Aalborg Universitet Butyric acid fermentation with in-situ products separation Baroi, George Nabin; Skiadas, Ioannis; Westermann, Peter; Gavala, Hariklia N. Publication date: 2014 Document Version Accepted author manuscript, peer reviewed version Link to publication from Aalborg University Citation for published version (APA): Baroi, G. N., Skiadas, I., Westermann, P., & Gavala, H. N. (2014). Butyric acid fermentation with in-situ products separation. Poster presented at Tomorrow’s biorefineries in Europe, Brussels, Belgium. General rights Copyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright owners and it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights. ? Users may download and print one copy of any publication from the public portal for the purpose of private study or research. ? You may not further distribute the material or use it for any profit-making activity or commercial gain ? You may freely distribute the URL identifying the publication in the public portal ? Take down policy If you believe that this document breaches copyright please contact us at [email protected] providing details, and we will remove access to the work immediately and investigate your claim. Downloaded from vbn.aau.dk on: December 09, 2020
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Aalborg Universitet
Butyric acid fermentation with in-situ products separation
Baroi, George Nabin; Skiadas, Ioannis; Westermann, Peter; Gavala, Hariklia N.
Publication date:2014
Document VersionAccepted author manuscript, peer reviewed version
Link to publication from Aalborg University
Citation for published version (APA):Baroi, G. N., Skiadas, I., Westermann, P., & Gavala, H. N. (2014). Butyric acid fermentation with in-situ productsseparation. Poster presented at Tomorrow’s biorefineries in Europe, Brussels, Belgium.
General rightsCopyright and moral rights for the publications made accessible in the public portal are retained by the authors and/or other copyright ownersand it is a condition of accessing publications that users recognise and abide by the legal requirements associated with these rights.
? Users may download and print one copy of any publication from the public portal for the purpose of private study or research. ? You may not further distribute the material or use it for any profit-making activity or commercial gain ? You may freely distribute the URL identifying the publication in the public portal ?
Take down policyIf you believe that this document breaches copyright please contact us at [email protected] providing details, and we will remove access tothe work immediately and investigate your claim.
Butyric acid fermentation with in-situ products separation
Baroi G.N 1, Skiadas I.V. 1, Westermann P. 1, Gavala H.N1. 1Aalborg University Copenhagen (AAU-Cph), Department of Biotechnology, Chemistry and Environmental Engineering, A C Meyers Vænge 15, DK 2450 Copenhagen SV Denmark.
Background Butyric acid and its esters are used as food supplements, as artificial flavours, as solvent and even its potential beneficial effects in intestinal and extra intestinal diseases and sickle cell disease have been reported. Another potential use of butyric acid is to produce butanol via catalytic hydrogenation. The demand of the butyric acid is approximately 50,000 ton/year. The main bottlenecks of commercialization of biological production of acids ( in overall) are: (1) utilization of cheap feed stocks (2) Proper strain selection &/or improvement (3) Process development for higher productivity (4) Downstream processing The first three aspects were successfully considered for biological butyric acid production from pretreated and hydrolysed wheat straw (PHWS) by C.tyrobutyricum. Continuous fermentation and in-situ separation of butyric acid increased C6 and C5 consumption and butyric acid production rates compared to batch fermentation without in-situ products removal. The process efficiency was verified in pilot-scale as well. However, further improvement of downstream process required.
Acknowledgement
The authors wish to thank the Commission of the European Communities for the financial support of this work under SUPRABIO project (FP7-cooperationproject no 241640).
0
10
20
30
40
50
60
70
80
glucose xylose butyric acid acetic acid
Rat
e (
g/d
ay)
Remain in the fermentor
Product extracted by REED
metabolized
Acid from Feedflow extracted by REED
In-s
itu
pro
du
ct
sep
arat
ion
at
Pilo
t sc
ale
(20
L)
Pretreatment Enzymatic hydrolysis
Strain selection and development
Process development
In-situ product separation at lab scale
(3L)
Process model of butyric acid production from wheat straw
Conclusion • Fermentation coupled with REED in-situ separation of PHWS with the adapted C. tyrobutyricum gave a higher
(28 to 58%) butyric acid yield compared to synthetic medium fermentation. • Continuous fermentation exhibited much higher (>600% for 60% PHWS) sugars consumption rates compared
to batch fermentations • With REED in-situ acid seperation results higher sugars consumption rate and C4 production rates (> 46%) • Fermentation coupled with REED in-situ separation of 100% PHWS continued unhindered with just urea and
K2HPO4 added reaching a productivity of 1,31 g/l/h butyric acid production and 97% sugars utilization
Schematic diagram of the experimental setup Rates of synthetic medium ( mixed glucose and xylose) based fermentation with and without in-situ acids separation by REED at HRT 1 day
Rates of PHWS based fermentation with and without in-situ acids separation by REED at HRT 1 day
Continuous fermenation of 100% PHWS with REED
0.0
0.5
1.0
1.5
2.0
2.5
A B C
With REED Without REEDCo
nsu
mp
tio
n a
nd
pro
du
ctio
n r
ate
(g
/L/h
)
Glucose Xylose Acetic acid Butyric acid Hydrogen
Glucose and Xylose concentrations (g/L) for A (38 , 22), B (54, 34) and C (38, 22)