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About OMICS Group · Principal investigator: Dr Chandrakanth H.V. Associate professor CCoo-investigator: Dr B.M. Balaraj investigator: Dr B.M. Balaraj Professor & Head Dr H.S.Virupaksha
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OMICSOMICS GroupGroup InternationalInternational isis aa pioneerpioneer andand leadingleading sciencescience eventevent
chemistry chemistry –– An evaluation of An evaluation of
sodium, potassium and sodium, potassium and
chloride levels in estimation chloride levels in estimation
of time since death ( during of time since death ( during of time since death ( during of time since death ( during
the first 36hours after death the first 36hours after death
))
Principal investigator: Dr Chandrakanth H.V.Principal investigator: Dr Chandrakanth H.V.
Associate professorAssociate professor
CoCo--investigator: Dr B.M. Balaraj investigator: Dr B.M. Balaraj
Professor & HeadProfessor & HeadProfessor & HeadProfessor & Head
Dr H.S.Virupaksha Dr H.S.Virupaksha
Professor & HeadProfessor & Head
Dr T. N. Chandrasekhar Dr T. N. Chandrasekhar
Professor & Head Professor & Head
IntroductionIntroductionEstimation of time since death is a Estimation of time since death is a
paramount medicoparamount medico--legal issue in autopsylegal issue in autopsy
An appropriate range of postmortem An appropriate range of postmortem
interval can be deduced by intelligent interval can be deduced by intelligent
interpretation of various changes that interpretation of various changes that interpretation of various changes that interpretation of various changes that
show biochemical changes after death.show biochemical changes after death.
Vitreous is gained from the vitreous body and is Vitreous is gained from the vitreous body and is
accompanied by decelerated diffusion and accompanied by decelerated diffusion and
hardly contaminated even in late postmortem hardly contaminated even in late postmortem
intervals.intervals.
This study was intended to study correlation This study was intended to study correlation
between postmortem interval and vitreous between postmortem interval and vitreous
humor chemistry for sodium, potassium and humor chemistry for sodium, potassium and humor chemistry for sodium, potassium and humor chemistry for sodium, potassium and
chlorides.chlorides.
Aimed to find male tp female differences and Aimed to find male tp female differences and
differences between right and left eyes in differences between right and left eyes in
vitreous chemistryvitreous chemistry
VOLUNTEER’S CONSENT FORM
Materials & methodsMaterials & methods
�� Study was carried out in mortuary, Department Study was carried out in mortuary, Department
of Forensic medicine in association with Dept of Forensic medicine in association with Dept
of Biochemistry along with MMC&RI of Biochemistry along with MMC&RI
Forensic Medicine deptForensic Medicine dept
�� Vitreous was collected from 100 deceased Vitreous was collected from 100 deceased �� Vitreous was collected from 100 deceased Vitreous was collected from 100 deceased
subjected for autopsy.subjected for autopsy.
�� All the cases where exact time of death was All the cases where exact time of death was
known and where the time since death ranged known and where the time since death ranged
between 0 and 36 hours were included in the between 0 and 36 hours were included in the
study study
Inclusion criteriaInclusion criteria
Crystal clear fluid will be used for analysisCrystal clear fluid will be used for analysis
Exclusion criteriaExclusion criteria
�� Cases with known or suspected ocular disease, Cases with known or suspected ocular disease,
localized trauma, head injury involving orbit, localized trauma, head injury involving orbit,
burns.burns.
�� Vitreous contaminated with blood and tissue.Vitreous contaminated with blood and tissue.�� Vitreous contaminated with blood and tissue.Vitreous contaminated with blood and tissue.
�� 18 gauge needle will be inserted obliquely 18 gauge needle will be inserted obliquely
through sclera, 5mm lateral to limbus, this through sclera, 5mm lateral to limbus, this
traverse the pars plana and enter vitreous body traverse the pars plana and enter vitreous body
and gently 2ml of vitreous will be drawn into and gently 2ml of vitreous will be drawn into and gently 2ml of vitreous will be drawn into and gently 2ml of vitreous will be drawn into
10ml sterile syringe and later transferred to 10ml sterile syringe and later transferred to
borosil 5ml test tube, the same is repeated on borosil 5ml test tube, the same is repeated on
the other eyethe other eye
�� Collected Vitreous humor was centrifuged at Collected Vitreous humor was centrifuged at
3000 g and supernatant 3000 g and supernatant -- transferred to another transferred to another
test tube and sent to Laboratory for test tube and sent to Laboratory for
estimation. Care was ensured to prevent any estimation. Care was ensured to prevent any estimation. Care was ensured to prevent any estimation. Care was ensured to prevent any
contamination with blood/RBC’scontamination with blood/RBC’s
�� In the Laboratory, Potassium will be estimated In the Laboratory, Potassium will be estimated by Ion Selective Electrode Methods on AVL by Ion Selective Electrode Methods on AVL Roche Electrolyte Analyzer. Roche Electrolyte Analyzer.
�� Potassium electrodes were fitted with liquid Potassium electrodes were fitted with liquid �� Potassium electrodes were fitted with liquid Potassium electrodes were fitted with liquid ion exchange membranes that incorporate ion exchange membranes that incorporate valinomycin.valinomycin.
�� The analyzer can measure Potassium levels in The analyzer can measure Potassium levels in
the range of 1.8 to 12 mmol/litre. the range of 1.8 to 12 mmol/litre.
�� Daily Quality checks will be performed using Daily Quality checks will be performed using
level 2 and level 3 quality control sera from level 2 and level 3 quality control sera from level 2 and level 3 quality control sera from level 2 and level 3 quality control sera from
BioBio--Rad Laboratories.Rad Laboratories.
Statistical methodStatistical method
�� Data obtained was analyzed statistically using Data obtained was analyzed statistically using
SPSS version 11.0 SPSS version 11.0
�� The present research did not find a significant The present research did not find a significant
correlation between vitreous chemistry and correlation between vitreous chemistry and
postmortem interval. The differences in postmortem interval. The differences in