7.Chuong1. Tong Quan

Wedelia biflora (L.) DC Tng quan

HV: Nguyn Th Hoi Thu 1

Chng 1. TNG QUAN 1.1. C TNH THC VT

Trong cc cy h Cc (Asteraceae), gm 70 loi cy thuc chi Wedelia, trong mt s cy c bit n Vit Nam [1, 4, 5, 6] nh:

Sn cc hai hoa - Wedelia biflora (L.) DC. Sn cc nhm - Wedelia urticaefolia (Bl.) DC. Sn cc b - Wedelia chinensis (Osb.) Merr. Sn cc ba thy - Wedelia trilobata (L.) Hitch. Si t - Wedelia calendulacea L. L a cc - Wedelia prostrata (H. & A.) Hemls.

1.1.1. Cy sn cc hai hoa - Wedelia biflora (L.) DC. (Hnh 1.1a) Cy Sn cc hai hoa c tn khoa hc l Wedelia biflora (L.) DC. [1, 5]. Sn cc hai hoa cn c gi l cc mt tri, rau mui, hi cc. Dng cy bi, cao 1-2m. Thn c lng cng, tha. L c phin xoan xoan

tam gic, c t lng nhm nhm. Hoa u c c hay tng cp, trn cng di, hoa hnh mi vng, ci, 5-10, gia hoa c l hoa l vy. B qu khng c lng mo, cao 4mm (hnh 1.1b).

L li tiu, hoa x mnh. Cy mc n , Nam Trung Quc, Vit Nam, Thi Lan, Malaixia,

Innxia, Philippin, nc ta cy sn cc hai hoa c trng lm cnh trong cc cng vin thnh ph H Ch Minh, Lt 1.1.2. Sn cc nhm - Wedelia urticaefolia (Bl.) DC. (Hnh 1.2)

Sn cc nhm c tn khoa hc l Wedelia urticaefolia (Bl.) DC. [1, 5]. Sn cc nhm l dng cy c a nin ng. Thn c lng nm, nhm. L c

phin xoan nhn, di 6-12cm. Hoa u khng hay c cng, c vy, hoa hnh ba mi vng, ci; hoa gia hnh ng, lng phi. B qu dp, cao 1mm, khng lng mo.

Phn b n , Trung Quc, Vit Nam, Lo, Thi Lan, Innxia. nc ta, cy mc hoang rt nhiu hu ht cc tnh t Lo Cai, Cao Bng n Ninh Thun, Lm ng



1.1.3. Sn cc b - Wedelia chinensis (Osb.) Merr. (Hnh 1.3) Sn cc b c tn khoa hc l Wedelia chinensis (Osb.) Merr. [1]. Sn cc b cn c gi l sn cc Trung Quc. Dng cy c a nin b t, thn c lng phn. L c phin hp di 5-8 cm, rng 1 cm, c lng nhm, ba c rng nhn, tha. Hoa u c c trn cng di cht nhnh, rng 1,5 cm, hnh mi vng, hoa trong hnh ng, lng phi. B qu c a mng, c rng th lng mo. Thng phn b i ct k bin Vng Tu, Hu, Qui Nhn, 1.1.4. Sn cc ba thy - Wedelia trilobata (L.) Hitch. (Hnh 1.4) Sn cc ba thy c tn khoa hc l Wedelia trilobata (L.) Hitch.

Sn cc ba thy cn c tn l cc b. Cy sn cc ba thy l loi tho mc sng lu nm, c th mc thng cao

n 45 cm, hoc b lan n 1,8 m. Cy thng mc thnh tng mng, dy c che kn mt t. Thn b, r mc ti cc t, di khong 1-3 dm. L dy, di khong 4-9 cm, rng 2-5 cm c rng ca hai bn thy l, c lng cng v th c hai mt. Hoa mu vng ti, nhiu cnh thnh tng, cung hoa di 3-10 cm, tng bao c dng chung, u hoa thng c khong 8-13 hoa tia, a trng hoa di 4-5 mm, b qu c nt sn.

