Advances in faba bean genetics and genomics Article Accepted Version O'Sullivan, D. M. and Angra, D. (2016) Advances in faba bean genetics and genomics. Frontiers in Genetics, 7. 150. ISSN 1664-8021 doi: https://doi.org/10.3389/fgene.2016.00150 Available at http://centaur.reading.ac.uk/67667/ It is advisable to refer to the publisher’s version if you intend to cite from the work. Published version at: http://dx.doi.org/10.3389/fgene.2016.00150 To link to this article DOI: http://dx.doi.org/10.3389/fgene.2016.00150 Publisher: Frontiers Media All outputs in CentAUR are protected by Intellectual Property Rights law, including copyright law. Copyright and IPR is retained by the creators or other copyright holders. Terms and conditions for use of this material are defined in the End User Agreement . www.reading.ac.uk/centaur CentAUR Central Archive at the University of Reading
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Advances in faba bean genetics and genomics Article
Accepted Version
O'Sullivan, D. M. and Angra, D. (2016) Advances in faba bean genetics and genomics. Frontiers in Genetics, 7. 150. ISSN 16648021 doi: https://doi.org/10.3389/fgene.2016.00150 Available at http://centaur.reading.ac.uk/67667/
It is advisable to refer to the publisher’s version if you intend to cite from the work. Published version at: http://dx.doi.org/10.3389/fgene.2016.00150
To link to this article DOI: http://dx.doi.org/10.3389/fgene.2016.00150
Publisher: Frontiers Media
All outputs in CentAUR are protected by Intellectual Property Rights law, including copyright law. Copyright and IPR is retained by the creators or other copyright holders. Terms and conditions for use of this material are defined in the End User Agreement .
Donal M. O’Sullivan, Deepti Angra. 3 School of Agriculture, Policy and Development, 4 University of Reading, 5 PO Box 237, 6 Whiteknights, 7 Reading, 8 RG6 6AR, 9 United Kingdom. 10
Figure 1: Integrative Genomics Viewer (IGV) snapshot of SNPs in the Vf orthologue of 471
Medtr2g009080 mined from public trancriptome datasets.The eleven horizontal tracks shown in this 472
view from top to bottom are: the meta-transcriptome contig TR55082¦c0_g4_i2 is shown as a 473
schematic, all intravarietal SNP positions; positions where the minor (non-consensus) allele is found 474
in each of the following genotypes: ‘Albus’, ‘Fiord’, ‘SRX641218’, ‘SSN1’, ‘Fatima’, A01155’ and 475
‘BPL10’, alignment of the reference Vf contig TR55082¦c0_g4_i2 to the Mt4.0 genome (coding 476
region of Medtr2g009080 in this instance), and in the bottom panel is shown a 3-frame translation of 477
the Vf sequence. In this example, there are a total of 16 intravarietal SNPs distributed across the gene - 478
eleven unique to ‘Fiord’, two unique to ‘A001155’ and three minor alleles in common to ‘Fiord’ and 479
‘A01155’, with the remaining genotypes carrying the unchanged common allele identical to the 480
consensus contig sequence. 481
Figure 2: Wheel representation of synteny between pseudomolecule sequence of Medicago truncatula 482
chromosomes Mt1-Mt8 and the consensus Vf linkage map (Vf1-Vf6). Syntenic relationships were as 483
described in Webb et al., 2016 and re-drawn for this figure using ‘Circos’ software (Krzywinski et al., 484
2009). Link lines to Medicago orthologues for markers on each Vf chromosome are shown in a 485
different colour. The virtually uninterrupted collinearity between the whole of Vf3 and the whole of 486
