A8-023287 Tamas Nagy, DVM, PhD Department of Pathology College of Veterinary Medicine University of Georgia Presented at SEVPAC 2008 – Permission granted for use on SEVPAC website
Dec 16, 2015
A8-023287
Tamas Nagy, DVM, PhD
Department of PathologyCollege of Veterinary Medicine
University of Georgia
Presented at SEVPAC 2008 – Permission granted for use on
SEVPAC website only
A8-023287
• Signalment and history– 3-month-old female IL-10 knock-out mouse– Kept in an individually ventilated microisolator
cage with one other female and one male– Gave birth 7 days ago, but all the offspring
died– No previous reproductive problems– Developed rectal prolapse 24 hours prior to
euthanasia
Presented at SEVPAC 2008 – Permission granted for use on
SEVPAC website only
A8-023287
• Gross pathology– Rectal prolapse– Gas-filled and moderately dilated proximal
colon– Moderately thickened proximal colonic wall
Presented at SEVPAC 2008 – Permission granted for use on
SEVPAC website only
A8-023287
Image courtesy of Dr. R. McManamonPresented at SEVPAC 2008 – Permission granted for use on
SEVPAC website only
A8-023287
Presented at SEVPAC 2008 – Permission granted for use on
SEVPAC website only
A8-023287
Presented at SEVPAC 2008 – Permission granted for use on
SEVPAC website only
A8-023287
Presented at SEVPAC 2008 – Permission granted for use on
SEVPAC website only
A8-023287
Presented at SEVPAC 2008 – Permission granted for use on
SEVPAC website only
A8-023287
Presented at SEVPAC 2008 – Permission granted for use on
SEVPAC website only
A8-023287
Presented at SEVPAC 2008 – Permission granted for use on
SEVPAC website only
A8-023287
Presented at SEVPAC 2008 – Permission granted for use on
SEVPAC website only
A8-023287
Presented at SEVPAC 2008 – Permission granted for use on
SEVPAC website only
A8-023287
• Morphologic diagnosis– Colon: chronic, moderate, granulomatous
colitis– Rectum: Rectal prolapse with secondary
acute edema and serocellular crust formation
Presented at SEVPAC 2008 – Permission granted for use on
SEVPAC website only
A8-023287• Discussion
– IL-10 (interleukin 10)• Member of the cytokine family (involved in
regulation of T lymphocyte activity)
• Produced by T regulatory lymphocytes, TH2 lymphocytes, and dendritic cells
• Important mediator in TH2-biased immunologic response – diffuse (lepromatous) granulomas
– Johne’s disease– Human leprosy– Crohn’s disease
Presented at SEVPAC 2008 – Permission granted for use on
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A8-023287• Discussion – continued
– IL-10 knock-out (IL-10 KO) mouse• Created in 1993• Original report – IL-10 KO phenotype
– Chronic enterocolitis in mice housed under conventional conditions
– Colitis affecting only the proximal colon in mice housed under SPF conditions
• Model for human idiopathic inflammatory bowel diseases?
• Real cause: normal resident enteric bacteria especially in combination with Helicobacter hepaticus
Presented at SEVPAC 2008 – Permission granted for use on
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A8-023287• Differential diagnoses
– Citrobacter rodentium infection• Young mice or certain strains of mice affected (e.g.
FVB)• Sticky, unformed feces• Rectal prolapse often occurs• Brush border of the mucosa of the affected bowel
is carpeted with attached cocco-bacilli• Descending colon is affected• Hyperplastic colonic mucosa with excessive
numbers of goblet cells and leukocytic inflammation in the lamina propria
Presented at SEVPAC 2008 – Permission granted for use on
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A8-023287• Differential diagnoses – continued
– Coliform typhlocolitis• Atypical, non-lactose fermenting E. coli• Intestinal lesions only occur in triple deficient mice
(devoid of T, B, and NK cells)• Segmental colonic hyperplasia with erosions and
mononuclear inflammation
– Helicobacter sp. induced proliferative typhlocolitis
• Immunodeficient (SCID & nude) mice
Presented at SEVPAC 2008 – Permission granted for use on
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A8-023287• Differential diagnoses – continued
– Enterotropic MHV• Neonatal mice in naïve mouse colonies• Immunodeficient (SCID) mice• Attenuated villi with syncytia formation (balloon
cells)
Presented at SEVPAC 2008 – Permission granted for use on
SEVPAC website only