397 | Page A Comparative study of Antimicrobial activity of Datura (Leaf, Seed), Neem (Leaf, Bark) and Orange (Peel, waste) on Escherichia coli and Paenibacillus barcinonesis Vivek Mishra 1 , Deepesh Kumar 2 , V.K.Srivastava 3 , Ashish shukla 4 , Aarti Gautam 5 , Shivani Shukla 6 , Richa Saxena 7 , Abhishek Upadhyay 8 , Saksham khusharia 9 1,2,3,4,5,7,8,9 Post Graduate Department of Biotechnology, RBS Engineering Technical Campus, Bichpuri, Agra, AKTU Lucknow, (India) 6 Department of Bioengineering, Integral University, Lucknow, India ABSTRACT In this present study was conducted to identify bacteria (Paenibacillus barcinonesis) by using biochemical test and then to identify antimicrobial investigation against (leaf extract, bark extract) of Neem, (peel extract, waste extract) of Orange and (leaf extract, seed extract) of Datura which is collected from the local market and Raja Balwant singh Engineering Technical Campus Bichpuri, Agra (U.P.) India. Antimicrobial activity of crude extract (Methanol, Isopropanol, Chloroform, Water, Butanol) were tested by using Well Diffusion Method against two Bacterial strains (Escherichia coli and Paenibacillus barcinonesis). Neem leaves methanol crude extract produced maximum zone of inhibition at 24 hrs, 48hrs and (2.26 ± 0.05 cm), (2.5 ± 0.17cm) and (2.6 ± 0.17cm) respectively against Escherichia coli. Neem leaves water crude extract produced maximum zone of inhibition at 24hrs, 48hrs and 72hrs (1.36 ± 1.01cm), (1.4 ± 0.1cm) and (1.5 ± 0.1cm) respectively against Paenibacillus barcinonesis. Methanol crude extract Bark of Neem showed maximum zone of inhibition at 24hrs, 48hrs and 72hrs (1.5 ± 0.43cm), (2.06 ± 0.05cm) and (2.13 ± 0.11cm) respectively against Escherichia coli. Methanol crude extract Bark of Neem showed maximum zone of inhibition at 24hrs, 48hrs and 72hrs (1.3 ± 0cm), (1.4 ± 0.05cm) and (1.36 ± 0.05cm) respectively against Paenibacillus barcinonesis. Orange peel methanol crude extract and butanol crude extract produced maximum zone of inhibition at 24hrs and 48hrs, 72hrs (1.4 ± 0.05cm) and (1.33 ± 0.11cm), (1.46 ± 0.15cm) respectively against Escherichia coli. Orange peel methanol crude extract and water crude extract produced maximum zone of inhibition at 24hrs and 48hrs, 72hrs (0.86 ± 0.05cm) and (0.83 ± 0.15cm), (0.86 ± 0.05cm) against Paenibacillus barcinonesis. Methanol and Butanol crude extract Waste of Orange showed ma++++++ximum zone of inhibition at 24hrs and 48hrs, 72hrs (1.6 ± 0.51cm) and (2.7 ± 0cm), (1.03 ± 0.05cm) against Escherichia coli. Chloroform crude extract Waste of Orange showed maximum zone of inhibition at 24hrs, 48hrs and 72hrs (0.9 ± 0.1cm), (0.9 ± 0.1cm) and (0.93 ± 0.05cm) against Paenibacillus barcinonesis. Methanol and Water crude extract leaves of Datura showed maximum zone of inhibition at 24hrs, 72hrs and 48hrs (1.36 ±
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397 | P a g e
A Comparative study of Antimicrobial activity of Datura
(Leaf, Seed), Neem (Leaf, Bark) and Orange (Peel, waste) on
Escherichia coli and Paenibacillus barcinonesis
Vivek Mishra1, Deepesh Kumar
2, V.K.Srivastava
3, Ashish shukla
4,
Aarti Gautam5, Shivani Shukla
6 , Richa Saxena
7, Abhishek Upadhyay
8,
Saksham khusharia9
1,2,3,4,5,7,8,9 Post Graduate Department of Biotechnology,