715004503EN_AflaM1V_ InsGuide_4_FINAL_062514

INSTRUC T ION GUIDE

2CULTIVATING SUCCESSTHROUGH SCIENCE

For more than 20 years, VICAM has been the global

provider of choice for next-generation food safety

technology and rapid mycotoxin test solutions.

The goal of securing the worlds food supply requires

knowledge, cooperation, and collaboration. To advance

that objective, VICAM works closely with government

regulatory agencies, international standards bodies,

major food industry laboratories, and leading research

institutions around the world. With a global scientific

and distribution presence, VICAM has access to the latest

research advances, industry expertise, and regulatory

information needed to design solutions that maximize

food safety and quality at every stage of the global food

supply chain.

As the trusted partner of the agricultural industry, VICAM

is proud to consistently deliver the superior products,

responsive service, and individualized support for your

industry-specific goals.

AflaM1-V Kit (25 Tests) .................................... 176003404

AFLA M1-V KIT CONTENTS

Afla M1-V Strip Test ......................................... 100000310

Afla M1-V Diluent Vials, 6 mL ......................... 100000308

Strip Test Pipettes ............................................ 600000812

Strip Test Vials, 1.5 mL ................................... 600000813

Afla M1-V Barcode Set.... ................................. 715004500

Syringe Disk Filters 5 m. ............................... 600001331

5 mL Syringe .................................................... 600001333

Afla M1-V Instruction Guide ............................. 715004503

Vertu Afla M1-V Basic Equipment Package with Printer, 110V .................................... 176003429

Vertu Afla M1-V Basic Equipment Package with Printer, 220V .................................... 176003430

EQUIPMENT PACKAGE CONTENTS

Vertu Reader with Barcode Scanner, 110V/220V ...................................................... 725000574

Afla M1-V Drawer for Vertu Reader .................. 600001332

Vortex Genie II, 110V ....................................... 600001219

Vortex Genie II, 220V ...................................... 600001220

Vertu Printer, 110V/220V ............................... 725000577

Strip Test Vial Rack .......................................... 600001107

Digital Timer .................................................... G4036

Strip Test Vials (25/pk) ................................... 600000813

Complete Mini Incubator 110V ........................ 176003201

Complete Mini Incubator 220V ....................... 176003202

Syringe Disk Filters, 5 m ............................... 600001331

5 mL Syringe .................................................... 600001333

4Dairy products such as milk, yoghurt and cheese provide essential nutrition to the human diet. The protein, fat, vitamins and minerals found in dairy products support healthy growth and development in children while also supporting the nutritional needs of adults and supporting heart health, bone strength and healthy weight maintenance. Milk is also believed to help fight cardiovascular disease, type 2 diabetes and osteoporosis while helping to meet daily requirements for human nourishment.

PRECISEQUANTITATIVEFAST

2

3Dairy feed is a critical requirement for dairy producers and has a direct impact on milk production and quality. If grain, feed or forage containing aflatoxin B1 is consumed by dairy cattle, is it then converted to aflatoxin M1 through normal metabolic processes and is expressed in the milk at a rate of 1-2%. The International Agency for Research on Cancer classifies aflatoxin M1 as a Group 2B (or probable) carcinogen. Global regulatory limits for aflatoxin in milk and other dairy products range from 0.5 parts per billion (ppb) in the US to as low as 0.05 ppb for the EU, with even lower limits for food intended for children. VICAM offers rapid, on-site test kits and laboratory solutions that deliver the precise data required to ensure that dairy products are both nutritious and safe to consume.

6AFLA M1-V PROCEDURE FOR RAW MILK

Set Up1. Turn on the Vertu reader by pressing the

center key hard. Using the arrow keys, move the curser to measure on the display, and then gently press the center key. You will then hear 3 beeps.

2. Calibrate the Vertu reader daily by scanning in the bar code for the lot of strips being used. You will hear a beep when the bar code is accepted. Make sure the lot number displayed on the reader is the same lot number as the strips.

3. Turn on the power for mini-incubator and pre-set temperature at 48 C. Let warm up for about 10 min until 48 C is on the display.

