Adhesion-Based Cell Sorter w ith Antibody-immobilized Fun ctionalized-Paryene Surface Author : Junichi Miwa, Yuji Suzuki and Nobuhide asagi Department of Mechanical Engineering,The University of Tokyo Hongo 7-3-1,Bunkyo-ku, Tokyo 113-8656,Japan Reporter: Wun-Hao Wu ( Wun-Hao Wu ( 吳吳吳 吳吳吳 ) ) 11/21, 2007
10
Embed
Adhesion-Based Cell Sorter with Antibody-immobilized Functionalized-Paryene Surface
Adhesion-Based Cell Sorter with Antibody-immobilized Functionalized-Paryene Surface. Author : Junichi Miwa, Yuji Suzuki and Nobuhide asagi Department of Mechanical Engineering,The University of Tokyo Hongo 7-3-1,Bunkyo-ku, Tokyo 113-8656,Japan Reporter: Wun-Hao Wu ( 吳文豪 ) - PowerPoint PPT Presentation
Welcome message from author
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
Adhesion-Based Cell Sorter with Antibody-immobilized Functionalized-Paryene
Surface
Author: Junichi Miwa, Yuji Suzuki and Nobuhide asagi Department of Mechanical Engineering,The University of TokyoHongo 7-3-1,Bunkyo-ku, Tokyo 113-8656,Japan
1. Conjugating NHS-LC-LC-biotin to surface amines.
2. NHS-LC-LC-biotin is dissolved into dimethylsulfoxide.
3. pouring into bicine buffered saline(pH 8).
4. Biotin solution is introduced into micro column and incubated for one hour at 30 .℃
5. Streptavidin and biotin-conjugated CD31 antibody solution is,each of them dissolved into PBS(pH 7.4).
Fabrication
result
Time-averaged HUVEC velocity in microcolumns with various surface antibody immobilizations.
HUVEC
Antibody
HUVEC and HL60 are mixed at 5 ×105 cells/mL total concentration, at the ratio of 1:1.
The flow is driven by a syringe pump at the total flow rate of 1.2 μL/min, with the volume ratio between the main inlet and two hydeodynamic focousing buffer inlets being 2:1:1. the corresponding bulk mean flow velocity in the cell sorting column is 1 mm/s
Conclusion
Label-free sorting. Higher throughput In the future, We can identify for some our diff
erent bodies cells, such as stem cells or monocyte. It can help us to recognize how it work.