Cy c tm thy nhiu Trung M, Ty Phi, Hawai, Nam Florida, Vit Nam, cy mc hoang nhiu ni v c dng rng ri lm cy king. 1.1.5. Wedelia paludosa DC. (Hnh 1.5)

Cha thy c ti liu no cp n. 1.1.6. Si t - Wedelia calendulacea (L.) Less (Hnh 1.6) Si t c tn khoa hc l Wedelia calendulacea (L.) Less. [6]. Si t cn c gi l hng trm, ng ni, cc nhp, cc gip, bnh k cc. Si t l mt loi c sng dai, mc lan b, ch thn mc ti u, r lan ti , ni t tt c th cao 0,5m. Thn mu xanh, c lng trng, cng, nh. L gn nh khng cung, mc i, hnh bu dc thon di, hai u nhn, di 15-50 mm, rng 25 mm, c lng nh cng hai mt, mp c 1-3 rng ca nng. Cm hoa hnh



u, cung hoa di, vt qua cc nhnh l, cnh hoa mu vng ti. Qu b khng c lng, u thu hp li, tn cng mang mt vng c rng. Thng mc hoang nhiu tnh min bc, nhng ni m mt. 1.1.7. L a cc - Wedelia prostrata (H. & A.) Hemls (Hnh 1.7) L a cc c tn khoa hc l Wedelia prostrata (H. & A.) Hemls. [6]. L a cc cn c tn gi l bnh k cc.

Cy dng c a nin, b t, thn c lng phn. L c lng nhm tha, phin di 1-2 cm, ba c t rng. Hoa u c c, hnh mi vng, c vy gia cc hoa, hoa hnh ng, lng phi. B qu cao 1 mm, u c lng mn, khng lng mo. Thng c dng lm thuc mc tc, tr nhc u.

Phn b n , Trung Quc, Vit Nam, Lo, Thi Lan, Innxia. nc ta, cy mc hoang nhiu ni. Thng phn b vng i ct da bin Hu, Qui Nhn, Nha Trang, Vng Tu.



Hnh 1.1a: Ton cy Sn cc hai hoa - Wedelia biflora (L.) DC.

Hnh 1.1b: L, hoa, ht ca Wedelia biflora (L.) DC



Hnh 1.2: Wedelia urticaefolia (Bl) DC. Hnh 1.3: Wedelia chinensis (Osb.) Merr.

Hnh 1.4: Wedelia trilobata (L.) Hitch Hnh 1.5: Wedelia paludosa DC.

Hnh 1.6: Wedelia calendulacea (L.) Less Hnh 1.7: Wedelia prostrata (H. & A.) Hemls



1.2. THNH PHN HA HC 1.2.1. Cy sn cc hai hoa - Wedelia biflora (L.) DC.

Nm 1993, Miles D. Howard [20] c lp t cao diclorometan ca l kh Wedelia biflora c bn hp cht: veratrylidenehydrazid (59); 3,3'-di-O-metylquercetin (47); 2,7,4-trihydroxy-5,3-dimetoxyisoflavon (51) v 7,3-di-O-

metyl quercetin (48).

Nm 2005, Nguyn Thanh Hong v cng s [3] c lp t l cy Wedelia biflora c hai hp cht l: -amyrin acetat (36) v acid kaur-16-en-19-oic (28) v c lp t hoa cy Wedelia biflora c hai hp cht l: 3-hexanoyloxyolean-12-en-28-ol (38) v acid 3-hexanoyloxyolean-12-en-28-oic (39). 1.2.2. Cy sn cc nhm - Wedelia urticaefolia (Bl.) DC. Nm 2007, Nguyn Thanh Hong v cng s [2, 23] c lp c -amyrin (35); 3-acetoxy--amyrin (36), 3-O-tetradecanoylurs-12-en-16-ol (37); phytol (15) v acid kaur-16-en-19-oic (28) t hoa ca cy Wedelia urticaefolia. 1.2.3. Cy sn cc b - Wedelia chinensis (Osb.) Merr.

