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A-Level Biology: Summer work
Because of the extended holiday period, students are expected to complete 20 hours of work in order to prepare
for A-level Biology next year. Please complete the following work.
1. Our usual summer worksheet which contains 2 pages of questions (see pages 2-3). This is to be completed
on a separate sheet of paper (written or typed) and handed in during your first Biology lesson in September.
2. A list of all the keywords from Terms 1 & 2. You must research these key terms and come up with a
definition for them. During the course, you can tick them off as each one comes up. See pages 4-9.
3. Exam question pack which contains data analysis, data interpretation, and graph work questions. These are
the questions that a lot of students can struggle with at A-level, and it will be beneficial to familiarise
yourselves with these types of question early. See pages 10-40. The mark scheme for these is attached as
well, so you can self-mark your answers. See pages 41-56. Evidence that these have been completed must be
shown in September.
4. Lastly, links to 6 scientific novels which have been highly recommended by various teachers appear below.
These will broaden your minds to Biology beyond the exam specification and will give you a deeper
appreciation for Science. The expectation is that you read at least two of these novels. Audible has made a
few of these free to download during these difficult times.
https://www.amazon.co.uk/First-Cell-Human-Pursuing-
Cancer/dp/B07YYGQL4B/ref=sr_1_1?dchild=1&keywords=the+first+cell&qid=1586173757&s=books&sr=1-1
https://www.amazon.co.uk/Napoleons-Buttons-Penny-Couteur-
ebook/dp/B001NQGN24/ref=sr_1_1?dchild=1&keywords=Napoleons+buttons&qid=1586173806&s=books&sr=1-1
https://www.amazon.co.uk/Gulp-Adventures-Alimentary-Mary-Roach-
ebook/dp/B07ZWK911Z/ref=sr_1_1?dchild=1&keywords=gulp&qid=1586173771&s=books&sr=1-1
https://www.amazon.co.uk/Violinists-Thumb-extraordinary-stories-written-
ebook/dp/B008H1SIVM/ref=sr_1_1?dchild=1&keywords=the+violinists+thumb&qid=1586173823&sr=8-1
https://www.amazon.co.uk/Origin-Species-Sabina-
Radeva/dp/0141388501/ref=sr_1_1?crid=1BRQHO0RH2YTL&dchild=1&keywords=the+origin+of+species&qid=158
6173787&s=books&sprefix=the+ori%2Caps%2C171&sr=1-1
https://www.amazon.co.uk/Frogs-Flies-Dandelions-Making-
Species/dp/0198503938/ref=sr_1_1?dchild=1&keywords=Frogs+flies+and+dandelions&qid=1586173842&sr=8-1
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2 PTO
A-Level Biology
The following sets of twenty questions encompass the theory you will cover in the first two Biology topics
in year 12. Answer in full sentences on lined paper and bring along to you first Biology lesson.
Biological molecules
1. Define metabolism (2 marks)
2. Name the bonding found in metals (1 mark)
3. How do you test for reducing sugars? (2 marks)
4. What two monosaccharide's join to form lactose? (2 marks)
5. Describe the test for starch (2 marks)
6. What is the main role of cellulose? (1 mark)
7. What are the two main groups of lipids? (2 marks)
8. Draw the structure of an amino acid (1 mark)
9. What are phospholipids made up of? (2 marks)
10. Name 2 properties of water (2 marks)
11. What are the 4 structures of protein molecules? (4 marks)
12. What is a condensation reaction? (1 mark)
13. What bond forms to form dipeptides? (1 mark)
14. What is a hydrolysis reaction? (1 mark)
15. True or false: Glycogen is insoluble (1 mark)
16. What are the bonds found in starch molecules? (1 mark)
17. If glucose joins to glucose then what is formed? (1 mark)
18. Describe the structure of ATP? (3 marks)
19. What is a polar molecule? (2 marks)
20. What colour should the solution go if reducing sugars are present? (1 mark)
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3 PTO
Cell structure
1. How do you calculate magnification? (3 marks)
2. What is homogenation? (1 mark)
3. Name two advantages of using an electron microscope (2 marks)
4. Name 2 limitations of using a Transmission Electron Microscope (TEM) (2 marks)
5. What do you need to calibrate an eyepiece graticule? (1 mark)
6. True or false: Eukaryotic cells contain membrane bound organelles. (1 mark)
7. What are cristae? (1 mark)
8. What 3 parts make up chloroplasts (3 marks)
9. What are the 2 parts of the endoplasmic reticulum? (2 marks)
10. Name the 2 types of ribosomes (2 marks)
11. Name an organ system (1 mark)
12. State the role of the capsule in bacterial cells (1 mark)
13. What are prokaryotic cell walls made of? (1 mark)
14. What is the definition of a tissue (1 mark)
15. True or false: viruses are larger than bacteria (1 mark)
16. Name a plant organ (1 mark)
17. Name the chemical that is used to make the cell wall of fungi (1 mark)
18. What is the role of attachment proteins in viruses (1 mark)
19. Describe epithelial tissues (2 marks)
20. What is a tonoplast? (1 mark)
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Term 1 & 2 Key Words Biological Molecules
Covalent Bond
Ionic Bond
Hydrogen Bond
Polar Molecule
Monomer
Polymer
Polymerisation
Condensation
Hydrolysis
Metabolism
Mole
Molar (M) Solution
Carbohydrate
Monosaccharide
Organic Molecule
Disaccharide
Polysaccharide
Hexose sugar
Glucose
Reducing Sugar
Reducing sugar test
Benedict’s reagent
Glycosidic bond
Non-reducing sugar
Non-reducing sugar test
Starch
Glycogen
Cellulose
Alpha glucose
Beta glucose
Lipid
Triglyceride
Fatty acid
Glycerol
Saturated fatty acid
Mono-unsaturated fatty acid
Poly-unsaturated fatty acid
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Phospholipid
Hydrophilic
Hydrophobic
Emulsion test
Protein
Amino acid
Amino group
Carboxyl group
