SJB0018 Lui Min Xi Chan Yik Tung Au Tin Wai Abstract We all had persecuted by the acnes when we were in puberty. To kill the acnes, most of the people prefer to use chemical treatments; however these treatments may not be suitable for people with hypersensitive skin. The water absorbent in the chemical remedies also absorbs water and oil from the skin cells and results in dehydration of the skin. So, the potential of some natural substances on replacing the chemical acne treat- ment was studied in this project. Clear Zone test was conducted to test the susceptibility of the natural substances to acnes bacteria, Propionibacterium acnes which cause inflammation in the hair follicles. After a series of tests, it was found that some folk prescriptions shared among people or in the mass media were be misleading. Women are suggested to be critical to the beauty information they found on the Internet. Propionibacterium acnes Methodology and Results The folk prescriptions for the acnes treatment: Mung Bean Ground Apple Cider Vinegar Almond Oil Carrot Juice Tea Tree Oil Dandelion Cinnamon in Honey Coconut Oil Preparation of samples For Tea Tree Oil, Green Bean Powder, Apple Cider Vinegar, Cinnamon Honey, Coconuts Oil: Dissolving in water Disinfecting under UV For Almond Oil, Carrots Juice, Dandelion : Blending Boiling Clear Zone Test Filter paper discs were soaked with samples and placed on agar plates with the bacterial culture. Diameter of the clear zone surrounding the paper disc indicated the effectiveness of sample solu- tions in inhibiting bacterial growth in dose- dependent manner. Growth of P. acnes in vacuum Diameters of clear zones surround the paper discs soaked with different samples (Student’s t-test (n=3) *p < 0.05 ** p < 0.01 *** p < 0.005) Results Tea Tree Oil Clear zone: 29.17 mm Tea Tree Oil: 29.17 mm Control: 0 mm Carrot: 28.73 mm P. acnes were grown on a Trypticase soy agar (TSA) medium stored in a vacu- um chamber at 37˚C. Deliverables and Conclusive remarks 1. UV sensitivity test could also be another tool for study. 2. Antibacterial action of chemicals could be quantified by pour plate method. 3. Many phytochemicals with antibacterial or medicinal effects were usually organic and non-polar. To elucidate their potential antibacterial effect might need detailed procedures of extrac- tion by using various organic solvents. Tea Tree Oil Carrot Juice Dandelion Almond Oil *** *** *** *** ***