Nm 1986, Yang Ling Ling [34] c lp t Wedelia chinensis c acid kaur-16-en-19-oic (28) v stigmasterol glucosid (46).

Nm 2002, Apers Sandra v cng s [8] c lp t cy thuc Wedelia chinensis Trung Quc c acid 3,4-dicaffeoylquinic (57); acid 3,5-dicaffeoylquinic (56); acid 4,5-dicaffeoylquinic v ,3,4-trihydroxyphenyletyl-O-[-apiofuranosyl-(14)--rhamnopyranosyl-(13)]-(4-O-caffeoyl)--glucopyranosid hay wedelosin (58); cng lc vi ba flavonoid glycosid l: 3-O--glucopyranosylquercetin (54); 3-O--apiofuranosyl-(1-2)--glucopyranosylkaempferol (53) v astragalin hay 3-O--glucopyranosylkaempferol (52).

1.2.4. Cy sn cc ba thy - Wedelia trilobata (L.) Hitch. Nm 2000, Koheil M. A. v cng s [19] kho st thnh phn tinh du ca hoa

Wedelia trilobata bng GC-MS. Kt qu cho thy thnh phn ca tinh du gm 48 cht trong thnh phn chnh l -phellandren (25,65%); limonen (8,93%); -terpinen (5,90%); trans--caryophyllen (4,83%) v -pinen (4,72%).



Nm 2004, Zhang Yuhu [35] c lp c 6 sesquiterpen t cy Wedelia trilobata: trilobolid-6-O-isobutyrat hay wedeliatrilolactone A (8); 1-acetoxy-4,9-dihydroxy-6-isobutyroxyprostatolid (10); hn hp gm hai hp cht l 1,9-diacetyloxy-4-hydroxy-6-isobutyryloxyprostatolid hay wedeliatrilolactone B (9) v 1,9-diacetyloxy-4-hydroxy-6-metacryloxyprostatolid (12); 1,4-dihydroxy-6-isobutyryloxy-9-tigloyloxyprostatolid (13) v 9-angeloyloxy-1,4-dihydroxy-6- isobutyryloxyprostatolid (14).

Nm 2005, Nirmal v cng s [25] kho st thnh phn ha hc tinh du ca l Wedelia trilobata bng GC-MS. Kt qu cho thy tinh du cha 14 hp cht, trong -pinen; germacren D v d-limonen chim n 85,52%.

Nm 2006, Nguyn Thanh Hong v cng s [24] c lp t l ca Wedelia trilobata c friedelan-3-ol (34); -amyrin acetat (36) v acid 3-tigloyloxykaur-16-en-19-oic (25).

1.2.5. Wedelia paludosa DC.

Nm 1987, Roque Nidia F. [28] c lp t r Wedelia paludosa c: metyl 9-hydroxykaur-16-en-19-oat (21); metyl kaur-16-en-19-oat (22); metyl 3-angeloyloxy-9-hydroxykaur-16-en-19-oat (18); metyl 3-angeloyloxykaur-16-en-19-oat (19); metyl 3-tigloyloxykaur-16-en-19-oat (20); metyl 3-cinamoyloxykaur-16-en-19-oat (23); metyl 3-cinamoyloxy-9-hydroxykaur-16-en-19-oat (24); mt labdan l (1R,2R,8aS,Z)-2,5,5,8a-tetrametyl-1-(3-metylpenta-2,4-dienyl)-decahydronaptalen-2-ol (15); mt secokauren lacton l (1R,5S,8R)-1,5-dimetyl-8-{2-[(1S,5R)-6-metylen-2-

oxobicyclo[3.2.1]oct-1-yl]etyl}-6-oxabicyclo[3.2.1]octan-7-on (17).

Nm 2001, De Carvalho v cng s [12] c lp t hoa Wedelia paludosa c acid kaur-16-en-19-oic (28); acid kaur-9(11),16-dien-19-oic (27); acid 3-O-hexadecanoylolean-12-en-28-oic (40); acid 3-O-pentadecanoylolean-12-en-28-oic (41); acid 3-O-tetradecanoylolean-12-en-28-oic (42); stigmasterol (45); hn hp 3-O--D-glycopyranosylsitosterol v 3-O--D-glucopyranosylstigmasterol (46).