R-group
Peptide bond
Polypeptide
Primary protein structure
Secondary protein structure
Tertiary protein structure
Disulfide bridge
Quaternary protein structure
Biuret test
Enzymes
Enzyme
Activation energy
Active Site
Substrate
Enzyme-substrate complex
Complimentary
Specific
Induced fit
Lock and key
Rate of reaction
Kinetic Energy
pH
Inhibitor
Competitive inhibitor
Non-competitive inhibitor
DNA
DNA
Nucleotide
Polynucleotide
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Phosphodiester bond
Organic base
RNA
Complimentary base pairing
Double helix
Semiconservative replication
DNA helicase
DNA polymerase
ATP (Adenosine triphosphate)
ADP
ATP Synthase
ATP Hydrolase
Polymerases
Gas Exchange
Surface area: volume ratio
Exchange surface
Concentration gradient
Tracheae
Tracheoles
Spiracle
Gill
Gill filaments
Gill lamellae
Countercurrent flow
Stomata
Guard cells
Spongy mesophyll
Xerophyte
Lungs
Ventilation
Trachea
Bronchi
Bronchioles
Alveoli
Inspiration
Expiration
Diaphragm
Rib cage
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Intercostal muscles
Cell Structure
Magnification
Resolution
Micrometer
Nanometer
Light Microscope
Electron microscope
Scanning Electron Microscope (SEM)
Transmission electron microscope (TEM)
Cell fractionation
Homogenation
Ultracentrifugation
Graticule
Eukaryote
Organelle
Nucleus
Mitochondria
Chloroplast
Rough endoplasmic reticulum (RER)
Smooth endoplasmic reticulum (SER)
Golgi
Lysosome
Ribosome
Cell Wall
Vacuole
Tissue
Organ
Organ system
Prokaryote
Bacteria
Plasmid
Flagellum
Virus
Capsid
Attachment Protein
Cell Division
Mitosis
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Daughter cell
Chromatid
Centromere
Interphase
Prophase
Metaphase
Anaphase
Telophase
Cytokinesis
Spindle fibres
Centrioles
Equator
Binary Fission
Cancer
Tumour
Transport across membrane
Plasma Membrane
Phospholipid
Bilayer
Protein Channel
Carrier Protein
Glycoprotein
Glycolipid
Cholesterol
Permeability
Fluid mosaic model
Diffusion
Facilitated diffusion
Osmosis
Water potential
Isotonic
Active transport
Co-transport
Immunity
Pathogen
Self
Foreign (non-self)
Antigen
Lymphocyte
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Phagocyte
Phagocytosis
Lysosome
Phagosome
Antigen-presentation
Cell-mediated immunity
T Cells
Clonal Selection
TH cells (helper T cell)
TC cells (cytotoxic T cells)
Humoral Immunity
B Cells
Plasma B Cells
Antibody
Memory B Cells
Monoclonal antibodies
Primary Response
Secondary response
Passive immunity
Active immunity
Natural immunity
Artificial immunity
Vaccination
Herd immunity
Antigenic variability
HIV
Reverse transcriptase
Retrovirus
mRNA
AIDS
ELISA test
Antibiotic
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Q1. Metabolism is the sum of all the chemical reactions in the cells of the body.
One metabolic reaction is the formation of lipids.
(a) Give one other metabolic reaction in cells.
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(1)
Table 1 shows the mean metabolic rate of humans of different ages.
Table 1
Age in
years
Mean metabolic rate in kJ/m2/hour
Males Females
5 53 53
15 45 42
25 39 35
35 37 35
45 36 35
(b) What two conclusions can be made from the data in Table 1?
Tick two boxes.
As age increases, mean metabolic rate of males and
females increases.
Males have a higher metabolic rate than females after
five years of age.
The mean metabolic rate of females decreases faster
than males up to 25 years of age.
The mean metabolic rate of males and females decreases
more quickly after the age of 35.
There is no relationship between age and mean
metabolic rate.
(2)
(c) Calculate the percentage decrease in the mean metabolic rate of males between 5 years and
45 years of age.
Use the equation:
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Give your answer to 3 significant figures.
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Percentage decrease = ___________________
(3)
Regular exercise can increase metabolic rate.
Two people did five minutes of gentle exercise from rest.
Table 2 shows the effect of the exercise on their heart rates.
Table 2
Time in
minutes
Heart rate in beats per
minute
Person R Person S
0 (at rest) 60 78
1 76 100
2 85 110
3 91 119
4 99 129
5 99 132
(d) Describe two differences in the response of person R and person S to the exercise.
Use information from Table 2.
1. _________________________________________________________________
___________________________________________________________________
2. _________________________________________________________________
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(2)
(e) Complete the line graph below for person S.
You should:
• add the scale to the x axis
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• label the x axis.
(4)
(f) After five minutes of exercise, the heart rate of person S was 132 beats per minute. When
person S rested, his heart rate decreased steadily at a rate of 12 beats every minute.
Calculate how much time it would take the heart rate of person S to return to its resting
rate.
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Time = ___________________ minutes
(2)
(g) A student made the following hypothesis about the heart rate of smokers and non-smokers
during exercise.
“During exercise, the heart rate of smokers increases more than
the heart rate of non-smokers.”
Design an investigation that would allow you to test this hypothesis.
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(6)
(Total 20 marks)
Q2. In South Asia, forests are being cleared to grow palm oil trees. The palm oil is mainly used to
produce fuel for motor vehicles.
The graph shows the production of palm oil in one South Asian country.
