February 2012 ilyx Biotechnologies 18, 2022 -CONFIDENTIAL OptiSol Reagent Ki FASOS Technology
Dec 19, 2014
February 2012© Dilyx Biotechnologies Apr 10, 2023 NON-CONFIDENTIAL
OptiSol Reagent Kit
FASOS Technology
Why use OptiSol?
Ensure maximal protein sample utility
Avoid #1 cause of protein sample loss: protein aggregation
Find formulations that protect target protein
from aggregation solubilize aggregated
protein sample Convert problematic
proteins into ‘well-behaved’ research tools
© Dilyx Biotechnologies Apr 10, 2023 NON-CONFIDENTIAL 2
OptiSol™ Product Components
Kit box: Reagent Plate (96 formulations) Reaction Plate Filter Plate (30, 200 kDa MWCO) Collection Plate Quick-start guide (brochure) Manual & MSDS
Evaluation tool: Protein Dashboard Password & URL for web-download
© Dilyx Biotechnologies Apr 10, 2023 NON-CONFIDENTIAL 3
OptiSol Product SpecificationCategory Product Details
Product availability
OptiSol 30 or OptiSol 200
Kit box dimensions
7 x 3 x 4 inches
Kit box weight 14 oz (400 g)
Kit components OptiSol Reagent Plate™ (96 formulations, 160 L each) OptiSol Reaction Plate™OptiSol Filter Plate™ (30 kDa MWCO or 200 kDa MWCO) OptiSol Collection Plate™
Analytical software tool
Protein Dashboard™ is a data evaluation tool that supports the interpretation of results (requires MS Excel)
Sample requirements
Recommended Sample Volume: 1-20 L / well (100 L – 2 L protein solution)Recommended Protein Concentration: 1 mg/mL or less (Protein concentration used and protein detection assay need to be matched)
Use requirements
Assay: User provides protein assay. Assay sensitivity needs to be sufficient to detect less than 1/10 of original protein concentration. Examples: Bradford, UV280, ELISA, WESTERN, etc.Equipment: Centrifuge with swing bucket rotor that accepts SBS-sized plate stack
Application A: Protein solubility profilingB: Solubilization of aggregated protein sample.
Shipping Shipped at ambient temperature
Storage Recommended temperature: Reagent Plate (4˚C), rest of kit: room temperature
Stability Kit components are stable for 6 months after receipt
© Dilyx Biotechnologies Apr 10, 2023 NON-CONFIDENTIAL 4
OptiSol is based on FASOS Technology
FASOS = Filter Aided Solubility Optimization Screening 2 OptiSol Applications
Patent pending
A: Protein Solubility Profiling B: Solubilization of Protein Aggregates
© Dilyx Biotechnologies Apr 10, 2023 NON-CONFIDENTIAL 5
Sample Stress Examples
© Dilyx Biotechnologies Apr 10, 2023 NON-CONFIDENTIAL 6
Compatible Analytical Tests
© Dilyx Biotechnologies Apr 10, 2023 NON-CONFIDENTIAL 7
Simplified Data Evaluation with the Protein Dashboard™
© Dilyx Biotechnologies Apr 10, 2023 NON-CONFIDENTIAL 8
OptiSol Performance
© Dilyx Biotechnologies Apr 10, 2023 NON-CONFIDENTIAL 9
OptiSol Product Pricing
© Dilyx Biotechnologies Apr 10, 2023 NON-CONFIDENTIAL 10
DILYX BIOTECHNOLOGIES, LLC
Feb 2012© Dilyx Biotechnologies Apr 10, 2023 NON-CONFIDENTIAL
Dilyx Biotechnologies, LLC815 First Ave #168
Seattle WA 98104
FAX: 202-207-0398Phone:425-205-0588