Nm 2003, Sartori M. R. K. v cng s [29] c lp t hoa Wedelia paludosa c hai hp cht l acid kaur-16-en-19-oic (28) v luteolin (49).



Nm 2004, Cechinel Filho Valdir v cng s [11] c lp t cy Wedelia paludosa c stigmasterol (45), acid kaur-16-en-19-oic (28) v acid oleanolic v eudesmanolid (4).

1.2.6. Si t - Wedelia calendulacea L. Nm 1991, Govindachari T. R. [16] c lp t l cy ti Wedelia

calendulacea c -D-glucopyranosyl 3-[(O--D-xylopyranosyl)-(12)-(-D- glucuronopyranosyl)]olean-12-en-28-oat (43) v -D-glucopyranosyl 3-[(O--D-glucopyranosyl)-(12)-(-D-glucuronopyranosyl)]olean-12-en-28-oat hay ginsenosid Ro (44).

Nm 1998, Nguyn Ngc Sng v cng s [22] c lp t l ti cy Wedelia calendulacea c mt sesquiterpen lacton l wedelolacton (6) v acid 5,6-dihydroxy-2-(2',4',6'-trihydroxyphenyl)benzofuran-3-carboxylic hay acid

norwedelic (7).

Nm 2003, Mottakin A. K. M. [21] v cng s c lp t Wedelia calendulacea c ba diterpen l: acid 3-tigloyloxykaur-16-en-19-oic (25); acid 3-angeloyloxykaur-16-en-19-oic (26) v acid kaur-16-en-19-oic (28). 1.2.7. L a cc - Wedelia prostrata (H. & A.) Hemls Nm 1993, Ragasa Consolacion Y. v cng s [27] c lp t cy Wedelia prostrata thu hi Philippines c 1-acetoxy-4,9-dihydroxy-6-metacryloxyprostatolid (11) v acid 3-cinamoyloxykaur-16-en-19-oic (32). Nm 2001, Farag Salwa F. [13] c lp t dch chit cloroform ca phn trn mt t ca cy Wedelia prostrata c 3-hydroxycarvotagenon (1); 3-hydroxycarvotagenon (2); p-ment-1-en-3,6-diol (3); acid 16,17-dihydroxykauran-19-oic (30) v 3-O--D-glucopyranosylkaempferol (52). Hn th na, tc gi cng c lp c t r ca cy ny c stigmasterol (45); acid 15,16-epoxy-17-hydroxykauran-19-oic (31); stigmasterol 3-O--D-glucopyranosid (46); quercetin-3-O--D-rutinosid (55); acid 3,5-dicaffeoylquinic (56) v acid 3,4-dicaffeoylquinic (57).



Cu trc ha hc ca cc cht c lp c t cc loi thuc chi Wedelia c trnh by theo cc loi hp cht nh sau: Monoterpen

O

OH

1

3

3-Hydroxycarvotagenon O

OH

1

3

3-Hydroxycarvotagenon OH

OH

1

3

p-Ment-1-en-3,6-diol(1) (2) (3)

Sesquiterpen

OH

CHO

HO

OO

Eudesmanolid

OH

2-[4-Metyl-3-(prop-1-en-2-yl)-4-vinylcyclohexyl]propan-2-ol

OHO

HOCOOH

HO OH

OH

Acid norwedelic

1

35

6 2OHO

HO O

1

35

6 2

O

HO OCH3

Wedelolacton(4) (5) (6) (7)

O O

O

O

HO

AcO OH

H

H

H

1

4

9

6

1-Acetoxy-4-dihydroxy-6-metacryloxyprostatolidWedeliatrilolacton BWedeliatrilolacton A

O O

O

O

HO

AcO OAc

H

H

H

O O

O

O

HO

AcO OAc

H

H

H

O O

O

O

HO

AcO OH

H

H

H

1

4 6

9

1-Acetoxy-4,9-dihydroxy-6-isobutyroxyprostatolid

(8) (9) (10) (11)

O O

O

O

HO

AcO OAc

H

H

H

1

4

9

6

1,9-Diacetoxy-4-hydroxy-6-metacryloxyprostatolid

O O

O

O

HO

HO O

H

H

H

1

4

9

6

O

1,4-Dihydroxy-6-isobutyryloxy-9-tigloyloxyprostatolid

O O

O

O

HO

HO O

H

H

H

1

4

9

6

O

9-Angeloyloxy-1,4-dihydroxy-6- isobutyryloxyprostatolid.