Mt1 is highlighted by representing these orthologous chromosomes in green. To illustrate how useful 487
this syntenic framework is for translational genomics as well as for drawing together knowledge of 488
the genome, three exemplar loci where there is a proven or presumed orthologous relationship are 489
shown as red ticks on the orthologous chromosomes and linked by black lines. These are: the mapped 490
ZT1 locus and its Medicago orthologue MtWD40-1, the mapped genetic interval corresponding to the 491
mapped VC (vicine-convicine) locus and its corresponding Medicago syntenic interval on Mt2, and 492
the actual position of the Medicago TERMINAL FLOWER LOCUS 1 (TFL1) with a link drawn to the 493
region of Vf1 where it is presumed the Vf orthologue (VfTFL1) is likely to be located. 494
Figure 3: Mutant phenotypes observed in X-ray and EMS M2 lines grown at the University of 495
Reading in 2015. A. disease lesion mimic B. determinate flowering B. curled wing petal C. disease 496
lesion mimic D. reduced flower pigmentation. 497
498
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References 499
500
Abdelwahd, R., Udupa, S.M., Gaboun, F., Diria, G., Mentag, R., Ibriz, Mohamed and Iraqi, D. (2014) 501 Agrobacterium-mediated transformation of cotyledonary node of Vicia faba L. Rom Agric 502 Res 31, online first. 503
Arun-Chinnappa, K.S. and McCurdy, D.W. (2015) De novo assembly of a genome-wide 504 transcriptome map of Vicia faba (L.) for transfer cell research. Front Plant Sci 6. 505
Avila, C.M., Atienza, S.G., Moreno, M.T. and Torres, A.M. (2007) Development of a new diagnostic 506 marker for growth habit selection in faba bean (Vicia faba L.) breeding. Theor Appl Genet 507 115, 1075-1082. 508
Avila, C.M., Satovic, Z., Sillero, J.C., Rubiales, D., Moreno, M.T. and Torres, A.M. (2004) Isolate 509 and organ-specific QTLs for ascochyta blight resistance in faba bean (Vicia faba L). Theor 510 Appl Genet 108, 1071-1078. 511
Avila, C.M., Sillero, J.C., Rubiales, D., Moreno, M.T. and Torres, A.M. (2003) Identification of 512 RAPD markers linked to the Uvf-1 gene conferring hypersensitive resistance against rust 513 (Uromyces viciae-fabae) in Vicia faba L. Theor Appl Genet 107, 353-358. 514
Bishop, J., Jones, H.E., Lukac, M. and Potts, S.G. (2016a) Insect pollination reduces yield loss 515 following heat stress in faba bean (Vicia faba L.). Agriculture, Ecosystems & Environment 516 220, 89-96. 517
Bishop, J., Potts, S.G. and Jones, H.E. (2016b) Susceptibility of Faba Bean (Vicia faba L.) to Heat 518 Stress During Floral Development and Anthesis. J Agron Crop Sci, n/a-n/a. 519
Böttinger, P., Steinmetz, A., Schieder, O. and Pickardt, T. (2001) Agrobacterium-mediated 520 transformation of Vicia faba. Mol Breeding 8, 243-254. 521
Cernay, C., Ben-Ari, T., Pelzer, E., Meynard, J.-M. and Makowski, D. (2015) Estimating variability 522 in grain legume yields across Europe and the Americas. Sci Rep-Uk 5, 11171. 523
Cottage, A., Gostkiewicz, K., Thomas, J.E., Borrows, R., Torres, A.M. and O'Sullivan, D.M. (2012) 524 Heterozygosity and diversity analysis using mapped single nucelotide polymorphisms in a 525 faba bean inbreeding programme. Mol Breeding 30, 1799-1809. 526
Crepon, K., Marget, P., Peyronnet, C., Carrouee, B., Arese, P. and Duc, G. (2010) Nutritional value of 527 faba bean (Vicia faba L.) seeds for feed and food. Field Crop Res 115, 329-339. 528
