8/3/2019 9. Culture Media
1/23
Culture Media
8/3/2019 9. Culture Media
2/23
Culture Media
is a solid or gel based
substance facilitating thegrowth of
micro organisms or tissues.
8/3/2019 9. Culture Media
3/23
Culture media can be usedin three forms
1.Solid2.Semisolid
3.Fluid
8/3/2019 9. Culture Media
4/23
Basic type of Culture
media
Bacterial Culture media.
Viral Culture media.
Tissue Culture media.
8/3/2019 9. Culture Media
5/23
Common ingredients of
Culture media
Peptone
Meat extracts
Yeast extracts Mineral salts
8/3/2019 9. Culture Media
6/23
Carbohydrates
Agar Water
8/3/2019 9. Culture Media
7/23
Main types of culture
media Basic
Enriched and enrichment Selective
Differential
Transport
8/3/2019 9. Culture Media
8/23
Basic media
a simple media such as
nutrient agar or broth
do not have specialnutritional requirements
8/3/2019 9. Culture Media
9/23
Is often used
1.in preparation of enrichedmedia
2.maintain stock culture ofcontrol strains of bacteria
3.For subculturingpathogens fromdifferential or selective
media
8/3/2019 9. Culture Media
10/23
Enriched media
provide additional nutrientsor growth stimulants
are required for culture of
1. H. influenzae
2. Pathogenic Neisseria
3. Several Streptococcusspecies
8/3/2019 9. Culture Media
11/23
are enriched with
whole blood
lyzed blood
serum
Extra peptones
Special extracts orvitamin
8/3/2019 9. Culture Media
12/23
Examples
1.Blood agar
2.Chocolate agar etc
8/3/2019 9. Culture Media
13/23
Enrichment media
Fluid medium that increasesthe numbers of a pathogen by
containing enrichments, andor substances that
discourage the multiplicationof unwanted bacteria.
8/3/2019 9. Culture Media
14/23
Example:
Selenite F broth is used anenrichment media for
Salmonellae in faeces orurine prior to subculturing
on xylose lysinedeoxycholate agar or other
enteric selective medium.
8/3/2019 9. Culture Media
15/23
Selective media
Facilitate the growth of
some pathogenicorganisms
Prevent or slow down the
growth of other organisms
8/3/2019 9. Culture Media
16/23
Examples
1. SS agar Facilitate growth of
salmonella and shigella Inhibite growth of
many faecalcommensals (bycontaining Bile salt)
8/3/2019 9. Culture Media
17/23
2.Modified New York City
(MNYC) medium Used to isolate
N. gonorrhoeaefromurethral specimens
8/3/2019 9. Culture Media
18/23
3. Butzler medium to isolate
Compylobacter sppfromfaeces
Available for isolating most
of important organisms
8/3/2019 9. Culture Media
19/23
Differential (indicator)
mediacontain some indicators,
dyes, other substanceshelp to differentiate
microorganisms
8/3/2019 9. Culture Media
20/23
Examples
1.TCBS agar Indicator bromothymol
blue Differentiate sucrose
fermenting from nonfermenting Vibrio spp
8/3/2019 9. Culture Media
21/23
8/3/2019 9. Culture Media
22/23
Transport media
Mostly a semisolid media
Prevent the overgrowth ofcimmensals
Ensure the survival of
aerobic and anaerobicpathogens
8/3/2019 9. Culture Media
23/23
Used to transport the
microbial specimens Examples
1.Amies transport medium
2.Cary-Blair medium