(12) (13) (14) Diterpen

HO

Phytol (15, Hoa Wedelia urticaefolia)



OO

O

(1R,5S,8R)-1,5-Dimetyl-8-{2-[(1S,5R)-6-metylen-2-oxobicyclo[3.2.1]oct-1-yl]etyl}

-6-oxabicyclo[3.2.1]octan-7-on

1

5

8

71'5'

6'

2'HO

(1R,2R,8aS,Z)-2,5,5,8a-Tetrametyl-1-(3-metylpenta-2,4-dienyl)-decahydronaptalen-2-ol

12

5

8a

(16) (17)

Metyl 3-angeloyloxy- 9-hydroxykaur-16-en-19-oat

H

OH

OH3CO

916

O

O

Metyl 3-angeloyloxykaur-16-en-19-oat

H

OH3CO

916

O

O

Metyl 3-tigloyloxykaur-16-en-19-oat

H

OH3CO

916

O

O3

Metyl 9-hydroxykaur-16-en-19-oat

H

OH

OH3CO

916

(18) (19) (20) (21)

19 19 19 19

Metyl kaur-16-en-19-oat

H

OH3CO

16

Metyl 3-cinamoyloxy-9-hydroxykaur-16-en-19-oat

H

OH

O

H3CO

9

16

O

O

Metyl 3-cinamoyloxykaur-16-en-19-oat

H

O

H3CO

916

O

O3

43

(22) (23) (24)

19 19 19

HOOCH

Acid 3-tigloyloxykaur-16-en-19-oic

O

O

HOOCH

Acid 3-angeloyloxykaur-16-en-19-oic

O

O

HOOCH

Acid kaura-9(11),16-dien-19-oic(25) (26) (27, Hoa Wedelia paludosa)

HOOCH

Acid kaur-16-en-19-oicHOOC

H

Acid 16-hydroxykauran-19-oic

OH

Acid 16,17-dihydroxykauran-19-oicHOOC

H

OHOH

(28, Hoa Wedelia paludosa,Hoa Wedelia urticaefolia)

(29) (30)

HOOCHO

O

Acid 3-cinamoyloxykaur-16-en-19-oic

H

Acid 10-hydroxy-9-metyl-20-norkaur-16-en-19-oic--lacton

OO

Acid 15,16-epoxy-17-hydroxykauran-19-oic

HOOCH

OOH

(31) (32) (33)



Triterpen

HO

Friedelan-3-ol

5

913

HO

-Amyrin(34) (35, Hoa Wedelia urticaefolia) (36, Hoa Wedelia urticaefolia))

O

-Acetoxy--amyrin

CH3C

O

3 3

1212

CH2OH

O

-Hexanoyloxyolean-12-en-28-olC

O

H3C(CH2)4O

-Tetradecanoyloxyurs-12-en-16-olC

O

H3C(CH2)12

OH

COOH

O

Acid -hexanoyloxyolean-12-en-28-oicC

O

H3C(CH2)4

(37, Hoa Wedelia urticaefolia) (38, Hoa Wedelia biflora) (39, Hoa Wedelia biflora)

3

28

1212

16

3

Acid 3-(hexadecanoyloxy)olean-12-en-28-oic

COOH

OC

O

H3C(CH2)14

Acid 3-(pentadecanoyloxy)olean-12-en-28-oic

(40, Hoa Wedelia paludosa)

