Affymetrix 2.0 miRNA arrays on lung tissue RNA n=3-4 mice/strain 92 differentially expressed miRNAs 38 miRNAs both differentially and highly expressed miRNA eQTL mapping Identify quantitative trait transcripts and quantitative trait miRNAs miRhub analyses** Colocalization of eQTL and inflammation (eosinophil, neutrophil) QTL? CC Founder Strains Exiqon locked nucleic acid based qRT-PCR of 38 miRNAs on 129 preCC mice 18 eQTL detected + 8 suggestive eQTL miR-31, miR-351, and miR-497 targets enriched among neutrophil quantitative trait transcripts Inflammation phenotype Expression mRNA microRNA OR Inflammation phenotype Expression microRNA mRNA No* Identification of putative causal variants for miR-342-3p eQTL “eQTL Approach” “Bioinformatic/ Statistical Approach” Combine with genotype data Combine with inflammatio n phenotype data Supplementary Figure 1.
Affymetrix 2.0 miRNA arrays on lung tissue RNA n=3-4 mice/strain 92 differentially expressed miRNAs 38 miRNAs both differentially and highly expressed.
Dec 13, 2015
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Affymetrix 2.0 miRNA arrays on lung tissue RNAn=3-4 mice/strain
92 differentially expressed miRNAs38 miRNAs both differentially and highly expressed
miRNA eQTL mappingIdentify quantitative trait transcripts
and quantitative trait miRNAs
miRhub analyses**Colocalization of eQTL and inflammation (eosinophil,
neutrophil) QTL?
CC Founder Strains
Exiqon locked nucleic acid based qRT-PCR of 38 miRNAs on 129 preCC mice
18 eQTL detected+ 8 suggestive eQTL
miR-31, miR-351, and miR-497 targetsenriched among neutrophil quantitative trait transcripts
Infla
mm
atio
np
hen
oty
pe
Expression
mRNA
microRNA
OR
Infla
mm
atio
np
hen
oty
pe
Expression
microRNA
mRNA
No*
Identification of putative causal variants
for miR-342-3p eQTL
“eQTL Approach”
“Bioinformatic/StatisticalApproach”
Combine with genotype data
Combine with inflammation phenotype data
Supplementary Figure 1.
Supplementary Figure 2.
Supplementary Figure 3.
A B
Supplementary Figure 4.
Supplementary Figure 5.
miR-322miR-203 miR-221
miR-503miR-322* miR-351
miR-146b miR-181d miR-187
Supplementary Figure 6.
Supplementary Figure 7.
Enrichment Among Positive Quantitative Trait Transcripts
Enrichment Among Negative Quantitative Trait Transcripts
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