563UN

RANDOX Laboratories Ltd., 55 Diamond Road, Crumlin, Co. Antrim, United Kingdom, BT29 4QY Tel:Tel:Tel:Tel: +44 (0) 28 9442 2413 Fax: +44 (0) 28 9445 2912 EmailEmailEmailEmail: [email protected] : [email protected] : [email protected] : [email protected] Website: www.randox.coWebsite: www.randox.coWebsite: www.randox.coWebsite: www.randox.commmm

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CATALOGUE NO.CATALOGUE NO.CATALOGUE NO.CATALOGUE NO. CAL 2350 Calibration Serum Level 2 (CAL 2) DESCRIPTION Calibration Serum. INTENDED USE For use as a Calibrator in clinical chemistry assays. RANDOX Calibration Sera are based on lyophilised human serum. The concentrations and activities are suitable for calibration of clinical chemistry assays on a wide range of automatic analysers. Constituent concentrations are available at 2 levels. SAFETY PRECAUTIONS AND WARNINGS Human source material from which this product has been derived has been tested at donor level for the Human Immunodeficiency Virus (HIV 1, HIV 2) antibody, Hepatitis B Surface Antigen (HbsAg), and Hepatitis C Virus (HCV) antibody and found to be NON-REACTIVE. FDA approved methods have been used to conduct these tests. However, since no method can offer complete assurance as to the absence of infectious agents, this material and all patient samples should be handled as though capable of transmitting infectious diseases and disposed of accordingly. For IN VITRO diagnostic use only. STORAGE AND STABILITY Unreconstituted serum is stable up to the expiry date shown on the side of each individual bottle. Once reconstituted, the components of the Calibration Sera are stable for 8 hours at +25°C, 7 days at +4°C and 1 month at -20°C when frozen once (see limitations). PREPARATION FOR USE Serum must only be reconstituted using the following procedure: 1. Open the vial carefully, avoiding any loss of material. 2. Reconstitute by pipetting exactly 5 ml of distilled water at +20 to +25°C, into the vial. 3. Replace the rubber stopper and leave to stand for 30 minutes out of bright light before use. 4. Swirl gently several times during the reconstitution period to ensure that the contents are completely dissolved. 5. Prior to use, mix the contents by inverting the vial. Do not shake the vial as the formation of foam should be avoided. Ensure that no lyophilised material remains unreconstituted. 6. The serum is then ready for use with either a manual test or with an automated instrument. MATERIALS PROVIDED Calibration Serum Cat No. CAL2350 20 x 5ml Level 2 MATERIALS REQUIRED BUT NOT PROVIDED Calibrated Pipette, double deionised water. LIMITATIONS After reconstitution, bicarbonate is stable for 8 hours in the closed bottle and 1 hour in the open bottle. For Total and Prostatic Acid Phosphatase, the material should be stabilised by adding 1 drop (25-30µl) of 0.7M Acetic acid solution to 1ml of the serum exactly 30 minutes after reconstitution. After stabilisation Total & Prostatic Acid Phosphatase are stable for 2 hours at +25°C, 2 days at +4°C and 1 month when frozen once at -20°C. Alkaline Phosphatase levels in the reconstituted serum will rise over the stability period. It is recommended that the reconstituted serum be allowed to stand for 1 hour at +25°C before measurement. Bilirubin in the serum is light sensitive and it is recommended that the serum be stored in the dark. Stored in the dark it is stable for 1 day at +4°C. Do not store at +15°C to +25°C. Do not freeze. Bacterial contamination of the reconstituted serum will cause reductions in the stability of many components. Different lot numbers of this calibrator should not be interchanged as the values assigned to the calibrators vary from lot to lot. VALUE ASSIGNMENT Each batch of serum is distributed to approximately 3000 laboratories worldwide and values are assigned by a consensus of results obtained by these laboratories. The Calibration values for each instrument have been determined in at least 10 independent laboratories. Values are verified against a master lot of calibrator which is traceable to reference methods or reference materials. In some cases values may be assigned at Randox Laboratories in comparison to a master lot of calibrator which is traceable to reference methods or reference materials. NOTES ® All trademarks recognised. (1) Values established by reference laboratories officially recognised by the Federal Chamber of Physicians in

Germany. (2) DGKC : German Society for Clinical Chemistry (3) IFCC : International Federation of Clinical Chemistry (4) SCE : Scandinavian Committee on Enzymes 14 Apr ‘09 ne

Albumin g/l 43.9 Bromocresol Green g/dl 4.39

Alkaline Phosphatase U/l 221 AMP optimised to IFCC 37°CU/l 205 AMP non-optimsed 37°C

ALT (GPT) U/l 44 Tris buffer no P5P IFCC/SFBC 37°CAST (GOT) U/l 35 Tris buffer no P5P IFCC/SFBC 37°CBilirubin Direct µmol/l 21.9 Diazo with Dichloroaniline (DCA)

mg/dl 1.28Bilirubin Total µmol/l 30.4 Diazo with Dichloroaniline (DCA)

mg/dl 1.78µmol/l 28.3 Oxidation to Biliverdin mg/dl 1.65

Calcium mmol/l 2.34 Arsenazo III mg/dl 9.38

Chloride mmol/l 102 ISE indirect Cholesterol mmol/l 4.21 Cholesterol Oxidase

mg/dl 163CK Total U/l 216 CK-NAC (IFCC) 37°CCreatinine µmol/l 137 Alkaline picrate no deproteinization

mg/dl 1.55µmol/l 141 Jaffe rate blanked mg/dl 1.60

gamma-GT U/I 57 Gamma glutamyl.-3-carboxy-4-nitroanilide 37°CU/I 60 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 37°C