Acid 3-(tetradecanoyloxy)olean-12-en-28-oic

COOH

OC

O

H3C(CH2)13

COOH

OC

O

H3C(CH2)12

(41, Hoa Wedelia paludosa) (42, Hoa Wedelia paludosa)

C

O

-D-Glucopyranosyl 3-[(O--D-xylopyranosyl)-(1 2)-(-D-glucuronopyranosyl)]olean-12-en-28-oat

OHO

HOOH

O

OH

O

OHO

HOO

COOH

OHO

HOOH

C

O

-D-glucopyranosyl 3-[(O--D-glucopyranosyl)-(1 2)-(-D-glucuronopyranosyl)]olean-12-en-28-oat

(Ginsenosid Ro), (44)

OHO

HOOH

O

OH

O

OHO

HOO

COOH

OHO

HOOH

OH

(43)

2



Sterol

O

Stigmasterol 3-O-D-glucopyranosid

OHOHO

OH

OH

HOStigmasterol

(45, Hoa Wedelia paludosa) (46, Hoa Wedelia paludosa)

Flavon

OH3CO

OH O

OCH3OH

7,3'-Di-O-metylquercetin

OH

OHO

OH O

OCH3OH

3,3'-Di-O-metylquercetin

OCH3

OHO

O

OH

OH

7,3',4'-Trihydroxyflavanon

OHO

OH O

OH

OH

Luteolin(47) (48) (49, Hoa Wedelia paludosa) (50)

3

3' 3'

7 7

3'

4'

OHO

OCH3 O

OH

OCH3

OH

2,7,4'-Trihydroxy-5,3'-dimetoxyisoflavon

OHO

O

OH

O

3-O--D-Glucopyranosylkaempferol

OHO

HOOH

OH

HO

OHO

O

OH

O

OHO

HOO

OH

HO

OH OH

O

3-O--D-Apiofuranosyl-(1 2)--D-glucopyranosylkaempferol

(51) (52) (53)

3

3

HO

(54)

OHO

OH O

OH

OH

O

Quercetin 3-rutinosid

OHO

HOOH

O

OHHO

HO

O

OHO

OH O

OH

OH

O

3-O--D-Glucopyranosylquercetin

OHO

HOOH

OH

(55)

3

3



Dn xut ca acid caffeic

O

OHO

OH

HOOC

OH

HO

O

OH

OH

O

Acid 3,5-dicaffeoylquinic

O

OOH

OH

HOOCOH

OH

O

Acid 3,4-dicaffeoylquinic

OH

O

OH

353

4

(56) (57)

211 2

HO

O

OO

OOH

OH

O

OHHO

O

OH OH

O

O

OH

OHHO

13

4

Wedelosin

HO

(58)

HO

Hydrazid

OCH3

H3CO

NN

OCH3

OCH3

O

Veratrylidenhydrazid

H

(59) 1.3. DC TNH HC 1.3.1. Cy sn cc hai hoa - Wedelia biflora (L.) DC. C Mau v nhiu ni khc [1], ngi dn dng t l non lm rau xo vi tht, c len, c, ra. L cy c dng lm thuc tr ni m ay bng cch ly ba nm l gi, vt ly nc ri pha vi ng hoc mui ung. n [1], l c gi ra, dng lm thuc p ln vng da b bin mu, p ln vt ct, vt su b t, lot, sng, gin tnh mch. L cn c dng p vo bng ph n sau khi sinh, sc ly nc ung tr i tiu ra mu. R dng tr bnh lu, si thn hoc dng p ln vt thng, gh nga. Thi Lan [1], ngi ta dng thn, l tr au u v st.



1.3.2. Sn cc nhm - Wedelia urticaefolia (Bl.) DC. Vn Nam (Trung Quc) [1], ngi ta s dng Wedelia urticaefolia lm thuc thanh nhit, gii c, b huyt v hot huyt tr vim phi v nhng tn thng do t ng. 1.3.3. Sn cc b - Wedelia chinensis (Osb.) Merr. Wedelia chinensis [4] c dng lm thuc h nhit, khng sinh, tr nhc u, tr bnh gan, tr ph bng, chng vim, tr ra mu t cung, tr bnh da, t thp. Trung Quc, c ni dng Wedelia chinensis tr ung th (Ehrlich ascite).

