Active Transport

INTRODUCTION

METHODOLOGY

PREPARATION OF FROG SKIN BAG

PREPARATION OF FROG SKIN BAG (CONT.)

EFFECTS OF CYANIDE IN ACTIVE TRANSPORT

EFFECTS OF CYANIDE IN ACTIVE TRANSPORT (CONT.)

EFFECTS OF CYANIDE IN ACTIVE TRANSPORT (CONT.)

EFFECTS OF CYANIDE IN ACTIVE TRANSPORT (CONT.)

EFFECTS OF CYANIDE IN ACTIVE TRANSPORT (CONT.)

Ringer’s Solution

1:1 Ringer’s Solution :

NaCN

1:2 Ringer’s Solution :

NaCN

Initial Weight 3.6 g 7.13 g 8.3 g

1st Trial 3.8 g 8.37g 9.4 g

2nd Trial 3.9 g 8.16 g 9.9 g

3rd Trial 4.1 g 7.54 g 9.6 g

4th Trial 4.1 g 7.62 g 9.3

5th Trial 4.1 g 7.25 g 9.2 g

6th Trial - 7.25 g -

TABLE OF RESULTS

RATE OF DIFFUSION

Ringer’s Solution

Length of toad skin = 6 cmWidth of toad skin = 3 cm

4.1 g – 3.6 gR = 3000s____ = 0.93 x 10-5 g/cm2s

(6cm x 3cm)

RATE OF DIFFUSION

1:1 Ringer’s Solution:NaCN

Length of toad skin = 5.5 cmWidth of toad skin = 4 cm

7.25 g – 7.13 gR = 3600s____ = 0.15 x 10-5 g/cm2s

(5.5cm x 4cm)

RATE OF DIFFUSION

1:2 Ringer’s Solution:NaCN

Length of toad skin = 6.5 cmWidth of toad skin = 3.4 cm

9.2 g – 8.3 gR = 3000s____ = 1.36 x 10-5 g/cm2s

(6.5cm x 3.4cm)

ACTIVE TRANSPORT

Active transport describes what happens when a cell uses energy to transport individual molecules across the cell membrane. The liquids inside and outside of cells have different substances. Sometimes a cell has to work and use some energy to maintain a proper balance of ions and molecules.

ACTIVE TRANSPORTActive transport usually happens across the cell membrane. Proteins are positioned to cross the membrane so one part is on the inside of the cell and one part is on the outside. Only when they cross the bilayer are they able to move molecules and ions in and out of the cell. The membrane proteins are very specific.

Many times, proteins have to work against a concentration. gradient.

ACTIVE TRANSPORT

ACTIVE TRANSPORT

ACTIVE TRANSPORT

There are poisons that stop the membrane proteins from transporting their molecules. Those poisons are called inhibitors. Sometimes the proteins are destroyed and other times they are just plugged up.