Glucose mmol/l 6.39 Hexokinase mg/dl 115mmol/l 6.45 Glucose oxidase mg/dl 116

Iron µmol/l 18.6 Colorimetric without ppt. µg/dl 104

LD (LDH) U/l 212 L->P 37°CU/l 226 L->P IFCC 37°C

Lipase U/I 37 Roche Colorimetric 37°CMagnesium mmol/l 1.02 Arsenazo III

mg/dl 2.47mmol/l 1.01 Xylidyl Blue mg/dl 2.45

Phosphate Inorganic mmol/l 1.49 Phosphomolybdate UV mg/dl 4.62

CALIBRATION SERUM LEVEL 2 (CAL 2)ABBOTT AEROSET® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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RANDOX Laboratories Ltd., 55 Diamond Road, Crumlin, Co. Antrim, United Kingdom, BT29 4QYTel: +44 (0) 28 9442 2413 Fax: +44 (0) 28 9445 29122Email: [email protected] Website: www.randox.com

Potassium mmol/l 4.06 ISE method - indirect Protein Total g/l 61.5 Biuret reaction end point

g/dl 6.15Sodium mmol/l 144 ISE method - indirect Triglycerides mmol/l 1.18 Lipase/GPO-PAP no correction

mg/dl 104Urea mmol/l 7.78 Urease kinetic

mg/dl 46.8mmol/l 7.78 BUN mg/dl 21.8

Uric Acid (Urate) mmol/l 0.359 Uricase peroxidase no ascorbate oxidase mg/dl 6.03mmol/l 0.348 Uricase Peroxidase with ascorbate oxidase @ 546nm mg/dl 5.85

CALIBRATION SERUM LEVEL 2 (CAL 2)ABBOTT AEROSET® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Acid Phosphatase (Total) U/I 16.6 1-Naphthyl Phosphate substrate Kinetic 37°CAlbumin g/l 46.4 Bromocresol Green

g/dl 4.64g/l 45.1 Bromocresol Purple g/dl 4.51

Alkaline Phosphatase U/l 221 AMP optimised to IFCC 37°CU/l 218 AMP non-optimsed 37°C

ALT (GPT) U/l 43 Tris buffer no P5P IFCC/SFBC 37°CAmylase Total U/I 106 Abbott - blocked pNPG7 37°CAST (GOT) U/l 35 Tris buffer no P5P IFCC/SFBC 37°CBilirubin Direct µmol/l 24.1 Diazo with Sulphanilic Acid

mg/dl 1.41µmol/l 24.0 Diazo with Dichloroaniline (DCA) mg/dl 1.41

Bilirubin Total µmol/l 30.7 Diazo with Dichloroaniline (DCA) mg/dl 1.80µmol/l 30.7 Diazo with Sulphanilic Acid mg/dl 1.80µmol/l 31.1 Dichlorophenyl Diazonium (DPD) mg/dl 1.82µmol/l 29.4 Oxidation to Biliverdin mg/dl 1.72




mg/dl 1.56µmol/l 139 Jaffe rate blanked mg/dl 1.58

gamma-GT U/I 58 Gamma glutamyl.-3-carboxy-4-nitroanilide 37°CU/I 60 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 37°CU/I 58 DCL gamma glutamyl-3-carboxy-4-nitroanilide 37°C


CALIBRATION SERUM LEVEL 2 (CAL 2)Abbott Architect c8000/ci8200® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Iron µmol/l 18.6 Colorimetric with ppt. µg/dl 104µmol/l 18.6 Colorimetric without ppt. µg/dl 104

LD (LDH) U/l 217 L->P 37°CU/l 215 L->P IFCC 37°C

Lithium mmol/l 1.15 Spectrophotometric mg/dl 0.799

Magnesium mmol/l 0.986 Arsenazo III mg/dl 2.40



g/dl 6.15Sodium mmol/l 146 ISE method - indirect TIBC µmol/l 46.5 FE+UIBC(saturation with iron)

µg/dl 260Triglycerides mmol/l 1.19 Lipase/GPO-PAP no correction

mg/dl 105mmol/l 1.20 L/G Kinase EP. no correction mg/dl 106

Urea mmol/l 7.98 Urease kinetic mg/dl 48.0mmol/l 7.98 BUN mg/dl 22.3


CALIBRATION SERUM LEVEL 2 (CAL 2)Abbott Architect c8000/ci8200® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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ALT (GPT) U/l 48 Tris buffer no P5P IFCC/SFBC 37°CU/I 36 Tris buffer no P5P IFCC/SFBC 30°CU/I 27 Tris buffer no P5P IFCC/SFBC 25°C

AST (GOT) U/l 41 Tris buffer no P5P IFCC/SFBC 37°CU/I 28 Tris buffer no P5P IFCC/SFBC 30°CU/I 20 Tris buffer no P5P IFCC/SFBC 25°C

Bilirubin Direct µmol/l 22.2 Diazo with Dichloroaniline (DCA) mg/dl 1.30

Bilirubin Total µmol/l 27.3 Diazo with Dichloroaniline (DCA) mg/dl 1.60

Calcium mmol/l 2.33 Cresolphthalein complexone mg/dl 9.34

Chloride mmol/l 103 ISE direct Cholesterol mmol/l 4.43 Cholesterol Oxidase

mg/dl 171CK Total U/l 233 CK-NAC (IFCC) 37°C

U/I 146 CK-NAC (IFCC) 30°CU/I 99 CK-NAC (IFCC) 25°C

Creatinine µmol/l 134 Alkaline picrate no deproteinization mg/dl 1.51µmol/l 136 Jaffe rate blanked mg/dl 1.53

gamma-GT U/I 55 Gamma glutamyl.-3-carboxy-4-nitroanilide 37°CU/I 43 Gamma glutamyl.-3-carboxy-4-nitroanilide 30°CU/I 34 Gamma glutamyl.-3-carboxy-4-nitroanilide 25°CU/I 57 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 37°CU/I 45 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 30°CU/I 35 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 25°C

