www.cellsignal.com/ampk For pathway key and background please visit: Other RTKs α-Adrenergic Receptor AMPKα β γ PFKFB3 LKB1 MO25α STRAD Adiponectin Receptor Leptin PKA Ras Rheb PLCβ Gα q SirT1 GS TBC1D1 ACC CPT1 Malonyl CoA CaMKK HMG-CoA Reductase FIP200 TSC2 TSC1 PGC1α Insulin SREBP-1 Fatty Acid Synthase HSL ATGL Akt B-Raf Erk p90RSK PI3K Insulin Receptor p70 S6K 4E-BP1 Beclin mTOR GβL Raptor ULK1 PI3K Class III ATG13 Atg14 p300 HNF4α TORC2/ CRTC2 CREB FoxO HDAC7 HDAC5 HDAC4 CRY1 Glut4 vesicle Glut1 vesicle Sterol/Isoprenoid Synthesis Fatty Acid Oxidation Lipolysis Glycolysis Glycogen Synthesis [cAMP] Low Glucose, Hypoxia, Ischemia, Heat Shock AICAR Exercise Brain [Ca 2+ ] Histamine Thrombin Glut Trafficking Glut4 Glut1 Autophagy Lipogenesis AMP + ADP ATP Gluconeogenesis Glucose Glut Translocation Microtubules Actin Dynamics MBS85 MYPT1 TXNIP Myosin Light Chain2 CLIP170 Tau C ytoskeletal Signaling A c u t e R e g u l a t i o n o f M e t a b o li s m Transcriptional C ontrol of M eta b olis m P r o t e in M e ta b o lis m a n d A u t o p h a g y Energy Metabolism AMPK Signaling Pathway
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www.cellsignal.com/ampkFor pathway key and background please visit:
Other RTKsα-AdrenergicReceptor
AMPKαβ γ
PFKFB3
LKB1MO25αSTRAD
Adiponectin Receptor
Leptin
PKA Ras
Rheb
PLCβ Gαq
SirT1
GS TBC1D1
ACC
CPT1Malonyl
CoA
CaMKK
HMG-CoAReductase
FIP200
TSC2TSC1
PGC1α
Insulin
SREBP-1
Fatty AcidSynthase
HSL
ATGL
Akt
B-Raf
Erk
p90RSK
PI3K
InsulinReceptor
p70S6K
4E-BP1
Beclin
mTOR GβLRaptor
ULK1
PI3KClass III
ATG13
Atg14
p300
HNF4α
TORC2/CRTC2
CREB
FoxO
HDAC7HDAC5HDAC4
CRY1
Glut4vesicle
Glut1vesicle
Sterol/IsoprenoidSynthesis
Fatty AcidOxidation
Lipolysis
Glycolysis
GlycogenSynthesis
[cAMP]
Low Glucose,Hypoxia, Ischemia,
Heat ShockAICAR
ExerciseBrain
[Ca2+]
HistamineThrombin
GlutTrafficking
Glut4Glut1
Autophagy
Lipogenesis
AMP + ADPATP
Gluconeogenesis
Glucose
Glut Translocation
Microtubules
ActinDynamics
MBS85MYPT1
TXNIP
MyosinLight
Chain2
CLIP170Tau
Cytoskeletal Signaling
Acute Regulation of M
etabolism
Transcriptional Control of Metabolism
Prot
ein
Met
abol
ism a
nd A
utop
hagy
Energy Metabolism
AMPK Signaling Pathway
www.cellsignal.com/ampkFor Research Use Only. Not For Use In Diagnostic Procedures.
Phospho-AMPKα (Thr172) (40H9) Rabbit mAb #2535: WB analysis of extracts from 293 cells, untreated (-) or treated with Oligomycin #9996 (0.5 µM, 30 min; +), using #2535 (upper) or AMPKα Antibody #2532 (lower).
kDa
LKB1
CAKICOS
L929
PC12
140
10080
6050
40
LKB1 (D60C5) Rabbit mAb #3047: WB analysis of extracts from various cell lines using #3047.
Visit www.cellsignal.com/ampklist for full listing of antibodies, related kits, and reagents.
COX IV (3E11) Rabbit mAb #4850: Confocal IF analysis of HeLa cells using #4850 (green). Actin filaments were labeled with Alexa Fluor® 555 Phalloidin #8953 (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
SDHA (D6J9M) XP® Rabbit mAb #11998: IHC analysis of paraffin-embedded human colon carcinoma using #11998 in the presence of control peptide (top) or antigen-specific peptide (bottom).
APPLICATIONS & REACTIVITY KEY: WB Western Blotting / IP Immunoprecipitation / IHC Immunohistochemistry / IF Immunofluorescence / F Flow Cytometry / ChIP Chromatin Immunoprecipitation / -IC Immunocytochemistry / -P Paraffin / -F Frozen / E-P Peptide ELISA / H human / M mouse / R rat / Hm hamster / Mk monkey / C chicken / Mi mink / Dm D. melanogaster / X Xenopus / Z zebra fish / B bovine / Dg dog / Pg pig / Sc S. cerevisiae / All all species expected / ( ) 100% sequence homology