ANAT2341 Lecture 7 - Beverdam - Mesoderm Development · No screen snap shots or copying of quiz questions ... Dermatome Development Embryonic dermatomes will form the ... ANAT2341

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Mesoderm Development

DrAnnemiekBeverdam– SchoolofMedicalSciences,UNSWWallaceWurth BuildingRoom234– [email protected]

Resources:http://php.med.unsw.edu.au/embryology/

Larsen’s Human EmbryologyThe Developing Human: Clinically Oriented Embryology

Week 3 Lecture overview

DrAnnemiekBeverdam– SchoolofMedicalSciences,UNSWWallaceWurth BuildingRoom234– [email protected]

Gastrulation

Early Mesoderm Development

Notochord

Paraxial Mesoderm

Intermediate Mesoderm

Lateral Plate Mesoderm

GastrulationWeek 3

Ingression of epiblast cells: EMT transitionGeneration of definitive endoderm

Generation of intra-embryonic mesodermOropharyngeal and cloacal membrane

Embryonic ectoderm

http://php.med.unsw.edu.au/embryology/images/5/55/Mesoderm_001.mp4

End product gastrulation:Trilaminar embryo

Ectoderm (Neural crest)brain, spinal cord, eyes, peripheral nervous system

epidermis of skin and associated structures, melanocytes, cranial connective tissues (dermis)

Mesodermmusculo-skeletal system

limbsconnective tissue of skin and organsurogenital system, heart, blood cells

Endodermepithelial linings of gastrointestinal and respiratory tracts

Page342S.F.GilbertDevelopmentalBiology5thedition

Embryonic development:

EarlyMesodermDevelopment

EarlyMesodermDevelopment

1 23 4

NeuralTube

1:notochord2:paraxialmesoderm3:intermediatemesoderm4:lateralplatemesoderm

EarlyMesodermDevelopment

1 23 4

NeuralTube

1:notochord2:paraxialmesoderm3:intermediatemesoderm4:lateralplatemesoderm

1:Notochord

AxialmesodermTransient

DevelopmentNeurenteric canal

Crucialsignalling centreMechanicalroleinembryonicfolding

Notochordal plate

Hollow

Solid

1:Notochord

EarlyMesodermDevelopment

1 23 4

NeuralTube

1:notochord2:paraxialmesoderm3:intermediatemesoderm4:lateralplatemesoderm

2:ParaxialMesoderm

Cranial:Unsegmentedparaxialmesoderm:headmesenchyme

Trunk:Segmentedparaxialmesoderm:somites

2:ParaxialMesodermSomitogenesis

Block-likebilateralcondensationsoftheparaxialmesodermFormevery90minuts inacranialtocaudaldirection(day20today30)

‘Segmentationclock’dependsonHes7 transcription/translation44maxareformed,33remain

Giverisetoaxialskeletonandmusculature,dermisofthetrunk

2:ParaxialMesodermSomiteDevelopment

Somites developinto:- Sclerotome:mesenchymalcells(vertebralbodyandintervertebraldisk)- Dermomyotome:columnarepithelium

Dermomyotome developsinto:- Dermatome:dermisofthetrunk- Myotome:trunkmusculature

2:ParaxialMesodermSomiteDevelopment

Sonichedgehog(Shh)(notochordandfloorplate):ventralsomites.BMP-4:lateralsomites.Wnt familyproteins(roofplate):dorsalsomites.

2:ParaxialMesodermSomiteDerivativeSpecificationdependsonAPlevel/Hox code

7:cervicalregion

12:thoracicregion

5:lumbarregion

5:sacralregion

4:caudalregion(tail)

2:ParaxialMesodermSomiteDevelopment

2:ParaxialMesodermMyotomeDevelopment

Epaxial myotome:epimere:erectorspinaeHypoaxial myotome:hypomere:3primarymusclelayers

MyoD initiatesmyogenesis

2:ParaxialMesodermMyotomeDevelopment

2:ParaxialMesodermDermatomeDevelopment

EmbryonicdermatomeswillformthedermisPostnataldermatomeisastripofskininnervatedbyasinglespinalnerve

EarlyMesodermDevelopment

1 23 4

NeuralTube

1:notochord2:paraxialmesoderm3:intermediatemesoderm4:lateralplatemesoderm

3:IntermediateMesodermSegmentedandunsegmentedintermediatemesoderm

MesonephricductUrogenitalsinus

MesonephrictubulesUretericbuds

3:IntermediateMesoderm3nephricsystems:- Pronephros:regress- Mesonephros:reproductivesystem,andcollectingductandtubulesofthekidney- Metanephros:nephronsofthekidney

EarlyMesodermDevelopment

1 23 4

NeuralTube

1:notochord2:paraxialmesoderm3:intermediatemesoderm4:lateralplatemesoderm

4:LateralPlateMesoderm

Lateralplatemesodermdevelopsinto:- Splanchnic/visceralmesoderm- Somatic/parietalmesoderm

Intraembryoniccoelom:3cavities:- Pericard- Pleural- Peritoneal

Somatic/parietalmesoderm:somatopleure- Closesttoectoderm- Givesriseto:

• Connectivetissueandliningofthebodywall• Bones,ligamentsanddermisofthelimbs

Splanchnic/visceralmesoderm:splanchnopleure- Closesttoendoderm- Givesriseto:

• Cardiacmesoderm(prechordal splanchnicmesoderm)• Bloodvessels• Smoothmusclesofthegut

4:LateralPlateMesoderm

Page342S.F.GilbertDevelopmentalBiology5thedition

Embryonic development:

Week 3 Lecture overview

DrAnnemiekBeverdam– SchoolofMedicalSciences,UNSWWallaceWurth BuildingRoom234– [email protected]

Placentation

Body axes

Gastrulation

Axis formation

Embryo folding

Lecture contents relevant to Labs 3 and 7

Resources:http://php.med.unsw.edu.au/embryology/

Larsen’s Human EmbryologyThe Developing Human: Clinically Oriented Embryology