Glucose mmol/l 6.50 Glucose oxidase mg/dl 117


LD (LDH) U/l 438 P->L German methods 37°CU/I 316 P->L German methods 30°CU/I 222 P->L German methods 25°C

Magnesium mmol/l 0.909 Xylidyl Blue mg/dl 2.21

CALIBRATION SERUM LEVEL 2 (CAL 2)ABX Pentra 400® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Potassium mmol/l 4.11 ISE method - direct Protein Total g/l 63.6 Biuret reaction end point

g/dl 6.36Sodium mmol/l 142 ISE method - direct Triglycerides mmol/l 1.27 Lipase/GPO-PAP no correction

mg/dl 112Urea mmol/l 7.99 Urease end point

mg/dl 48.0mmol/l 7.86 Urease kinetic mg/dl 47.2mmol/l 7.86 BUN mg/dl 22.0

Uric Acid (Urate) mmol/l 0.353 Uricase peroxidase with ascorbate oxidase mg/dl 5.93mmol/l 0.352 Uricase Peroxidase with ascorbate oxidase @ 546nm mg/dl 5.91

CALIBRATION SERUM LEVEL 2 (CAL 2)ABX Pentra 400® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Bilirubin Direct µmol/l 20.9 Diazo with Sulphanilic Acid mg/dl 1.22

Bilirubin Total µmol/l 33.2 Diazo with Sulphanilic Acid mg/dl 1.94



mg/dl 161Creatinine µmol/l 143 Alkaline picrate no deproteinization

mg/dl 1.62gamma-GT U/I 51 Gamma glutamyl.-3-carboxy-4-nitroanilide 37°C

U/I 40 Gamma glutamyl.-3-carboxy-4-nitroanilide 30°CU/I 31 Gamma glutamyl.-3-carboxy-4-nitroanilide 25°CU/I 44 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 37°CU/I 35 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 30°CU/I 27 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 25°C


Iron µmol/l 17.3 Colorimetric without ppt. µg/dl 96.5



mg/dl 114Uric Acid (Urate) mmol/l 0.362 Uricase peroxidase no ascorbate oxidase

mg/dl 6.08

CALIBRATION SERUM LEVEL 2 (CAL 2)ALFA WASSERMANN ACE/spACE/NExCT® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Acid Phosphatase (Total) U/I 22.2 1-Naphthyl Phosphate, Kinetic with Pentane diol Activation 37°CAlbumin g/l 43.2 Bromocresol Green

g/dl 4.32g/l 39.0 Turbidimetric Assays g/dl 3.90

Alkaline Phosphatase U/l 187 Roche Integra AMP buffer 37°CU/I 146 Roche Integra AMP buffer 30°CU/I 119 Roche Integra AMP buffer 25°CU/l 186 AMP optimised to IFCC 37°CU/I 145 AMP optimised to IFCC 30°CU/I 119 AMP optimised to IFCC 25°C


Amylase Pancreatic U/I 75 Randox EPS Liquid and BM/Roche EPS Liquid 37°CAmylase Total U/I 98 Roche Integra 2-chloro-pNPG7 37°C

U/I 97 Roche liquid stable pNPG7 37°CAST (GOT) U/l 36 Tris buffer no P5P IFCC/SFBC 37°C

U/I 24 Tris buffer no P5P IFCC/SFBC 30°CU/I 17 Tris buffer no P5P IFCC/SFBC 25°C

Bicarbonate mmol/l 12.5 Enzymatic Bilirubin Direct µmol/l 18.7 Diazo with Sulphanilic Acid

mg/dl 1.09µmol/l 15.1 Roche JG factored mg/dl 0.883

Bilirubin Total µmol/l 30.3 Diazo with Sulphanilic Acid mg/dl 1.77µmol/l 30.2 Diazonium ion mg/dl 1.77



mg/dl 163CK Total U/l 213 CK-NAC substrate start (DGKC) 37°C

U/I 133 CK-NAC substrate start (DGKC) 30°CU/I 91 CK-NAC substrate start (DGKC) 25°CU/l 217 CK-NAC (IFCC) 37°CU/I 136 CK-NAC (IFCC) 30°CU/I 92 CK-NAC (IFCC) 25°C

CALIBRATION SERUM LEVEL 2 (CAL 2)COBAS INTEGRA® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Creatinine µmol/l 131 Alkaline picrate no deproteinization mg/dl 1.48µmol/l 137 Roche Creatinine Plus mg/dl 1.55µmol/l 129 Jaffe rate blanked mg/dl 1.46µmol/l 131 Jaffe rate blanked comp. (-26 µmol/l) mg/dl 1.48µmol/l 130 Jaffe rate blanked compensated (-18 µmol/l) mg/dl 1.47

gamma-GT U/I 54 Gamma glutamyl.-3-carboxy-4-nitroanilide 37°CU/I 43 Gamma glutamyl.-3-carboxy-4-nitroanilide 30°CU/I 33 Gamma glutamyl.-3-carboxy-4-nitroanilide 25°CU/I 58 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 37°CU/I 46 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 30°CU/I 36 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 25°C



