A new A new Ceratocystis Ceratocystis species from species from wounds made by elephants on wounds made by elephants on trees in the Kruger National Park trees in the Kruger National Park KAMGAN NKUEKAM GILBERT KAMGAN NKUEKAM GILBERT J. Roux & J. Roux & M.J. Wingfield M.J. Wingfield http://fabinet.up.ac.za http://fabinet.up.ac.za
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A new Ceratocystis species from wounds made by - SANParks
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A new A new CeratocystisCeratocystis species from species from wounds made by elephants on wounds made by elephants on
trees in the Kruger National Parktrees in the Kruger National Park
Some edible for humans, others toxicSome edible for humans, others toxic
Grow on a wide range of substrates Grow on a wide range of substrates egeg. Bread, dung, plants, human . Bread, dung, plants, human skin etc.skin etc.
FUNGIFUNGI
FUNGI CAN CAUSE DEVASTATING FUNGI CAN CAUSE DEVASTATING DISEASES OF AGRICULTURAL CROPS DISEASES OF AGRICULTURAL CROPS
AND TREESAND TREES
CastaneaCastanea dentatadentata -- 50% 50% of forests in Eastern of forests in Eastern
U.S.AU.S.A
!!Estimated thousand million trees in 1900Estimated thousand million trees in 1900!!1963 only ~1801963 only ~180
USDA Forest Service USDA Forest Service -- NortheasternNortheastern Area Archives, USDA Forest Service, Area Archives, USDA Forest Service, wwww..forestryimagesforestryimages.org.org
!! Infects wounds on trees Infects wounds on trees
!! Vectored by insectsVectored by insects
!! Include many important pathogensInclude many important pathogens
!! Broad host rangeBroad host range
!! Wide geographical rangeWide geographical range
CERATOCYSTISCERATOCYSTIS SPECIESSPECIES
!! Oak wiltOak wilt
"" 83% of Oak death between 1960 83% of Oak death between 1960 �� 1971 1971
"" The fungus is transmitted below ground through The fungus is transmitted below ground through
root graftroot graft
"" Above ground it is transmitted by Above ground it is transmitted by NitidulidsNitidulids
"" Native in the USA Native in the USA �� Never been reported from otherNever been reported from other
!!During recent surveys of fungi infecting woundsDuring recent surveys of fungi infecting wounds
created by elephants in the Kruger National Parkcreated by elephants in the Kruger National Park
CeratocystisCeratocystis sppspp. was found to be very common on. was found to be very common on
these woundsthese wounds
!!Very little is known about the biodiversity andVery little is known about the biodiversity and
pathogenicitypathogenicity of of CeratocystisCeratocystis sppspp. on native trees . on native trees
in South Africa in South Africa
INTRODUCTIONINTRODUCTION
AIMSAIMS
!!Identify and describe a new species of Identify and describe a new species of CeratocystisCeratocystis collected during the surveyscollected during the surveys
!!Test the ability of the fungus to cause disease on its Test the ability of the fungus to cause disease on its hostshosts
MATERIALS AND METHODSMATERIALS AND METHODSCOLLECTION OF SAMPLESCOLLECTION OF SAMPLES
!!Wounds caused by elephants Wounds caused by elephants
DNA ExtractionDNA Extraction& Sequencing& Sequencing