Activities in the 1st reporting period and tasks for the 2nd period Szeged – Timişoara axis for the safe food and feed SZETISA1 HURO/0901/147/2.2.2. Erdei.

Activities in the 1st reporting period and tasks for the 2nd periodSzeged – Timişoara axis for the safe food and feed

SZETISA1HURO/0901/147/2.2.2.

Erdei LászlóDepartment of Plant Biology

University of Szeged

1st Progress Report Meeting Timişoara, 30-31 May 2011

Reporting period: 01.02. 2011 – 31.05. 2011

Main events since the Project Inaugural MeetingSzeged, 2-3 February, 2011

Subsidy Contract signed – Budapest, 22nd April 2011Szeged, 29th April 2011

ERDF Contract – en route (not yet signed)

Report deadlines:

PR- Partner Report: 15 days to VÁTI and BRECO, respectively,in HU: Szécsi Jenő, Békéscsaba , Tel. 66/ 520-250

PPR – Progress Report to JTS (in HU: Kovács Gyula, Tel. 66/520-257) within 120 days (in the contract: 28. 09. 2011)

Reporting periods

Activities

0.01.11.2009-31.01.2011

1.01.02.2011- 31.05.2011.

2.01.06.2011- 30.09.2011.

3.01.10.2011- 31.01.2012.

4.01.02.2012- 31.05.2012.

A0 PreparationMM

A1 ManagementA2 Communication

M M M M M

A3 ResearchPreliminary sample collection Szeged

TimisoaraProcuration and installation of equipments Szeged, Timisoara

, Plant growing and sample collection

Szeged, Timisoara

Physiological and biochemical measurements

Szeged

Microbiological and toxin investigations TimsoaraElement analysis

SzegedClosing activities

Szeged, Timisoara

,

Time-frameBars represent the periods of activities. Locations of activities are noted under the bars.Bold M, meetings in Szeged; Italics M, meetings in Timisora.

i)The two sides of the micronutrient availability (deficiency and excess);

ii) The presence of microbial toxins due to microbial (fungal and/or bacterial) infections and their effects on the microelement accumulation (effects of toxins on channel activity);

iii) Toxins and physiological/metabolic processes;

iv) The effects of genomic background on micronutrient accumulation and distribution.

General objectives: The interactive effects of

abiotic,biotic and

genetic factors

on the performance of wheat, maize and alfalfa plants and yield components

that may influence nutrient quality and safety

1st Reporting period: 01. 02. 2011 – 31. 05. 2011 Deadline of the report according to the contract: 28. 09. 2011.

1. Project inaugural (Szeged) and progress report preparatory (Timisoara) meetings.

2. Visibility activities (press and electronic media)

3. Initialization of instrument procurements.

4. Microelement analysis of plant samples collected in summer 2010 (during the zero period).

5. Biochemical investigations on the saples of summer 2010: wheat flag leaves (defence enzymes).

2nd Reporting period: : 01. 06. 2011. – 30. 09. 2011 Deadline of the report according to the contract: 28. 01. 2012

1. Management and communication.

2. Procuration of instruments, installation, teaching.

3. Collection wheat plant parts (flag leaves, ears) from Fusarium-inoculated and control palnts, different genotypes for element and biovchemical analyses.

4. Physiological and in situ photosynthesys measurements. Determination of antioxidant enzymes.

5. Toxicology in Timisoara.

6. Progress report preparatory meeting in Szegeden.

Taks for the next reporting period (01.06.2011 – 30.09.2011

Activity Package 0 11/2009 – present Preparation activitiesPreparatory Meetings: -25.11. 2009 Szeged; 13.01.2010 Timisoara

Activity Package 1 2/2011 – 5/2012 Project management

Project preparatory meetings2; Szeged 1, Timisoara 1

Progress reports and closing report progress report 3,

closing report 1

Closing seminars2; Timisoara 1,

Szeged 1

Improved infrastrucure in the laboratories Szeged 1,Timisoara 3 instruments

Activity Package 2 2/2011 – 5/2012 Communication activities

Results - Project meetings5; Szeged 3, Timisoara 2

Results - Publications7; Szeged 5, Timisoara 2

Outputs - Number of participants in meetings

20 in each, altogether 100.

Project Booklet, 100 pcs., Project

brochure - 100pcs.

Outputs - Number of readers in newspapers N/A

Outputs - number of authors in scientific publications, number of students on courses6 authors, 300

students

Activity Package 3 02/2011 – 05/2012 Research and Development

1. Procuration of equipments 2-7/2011 Szeged, Timisoara

Procuration of equipments is planned in both localities to improve infrastucture. In Szeged, an inductively coupled plasma mass spectrometer (ICP-MS) is planned for the multielement analysis of microelements in environmnetal, food and feed plant samples in the region of mg/kg to pg/kg. In Timisora, the purchase of a Bioanalyzer, a UV-VIS Spectophotometer and a cooling centrifuge are necessary to improve molecular biological work.

2. Determination of microelements as affected by microbial infection or heavy metals presence . 7/2011 – 5/2012 Szeged, TimisoaraThese will be determined in 2 wheat cultivars, 2 corn genotypes (GM and conventional) and alfalfa cultivars of different susceptibilities. Microelements will be determined in Szeged, microbial presence and toxins in Timisoara.

3. Determination of antioxidant defence mechanisms as influenced by microbial toxins 7/2011 – 5/2012 SzegedThe catalase, superoxide dismutase, peroxidase , glutathione S-transferase activities will be determined in the same biologicical material. In addition, glutamine syntethase iso-enzymes and Rubisco levels will be determined by 2D gel chromatography. These will be carried out in Szeged.

4. Effects of genomic background on essential and toxic microelements 7/2011 – 5/2012 Szeged, TimisoaraThis subactivity is based on earlier results: we have shown out significantly different levels of Fe, Zn, Cu and Mn in wild, ancient and cultivated wheat species in the genera Triticum and Aegilops. Alfalfa (Medicago sativa) cultivars also show different phytoremediation potential for both inorganic and organic pollutants. Besides the analysis of wheat and alfalfa cultivars, investigation for their membrane-transport mechanisms and photosynthetic activities will also be carried out.

Increase laboratory infrastructure2 laboratories,

Szeged 1, Timisoara 1

Results - Determination of factors influencing food (wheat ) and feed (fodder corn and alfalfa) nutrient safety and mineral composition

3 groups, mineral factors,

toxin factors, genetic factors

Output - Equipments for mineral analysis and for molecular biological work

Szeged, 1 ICP-MS equipment; Timisoara,

3 molecular biological

equipments.

Output - Mineral composition, toxins, genetic background

3 for wheat, 3 for corn and 3 for

alfalfa: mineral composition

1, toxins 1, genetic background 1., each,

together 9

Results and Outputs

Thank you for your attention

Analysis of wheat linesresistant or sensitive for Fusarium

Some essential and toxic elements in wheat as determined by ICP