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Problema Solución
E coli no es suficientemente robusta Usar bacterias ambientales
Resistencia a antibióticos como
marcador de selección
Marcadores no antibióticos y resistencia
extraíble
Funciones codificadas en plásmidos Integración estable en cromosoma
Expresión dependiente de inductoresquímicos
Expresión dependiente en inputambiental
Fuerte selección contra los genes/circuitos implantados
Ortogonalización de la función
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