A universal nanoparticle cell- secretion capture assay for the study of HIV-1 infected tissues. A new tool to identify cytokine secreting cells Wendy Fitzgerald and Jean-Charles Grivel The Eunice Kennedy-Shriver National Institute of Child Health and Human Development, Bethesda, MD.
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A universal nanoparticle cell-secretion capture assay for the study of HIV-1 infected tissues. A new tool to identify cytokine secreting cells Wendy Fitzgerald.
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A universal nanoparticle cell-secretion capture assay for the study of HIV-1 infected
tissues.
A new tool to identify cytokine secreting cells
Wendy Fitzgerald and Jean-Charles Grivel
The Eunice Kennedy-Shriver National Institute of Child Health and Human Development, Bethesda, MD.
Identification of secreting cells
ELISPOT
Intracellular cytokine staining
Exocytosis assay
These assays have flaws:
They kill the cells being studied.
Cell fixation and permeabilization interfere with downstream molecular work.
They do not distinguish between pre-stored cytokines and secreted cytokine.
Elispot, doesn’t provide information on cell phenotype.
Exocytosis is limited to certain cell types and doesn’t reveal the nature of the cytokine secreted.
The ideal assay:
Captures the secreted cytokine before it diffuses away from the producing cells.
Is compatible with cell isolation and culture.
Some assays have been developed:
Agarose cell encapsulation (One cell systems) Requires dedicated instrumentation to encapsulate the cells