LD (LDH) U/l 436 P->L German methods 37°CU/I 315 P->L German methods 30°CU/I 221 P->L German methods 25°CU/l 444 P->L SFBC 37°CU/I 321 P->L SFBC 30°CU/I 225 P->L SFBC 25°CU/l 231 L->P Roche/Randox 37°CU/I 167 L->P Roche/Randox 30°CU/I 117 L->P Roche/Randox 25°CU/l 229 L->P IFCC 37°CU/I 165 L->P IFCC 30°CU/I 116 L->P IFCC 25°C

Lipase U/I 38 Roche Colorimetric 37°CU/I 36 Roche Turbidimetric with colipase 37°C

Lithium mmol/l 1.07 Ion selective electrode mg/dl 0.743

Magnesium mmol/l 0.983 Chlorphosphonazo III mg/dl 2.39


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Osmolality mmol/kg 294 Calculated Phosphate Inorganic mmol/l 1.48 Phosphomolybdate enzymatic

mg/dl 4.59mmol/l 1.45 Phosphomolybdate UV mg/dl 4.50


g/dl 6.25g/l 62.8 Biuret reaction kinetic g/dl 6.28

Sodium mmol/l 144 ISE method - indirect TIBC µmol/l 45.9 FE+UIBC(saturation with iron)


mg/dl 108mmol/l 1.21 Lipase/GPO-PAP 0.11mmol/l correction mg/dl 107mmol/l 1.22 L/G Kinase EP. no correction mg/dl 108


Uric Acid (Urate) mmol/l 0.346 Uricase peroxidase with ascorbate oxidase mg/dl 5.81mmol/l 0.344 Uricase peroxidase no ascorbate oxidase mg/dl 5.78mmol/l 0.342 Uricase Peroxidase with ascorbate oxidase @ 546nm mg/dl 5.75


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Cholesterol mmol/l 4.33 Cholesterol Oxidase mg/dl 167

CK Total U/l 217 CK-NAC (IFCC) 37°CU/I 136 CK-NAC (IFCC) 30°CU/I 92 CK-NAC (IFCC) 25°C

gamma-GT U/I 53 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 37°CU/I 42 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 30°CU/I 33 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 25°C




Protein Total g/l 63.4 Biuret reaction end point g/dl 6.34

Triglycerides mmol/l 1.26 Lipase/GPO-PAP no correction mg/dl 112


CALIBRATION SERUM LEVEL 2 (CAL 2)COBAS MIRA® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Alkaline Phosphatase U/l 293 Diethanolamine buffer DEA 37°CU/I 228 Diethanolamine buffer DEA 30°CU/I 187 Diethanolamine buffer DEA 25°C




Calcium mmol/l 2.18 Cresolphthalein complexone mg/dl 8.74mmol/l 2.40 Arsenazo III mg/dl 9.62


CK Total U/l 236 CK-NAC serum start (DGKC) 37°CU/I 148 CK-NAC serum start (DGKC) 30°CU/I 100 CK-NAC serum start (DGKC) 25°CU/l 216 CK-NAC substrate start (DGKC) 37°CU/I 135 CK-NAC substrate start (DGKC) 30°CU/I 92 CK-NAC substrate start (DGKC) 25°C

Creatinine µmol/l 141 Alkaline picrate no deproteinization mg/dl 1.60

gamma-GT U/I 56 Gamma glutamyl.-3-carboxy-4-nitroanilide 37°CU/I 44 Gamma glutamyl.-3-carboxy-4-nitroanilide 30°CU/I 35 Gamma glutamyl.-3-carboxy-4-nitroanilide 25°C





CALIBRATION SERUM LEVEL 2 (CAL 2)FALCOR 300 series/TARGA® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Uric Acid (Urate) mmol/l 0.374 Uricase peroxidase no ascorbate oxidase mg/dl 6.28

CALIBRATION SERUM LEVEL 2 (CAL 2)FALCOR 300 series/TARGA® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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CALIBRATION SERUM LEVEL 2 (CAL 2)FALCOR 600/ALCYON® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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CALIBRATION SERUM LEVEL 2 (CAL 2)FALCOR 600/ALCYON® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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a-HBDH U/I 245 Oxobutyrate < 10 mmol/l 37°CU/I 185 Oxobutyrate < 10 mmol/l 30°CU/I 139 Oxobutyrate < 10 mmol/l 25°C

Acid Phosphatase (non-prostatic) U/I 4.20 1-Naphthyl Phosphate, Kinetic with Pentane diol Activation 37°CAcid Phosphatase (Prostatic) U/I 17.5 1-Naphthyl Phosphate, Kinetic with Pentane diol Activation 37°CAcid Phosphatase (Total) U/I 21.7 1-Naphthyl Phosphate, Kinetic with Pentane diol Activation 37°CAlbumin g/l 43.9 Bromocresol Green

g/dl 4.39g/l 45.4 Bromocresol Purple g/dl 4.54

Alkaline Phosphatase U/l 194 p-Nitrophenylphosphate AMP 37°CU/I 151 p-Nitrophenylphosphate AMP 30°CU/I 124 p-Nitrophenylphosphate AMP 25°CU/l 288 Diethanolamine buffer DEA 37°CU/I 224 Diethanolamine buffer DEA 30°CU/I 184 Diethanolamine buffer DEA 25°CU/l 182 AMP optimised to IFCC 37°CU/I 142 AMP optimised to IFCC 30°CU/I 116 AMP optimised to IFCC 25°C

