Murphy & Courtneidge (2011) Nature Reviews Molecular Cell Biology 1 Legend 0. No value (7) 1. Normal Colon (5) 2. Cecum Adenocarcinoma (17) 3. Colon Adenocarcinoma (41) 4. Colon Mucinous Adenocarcinoma (13) 5. Rectal Adenocarcinoma (8) 6. Rectal Mucinous Adenocarcinoma (4) 7. Rectosigmoid Adenocarcinoma (10) Oncomine database: SATB2 expression Normal vs. Cancer
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Murphy & Courtneidge (2011) Nature Reviews Molecular Cell Biology
1
Legend0. No value (7)1. Normal Colon (5)2. Cecum Adenocarcinoma (17)3. Colon Adenocarcinoma (41)4. Colon Mucinous Adenocarcinoma (13)5. Rectal Adenocarcinoma (8)6. Rectal Mucinous Adenocarcinoma (4)7. Rectosigmoid Adenocarcinoma (10)
Oncomine database: SATB2 expression
Normal vs. Cancer
FLAG
FLAG-S
ATB2
0
50
100
150
200
250
300
350
400
*
Nu
mb
er o
f in
vad
ed c
ells
/fie
ld
B
FLAG
FLAG-SATB2
Figure 1
FLAG
Cortactin
Phalloidin
Merge
FLAG-SATB2
Cortactin
Phalloidin
Merge
FLAG
FLAG-S
ATB2
0
10
20
30
40
50
60
70
*
% o
f ce
lls w
ith
inva
do
po
dia
C
Cortactin
FITC-gelatin
D
HCT116
GFP GFP
SATB2
CW-2
GFP GFP
SATB2
COLO 320
GFP GFP
SATB2
Palladin
β-actin
GFP GFP-SATB20
0.2
0.4
0.6
0.8
1
1.2
Rel
ativ
e m
RN
A le
vel o
f P
alla
din
A B
GFPGFP-
SATB2
HCT116
GFP
kDa
150
110
50
30
SATB2
β-actin
C110
150
97
200
kDa
Figure 2
116
66
L. Jin, 2011
HCT116
DLD-1
COLO
205
COLO
320
SW 6
20
CW-2
CACO-2
Palladin
α-tubulin
CRC cells:
HCT116
shRNA: Ctrl
PALL
D #
1 PALL
D #
2
Palladin
β-actin
Figure 3
A B
C
kDa
150
97116
200150
97
200
6666
shCtrl shPALLD #1 shPALLD #2
0 hr
12 hr
24 hr
36 hr0 12 24 36
0
10
20
30
40
50
60
70
80
90
* ***
shCtrl
shPALLD #1
shPALLD #2
Time (h)
Mig
rati
ng
dis
tan
ce(a
rbit
rary
un
it)
A
Cortactin
Phalloidin
Merge
shCtrl shPALLD #1 shPALLD #2
Figure 4
shCtrl shPALLD #1 shPALLD #20
10
20
30
40
50
60
70
* *
% o
f ce
lls w
ith
inva
do
po
dia
B
FLA
GFL
AG.S
ATB2/
GFP FL
AG.S
ATB2/
GFP
-pal
ladi
n
PALLD
GFP
Actin
97
150200
kDa
30
97
116
Figure 6
A
FLAG.SATB2/GFP
FLAG.SATB2/GFP-palladin
0
10
20
30
40
50
60
70
*
% o
f ce
lls w
ith
inva
do
po
dia
FLAG.SATB2FLAG.SATB2/GFP-palladinB
FLAG.SATB2 FLAG.SATB2/GFP-palladin
0
50
100
150
200
250
300
350 *
Nu
mb
er
of
invad
ed
cel
ls/f
ield
GFP Cortactin Merge
FLAG.SATB2/GFP-palladin (wt)D
SATB2 Palladin
ECM degradation
Migration signaling
Cytoskeletal dynamics
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