Cruz-Izquierdo, S., Avila, C.M., Satovic, Z., Palomino, C., Gutierrez, N., Ellwood, S.R., Phan, 529 H.T.T., Cubero, J.I. and Torres, A.M. (2012) Comparative genomics to bridge Vicia faba with 530 model and closely-related legume species: stability of QTLs for flowering and yield-related 531 traits. Theor Appl Genet 125, 1767-1782. 532
Denton, M.D., Pearce, D.J. and Peoples, M.B. (2013) Nitrogen contributions from faba bean (Vicia 533 faba L.) reliant on soil rhizobia or inoculation. Plant Soil 365, 363-374. 534
Diaz-Ruiz, R., Satovic, Z., Avila, C.M., Alfaro, C.M., Gutierrez, M.V., Torres, A.M. and Roman, B. 535 (2009) Confirmation of QTLs controlling Ascochyta fabae resistance in different generations 536 of faba bean (Vicia faba L.). Crop Pasture Sci 60, 353-361. 537
Diaz-Ruiz, R., Torres, A.M., Satovic, Z., Gutierrez, M.V., Cubero, J.I. and Roman, B. (2010) 538 Validation of QTLs for Orobanche crenata resistance in faba bean (Vicia faba L.) across 539 environments and generations. Theor Appl Genet 120, 909-919. 540
Duc, G. (1995) Mutagenesis of Faba Bean (Vicia-Faba L) and the Identification of 5 Different Genes-541 Controlling No Nodulation, Ineffective Nodulation or Supernodulation. Euphytica 83, 147-542 152. 543
Duc, G., Bao, S.Y., Baum, M., Redden, B., Sadiki, M., Suso, M.J., Vishniakova, M. and Zong, X.X. 544 (2010) Diversity maintenance and use of Vicia faba L. genetic resources. Field Crop Res 115, 545 270-278. 546
Duc, G., Moussy, F., Zong, X. and Ding, G. (1999) Single gene mutation for green cotyledons as a 547 marker for the embryonic genotype in faba bean, Vicia faba. Plant Breeding 118, 577-578. 548
Duc, G. and Picard, J. (1986) Note on the Presence of the Sym-1 Gene in Vicia-Faba Hampering Its 549 Symbiosis with Rhizobium-Leguminosarum. Euphytica 35, 61-64. 550
Page 18 of 21
El-Rodeny, W., Kimura, M., Hirakawa, H., Sabah, A., Shirasawa, K., Sato, S., Tabata, S., Sasamoto, 551 S., Watanabe, A., Kawashima, K., Kato, M., Wada, T., Tsuruoka, H., Takahashi, C., Minami, 552 C., Nanri, K., Nakayama, S., Kohara, M., Yamada, M., Kishida, Y., Fujishiro, T. and Isobe, 553 S. (2014) Development of EST-SSR markers and construction of a linkage map in faba bean 554 (Vicia faba). Breeding Sci 64, 252-263. 555
Ellwood, S.R., Phan, H.T.T., Jordan, M., Hane, J., Torres, A.M., Avila, C.M., Cruz-Izquierdo, S. and 556 Oliver, R.P. (2008) Construction of a comparative genetic map in faba bean (Vicia faba L.); 557 conservation of genome structure with Lens culinaris. Bmc Genomics 9. 558
Erith, A.G. (1930) The inheritance of colour, size and form of seeds, and of flower colour in Vicia 559 Faba L. Genetica 12, 477-510. 560
Gaj, T., Gersbach, C.A. and Barbas Iii, C.F. (2013) ZFN, TALEN, and CRISPR/Cas-based methods 561 for genome engineering. Trends in Biotechnology 31, 397-405. 562
Gnanasambandam, A., Paull, J., Torres, A., Kaur, S., Leonforte, T., Li, H., Zong, X., Yang, T. and 563 Materne, M. (2012) Impact of Molecular Technologies on Faba Bean (Vicia faba L.) 564 Breeding Strategies. Agronomy 2, 132-166. 565
Gong, Y.M., Xu, S.C., Mao, W.H., Li, Z.Y., Hu, Q.Z., Zhang, G.W. and Ding, J. (2011) Genetic 566 Diversity Analysis of Faba Bean (Vicia faba L.) Based on EST-SSR Markers. Agr Sci China 567 10, 838-844. 568
Gressel, J. (2009) Crops with target-site herbicide resistance for Orobanche and Striga control. Pest 569 Manag Sci 65, 560-565. 570
Gutierrez, N., Palomino, C., Satovic, Z., Ruiz-Rodriguez, M.D., Vitale, S., Gutierrez, M.V., Rubiales, 571 D., Kharrat, M., Amri, M., Emeran, A.A., Cubero, J.I., Atienza, S.G., Torres, A.M. and Avila, 572 C.M. (2013) QTLs for Orobanche spp. resistance in faba bean: identification and validation 573 across different environments. Mol Breeding 32, 909-922. 574