ALT (GPT) U/l 46 Tris buffer no P5P IFCC/SFBC 37°CU/I 34 Tris buffer no P5P IFCC/SFBC 30°CU/I 26 Tris buffer no P5P IFCC/SFBC 25°CU/l 46 Phosphate buffer DGKC 37°CU/I 34 Phosphate buffer DGKC 30°CU/I 26 Phosphate buffer DGKC 25°C

Amylase Pancreatic U/I 74 Randox EPS Liquid and BM/Roche EPS Liquid 37°CAmylase Total U/I 93 Randox - Benzylidene pNPG7 37°C


U/I 27 Tris buffer no P5P IFCC/SFBC 30°CU/I 19 Tris buffer no P5P IFCC/SFBC 25°CU/l 43 Phosphate buffer DGKC 37°CU/I 29 Phosphate buffer DGKC 30°CU/I 20 Phosphate buffer DGKC 25°C

Bicarbonate mmol/l 12.8 Enzymatic Bilirubin Direct µmol/l 20.1 Dichlorophenyl Diazonium (DPD)

mg/dl 1.18µmol/l 20.7 Diazo with Sulphanilic Acid mg/dl 1.21

CALIBRATION SERUM LEVEL 2 (CAL 2)HITACHI SERIES® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Bilirubin Total µmol/l 29.7 Diazo with Dichloroaniline (DCA) mg/dl 1.74µmol/l 31.1 Diazo with Sulphanilic Acid mg/dl 1.82µmol/l 30.5 Dichlorophenyl Diazonium (DPD) mg/dl 1.78µmol/l 31.1 Diazonium ion mg/dl 1.82

Calcium mmol/l 2.36 Cresolphthalein complexone mg/dl 9.46mmol/l 2.31 Methylthymol blue mg/dl 9.26mmol/l 2.37 Arsenazo III mg/dl 9.50

Chloride mmol/l 99.5 ISE indirect Cholesterol mmol/l 4.25 Cholesterol Oxidase

mg/dl 164CK Total U/l 225 CK-NAC serum start (DGKC) 37°C

U/I 141 CK-NAC serum start (DGKC) 30°CU/I 96 CK-NAC serum start (DGKC) 25°CU/l 216 CK-NAC substrate start (DGKC) 37°CU/I 135 CK-NAC substrate start (DGKC) 30°CU/I 92 CK-NAC substrate start (DGKC) 25°CU/l 215 CK-NAC (IFCC) 37°CU/I 135 CK-NAC (IFCC) 30°CU/I 91 CK-NAC (IFCC) 25°C

Creatinine µmol/l 138 Alkaline picrate no deproteinization mg/dl 1.56µmol/l 149 Randox Enzymatic UV method mg/dl 1.68µmol/l 143 Roche Creatinine Plus mg/dl 1.61µmol/l 140 Jaffe rate blanked mg/dl 1.59µmol/l 139 Jaffe rate blanked comp. (-26 µmol/l) mg/dl 1.57

gamma-GT U/I 53 Gamma glutamyl.-3-carboxy-4-nitroanilide 37°CU/I 42 Gamma glutamyl.-3-carboxy-4-nitroanilide 30°CU/I 33 Gamma glutamyl.-3-carboxy-4-nitroanilide 25°CU/I 53 Gamma glutamyl-4-nitroanilide 37°CU/I 42 Gamma glutamyl-4-nitroanilide 30°CU/I 33 Gamma glutamyl-4-nitroanilide 25°CU/I 56 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 37°CU/I 44 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 30°CU/I 35 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 25°C


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gamma-GT U/I 62 Randox Gamma glutamyl.-3-carboxy-4-nitroanilide 37°CU/I 49 Randox Gamma glutamyl.-3-carboxy-4-nitroanilide 30°CU/I 38 Randox Gamma glutamyl.-3-carboxy-4-nitroanilide 25°C



LD (LDH) U/l 456 P->L German methods 37°CU/I 329 P->L German methods 30°CU/I 231 P->L German methods 25°CU/l 445 P->L SFBC 37°CU/I 321 P->L SFBC 30°CU/I 226 P->L SFBC 25°CU/l 225 L->P Roche/Randox 37°CU/I 162 L->P Roche/Randox 30°CU/I 114 L->P Roche/Randox 25°C

Lipase U/I 37 Roche Colorimetric 37°CMagnesium mmol/l 1.00 Xylidyl Blue

mg/dl 2.43Phosphate Inorganic mmol/l 1.49 Phosphomolybdate enzymatic



g/dl 6.38g/l 63.5 Biuret reaction kinetic g/dl 6.35

Sodium mmol/l 147 ISE method - indirect TIBC µmol/l 48.9 Removal of excess free iron

µg/dl 273µmol/l 43.5 FE+UIBC(saturation with iron) µg/dl 243

Triglycerides mmol/l 1.25 Lipase/GPO-PAP no correction mg/dl 111mmol/l 1.25 Lipase/GPO-PAP 0.11mmol/l correction mg/dl 111


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Triglycerides mmol/l 1.26 L/G Kinase EP. no correction mg/dl 112

Urea mmol/l 8.10 Urease end point mg/dl 48.7mmol/l 8.29 Urease kinetic mg/dl 49.8mmol/l 8.29 BUN mg/dl 23.2

Uric Acid (Urate) mmol/l 0.340 Uricase catalase 340nm mg/dl 5.71mmol/l 0.347 Uricase peroxidase with ascorbate oxidase mg/dl 5.83mmol/l 0.348 Uricase peroxidase no ascorbate oxidase mg/dl 5.85mmol/l 0.345 Uricase Peroxidase with ascorbate oxidase @ 546nm mg/dl 5.80