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(a) Calculate the mean increase in palm oil production per year for the five year period 2000 to
2005.
Show clearly how you work out your answer.
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Mean increase = _______________ millions of tonnes per year
(2)
(b) Clearing forests and replacing the forests with palm oil trees to produce fuel for motor
vehicles will affect the composition of the atmosphere.
Explain how.
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(5)
(Total 7 marks)
Q3. (a) The graph shows the effect of pH on the activities of three enzymes, X, Y and Z.
These enzymes help to digest food in the human digestive system.
Each enzyme is produced by a different part of the digestive system.
pH
(i) What is the optimum (best) pH for the action of enzyme Z?
__________________
(1)
(ii) The stomach makes a substance that gives the correct pH for enzyme action in the
human stomach.
Name this substance. ____________________________________________
(1)
(iii) Which enzyme, X, Y or Z, will work best in the human stomach?
__________________
(1)
(b) In this question you will be assessed on using good English, organising information clearly and using
specialist terms where appropriate.
Different parts of the human digestive system help to break down molecules of fat so that
they can be absorbed into the body.
Describe how.
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To gain full marks you should refer to:
• the enzyme and where the enzyme is produced
• the products of digestion
• any other chemicals involved.
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(6)
(Total 9 marks)
Q4. Biological detergents contain protease enzymes.
(a) The drawings show some apparatus and materials.
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In this question you will be assessed on using good English, organising information clearly and using
specialist terms where appropriate.
Describe how you would use the apparatus and materials shown in the drawings to find the
best temperature for removing stains from clothing.
You should include how you would make the investigation a fair test.
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(6)
(b) In a similar investigation a student investigated the effect of pH on the time taken to
remove a stain from pieces of cloth.
The table shows the student’s results.
pH of detergent solution
1 2 3 4 5 6 7 8 9
Time taken to
remove
stain in minutes
20 19 17 14 10 4 8 12 16
(i) On the graph paper below draw a graph to show the student’s results.
• Add a suitable scale and label to the y axis.
• Plot the student’s results.
• Draw a line of best fit.
pH of detergent solution
(4)
(ii) Which is the best pH for using the detergent?
pH _____________________________
(1)
(c) Scientists investigated the stability of a protease enzyme. The protease enzyme was
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extracted from plants.
The scientists:
• pre-incubated samples of the enzyme at various temperatures for 30 minutes
• put each sample on ice for a further 10 minutes
• measured the percentage (%) remaining activity of the enzyme in each sample.
This was done by incubating each sample with protein at 37 °C for 6 hours.
The graph shows the scientists’ results.
Pre-incubation temperature in °C
The scientists recommended that the enzyme could be used in detergents at a temperature
of 60 °C.
Suggest why the scientists recommended a temperature of 60 °C.
Use information from the graph and your own scientific knowledge in your answer.
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(3)
(Total 14 marks)
Q5. The graph shows the core body temperature and the skin surface temperature of a cyclist before,
during and after a race.
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Start
of race
(a) (i) When the cyclist finished the race, his core body temperature started to decrease.
How long did the race last?
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(1)
(ii) Describe and explain the different patterns shown in the core body temperature and
skin surface temperature between 09.15 and 10.15.
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(6)
(iii) After 10.30, the core body temperature decreased.
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Explain how changes in the blood vessels supplying the skin caused the skin surface
temperature to increase.
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(2)
(b) During the race, the cyclist’s blood glucose concentration began to decrease.
Describe how the body responds when the blood glucose concentration begins to
decrease.
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(3)
(Total 12 marks)
Q6. It is important to remove waste products from our bodies.
Healthy kidneys help to keep our internal environment constant.
(a) Describe how a healthy kidney produces urine.
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(5)
(b) A child has kidney failure and is treated with dialysis.
Before the dialysis starts, the doctor measures the concentration of urea and glucose in the
child’s blood.
The table shows the results.
Concentration in the
blood before dialysis
starts in mmol per dm3
Urea 28
Glucose 6
The child has a normal blood glucose concentration.
(i) Sketch a graph on Figure 1 to suggest what will happen to the concentration of urea
in the blood during dialysis.
(1)
(ii) Sketch a graph on Figure 2 to suggest what will happen to the concentration of
glucose in the blood during dialysis.
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(1)
(c) (i) Another way of treating kidney failure is with a kidney transplant.
A transplanted kidney can be rejected.
Explain why the new kidney may be rejected.
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(3)
(ii) Describe one way in which doctors try to prevent kidney rejection.
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(1)
(Total 11 marks)
Q7. A student plans an investigation using mould.
(a) Mould spores are hazardous.
Give one safety precaution the student should take when doing this investigation.
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(1)
(b) A student made the following hypothesis about the growth of mould:
‘The higher the temperature, the faster the growth of mould’.
The student planned to measure the amount of mould growing on bread.
The student used the following materials and equipment:
• slices of bread
• sealable plastic bags
• a knife
• a chopping board
• mould spores.
Describe how the materials and equipment could be used to test the hypothesis.
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(4)
(c) Give one variable the student should control in the investigation.
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(1)
(d) Another student did a similar investigation.
The diagram below shows the results.
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Determine the rate of mould growth at 42 °C between day 2 and day 7.
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Rate of mould growth = _______________ units per day
(2)
(e) The growth of mould shows decomposition of the bread.
Give a conclusion about decomposition from the results in the diagram above.
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(1)
(Total 9 marks)
Q8. An athlete ran as fast as he could until he was exhausted.
(a) Figure 1 shows the concentrations of glucose and of lactic acid in the athlete’s blood at the
start and at the end of the run.
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(i) Lactic acid is made during anaerobic respiration.
What does anaerobic mean?
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(1)
(ii) Give evidence from Figure 1 that the athlete respired anaerobically during the run.