Hanafy, M., Pickardt, T., Kiesecker, H. and H.-J., J. (2005) Agrobacterium-mediated transformation 575 of faba bean (Vicia faba L.) using embryo axes. Euphytica 142, 227-236. 576
Hanafy, M.S., El-Banna, A., Schumacher, H.M., Jacobsen, H.J. and Hassan, F.S. (2013) Enhanced 577 tolerance to drought and salt stresses in transgenic faba bean (Vicia faba L.) plants by 578 heterologous expression of the PR10a gene from potato. Plant Cell Rep 32, 663-674. 579
Kaur , S., Cogan , N.O.I., Forster , J.W. and Paull, J.G. (2014) Assessment of Genetic Diversity in 580 Faba Bean Based on Single Nucleotide Polymorphism. Diversity 6, 88-101. 581
Kaur, S., Kimber, R.B.E., Cogan, N.O.I., Materne, M., Forster, J.W. and Paull, J.G. (2014) SNP 582 discovery and high-density genetic mapping in faba bean (Vicia faba L.) permits 583 identification of QTLs for ascochyta blight resistance. Plant Sci 217, 47-55. 584
Kaur, S., Pembleton, L.W., Cogan, N.O.I., Savin, K.W., Leonforte, T., Paull, J., Materne, M. and 585 Forster, J.W. (2012) Transcriptome sequencing of field pea and faba bean for discovery and 586 validation of SSR genetic markers. Bmc Genomics 13. 587
Khamassi, K., Ben Jeddi, F., Hobbs, D., Irigoyen, J., Stoddard, F., O'Sullivan, D.M. and Jones, H. 588 (2013) A baseline study of vicine-convicine levels in faba bean (Vicia faba L.) germplasm. 589 Plant Genet Resour-C 11, 250-257. 590
Khan, H.R., Paull, J.G., Siddique, K.H.M. and Stoddard, F.L. (2010) Faba bean breeding for drought-591 affected environments: A physiological and agronomic perspective. Field Crop Res 115, 279-592 286. 593
Khazaei, H., O'Sullivan, D.M., Jones, H., Pitts, N., Sillanpaa, M.J., Parssinen, P., Manninen, O. and 594 Stoddard, F.L. (2015) Flanking SNP markers for vicine-convicine concentration in faba bean 595 (Vicia faba L.). Mol Breeding 35. 596
Khazaei, H., O'Sullivan, D.M., Sillanpaa, M.J. and Stoddard, F.L. (2014) Use of synteny to identify 597 candidate genes underlying QTL controlling stomatal traits in faba bean (Vicia faba L.). 598 Theor Appl Genet 127, 2371-2385. 599
Kovarova, P., Navratilova, A., Macas, J. and Dolezel, J. (2007) Chromosome analysis and sorting in 600 Vicia sativa using flow cytometry. Biol Plantarum 51, 43-48. 601
Krzywinski, M., Schein, J., Birol, İ., Connors, J., Gascoyne, R., Horsman, D., Jones, S.J. and Marra, 602 M.A. (2009) Circos: An information aesthetic for comparative genomics. Genome research 603 19, 1639-1645. 604
Page 19 of 21
Link, W., Balko, C. and Stoddard, F.L. (2010) Winter hardiness in faba bean: Physiology and 605 breeding. Field Crop Res 115, 287-296. 606
Ma, Y., Bao, S.Y., Yang, T., Hu, J.G., Guan, J.P., He, Y.H., Wang, X.J., Wan, Y.L., Sun, X.L., Jiang, 607 J.Y., Gong, C.X. and Zong, X.X. (2013) Genetic linkage map of Chinese native variety faba 608 bean (Vicia faba L.) based on simple sequence repeat markers. Plant Breeding 132, 397-400. 609
Ma, Y., Yang, T., Guan, J.P., Wang, S.M., Wang, H.F., Sun, X.L. and Zong, X.X. (2011) 610 Development and Characterization of 21 Est-Derived Microsatellite Markers in Vicia Faba 611 (Fava Bean). Am J Bot 98, E22-E24. 612
Mao, D., Paull, J., Oldach, K.H., Preston, C., Yang, S.Y., Davies, P. and McMurray, L. (2014) The 613 development of multiple herbicide tolerances in faba bean (Vicia faba L.) through induced 614 mutation. In: 15th Autralasian Plant Breeding Conference. Melbourne, Australia. 615