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Alkaline Phosphatase U/l 294 Diethanolamine buffer DEA 37°CU/I 229 Diethanolamine buffer DEA 30°CU/I 188 Diethanolamine buffer DEA 25°C
















CALIBRATION SERUM LEVEL 2 (CAL 2)ILab 300® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Alkaline Phosphatase U/l 227 AMP optimised to IFCC 37°CU/I 177 AMP optimised to IFCC 30°CU/I 145 AMP optimised to IFCC 25°C


Amylase Total U/I 100 I.L. - blocked pNPG7 37°CAST (GOT) U/l 33 Tris buffer no P5P IFCC/SFBC 37°C















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mg/dl 112Urea mmol/l 7.71 Urease end point




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Albumin g/l 41.0 Bromocresol Green g/dl 4.10g/l 41.5 Turbidimetric Assays g/dl 4.15

Alkaline Phosphatase U/l 206 p-Nitrophenylphosphate AMP 37°CU/I 160 p-Nitrophenylphosphate AMP 30°CU/I 132 p-Nitrophenylphosphate AMP 25°CU/l 305 Diethanolamine buffer DEA 37°CU/I 238 Diethanolamine buffer DEA 30°CU/I 195 Diethanolamine buffer DEA 25°CU/l 219 AMP optimised to IFCC 37°CU/I 171 AMP optimised to IFCC 30°CU/I 140 AMP optimised to IFCC 25°CU/l 229 AMP optimised to NVKC/SFBC 37°CU/I 178 AMP optimised to NVKC/SFBC 30°CU/I 146 AMP optimised to NVKC/SFBC 25°C


Amylase Total U/I 95 bioMerieux - blocked pNPG7 37°CU/I 88 bioMerieux 2-chloro-pNPG3 37°C


Bilirubin Total µmol/l 33.6 Diazo with Sulphanilic Acid mg/dl 1.97µmol/l 30.2 Nitrobenzenediazonium salt mg/dl 1.77






CALIBRATION SERUM LEVEL 2 (CAL 2)Konelab 20/30/60® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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gamma-GT U/I 60 Gamma glutamyl.-3-carboxy-4-nitroanilide 37°CU/I 47 Gamma glutamyl.-3-carboxy-4-nitroanilide 30°CU/I 37 Gamma glutamyl.-3-carboxy-4-nitroanilide 25°CU/I 45 Gamma glutamyl-4-nitroanilide 37°CU/I 35 Gamma glutamyl-4-nitroanilide 30°CU/I 28 Gamma glutamyl-4-nitroanilide 25°CU/I 61 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 37°CU/I 48 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 30°CU/I 38 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 25°C




Lipase U/I 39 Other Colorimetric 37°CMagnesium mmol/l 1.02 Arsenazo III

mg/dl 2.49mmol/l 0.983 Calmagite mg/dl 2.39mmol/l 0.946 Xylidyl Blue mg/dl 2.30






Uric Acid (Urate) mmol/l 0.369 Uricase peroxidase with ascorbate oxidase mg/dl 6.20


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a-HBDH U/I 229 Oxobutyrate < 10 mmol/l 37°CU/I 176 Oxobutyrate < 10 mmol/l 30°CU/I 142 Oxobutyrate < 10 mmol/l 25°C

Acid Phosphatase (non-prostatic) U/I 6.10 1-Naphthyl Phosphate substrate Kinetic 37°CU/I 3.60 1-Naphthyl Phosphate, Kinetic with Pentane diol Activation 37°C

Acid Phosphatase (Prostatic) U/I 10.5 1-Naphthyl Phosphate substrate Kinetic 37°CU/I 17.5 1-Naphthyl Phosphate, Kinetic with Pentane diol Activation 37°C

Acid Phosphatase (Total) U/I 16.6 1-Naphthyl Phosphate substrate Kinetic 37°CU/I 21.1 1-Naphthyl Phosphate, Kinetic with Pentane diol Activation 37°C

Albumin g/l 43.1 Bromocresol Green g/dl 4.31g/l 44.6 Bromocresol Purple g/dl 4.46g/l 41.1 Turbidimetric Assays g/dl 4.11

Alkaline Phosphatase U/l 210 p-Nitrophenylphosphate AMP 37°CU/I 164 p-Nitrophenylphosphate AMP 30°CU/I 134 p-Nitrophenylphosphate AMP 25°CU/l 301 Diethanolamine buffer DEA 37°CU/I 234 Diethanolamine buffer DEA 30°CU/I 192 Diethanolamine buffer DEA 25°CU/l 216 AMP optimised to IFCC 37°CU/I 168 AMP optimised to IFCC 30°CU/I 138 AMP optimised to IFCC 25°CU/l 225 AMP optimised to NVKC/SFBC 37°CU/I 175 AMP optimised to NVKC/SFBC 30°CU/I 144 AMP optimised to NVKC/SFBC 25°C

ALT (GPT) U/l 41 Tris buffer with P5P IFCC/SFBC 37°CU/I 30 Tris buffer with P5P IFCC/SFBC 30°CU/I 23 Tris buffer with P5P IFCC/SFBC 25°CU/l 44 Tris buffer no P5P IFCC/SFBC 37°CU/I 33 Tris buffer no P5P IFCC/SFBC 30°CU/I 25 Tris buffer no P5P IFCC/SFBC 25°CU/l 45 Phosphate buffer DGKC 37°CU/I 33 Phosphate buffer DGKC 30°CU/I 25 Phosphate buffer DGKC 25°CU/l 42 Tris buffer SCE 37°CU/I 31 Tris buffer SCE 30°CU/I 24 Tris buffer SCE 25°C