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(1)
(b) Figure 2 shows the effect of running on the rate of blood flow through the athlete’s
muscles.
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(i) For how many minutes did the athlete run?
Time = ______________________ minutes
(1)
(ii) Describe what happens to the rate of blood flow through the athlete’s muscles during
the run.
Use data from Figure 2 in your answer.
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(2)
(iii) Explain how the change in blood flow to the athlete’s muscles helps him to run.
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(4)
(Total 9 marks)
Q9. Malaria is a disease caused by a microorganism carried by mosquitoes.
The microorganism is transferred to humans when adult female mosquitoes feed on human blood.
The figure below shows the life cycle of a mosquito.
© watcharapon/iStock
The World Health Organisation estimates that 3 × 108 people are infected with malaria every
year.
Scientists estimate that malaria kills 2 × 106 people every year.
The people who are infected with malaria but do not die, may be seriously ill and need health care
for the rest of their lives.
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(a) Based on the estimated figures, what percentage of people infected with malaria die from
the disease?
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(2)
(b) An internet article states:
1 Mosquito larvae are at the start of the food chain for some fish.
2 Adult mosquitoes provide food for bats and birds.
3 Mosquitoes are also important in plant reproduction because they feed from flowers
of crop plants.
(i) The first sentence in the article is not correct.
Explain why.
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(2)
(ii) A company plans to produce genetically modified (GM) adult male mosquitoes.
The GM mosquitoes will carry a gene from bacteria. The gene causes the death of
offspring before they become adults.
Male mosquitoes do not feed on blood.
Scientists are considering releasing millions of adult male GM mosquitoes into the
wild.
Do you think scientists should release millions of male GM mosquitoes into the wild?
In your answer you should give advantages and disadvantages of releasing GM
mosquitoes into the wild.
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(4)
(iii) Describe the process for creating a GM mosquito.
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(3)
(Total 11 marks)
Q10. Some students investigated the size of a population of dandelion plants in a field.
The diagram below shows the field.
The students:
• placed a 1 m × 1 m square quadrat at 10 random positions in the field
• counted the number of dandelion plants in each quadrat.
The table below shows the students’ results.
Quadrat
number
Number of
dandelion plants
1 6
2 9
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3 5
4 8
5 0
6 10
7 2
8 1
9 8
10 11
(a) Why did the students place the quadrats at random positions?
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(1)
(b) Estimate the total number of dandelion plants in the field.
Calculate your answer using information from the diagram and the table above.
Give your answer in standard form.
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Total number of dandelion plants = ____________________
(5)
Quadrats 5, 7 and 8 were each placed less than 10 metres from the woodland.
These quadrats contained low numbers of dandelion plants.
The students made the hypothesis:
‘Light intensity affects the number of dandelion plants that grow in an area.’
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(c) Plan an investigation to test this hypothesis.
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(6)
(d) Light is an environmental factor that affects the growth of dandelion plants.
Give two other environmental factors that affect the growth of dandelion plants.
1. _________________________________________________________________
2. _________________________________________________________________
(2)
(Total 14 marks)
Q11. Table 1 shows information about some food components in cow’s milk.
Table 1
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Value per
500 cm3
Recommended Daily
Allowance (RDA) for
a typical adult
Energy in kJ 1046 8700
Fat in g 8.4 70.0
Salt in g 0.5 6.0
Calcium in mg 605 1000
Vitamin B-12 in µg 4.5 2.4
(a) How much more milk would a typical adult have to drink to get their RDA for calcium
compared with the amount of milk needed to get their RDA for vitamin B-12?
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Volume of milk = ______________________ cm3
(3)
(b) Describe how a student could test cow’s milk to show whether it contains protein and
different types of carbohydrate.
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(6)
A scientist investigated the effect of bile on the breakdown of fat in a sample of milk.
The scientist used an indicator that is colourless in solutions with a pH lower than 10, and pink in
solutions with a pH above 10.
This is the method used.
1. Add 1 drop of bile to a test tube and one drop of water to a second test tube.
2. Add the following to each test tube:
• 5 cm3 of milk
• 7 cm3 of sodium carbonate solution (to make the solution above pH 10)
• 5 drops of the indicator
• 1 cm3 of lipase.
3. Time how long it takes for the indicator in the solutions to become colourless.
The results are shown in Table 2.
Table 2
Time taken for the indicator to
become colourless in seconds
Solution with bile 65
Solution without bile 143
(c) Explain why the indicator in both tubes became colourless.
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(3)
(d) Give the reason why the measurement of the time taken for the indicator to become
colourless might be inaccurate.
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(1)
(e) Explain the difference in the results for the two test tubes in Table 2.
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(3)
(Total 16 marks)
Q12. Catalase is an enzyme found in many different tissues in plants and animals. It speeds up the rate
of the following reaction.
hydrogen peroxide water + oxygen
Figure 1 shows a 25-day-old broad bean seedling.
Some students investigated whether different parts of bean seedlings contained different amounts
of catalase.
The students:
• put hydrogen peroxide into five test tubes
• added a different part of a bean seedling to each tube
• recorded the results after half a minute.
If there was catalase in part of the seedling, oxygen gas was given off.
When oxygen gas is given off, foam is produced in the tubes.
Figure 2 shows the results.
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The students made the following conclusions:
• most parts of a bean seedling contain catalase
• the seed contains a lot of catalase
• stems and roots have quite a lot of catalase
• the leaves have a little bit of catalase
• the seed coat has hardly any catalase.
The students’ teacher said that the students needed to improve their investigation in order to
make valid conclusions.
(a) In this question you will be assessed on using good English, organising
information clearly and using specialist terms where appropriate.
Describe how you would carry out an investigation to compare the amounts of catalase in
different parts of bean seedlings.
You should include details of how you would make sure your results give a valid
comparison of the amounts of catalase.