Maxted, N. (1993) Towards a faba bean progenitor! FABIS Newsletter 31. 616 Menke, M., Meister, A. and Schubert, I. (2000) N-methyl-N-nitrosourea-induced DNA damage 617
detected by the comet assay in Vicia faba nuclei during all interphase stages is not restricted 618 to chromatid aberration hot spots. Mutagenesis 15, 503-506. 619
Milan, P.R. and Upadhyay, S. (2007) Impact of food additives on mitotic chromosomes of Vicia faba 620 L. Caryologia 60, 309-314. 621
Mobini, S.H., Lulsdorf, M., Warkentin, T.D. and Vandenberg, A. (2015) Plant growth regulators 622 improve in vitro flowering and rapid generation advancement in lentil and faba bean. In Vitro 623 CellDevBiol-Plant 51, 71-79. 624
Multari, S., Stewart, D. and Russell, W.R. (2015) Potential of Fava Bean as Future Protein Supply to 625 Partially Replace Meat Intake in the Human Diet. Comprehensive Reviews in Food Science 626 and Food Safety, n/a-n/a. 627
Nayak, G.K., Roberts, S.P.M., Garratt, M., Breeze, T.D., Tscheulin, T., Harrison-Cripps, J., 628 Vogiatzakis, I.N., Stirpe, M.T. and Potts, S.G. (2015) Interactive effect of floral abundance 629 and semi-natural habitats on pollinators in field beans (Vicia faba). Agr Ecosyst Environ 199, 630 58-66. 631
Ocaña, S., Seoane, P., Bautista, R., Palomino, C., Claros, G.M., Torres, A.M. and Madrid, E. (2015) 632 Large-Scale Transcriptome Analysis in Faba Bean (Vicia faba L.) under Ascochyta fabae 633 Infection. Plos One 10, e0135143. 634
Patrick, J.W. and Stoddard, F.L. (2010) Physiology of flowering and grain filling in faba bean. Field 635 Crop Res 115, 234-242. 636
Patto, M.C.V., Torres, A.M., Koblizkova, A., Macas, J. and Cubero, J.I. (1999) Development of a 637 genetic composite map of Vicia faba using F-2 populations derived from trisomic plants. 638 Theor Appl Genet 98, 736-743. 639
Pérez-de-Luque, A., Eizenberg, H., Grenz, J.H., Sillero, J.C., Ávila, C., Sauerborn, J. and Rubiales, D. 640 (2010) Broomrape management in faba bean. Field Crop Res 115, 319-328. 641
Preissel, S., Reckling, M., Schläfke, N. and Zander, P. (2015) Magnitude and farm-economic value of 642 grain legume pre-crop benefits in Europe: A review. Field Crop Res 175, 64-79. 643
Ramsay, G. (1997) Inheritance and linkage of a gene for testa-imposed seed dormancy in faba bean 644 (Vicia faba L.). Plant Breeding 116, 287-289. 645
Ray, H., Bock, C. and Georges, F. (2015) Faba Bean: Transcriptome Analysis from Etiolated Seedling 646 and Developing Seed Coat of Key Cultivars for Synthesis of Proanthocyanidins, Phytate, 647 Raffinose Family Oligosaccharides, Vicine, and Convicine. Plant Genome-Us 8. 648
Ray, H. and Georges, F. (2010) A genomic approach to nutritional, pharmacological and genetic 649 issues of faba bean (Vicia faba): prospects for genetic modifications. GM crops 1, 99-106. 650
Rybaczek, D., Zabka, A., Pastucha, A. and Maszewski, J. (2008) Various chemical agents can induce 651 premature chromosome condensation in Vicia faba. Acta Physiol Plant 30, 663-672. 652
Sallam, A., Martsch, R. and Moursi, Y. (2015) Genetic variation in morpho-physiological traits 653 associated with frost tolerance in faba bean (Vicia faba L.). Euphytica 205, 395-408. 654
Sanz, A.M., Gonzalez, S.G., Syed, N.H., Suso, M.J., Saldana, C.C. and Flavell, A.J. (2007) Genetic 655 diversity analysis in Vicia species using retrotransposon-based SSAP markers. Mol Genet 656 Genomics 278, 433-441. 657
(2013) A reference consensus genetic map for molecular markers and economically important 660 traits in faba bean (Vicia faba L.). Bmc Genomics 14. 661