CALIBRATION SERUM LEVEL 2 (CAL 2)MEAN OF ALL INSTRUMENTS Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Amylase Pancreatic U/I 75 Roche liquid pNPG7 37°CU/I 85 Randox EPS Liquid 37°C

Amylase Total U/I 105 pNP Maltotrioside substrates 37°CU/I 106 Abbott - blocked pNPG7 37°CU/I 96 Siemens - blocked pNPG7 37°CU/I 94 bioMerieux - blocked pNPG7 37°CU/I 101 I.L. - blocked pNPG7 37°CU/I 88 Randox - Benzylidene pNPG7 37°CU/I 83 Randox - Ethylidene pNPG7 37°CU/I 111 Randox liquid stable pNPG7 37°CU/I 94 Beckman maltotetraose 37°CU/I 103 Siemens - maltopenta/hexaoside 37°CU/I 98 Roche Integra 2-chloro-pNPG7 37°CU/I 98 Roche liquid stable pNPG7 37°CU/I 102 Siemens 2-chloro-pNPG3 37°CU/I 88 bioMerieux 2-chloro-pNPG3 37°CU/I 97 Olympus - blocked pNPG7 37°C

AST (GOT) U/l 48 Tris buffer with P5P IFCC/SFBC 37°CU/I 32 Tris buffer with P5P IFCC/SFBC 30°CU/I 23 Tris buffer with P5P IFCC/SFBC 25°CU/l 37 Tris buffer no P5P IFCC/SFBC 37°CU/I 25 Tris buffer no P5P IFCC/SFBC 30°CU/I 18 Tris buffer no P5P IFCC/SFBC 25°CU/l 37 Phosphate buffer DGKC 37°CU/I 25 Phosphate buffer DGKC 30°CU/I 18 Phosphate buffer DGKC 25°CU/l 35 Tris buffer SCE 37°CU/I 24 Tris buffer SCE 30°CU/I 17 Tris buffer SCE 25°C

Bicarbonate mmol/l 13.2 Differential rate pH change mmol/l 13.0 Enzymatic mmol/l 13.2 Ion selective electrode

Bile Acids µmol/l 31.3 4th Generation Colorimetric µmol/l 28.1 5th Generation Colorimetric

Bilirubin Direct µmol/l 22.3 Dichlorophenyl Diazonium (DPD) mg/dl 1.30µmol/l 22.8 Diazo with Sulphanilic Acid mg/dl 1.33µmol/l 14.9 Roche JG factored mg/dl 0.869µmol/l 18.8 Oxidation to Biliverdin mg/dl 1.10µmol/l 15.9 Modified Jendrassik mg/dl 0.930


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Bilirubin Total µmol/l 32.9 Diazo with Dichloroaniline (DCA) mg/dl 1.92µmol/l 30.7 Diazo with Sulphanilic Acid mg/dl 1.80µmol/l 30.8 Dichlorophenyl Diazonium (DPD) mg/dl 1.80µmol/l 30.2 Nitrobenzenediazonium salt mg/dl 1.77µmol/l 30.5 Diazonium ion mg/dl 1.79µmol/l 28.9 Oxidation to Biliverdin mg/dl 1.69µmol/l 33.2 Modified Jendrassik mg/dl 1.94

Calcium mmol/l 2.33 Cresolphthalein complexone mg/dl 9.34mmol/l 2.31 Ion selective electrode mg/dl 9.26mmol/l 2.36 Methylthymol blue mg/dl 9.46mmol/l 2.38 Arsenazo III mg/dl 9.54

Chloride mmol/l 102 Colorimetric mmol/l 101 ISE indirect mmol/l 102 ISE direct


Cholinesterase U/l 5551 Colorimetric Butyrylthiocholine 37°CCK Total U/l 227 CK-NAC serum start (DGKC) 37°C

U/I 142 CK-NAC serum start (DGKC) 30°CU/I 96 CK-NAC serum start (DGKC) 25°CU/l 220 CK-NAC substrate start (DGKC) 37°CU/I 138 CK-NAC substrate start (DGKC) 30°CU/I 94 CK-NAC substrate start (DGKC) 25°CU/l 221 CK-NAC (IFCC) 37°CU/I 138 CK-NAC (IFCC) 30°CU/I 94 CK-NAC (IFCC) 25°CU/l 219 Monothioglycerol 37°CU/I 137 Monothioglycerol 30°CU/I 93 Monothioglycerol 25°CU/l 215 Dithioerythritol 37°CU/I 135 Dithioerythritol 30°CU/I 91 Dithioerythritol 25°C


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CK Total U/l 227 Dithioerythritol (DTE) IFCC correlated 37°CU/I 142 Dithioerythritol (DTE) IFCC correlated 30°CU/I 96 Dithioerythritol (DTE) IFCC correlated 25°C