___________________________________________________________________
___________________________________________________________________
___________________________________________________________________
___________________________________________________________________
___________________________________________________________________
___________________________________________________________________
___________________________________________________________________
___________________________________________________________________
___________________________________________________________________
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___________________________________________________________________
(6)
(b) Scientists investigated the effect of pH on the activity of the enzyme catalase in a fungus.
The table below shows the scientists’ results.
pH
Enzyme activity in arbitrary units
Test 1 Test 2 Test 3 Test 4 Test 5 Mean
3.0 0 0 0 0 0 0
4.0 6 5 8 4 7 6
5.0 38 65 41 42 39
5.5 80 86 82 84 88 84
6.0 100 99 96 103 102 100
6.5 94 92 90 93 91 92
7.0 61 63 61 62 63 62
8.0 22 22 21 24 21 22
(i) Calculate the mean enzyme activity at pH 5.0.
______________________________________________________________
______________________________________________________________
Mean = _____________ arbitrary units
(2)
(ii) On the graph paper in Figure 3, draw a graph to show the scientists’ results.
Remember to:
• add a label to the vertical axis
• plot the mean values of enzyme activity
• draw a line of best fit.
Figure 3
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(4)
(iii) At what pH does the enzyme work best?
____________________
(1)
(iv) Predict the activity of the enzyme at pH 9.0.
____________________ arbitrary units
(1)
(v) Suggest why the enzyme’s activity at pH 3.0 is zero.
______________________________________________________________
______________________________________________________________
(1)
(Total 15 marks)
Q13. During exercise, the heart beats faster and with greater force.
The ‘heart rate’ is the number of times the heart beats each minute.The volume of blood that
travels out of the heart each time the heart beats is called the ‘stroke volume’.
In an investigation, Person 1 and Person 2 ran as fast as they could for 1 minute. Scientists
measured the heart rates and stroke volumes of Person 1 and Person 2 at rest, during the
exercise and after the exercise.
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The graph below shows the scientists’ results.
(a) The ‘cardiac output’ is the volume of blood sent from the heart to the muscles each minute.
Cardiac output = Heart rate × Stroke volume
At the end of the exercise, Person 1’s cardiac output = 160 × 77 = 12 320 cm3 per
minute.
Use information from the figure above to complete the following calculation of Person 2’s
cardiac output at the end of the exercise.
At the end of the exercise:
Person 2’s heart rate = _______________ beats per minute
Person 2’s stroke volume = _______________ cm3
Person 2’s cardiac output = _______________ cm3 per minute
(3)
(b) Person 2 had a much lower cardiac output than Person 1.
(i) Use information from the figure above to suggest the main reason for the lower
cardiac output of Person 2.
______________________________________________________________
______________________________________________________________
(1)
(ii) Person 1 was able to run much faster than Person 2.
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Use information from the figure above and your own knowledge to explain why.
______________________________________________________________
______________________________________________________________
______________________________________________________________
______________________________________________________________
______________________________________________________________
______________________________________________________________
______________________________________________________________
______________________________________________________________
______________________________________________________________
______________________________________________________________
(5)
(Total 9 marks)
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Summer Work Mark Schemes
Q1.
(a) any one from:
• respiration
• formation of proteins
• formation / breakdown of glycogen
• breakdown of (excess) protein or formation of urea
• photosynthesis or formation of glucose / starch (in plants)
ignore formation of carbohydrates
1
allow other correct reference to metabolic reactions in
cells
ignore reference to digestion
(b) males have a higher metabolic rate than females after five years of age
1
the mean metabolic rate of females decreases faster than males up to 25 years of
age
1
each additional tick negates a mark
(c)
1
32.075472…
allow correct rounding of this to at least 4 significant
figures
1
32.1
allow a correct reduction to 3 significant figures from an
incorrect calculation for marking point 2
1
an answer of 32.1 scores 3 marks
(d) any two from:
allow converse
• (person) R heart rate rose / increased more slowly than (person) S
• (person) R heart rate levelled off whereas (person) S continued to increase
• (person) R heart rate rose less (overall / after 5 minutes of exercise) than S
allow correct use of figures
e.g. R increased (overall) by 39 bpm / 65% and S by 54
bpm / 69%
ignore lack of units
2
(e) correct scale and axis labelled
allow min(s)
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do not accept ‘m’
the zero is not required on the x-axis
1
all points plotted correctly (to within ± ½ square)
allow 4 or 5 correct plots for 1 mark
2
line joined point to point or correct curved line of best fit
1
(f)
allow sequential deductions of 12 four or five times
1
4.5 (minutes) / 4½ minutes / 4 minutes 30 seconds / 4:30
do not accept 4:50 or 4 minutes 50 seconds
1
an answer of 4.5 minutes scores 2 marks
(g) Level 3: The method would lead to the production of a valid outcome. All key
steps are identified and logically sequenced.
5−6
Level 2: The method would not necessarily lead to a valid outcome. Most steps
are identified, but the method is not fully logically sequenced.
3−4
Level 1: The method would not lead to a valid outcome. Some relevant steps are
identified, but links are not made clear.
1−2
No relevant content
0
Indicative content
• two groups of people − non-smokers and smokers
• have at least five people in each group or large groups
• get each person to do (named) exercise
• controlled variables:
− same number of people in each group or large groups
− same gender
− same level of activity / exercise
− same age
− no health issues / illnesses
− same type of exercise
− same time for exercise
• record heart rate for each person before and after exercise
• calculate increase in heart rate for each person after exercise
• compare results for each group
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for level 3, students should refer to at least 5 smokers and 5 non-smokers,
carrying out exercise with control variables and a means of determining an increase
in heart rate
for level 2, students should refer to ‘groups’ of smokers and non-smokers
exercising
[20]
Q2.