Schmutz, J., Cannon, S.B., Schlueter, J., Ma, J., Mitros, T., Nelson, W., Hyten, D.L., Song, Q., 662 Thelen, J.J., Cheng, J., Xu, D., Hellsten, U., May, G.D., Yu, Y., Sakurai, T., Umezawa, T., 663 Bhattacharyya, M.K., Sandhu, D., Valliyodan, B., Lindquist, E., Peto, M., Grant, D., Shu, S., 664 Goodstein, D., Barry, K., Futrell-Griggs, M., Abernathy, B., Du, J., Tian, Z., Zhu, L., Gill, N., 665 Joshi, T., Libault, M., Sethuraman, A., Zhang, X.C., Shinozaki, K., Nguyen, H.T., Wing, 666 R.A., Cregan, P., Specht, J., Grimwood, J., Rokhsar, D., Stacey, G., Shoemaker, R.C. and 667 Jackson, S.A. (2010) Genome sequence of the palaeopolyploid soybean. Nature 463, 178-668 183. 669
Sillero, J.C., Villegas-Fernández, A.M., Thomas, J., Rojas-Molina, M.M., Emeran, A.A., Fernández-670 Aparicio, M. and Rubiales, D. (2010) Faba bean breeding for disease resistance. Field Crop 671 Res 115, 297-307. 672
Sjödin, J.A.N. (1970) Induced asynaptic mutants in Vicia faba L. Hereditas 66, 215-232. 673 Sjödin, J.A.N. (1971) Induced morphological variation in Vicia faba L. Hereditas 67, 155-179. 674 Sobita, K. and Bhagirath, T. (2005) Effects of some medicinal plant extracts on Vicia faba root tip 675
chromosomes. Caryologia 58, 255-261. 676 Soltis, D.E., Soltis, P.S., Bennett, M.D. and Leitch, I.J. (2003) Evolution of genome size in the 677
angiosperms. Am J Bot 90, 1596-1603. 678 Stoddard, F.L., Nicholas, A.H., Rubiales, D., Thomas, J. and Villegas-Fernández, A.M. (2010) 679
Integrated pest management in faba bean. Field Crop Res 115, 308-318. 680 Suresh, S., Kim, T.-S., Raveendar, S., Cho, J.-H., Yi, J.-Y., Lee, M.C., Lee, S.-Y., Baek, H.-J., Cho, 681
G.-T. and Chung, J.-W. (2015) Transcriptome characterization and large-scale identification 682 of SSR/SNP markers in symbiotic nitrogen fixation crop faba bean (Vicia faba L.). Turk J 683 Agric For 39, 459-469. 684
Tan, S., Evans, R.R., Dahmer, M.L., Singh, B.K. and Shaner, D.L. (2005) Imidazolinone-tolerant 685 crops: history, current status and future. Pest Manag Sci 61, 246-257. 686
Tanno, K. and Willcox, G. (2006) The origins of cultivation of Cicer arietinum L. and Vicia faba L.: 687 early finds from Tell el-Kerkh, north-west Syria, late 10th millennium BP. Veg Hist 688 Archaeobot 15, 197-204. 689
Tavakkoli, E., Paull, J., Rengasamy, P. and McDonald, G.K. (2012) Comparing genotypic variation in 690 faba bean (Vicia faba L.) in response to salinity in hydroponic and field experiments. Field 691 Crop Res 127, 99-108. 692
Till, B.J., Reynolds, S.H., Greene, E.A., Codomo, C.A., Enns, L.C., Johnson, J.E., Burtner, C., 693 Odden, A.R., Young, K., Taylor, N.E., Henikoff, J.G., Comai, L. and Henikoff, S. (2003) 694 Large-scale discovery of induced point mutations with high-throughput TILLING. Genome 695 research 13, 524-530. 696
Torres, A.M., Avila, C.M., Gutierrez, N., Palomino, C., Moreno, M.T. and Cubero, J.I. (2010) 697 Marker-assisted selection in faba bean (Vicia faba L.). Field Crop Res 115, 243-252. 698
Torres, A.M., Weeden, N.F. and Martin, A. (1993) Linkage among isozyme, RFLP and RAPD 699 markers in Vicia faba. Theor Appl Genet 85, 937-945. 700
van de Wouw, M., Enneking, D., Robertson, L.D. and Maxted, N. (2001) Vetches. In: Plant Genetics 701 Resources of Legumes in the Mediterranean pp. 134-158. 702
Wang, H.F., Zong, X.X., Guan, J.P., Yang, T., Sun, X.L., Ma, Y. and Redden, R. (2012a) Genetic 703 diversity and relationship of global faba bean (Vicia faba L.) germplasm revealed by ISSR 704 markers. Theor Appl Genet 124, 789-797. 705