Nm 1986, Yang Ling Ling [34] c lp t Wedelia chinensis c acid kaur-16-en-19-oic v stigmasterol glucosid v chng minh rng hai hp cht ny c tc dng lm gim c tnh ca t bo gan chut b gy c bi CCl4 hoc D-galactosamin.

Nm 2005, Bai Xiang Yang [10] lm ra sn phm tr Wedelia chinensis bng cch phi trn phn thn v cung Wedelia chinensis vi cc dc phm b sung theo t l khi lng l 1:0,3. Tr c tc dng thanh nhit, gii c, kch thch tiu ha, tt cho bao t, l lch, gip hi phc ci thin sc khe. 1.3.4. Sn cc ba thy - Wedelia trilobata (L.) Hitch.

Nm 2004, Zhang Yuhu [35] chng minh rng 6 sesquiterpen c lp t cy Wedelia trilobata c hot tnh c ch s pht trin mm ca cy c ci, ci bp, c chua

Nm 2005, Baczynski v cng s [9] cho bit b tc Miskito ca n s dng Wedelia trilobata tr rn cn, vt thng do c ui gai c gy ra. Ngoi ra, cy cn cha c cc bnh nh: tiu chy, si thn, cm lnh

Nm 2005, Nirmal v cng s [25] cho bit tinh du ca l Wedelia trilobata c hot tnh khng khun ng k vi chng vi khun Bacillus subtilis v Staphylococcus aureus.

1.3.5. Wedelia paludosa DC.

Nm 2003, theo Sartori M. R. K. v cng s [29], ngi dn Brazin s dng Wedelia paludosa nh cy thuc truyn thng dng tr bnh nm ngoi da. Dch trch hexan, diclorometan and butanol ca hoa Wedelia paludosa th hin hot tnh



khng li cc chng nm gy bnh ngoi da nh: Epidermophyton floccosum, Trichophyton rubrum v Trichophyton mentagrophyte, vi nng t 250 n 1000 g/ml. Hai hp cht tinh khit l acid kaurenoic v luteolin cng cho thy c hot tnh khng nm ny. 1.3.6. Si t - Wedelia calendulacea (L.) Less.

Trn lm sng, cy si t biu hin hai tc dng r rt [6]: gim au, gim st, khng sinh r rt, khng thy c tnh. Nhn dn H Bc v nhiu ni khc dng cy Si t n sng nh rau vi tht c. Mt s ni dng Si t tr rm sy hoc ung phng si, cha st rt, vim ty ngoi da, khp xng, nhim trng, l lot, mn nht, au mt, vim bng quang

Nm 2004, Mottakin A. K. M. v cng s [21] th nghim hot tnh khng 19 chng vi khun v th nghim c tnh Brine Shrimp ca cc dch chit nh: eter du ha, cloroform, metanol v ca ba diterpen c lp c t cy Wedelia calendulacea. Kt qu th nghim c tnh Brine Shrimp cho thy cc dch chit c hot tnh tt vi liu LC50 l 4,59 g/ml (dch chit eter du ha), 7,99 g/ml (dch chit cloroform), 14,88 g/ml (dch chit metanol). Ring hp cht acid (-)-kaur-16-en-19-oic cho thy c hot tnh khng khun vi hu ht cc chng vi khun th nghim, vi liu 200 g/ a th ng knh vng v khun l 10-21 mm. 1.3.7. L a cc - Wedelia prostrata (H. & A.) Hemls

Nhn dn Trung Quc [6] dng cy L a cc ti tr vim amyan cp tnh, sng au c hng, vim phi, vim ph qun, ho lu ngy, cao huyt p, ho ra mu, chy mu cam, u mi. Dng cy L a cc ti cng vi dm cha bnh bch hu c kt qu 96,75%. L a cc phi kh, nu cao dng tiu c, cha vim ty, nht, p-xe, ho, l lot

7.Chuong1. Tong Quan

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