(a) 860
correct answer gains 2 marks
if answer incorrect evidence of (6100 - 1800) ÷ 5
or 4300 ÷ 5
or (900 + 600 + 1000 + 700 + 1100) ÷ 5 gains 1 mark
allow ecf from 1 incorrect graph reading
2
(b) ignore references to oxygen / sulfur dioxide / nitrogen oxides / acid rain
ignore global warming
Effects of deforestation
deforestation increases the amount of carbon dioxide in the atmosphere
award this point only if linked to deforestation
1
any two from:
• due to less photosynthesis or less carbon dioxide taken in
or carbon dioxide not locked up in (forest) trees
• due to burning of forest / from machinery
• due to activity of microorganisms / decay
2
Effects of growing palm for fuel
carbon dioxide released when palm oil used as fuel
1
(eventually) CO2 intake and output might balance out or burning palm
oil carbon neutral
accept less carbon dioxide than from burning fossil fuels
1
[7]
Q3.
(a) (i) 8.6
accept value in range 8.5 to 8.7
1
(ii) hydrochloric acid / HCl
accept HCL
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accept hydrogen chloride
ignore hcl / etc.
1
(iii) X
1
(b) Marks awarded for this answer will be determined by the Quality of Written
Communication (QWC) as well as the standard of the scientific response.
Examiners should also refer to the information in the Marking guidance.
0 marks
No relevant content.
Level 1 (1-2 marks)
There is a simple description of part of a process or a reference to at least one of:
mechanical digestion, lipase, product of enzyme action, bile, site of production or
site of digestion
Level 2 (3-4 marks)
There is a description of at least one process linking ideas
Level 3 (5-6 marks)
There is a clear description of the process including reference to the majority of:
mechanical digestion, lipase, bile, where they are produced, products, function of
bile and site of digestion / absorption
Examples of biological points made in the response:
• mechanical breakdown in mouth / stomach
• fats →fatty acids and / or glycerol
• by lipase
• (produced by) pancreas
• and small intestine
• fat digestion occurs in small intestine
• bile
• produced by liver
• neutralises acid from stomach
• produces alkaline conditions in intestine
• refs. to increased surface area related to emulsification or chewing
• products are small molecules / water-soluble
• products absorbed by small intestine
6
[9]
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45
Q4.
(a) Marks awarded for this answer will be determined by the Quality of Communication
(QC) as well as the standard of the scientific response. Examiners should also refer to the
information on page 5, and apply a ‘best − fit’ approach to the marking.
0 marks
No relevant content.
Level 1 (1 − 2 marks)
The method described is weak and could not be used to collect valid results
however does show some understanding of the sequence of an investigation.
Level 2 (3 − 4 marks)
The method described could be followed and would enable some results to be
collected but lacks detail.
Level 3 (5 − 6 marks)
The method described could be easily followed and would enable valid results to
be collected.
examples of biology points made in the response:
• (use of measuring cylinder to) measure equal volumes of detergent solution
• (use of dropping bottle to) apply same number of drops / amount of stain to each
piece of cloth
• include stainless cloth as control
• use of forceps to transfer cloths
• use of test tubes as containers for detergent solution + stained cloth
• use water bath to provide a range of temperatures
• cloths left in detergent solution at each temperature
• for same length of time or measure time taken to remove stain
• repetition
• assessing the stain removal
6
(b) (i) y axis: labelled ‘Time (taken to remove stain in) minutes’ plus suitable scale
data spread greater than half of grid
1
points or bars plotted correctly to within ± 1 mm
deduct 1 mark for each incorrect plot up to a maximum of 2
2
one suitable line of best fit drawn on graph
not feathery
not extrapolated to (0,0)
not point to point as on this occasion it is inappropriate
1
(ii) 6 ± 0.1
accept ecf from student graph
1
(c) activity of enzyme still very high / 84% / over 80%
or
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46
only lost 15% / 16% activity
allow above 60 °C marked decrease in activity
allow 85%
1
any two from:
• rate of reaction high at 60 °C / higher than at lower temperatures
allow in terms of reaction kinetics / collisions
• higher temperatures would increase (energy) costs
or
might damage cloth
ignore enzyme denaturation
• higher temperatures / 60 °C is better (than lower temperatures) to remove other
stains / named stains
or
better for killing bacteria / infection control
eg grease
2
[14]
Q5.
(a) (i) 1 hour 15 mins / 1.25 hours / 75 mins
allow 1:15
ignore 1.15 hours
1
(ii) increase in (core / body) temperature
ignore numbers
1
(due to an) increase in respiration or more muscle contraction
1
releasing energy (as a waste product)
allow produces ‘heat’
do not allow making energy
1
skin temperature decreases
1
(because there is) sweating
1
(which) evaporates and cools the skin
ignore references to vasodilation or vasoconstriction
1
(iii) (there is) dilation of vessels (supplying skin capillaries)
allow vasodilation
allow blood vessels widen
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ignore expand
do not accept dilating capillaries or moving vessels
1
(so) more blood flows (near skin) (surface) or blood is closer (to the skin)
ignore ref to heat
1
(c) pancreas detects (low) blood glucose
1
produces glucagon
do not allow glucagon made in the liver
1
(so) glycogen is converted to glucose
allow adrenaline released which increases conversion of glycogen to
glucose
or
reduced insulin production so less glucose into cells / less glucose
converted to glycogen
for 1 mark
1
[12]
Q6.
