iba Solutions For Life Sciences www.iba-lifesciences.com MAGSTREP “TYPE3” XT BEADS – magnetic Strep-tag purification at its best! Product information Strep-tag Magnetic beads for high demands ! high specificity – low background – even for elution by boiling high binding capacity high affinity for Strep-tag
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ag trep type3” Xt beadS - bertin-bioreagent.com€œtype3” XT Beads 2 ml 2-1040-050 Buffer BX; Biotin Elution Buffer (5x) e information: ep-tag.com. Mag. Strep “type3” XT Beads.
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iba Solutions For Life Sciences
www.iba-lifesciences.com
MagStrep “type3” Xt beadS– magnetic Strep-tag purification at its best!
MagStrep “type3” XT Beads are Strep-Tactin XT coated ferri-magnetic spheres with the following characteristics:
high binding capacity (1 – 3 nmol/μl beads, corresponding to 30 – 90 μg of a 30 kDa protein) very low non-specific protein binding due to improved coating
flexible elution conditions, under denaturing conditions by boiling in SDS gel loading buffer or under native conditions with biotin
high affinity for Strep-tag – due to new Strep-Tactin XT
These characteristics lead to very good purification results (see Fig.1) and make the MagStrep “type3” XT beads a superior tool for small scale purifications with high demands on protein purity.
GFP
-Strep-
tag
Crude bacterial extract before purification
Fig 1: Purification of GFP-Strep-tag fusion protein from crude bact. extract using MagStrep “type3” XT beads (Protein purification analysis was performed with an Agilent Bioanalyzer 2100 system instead of SDS-PAGE)
Native purification using buffer BX for elution
GFP
-Strep-
tag
Strep-
Tact
in X
T
Denaturing purification using sample buffer and boiling for elution
Purity >95%
Purity >95%
Due to specific binding properties of the beads even the elution by boiling leads to highly pure proteins (GFP peak at ~28kDa ignoring Strep-Tactin XT peak)