9.1 Manipulating DNA •Set up Cornell Notes on pg. 17 •Topic: 9.3 DNA Fingerprinting •Essential Question: 1.Describe two ways in which DNA fingerprinting is used. 2.1 Atoms, Ions, and Molecules 9.3 DNA Fingerprinting 1. Describe two ways in which DNA fingerprinting is used. KEY CONCEPT DNA fingerprints identify people at the molecular level Get out your homework: Who Ate the Cheese?
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9.1 Manipulating DNA Set up Cornell Notes on pg. 17 Topic: 9.3 DNA Fingerprinting Essential Question: 1.Describe two ways in which DNA fingerprinting is.
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9.1 Manipulating DNA•Set up Cornell Notes on pg. 17
•Topic: 9.3 DNA Fingerprinting
•Essential Question:
1.Describe two ways in which DNA fingerprinting is used.
2.1 Atoms, Ions, and Molecules
9.3 DNA Fingerprinting
1. Describe two ways in which DNA fingerprinting is used.
KEY CONCEPT DNA fingerprints identify people at the molecular level
Get out your homework: Who Ate the Cheese?
9.3 DNA Fingerprinting
KEY CONCEPT DNA fingerprints identify people at the molecular level.
9.3 DNA Fingerprinting
REVIEW from 9.1
• Gel electrophoresis is used to separate DNA fragments by size.– A DNA sample is cut with restriction enzymes.– Electrical current pulls DNA fragments through a gel.
9.3 DNA Fingerprinting
A DNA fingerprint is a type of restriction map.
DNA fingerprints are based on parts of an individual’s DNA that can be used for identification.
– based on noncoding regions of DNA that have repeating DNA sequences
– number of repeats differs between people
9.3 DNA Fingerprinting
A DNA fingerprint is NOT actually a fingerprint! XX
9.3 DNA Fingerprinting
Blood found at crime scene– Need to determine if it
is the suspect’s blood, or the victim’s blood
– We can use DNA fingerprinting to find out
– The DNA found at the crime scene belongs to the suspect
9.3 DNA Fingerprinting
Make a DNA fingerprint (restriction map) based on the DNA sequence repeats below (p. 16)
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9.3 DNA Fingerprinting
Make a DNA fingerprint (restriction map) based on the DNA sequence repeats below (p. 16)
• Read the Article• Watch the video and answer the questions
Who Killed Sally Parker?:
Gel ElectrophoresisA DNA Fingerprinting
Simulation
Today’s TaskToday you will work with a partner to perform a lab simulation based on a scenario in which you are to identify the murderer of Ms. Sally Parker.
This activity will model the processes of gel electrophoresis and DNA fingerprinting
Let’s get started!!
Procedures• Begin by reading the scenario and
discussing it with your partners. Be sure you understand the problem.
• Get the materials needed to complete the activity.
Procedures• Following base pairing rules, please fill in the complementary strand
of DNA
• Examine each DNA fragment strip closely, looking for the sequence AATT. This is a restriction site, where enzymes will cut the DNA into even smaller fragments. Use a pencil to mark these sites, with a line between the inner A and the inner T (AA│TT).
• Then use the scissors to cut across the fragments at those sites. Count and label the number of base pairs contained in each of the DNA fragments.
• Use the chart paper provided by your teacher to make a larger version of the gel electrophoresis chamber.
• Use a ruler or meter stick to make sure all of the lengths are the same.