(a) (the kidney) filters the blood
ignore refs to hormones and drugs
1
(and then) reabsorbs all of the glucose
1
reabsorbs some of the ions
allow salts
ignore minerals
1
reabsorbs some of the water
1
releases urea (in urine)
1
(b) (i) should fall from 28 (to the end of dialysis)
ignore any line drawn after end of dialysis
allow + / - 0.5 square
graph line must fall to / below
below 15
1
(ii) should stay level at about 6 throughout
ignore slight variations
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allow + / - 1 square
ignore any line drawn after end of dialysis
1
(c) (i) immune system
allow white blood cells / lymphocytes
1
(produces) antibodies
1
(which) attack the antigens (on the transplanted kidney)
non-matching antigens insufficient
1
(ii) any one from:
• tissue typing (to find match)
• treating with drugs that suppress the immune system
accept treat with immunosuppressants.
1
[11]
Q7.
(a) wear a face mask
allow wear gloves
1
(b) Level 2 (3–4 marks):
A detailed and coherent plan covering all the major steps. It sets out the steps needed in
a logical manner that could be followed by another person to produce an outcome which
will address the hypothesis.
Level 1 (1–2 marks):
Simple statements relating to steps are made but they may not be in a logical order. The
plan may not allow another person to produce an outcome which will address the
hypothesis.
0 marks:
No relevant content.
Indicative content
Plan:
• cut a specified number of pieces of bread to the same size
• place mould spores on the bread
• the number of mould spores needs to be the same quantity of mould spores on
each piece of bread
• place bread in different sealable plastic bags
• place in different temperatures (minimum of three) eg fridge, room, incubator
• leave each for the same amount of time eg four days
• measure the percentage cover of mould on each piece of bread
• repeat experiment
additional examiner guidance:
• good level 2 answer will describe how the growth of mould can be measured and
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49
will give a range of different temperatures to be used
• allow equivalent levels of credit for alternative methodologies that would clearly
produce a measurable outcome in terms of mould growth at various temperatures
4
(c) any one from:
• type of mould
• amount of mould (put on each piece of bread)
• amount of air in the plastic bags
• size of the pieces of bread
• type of bread
• amount of moisture / water added
1
(d) (56 − 4 = 52) / 5
1
10.4
allow 10.4 with no working shown for 2 marks
1
ecf for incorrectly read figures for 1 mark
(e) (decomposition occurs at a faster rate when the temperature is higher
or
amount of decomposition is higher when temperature is higher
1
[9]
Q8.
(a) (i) without oxygen
allow not enough oxygen
ignore air
ignore production of CO2
ignore energy
1
(ii) more / high / increased lactic acid (at end)
allow approximate figures (to show increase)
ignore reference to glucose
1
(b) (i) 1.5
allow only 1.5 / 1½ / one and a half
1
(ii) increases at first and levels off
ignore subsequent decrease
1
suitable use of numbers eg
rises to 10 / by 9 (dm3 per min)
or
increases up to 1.5 (min) / levels off after 1.5 (min) (of x axis timescale)
allow answer in range 1.4 to 1.5
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or
after the first minute (of the run)
1
(iii) supplies (more) oxygen
1
supplies (more) glucose
1
need ‘more/faster’ once only for full marks
allow removes (more) CO2 / lactic acid / heat as an alternative for
either marking point one or two, once only
for (more) respiration
1
releases (more) energy (for muscle contraction)
do not allow energy production or for respiration
1
[9]
Q9.
(a) 0.67(%)
allow
allow 1 mark for evidence of (2 × 106) ÷ (3 × 108)
or
allow 1 mark for 0.0067 or 0.6
2
(b) (i) idea that food chains start with plants / producers
allow food chains do not start with animals or larvae are consumers
1
idea that these make food (for other organisms in the chain)
allow idea that plants / producers photosynthesise or plants /
producers get energy from the sun
allow mosquito larvae do not make food / photosynthesise or
mosquito larvae do not get energy from the sun
1
(ii) any four from:
• reasoned argument for or against release
must refer to at least one advantage and one disadvantage.
max 3 marks for either only advantages or only disadvantages
advantages:
• fewer mosquitos biting or spreading malaria
• fewer people get / die from malaria
allow people won’t get / die from malaria
• lower medical costs (for those infected or for treatment) or less healthcare
needed
• better economically for developing / tropical countries.
disadvantages:
• fewer crops reproduce
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allow fewer crops pollinated
• poorer crop yield
• possible starvation (of people)
• high cost of GM production / mosquito release
• less food for bats / birds or bats / birds die
allow disruption to food chain / ecosystem or reduction of biodiversity
• gene could ‘escape’ into other wildlife / species
ignore into plants
4
(iii) any three from:
• gene from bacteria cut out
allow allele for gene
• ref to enzymes (anywhere in process)
allow at any point in process, ie in cutting or in splicing
• (gene) transferred to chromosome of mosquito
allow DNA for chromosome
• at an early stage of development
allow egg / embryo
3
[11]
Q10.
(a) there is an uneven distribution of dandelions
or
(more) representative / valid
or
avoid bias
or
more accurate / precise mean
ignore accurate / precise unqualified
ignore repeatability / reproducibility / reliability / fair test
1
(b) (correct mean per m2 =) 6 or 6.0
1
(correct field area =) 55 000 (m2)
1
mean × area − e.g. 6(.0) × 55 000
allow incorrect calculated values for mean and / or field
area
1
330 000
allow correct calculation from previous calculation
1
3.3 × 105
allow calculated value in standard form
1
an answer of 3.3 × 105 scores 5 marks
an answer of 330 000 scores 4 marks
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52
(c) Level 3: The method would lead to the production of a valid outcome. All key
steps are identified and logically sequenced.
5−6
Level 2: The method would not necessarily lead to a valid outcome. Most steps
are identified, but the method is not fully logically sequenced.
3−4
Level 1: The method would not lead to a valid outcome. Some relevant steps are
identified, but links are not made clear.