Wang, T.L., Uauy, C., Robson, F. and Till, B. (2012b) TILLING in extremis. Plant Biotechnology 706 Journal 10, 761-772. 707
Webb, A., Cottage, A., Wood, T., Khamassi, K., Hobbs, D., Gostkiewicz, K., White, M., Khazaei, H., 708 Ali, M., Street, D., Duc, G., Stoddard, F.L., Maalouf, F., Ogbonnaya, F.C., Link, W., Thomas, 709 J. and O'Sullivan, D.M. (2016) A SNP-based consensus genetic map for synteny-based trait 710 targeting in faba bean (Vicia faba L.). Plant Biotechnol J 14, 177-185. 711
Yang, T., Bao, S.Y., Ford, R., Jia, T.J., Guan, J.P., He, Y.H., Sun, X.L., Jiang, J.Y., Hao, J.J., Zhang, 712 X.Y. and Zong, X.X. (2012) High-throughput novel microsatellite marker of faba bean via 713 next generation sequencing. Bmc Genomics 13. 714
Page 21 of 21
Young, N.D., Debelle, F., Oldroyd, G.E., Geurts, R., Cannon, S.B., Udvardi, M.K., Benedito, V.A., 715 Mayer, K.F., Gouzy, J., Schoof, H., Van de Peer, Y., Proost, S., Cook, D.R., Meyers, B.C., 716 Spannagl, M., Cheung, F., De Mita, S., Krishnakumar, V., Gundlach, H., Zhou, S., Mudge, J., 717 Bharti, A.K., Murray, J.D., Naoumkina, M.A., Rosen, B., Silverstein, K.A., Tang, H., 718 Rombauts, S., Zhao, P.X., Zhou, P., Barbe, V., Bardou, P., Bechner, M., Bellec, A., Berger, 719 A., Berges, H., Bidwell, S., Bisseling, T., Choisne, N., Couloux, A., Denny, R., Deshpande, 720 S., Dai, X., Doyle, J.J., Dudez, A.M., Farmer, A.D., Fouteau, S., Franken, C., Gibelin, C., 721 Gish, J., Goldstein, S., Gonzalez, A.J., Green, P.J., Hallab, A., Hartog, M., Hua, A., 722 Humphray, S.J., Jeong, D.H., Jing, Y., Jocker, A., Kenton, S.M., Kim, D.J., Klee, K., Lai, H., 723 Lang, C., Lin, S., Macmil, S.L., Magdelenat, G., Matthews, L., McCorrison, J., Monaghan, 724 E.L., Mun, J.H., Najar, F.Z., Nicholson, C., Noirot, C., O'Bleness, M., Paule, C.R., Poulain, 725 J., Prion, F., Qin, B., Qu, C., Retzel, E.F., Riddle, C., Sallet, E., Samain, S., Samson, N., 726 Sanders, I., Saurat, O., Scarpelli, C., Schiex, T., Segurens, B., Severin, A.J., Sherrier, D.J., 727 Shi, R., Sims, S., Singer, S.R., Sinharoy, S., Sterck, L., Viollet, A., Wang, B.B., Wang, K., 728 Wang, M., Wang, X., Warfsmann, J., Weissenbach, J., White, D.D., White, J.D., Wiley, G.B., 729 Wincker, P., Xing, Y., Yang, L., Yao, Z., Ying, F., Zhai, J., Zhou, L., Zuber, A., Denarie, J., 730 Dixon, R.A., May, G.D., Schwartz, D.C., Rogers, J., Quetier, F., Town, C.D. and Roe, B.A. 731 (2011) The Medicago genome provides insight into the evolution of rhizobial symbioses. 732 Nature 480, 520-524. 733
Zeid, M., Mitchell, S., Link, W., Carter, M., Nawar, A., Fulton, T. and Kresovich, S. (2009) Simple 734 sequence repeats (SSRs) in faba bean: new loci from Orobanche-resistant cultivar ‘Giza 402’. 735 Plant Breeding 128, 149-155. 736
Zeid, M., Schon, C.C. and Link, W. (2003) Genetic diversity in recent elite faba bean lines using 737 AFLP markers. Theor Appl Genet 107, 1304-1314. 738
Zhang, H.M., Wheeler, S., Xia, X., Radchuk, R., Weber, H., Offler, C.E. and Patrick, J.W. (2015) 739 Differential transcriptional networks associated with key phases of ingrowth wall construction 740 in trans-differentiating epidermal transfer cells of Vicia faba cotyledons. BMC plant biology 741 15, 103. 742
Zong, X.X., Liu, X.J., Guan, J.P., Wang, S.M., Liu, Q.C., Paull, J.G. and Redden, R. (2009) 743 Molecular variation among Chinese and global winter faba bean germplasm. Theor Appl 744 Genet 118, 971-978. 745