9th Annual Ronald C. Baird Sea Grant Science Symposium Microbiology of shell disease – which bacteria are responsible? Dr. Gordon Taylor –Stony Brook University Dr. Andrei Chistoserdov – Univ. Louisiana Dr. Patrick Gillevet – George Mason Univ. Dr. Michael Tlusty – New England
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9th Annual Ronald C. Baird Sea Grant Science Symposium Microbiology of shell disease – which bacteria are responsible? Dr. Gordon Taylor –Stony Brook University.
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9th Annual Ronald C. Baird Sea Grant Science Symposium
Microbiology of shell disease – which bacteria are responsible?
Dr. Gordon Taylor –Stony Brook University Dr. Andrei Chistoserdov – Univ. LouisianaDr. Patrick Gillevet – George Mason Univ.
Dr. Michael Tlusty – New England Aquarium
What type of bacteria settle onto lobster shell?
What type of bacteria firstattack the lobster shell?
What type of bacteria are presentas the lesion worsens?
Why do only some lobsters get
shell disease?
Compare bacteria in HealthyVs lesioned shellUse established community to
understand “pioneers”
What drives this Initial infection
Stony Brook Univ. – G. Taylor, B. Allam, A. McElroy, co-PIs;
S. Bell, Sea Grant Scholar, and T. Barrett, undergrad
George Mason UniversityC Ajuzie, M Sikaroodi, N Meres,
P Gillevet
Virginia Institute of Marine Sciences Jeff Shields et al
Compare bacteria of healthyto lesioned shell
Three similar but different methods
Lots of bacteria on the shell
Areas within a lobster differ….
Healthy vs diseased: No distinct differences
Can identify bacteria
A different analysis – same result
Changes in abundance
Peak 217bp (AluI digest) in lesion samples represented 4% of the total community profile and much less than 1% in healthy shell samples.
lesion samplegene fragment of pathogen or opportunistic species??
healthy shell sample
same fragment
Bacterial community activity(destructive enzyme rates)
What type of bacteria firstattack the lobster shell?
What type of bacteria are presentas the lesion worsens?
Why do only some lobsters get
shell disease?
Use established community to understand “pioneers”
Aquamarina ‘homaria’
Aquamarina ‘homaria’
•Based on 16S rRNA and phospholipid fatty acid composition is a species different from but closely related to A. muelleri.
•Not commonly found in the environment.
•Aquimarina muelleri is found in sediments, associated with algae and marine invertebrates. Apart from Arthropods, was detected only in a sea hatchery in Canada.
“A homaria” in other arthropodsSpecies Lesions Healthy
Carapace
Lobster 17 20
Spider Crab 10 8
Green Crab 7 3
Jonah Crab 9 8
Horseshoe Crab
4 3
Environmental Sampling• Samples taken from three different trips
1. Buzzards Bay Massachusetts, 11 locations2. Around Block Island, Rhode Island, 3. West Connecticut line to east Narragansett bay, Rhode Island