1−2
No relevant content
0
Indicative content
• placing of quadrat
• large number of quadrats used
• how randomness achieved − e.g. table of random numbers or random
number button on calculator or along transect
• quadrats placed at coordinates or regular intervals along transect
• in each of two areas of different light intensities or transect running through
areas of different light intensity
• for each quadrat count number of dandelions
• for each quadrat measure light intensity
• compare data from different light intensity
to access level 3 the key ideas of using a large number of quadrats randomly, or
along a transect, and counting the number of dandelions in areas of differing light
intensity need to be given to produce a valid outcome
(d) any two from:
• temperature
allow heat
• water
allow moisture / rain
• (soil) pH
allow acidity
• minerals / ions
allow e.g. magnesium ions or nitrate
allow salts / nutrients
• winds
• herbivores
allow trampling
ignore carbon dioxide
ignore space
ignore competition unqualified
do not accept oxygen
2
[14]
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Q11.
(a) (for calcium)
allow any correct rounding to minimum 3 significant
figures
allow alternative route with correct rounding
1
(for vitamin B-12)
allow alternative route with correct rounding
1
560 / 559.8 / 559.78 / 559 (cm3)
allow only correct answer based on values given for
vitamin B-12 and calcium
1
an answer of 560 / 559.8 / 559.78 / 559 (cm3) scores
3 marks
an incorrect answer for one step does not prevent
allocation of marks for subsequent steps
(b) Level 2: Scientifically relevant facts, events or processes are identified and given in
detail to form an accurate account.
4−6
Level 1: Facts, events or processes are identified and simply stated but their
relevance is not clear.
1−3
No relevant content
0
Indicative content
• Biuret reagent (allow CuSO4 and NaOH) tests for protein
• add Biuret reagent to milk
• solution will turn (from blue) to lilac if positive
• iodine solution tests for starch (ignore iodine unqualified)
• add iodine solution to milk
• solution will turn (from orange / brown) to blue / black if positive
• Benedict’s reagent tests for sugars
• add Benedict’s reagent to milk and boil / heat (allow any temperature above 60 °C)
• solution will turn (from blue) to (brick) red / brown / orange / yellow / green if
positive
for level 2, reference to all three food tests is required
(c) lipase breaks down fat into fatty acids (and glycerol)
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do not accept if ‘glycerol’ is contradicted
1
(and) fatty acids lower the pH
1
(and when) fatty acids cause the pH to be below 10 (the indicator becomes
colourless)
1
(d) observation of colour change is subjective / based on opinion
ignore human error unqualified
ignore experimental error or examples of this
1
(e) bile emulsifies fats
allow a correct description of emulsification (i.e. breaks
fat from large droplets into smaller droplets)
do not accept a description of chemical breakdown
1
creates a larger surface area (of fat)
1
(so) lipase can break down fat (to produce fatty acids) more quickly / effectively
allow fatty acids produced by action of lipase more
quickly
1
[16]
Q12.
(a) Marks awarded for this answer will be determined by the Quality of Communication
(QC) as well as the standard of the scientific response. Examiners should also refer to the
information in the Marking guidance and apply a ‘best-fit’ approach to the marking.
0 marks
No relevant content.
Level 1 (1−2 marks)
The method described is weak and could not be used to collect valid results,
however does show some understanding of the sequence of an investigation.
Level 2 (3−4 marks)
The method described could be followed and would enable some valid results to
be collected, but lacks detail.
Level 3 (5−6 marks)
The method described could be easily followed and would enable valid results to
be collected.
Examples of the points made in the response:
• bean seedlings of same age
• cut material from same part of each organ (for repeats) e.g. top 1 cm of
stem / a whole cotyledon / seed
• equal mass of each organ
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accept weight for mass
• grind / homogenise
• in equal amounts of water / buffer
• equal volumes of hydrogen peroxide solution
• equal concentrations of hydrogen peroxide solution
• same temperature
• temperature maintained in water bath
• quantitative measure of gas production eg height of foam in mm / collect gas
in graduated syringe in cm3
• for same time period
• repetitions (3+ times)
• calculate mean for each.
6
(b) (i) correct answer: 40
1 mark for 45 as the anomalous result has been included in the
calculation
or
1 mark for
or
2
(ii) vertical axis correctly labelled:
‘Enzyme activity in arbitrary units’
allow ecf from (b)(i)
1
points plotted correctly ±1 mm
deduct 1 mark for each incorrect plot
2
suitable line of best fit
not feathery, not point to point
1
(iii) 6.0 / 6
allow ± 0.1
if 6.0 not given, allow correct for candidate’s graph ± 0.1
1
(iv) in range 0 to 14 units
allow correct for candidate’s graph
1
(v) enzyme denatured / enzyme (active site) shape changed
allow substrate no longer fits (active site)
ignore reference to temperature
do not allow enzyme dies
1
[15]
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Q13.
(a) 5624
allow 2 marks for:
• correct HR = 148 and correct SV = 38 plus wrong answer /
no answer
or
• only one value correct and ecf for answer
allow 1 mark for:
• incorrect values and ecf for answer
or
• only one value correct
3
(b) (i) Person 2 has low(er) stroke volume / SV / described
eg Person 2 pumps out smaller volume each beat
do not allow Person 2 has lower heart rate
1
(ii) Person 1 sends more blood (to muscles / body / lungs)
1
(which) supplies (more) oxygen
1
(and) supplies (more) glucose
1
(faster rate of) respiration or transfers (more) energy for use
ignore aerobic / anaerobic
allow (more) energy release
allow aerobic respiration transfers / releases more energy (than
anaerobic)
do not allow makes (more) energy
1
removes (more) CO2 / lactic acid / heat
allow less oxygen debt
or less lactic acid made
or (more) muscle contraction / less muscle fatigue
if no other mark awarded,
allow person 1 is fitter (than person 2) for max 1 mark
1