A immune-response A immune-response molecule which limits molecule which limits trypanosome infection of trypanosome infection of tsetse midguts. tsetse midguts. Mike Lehane Mike Lehane Liverpool School of Tropical Medicine Liverpool School of Tropical Medicine
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A immune-response molecule which limits trypanosome infection of tsetse midguts. Mike Lehane Liverpool School of Tropical Medicine.
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A immune-response molecule A immune-response molecule which limits trypanosome which limits trypanosome
infection of tsetse midguts.infection of tsetse midguts.
Mike LehaneMike Lehane
Liverpool School of Tropical MedicineLiverpool School of Tropical Medicine
MetacyclogenesMetacyclogenesisis
Midgut Midgut establishmenestablishmentt
1.
Complex differentiation to Complex differentiation to asymmetric dividing asymmetric dividing epimastigoteepimastigote + migration+ migration
2.
3.
T. bruceiT. brucei development in the development in the tsetse flytsetse fly
Trypanosome numbers (Log10) in the fly
0
1
2
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7
1 3 5 7 9 11 13 15
Days
Log
Successful infection
Midgut Cardia Salivary Gland
Trypanosome numbers (Log10) in the fly
0
1
2
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4
5
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7
1 3 5 7 9 11 13 15
Days
Log
Infection fails in the midgut
Log trypanosome numbers in the fly
0
1
2
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1 3 5 7 9 11 13 15
Days
Log
Prevalence of Trypanosome Infection vs Infective Bloodmeal
Trypanosome prevalence following knockdown (RNAi) of tsetseEP
Where is TsetseEP produced?
Is TsetseEP immune Is TsetseEP immune responsive?responsive?
2D gels of midgut protein:
A. 3 days post injection with PBS
B. 3 days post injection with live E. coli K12RM148
savannah riverine forest
TsetseEP is present in all tsetse species investigated and is
unique to Glossina.
Infected at indicated blood meal
Number of biological replicates Mean percentage infected following dsTsetseEPinjection (number of flies)
Mean percentage infectedfollowing dsAmp controlinjection (number of flies)
1 1 30.00 (30) 6.67 (15)
G. palpalis
SummarySummary The tsetse fly immune response The tsetse fly immune response
molecule TsetseEP affects the ability molecule TsetseEP affects the ability of trypanosomes to become of trypanosomes to become established in the midgut of tsetse established in the midgut of tsetse flies.flies.