto M, Pirfo E, Furlan PM, Picci RL (2014) A comparison of thought and

perception disorders in borderline personality disorder and schizophrenia: psychotic

experiences as a reaction to impaired social functioning, BMC Psychiatry, pp. 14:239

Picci RL, Oliva F, Zuffranieri M, Vizzuso P, Ostacoli L, Sodano AJ, Furlan PM (2014)

Quality of life, alcohol detoxification and relapse: Is quality of life a predictor of relapse or

only a secondary outcome measure?, Quality Of Life Research (ISSN:0962-9343), pp. 1- 11.

Cavallo M, Trivelli F, Adenzato M, Bidoia E, Giaretto RM, Oliva F, Ostacoli L, Sala A, Picci

RL (2013) DO Neuropsychological and social cognition abilities in schizophrenia change

after intensive cognitive training? A pilot study, Clinical Neuropsychiatry (ISSN:1724-4935),

pp. 202- 211. Vol. 10.

Picci RL, Oliva F, Trivelli F, Carezana C, Zuffranieri M, Ostacoli L, Furlan PM, Lala R

(2013) Emotional Burden and Coping Strategies of Parents of Children with Rare Diseases,

Journal Of Child And Family Studies (ISSN:1062-1024), pp. 1- 9.

Rosso C, Garombo M, Oliva F, Furlan PM, Picci RL (2014) Efficacia, target e contesti dei

trattamenti per gli autori di reati sessuali, Rassegna Italiana Di Criminologia (ISSN:1121-

1717), Pp. 6- 11. Vol. 1.

Aguglia E, Biggio G, Signorelli MS, Mencacci C (2014) Steering Committee on behalf of the

STIMA-D Investigators Italian Study on Depressive Disorders (Studio Italiano Malattia

Depressiva, or STIMA), pp. 105-10. Vol 47(3).

Munizza C, Argentero P, Coppo A, Tibaldi G, Di Giannantonio M, Picci RL, Rucci P (2013)

Public beliefs and attitudes towards depression in Italy: a national survey, PLoS One. 2013

May 20. Vol 8(5)

Furlan PM, Oliva F, Picci RL (2013) University psychiatry in Italy.Organisation and

integration of university clinics and the National Health Service, Annali Dell'istituto

Superiore Di Sanità (ISSN:0021-2571), pp. 292- 299. Vol. 49.

Picci RL, Versino E, Oliva F, Giaretto RM, Ostacoli L, Trivelli F, Venturello S, Furlan PM

(2013) Does substance use disorder affect clinical expression in first-hospitalization patients

with schizophrenia? Analysis of a prospective cohort, Psychiatry Research (ISSN:0165-

1781), pp. 780- 786. Vol. 210.

Picci RL, Vigna-Taglianti F, Oliva F, Mathis F, Salmaso S, Ostacoli L, Sodano AJ, Furlan

PM (2012) Personality disorders among patients accessing alcohol detoxification treatment:

prevalence and gender differences, Comprehensive Psychiatry (ISSN:0010-440X), pp. 355-

363. Vol. 53.

80

ROLE OF IRON METABOLISM PROTEINS IN HEMATOLOGIC

MALIGNANCIES

Dr.Marco

DE GOBBI Assistant Professor

Dr. Augusta

DI SAVINO Postdoctoral

Fellowship

Dr. Antonietta

PALMIERI Fellowship


Role of TFR2 in myeloproliferative and myelodysplastic disorders

Transcriptional regulation of TFR2 alpha and beta

Molecular basis of iron overload disorders

81


Palmieri A, Volpengo A, Di Savino A, Carnuccio F, Crasto F, Daraio F, Lorenzatti R, Nicoli

P, Serra A, Morotti A, Saglio G, Roetto A, De Gobbi M. Tranferrin Receptor 2 gene is highly

expressed in peripheral blood cells of patients with polycythemia vera but not with secondary

erythrocytosis. Poster presentation at European Iron Club Meeting Verona 11th-14th

September 21014

Ferbo L, Manzini P, Badar S, Campostrini N, Zamperin G, Delledonne M, Francisi T, Tassi

V, D’Antico S, Girelli D, Roetto A, De Gobbi M. A rare mutation in FPN1 gene found in a

severe iron overloaded Italian patient. Poster presentation at European Iron Club Meeting

Verona 11th-14th September 21014

82

ANNUAL REPORT 2014Current research focuses on the cellular and molecular mechanisms underlying the pathogenesis to of Cerebral Cavernous Malformations (CCM), a major cerebrovascular

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