Sample Procedure1. Draw up 3-5 mL of cold (approximately

7 C) raw milk with syringe.

2. Attach the filter to the end of the syringe. Push sample through syringe filter and collect enough milk to run the test in a strip test vial. Not all the milk may go through the filter.

3. Add 250 L of filtered milk sample to the Afla M1-V diluent vial using a strip test pipettor* and mix well by vortexing 3 times for about 5 seconds each time.

4. Place the Afla M1-V strip into the vial and place vial in mini-incubator for 12 minutes.

Reading the Developed Strip1. After 12 minute incubation, insert the

developed Afla M1-V strip into the Vertu reader drawer (test lines facing up with logo to the right) and close drawer.

2. Press the center key on the reader to take a reading.

3. To print the result, move the cursor to P in the upper left hand corner of the display using the arrow keys then press the center key.

4. To run the next sample, move the cursor to NT using the arrow keys and press the center key. If you are using the same lot of strips, you are now ready to insert the next sample. If you are using a different lot of strips, you will need to scan in the new bar code.

* Strip Test Pipettor Use

1. Squeeze the top bulb of the pipettor.

2. Draw up enough liquid to fill the barrel of the pipettor and have one or two drops of liquid spill over into the lower bulb.

3. At this point the barrel of the pipettor should be completely full of liquid.

4. To ensure accurate measurement, be careful not to let any liquid drip out of the pipettor before dispensing.

5. Squeeze the upper bulb to dispense the liquid.

6. Squeeze all of the liquid out of the barrel when dispensing.

4

Strip tests must be refrigerated. Store at 4 - 8 C (39 - 46 F).

Strip test should be at room temperature before use.

Use in accordance with VICAMs testing procedure. To be used for testing food products only. Not to be ingested or applied to the skin.

AFLA M1-V PROCEDURE FOR RAW MILK

5

Notes1. When scanning in the bar code using the

bar code scanner, make sure the red + in the center of the bar code scanner is in the center of the bar code. You may need to move bar code scanner up and down to get to the correct position for scanning.

2. Press the center key gently and quickly.

Technical AssistanceFor assistance please contact your local distributor or Vicam Technical Services by phone at 800-338-4381 in the United States or e-mail to [email protected].

BENEFITS

Results in 12 minutes*

Simple No special

training required

Precise Limits of

detection as low as 25 ppt

Convenient Easily performed

onsite or in the lab

Accurate Real-time data

which can be printed or

downloaded to a computer

Wide Range - 0 to 750 ppt

*after filtration

2KEY LOCATIONS

Headquarters:

34 Maple StreetMilford, MA 01757USATel.: +1 800 338 4381 +1 508 482 4935Fax: +1 508 482 4972

Orders:1848 N. Deffer DriveNixa, MO 65714USATel.: +1 877 228 4244 +1 417 725 6588Fax: +1 417 725 6102

www.vicam.com

715004503 June 2014 SC-PDF

Subject to change without notice.

2014 Waters Corporation. Waters, The Science of Whats Possible and VICAM are registered trademarks of Waters Corporation. Cultivating Success Through Science and Afla M1-V are trademarks of Waters Corporation.The analytical methods presented in this data sheet have been researched and developed by VICAM to be used exclusively with Afla-M1 products. These analytical methods have been validated in the VICAM laboratories to perform to the specifications indicated in the Afla M1-V procedures. The user assumes all risk in using Afla M1-V analytical procedures and products. VICAM makes no warranty of any kind, expressed or implied, other than that Afla M1-V products conform to VICAMs printed specification and quality control standards. VICAM will, at its option, repair or replace any product, or part thereof, which proves to be defective in workmanship or material. VICAMs undertaking to repair or service such products is exclusive and is in lieu of all other warranties whether written, oral, expressed, or implied, including any implied warranty of merchantability or fitness for a particular purpose. VICAM shall have no liability for anticipated or lost profits or any loss, inconvenience or damage whether direct, indirect, incidental, consequential or otherwise, to person or property, or for strict liability or negligence arising from or in connection with the use of these assay procedures or Afla M1-V products.