References: Wildanger, D. et al. “Solid Immersion Facilitates Fluorescence Microscopy with Nanometer Resolution and Sub-Ångström Emitter Localization“, Adv. Mater. 24, 309 (2012) Specs: 2-color 2D STED resolution < 30x30 nm (25x25 nm typical) 3D STED resolution < 100x100x100 nm, 80x80x90 nm typical (with easy 3D STED module) Change between objectives (water/ oil/ glycerol immersion) APD detection with highest detection sensitivity (>62% @ 680nm) Pulsed STED laser and time gating Combine with STED @595nm and RESOLFT for up to 6 superresolution channels Pulse / pixel / line / frame interleaved acquisition Abberior multicolor STED @ 775 nm Spectral properties and labels: 488 nm 594 nm 640 nm 775 nm Excitation wavelengths pulsed STED Recommended labels: Abberior STAR 520SXP, Abberior STAR 580, Abberior STAR 635P, Abberior STAR RED, ATTO 647N, ATTO 590, etc. Confocal STED RESCue mode optional Göttfert, F. et al. “Coaligned Dual-Channel STED Nanoscopy and Molecular Diffusion Analysis at 20 nm Resolution“, Biophysical J. 105, L01 (2013) Harke, B. et al. “Three-Dimensional Nanoscopy of Colloidal Crystals“, Nano Lett. 8, 1309 (2008) Berning, S. et al. “Nanoscopy in a Living Mouse Brain“, Science 335, 551 (2012) 561nm 520 nm Aberration corrections for 2D and 3D STED