www.criver.com EVERY STEP OF THE WAY AccuGENX-ID ® BacSeq Accurate identification of bacterial isolates is critical to environmental monitoring programs. Charles River’s AccuGENX-ID ® BacSeq, bacterial identification service, utilizes comparative sequencing of the 16S ribosomal RNA (rRNA) gene in bacteria, which has been proven to be the most accurate method for identifying unknown organisms. The Advantages of Genotypic Analysis For many organisms, traditional phenotypic identification is problematic, takes a great amount of time, and can result in subjective interpretation of the test results. Not all strains within a given species consistently exhibit a particular characteristic, thereby limiting phenotypic identification methods. Additionally, library databases utilized in support of phenotypic identification are limited and challenging to update. Our genotypic analysis process stems from the reference method used by taxonomists. It analyzes DNA sequence data through a conventional assembly process, which includes auto-assembly and manual review, and correction of base caller errors. The BacSeq sequencing technique is independent of growth conditions or viability of the isolate because it only needs genomic DNA from your microbe. The resulting DNA sequence of each sample submitted for identification is compared to the validated Accugenix ® library – the industry’s most comprehensive and up-to-date database for environmental isolates – and the resulting phylogenetic analysis is unparalleled in the industry. The AccuGENX-ID ® Database and Accugenix ® Validated Libraries Charles River is committed to providing the most accurate identifications to the industries we serve. This commitment requires that we continuously update our Accugenix ® validated libraries to stay current with an evolving microbial world. Since our objective is to achieve the highest percentage of species-level identifications, we compare sequence data from samples that do not result in a species-level match against a variety of publicly available sequence databases. If a newly described organism is a good match for those sample sequences and is validly named and published, our scientists proceed with our validated library entry process. MICROBIAL SOLUTIONS Advantages: • Over 100,000 bacterial samples identified each year • Industry’s highest reportable rate of 98% • Rapid results, including same- day, with a 99.6% on-time delivery rate • Validated bacterial library with the most relevant species entries for manufacturing facilities • AccuGENX-ID ® identification process is cGMP-compliant • AccuGENX-ID ® sequence- based approach is more reliable than traditional ID methodologies Click to learn more