www.abcam.com ab108758 Parainfluenza Virus I ELISA Kit Instructions for Use For the qualitative measurement of Huma class antibodies to Parainfluenza virus (ty 2 and 3) in serum or plasma. This product is for research use only and intended for in vitro diagnostic use. IgG an IgG- ypes 1, d is not
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ab108758 GenWay Parainfluenza Virus IgG ELISA Kit · 2011-07-12 · ab108758 Parainfluenza Virus IgG ELISA Kit 4 years, as determined by seroepidemiological studies. Antibodies against
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www.abcam.com
ab108758
Parainfluenza Virus IgG
ELISA Kit
Instructions for Use
For the qualitative measurement of Human IgGclass antibodies to Parainfluenza virus (types 1, 2 and 3) in serum or plasma. This product is for research use only and is not intended for in vitro diagnostic use.
Parainfluenza Virus IgG
For the qualitative measurement of Human IgG-class antibodies to Parainfluenza virus (types 1,
This product is for research use only and is not
ab108758 Parainfluenza Virus IgG ELISA Kit
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ab108758 Parainfluenza Virus IgG ELISA Kit
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Table of Contents
1. Introduction 3
2. Assay Summary 5
3. Kit Contents 6
4. Storage and Handling 7
5. Additional Materials Required 7
6. Preparation of Reagents 8
7. Preparation and Collection of Specimen 10
8. Assay Method 11
9. Data Analysis 15
10. Limitations 17
11. Specificity 18
12. Troubleshooting 19
ab108758 Parainfluenza Virus IgG ELISA Kit
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1. Introduction
ab108758, Parainfluenza Virus IgG ELISA Kit is intended for the
qualitative determination of IgG class antibodies against
Parainfluenza Virus (Types 1, 2, 3) IgG in Human serum or plasma
(citrate).
Parainfluenza viruses are important viral pathogens causing upper
and lower respiratory infections in adults and children. The viruses
belong to the family of Paramyxoviridae. They are enveloped single-
stranded RNA viruses with spherical or pleomorphic shape.
Parainfluenza viruses are relatively large viruses of about 150-
300 nm in diameter. On the basis of antigenic differences they are
divided into four subtypes, of which type 4 is divided into two
subtypes. Parainfluenza virus 1 and 3 belong to the paramyxovirus
genus. Parainfluenza virus 2, 4a and 4b belong to the rubella virus
genus along with mumps. The virus is ubiquitous; infections occur as
epidemics as well as sporadically. Parainfluenza viruses are
sensitive to detergents and heat but can remain viable on surfaces
for up to 10 hours. Transmission occurs via droplets, aerosols and
fomites (viruses survive on surfaces). Parainfluenza virus infections
are primarily childhood diseases, the highest age-specific attack
rates for croup occur in children below the age of 3 years. Type 3
infections occur earliest and most frequently, so that ~50% of
children are infected during the first year of life and almost all by 6
ab108758 Parainfluenza Virus IgG ELISA Kit
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years, as determined by seroepidemiological studies. Antibodies
against types 1 and 2 are acquired less rapidly but 80% of children
have antibodies by 10 years of age. Type 4 viruses induce few
clinical illnesses but infections are common, 70 - 80% of children
have antibodies by 10 years of age. In all age groups, the incubation
period appears to be five to six days. Croup or
laryngotracheobronchitis is the commonest clinical manifestation of
infection. Parainfluenza viruses are found uncommonly associated
with other respiratory tract infections in children such as
tracheobronchitis, bronchiolitis, and bronchopneumonia.
Occasionally, a mild non-specific illness is seen after parainfluenza
virus infection. In adults, the virus is usually limited to causing
inflammation in the upper parts of the respiratory tract.
ab108758 Parainfluenza Virus IgG ELISA Kit
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2. Assay Summary
ab108758 is based on the principle of a solid phase enzyme-linked immunosorbent assay. The assay system utilizes antibodies directed against distinct antigenic determinants on the Parainfluenza Viruses (Types 1, 2 and 3).
The microtiter wells are precoated with Parainfluenza Virus antigens to bind corresponding antibodies of the specimen.
The test sample is allowed to react with the antigens. The wells are washed to remove all unbound sample material. Horseradish peroxidase (HRP) labelled anti-Human IgG conjugate is added. This conjugate binds to the captured parainfluenza virus-specific antibodies.
The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate, which gives a blue reaction product.
The colour development is stopped by the addition of sulphuric acid changing the colour to yellow. The colour intensity of the test sample is proportional to the amount of parainfluenza virus-specific IgG antibodies in the specimen. Absorbance is measured spectrophotometrically at 450 nm.