Copper µmol/l 19.0 Atomic absorption µg/dl 121µmol/l 18.7 Colorimetric µg/dl 119

Creatinine µmol/l 142 Alkaline picrate with deproteinization mg/dl 1.60µmol/l 139 Alkaline picrate no deproteinization mg/dl 1.58µmol/l 146 Randox Enzymatic UV method mg/dl 1.65µmol/l 144 Creatinine PAP method mg/dl 1.63µmol/l 141 Roche Creatinine Plus mg/dl 1.59µmol/l 142 Jaffe rate blanked mg/dl 1.60µmol/l 138 Jaffe rate blanked comp. (-26 µmol/l) mg/dl 1.56µmol/l 131 Jaffe rate blanked compensated (-18 µmol/l) mg/dl 1.48

gamma-GT U/I 55 Gamma glutamyl.-3-carboxy-4-nitroanilide 37°CU/I 43 Gamma glutamyl.-3-carboxy-4-nitroanilide 30°CU/I 34 Gamma glutamyl.-3-carboxy-4-nitroanilide 25°CU/I 52 Gamma glutamyl-4-nitroanilide 37°CU/I 41 Gamma glutamyl-4-nitroanilide 30°CU/I 32 Gamma glutamyl-4-nitroanilide 25°CU/I 59 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 37°CU/I 46 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 30°CU/I 36 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 25°CU/I 56 DCL gamma glutamyl-3-carboxy-4-nitroanilide 37°CU/I 44 DCL gamma glutamyl-3-carboxy-4-nitroanilide 30°CU/I 35 DCL gamma glutamyl-3-carboxy-4-nitroanilide 25°CU/I 62 Randox Gamma glutamyl.-3-carboxy-4-nitroanilide 37°CU/I 49 Randox Gamma glutamyl.-3-carboxy-4-nitroanilide 30°CU/I 38 Randox Gamma glutamyl.-3-carboxy-4-nitroanilide 25°C

GLDH U/I 16 Triethanolamine buffer 50 mmol 37°CU/I 12 Triethanolamine buffer 50 mmol 30°CU/I 10 Triethanolamine buffer 50 mmol 25°C

Glucose mmol/l 6.42 Glucose dehydrogenase mg/dl 116


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Glucose mmol/l 6.36 Hexokinase mg/dl 115mmol/l 6.35 Oxygen electrode mg/dl 114mmol/l 6.54 Glucose oxidase mg/dl 118


Lactate mmol/l 1.57 Enzymatic Colorimetric mg/dl 14.1

LD (LDH) U/l 204 L->P 37°CU/I 147 L->P 30°CU/I 103 L->P 25°CU/l 492 P->L Scandinavian & Dutch 37°CU/I 355 P->L Scandinavian & Dutch 30°CU/I 249 P->L Scandinavian & Dutch 25°CU/l 448 P->L German methods 37°CU/I 323 P->L German methods 30°CU/I 227 P->L German methods 25°CU/l 453 P->L SFBC 37°CU/I 327 P->L SFBC 30°CU/I 230 P->L SFBC 25°CU/l 228 L->P Roche/Randox 37°CU/I 165 L->P Roche/Randox 30°CU/I 116 L->P Roche/Randox 25°CU/l 223 L->P IFCC 37°CU/I 161 L->P IFCC 30°CU/I 113 L->P IFCC 25°C

Lipase U/I 214 Siemens Dimension Colorimetric 37°CU/I 37 Roche Colorimetric 37°CU/I 46 Randox Colorimetric 37°C

Lithium mmol/l 1.08 Flame photometry mg/dl 0.749mmol/l 1.05 Ion selective electrode mg/dl 0.731mmol/l 1.08 Spectrophotometric mg/dl 0.751

Magnesium mmol/l 0.991 Arsenazo III mg/dl 2.41mmol/l 1.00 Calmagite mg/dl 2.43


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Magnesium mmol/l 0.983 Xylidyl Blue mg/dl 2.39mmol/l 0.910 Methylthymol blue mg/dl 2.21mmol/l 0.990 Chlorphosphonazo III mg/dl 2.41

Osmolality mmol/kg 295 Calculated mmol/kg 315 Freezing point depression

Phosphate Inorganic mmol/l 1.48 Phosphomolybdate enzymatic mg/dl 4.59mmol/l 1.47 Phosphomolybdate UV mg/dl 4.56

Potassium mmol/l 4.22 Enzymatic mmol/l 4.22 Flame photometry mmol/l 4.17 ISE method - direct mmol/l 4.18 ISE method - indirect

Protein Total g/l 61.0 Biuret reaction CX4/5/7 g/dl 6.10g/l 62.3 Biuret reaction end point g/dl 6.23g/l 61.0 Biuret reaction kinetic g/dl 6.10

Sodium mmol/l 145 Enzymatic mmol/l 146 Flame photometry mmol/l 144 ISE method - direct mmol/l 145 ISE method - indirect

TIBC µmol/l 46.6 Removal of excess free iron µg/dl 260µmol/l 46.0 FE+UIBC(saturation with iron) µg/dl 257µmol/l 50.6 Randox Direct µg/dl 283

Triglycerides mmol/l 1.24 Lipase/GPO-PAP no correction mg/dl 110mmol/l 1.24 Lipase/GPO-PAP 0.11mmol/l correction mg/dl 110mmol/l 1.22 L/G Kinase EP. no correction mg/dl 108mmol/l 1.21 L/G kinase EP. 0.11 mmol/l correction mg/dl 107mmol/l 1.22 Lipase/Glycerol Dehydrogenase mg/dl 108


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Urea mmol/l 7.98 Beckman-Conductivity mg/dl 48.0mmol/l 7.89 Urease end point mg/dl 47.4mmol/l 8.00 Urease kinetic mg/dl 48.1mmol/l 7.37 Urease hypochlorite mg/dl 44.3mmol/l 8.00 BUN mg/dl 22.5

Uric Acid (Urate) mmol/l 0.340 Uricase catalase 340nm mg/dl 5.71mmol/l 0.346 Uricase peroxidase with ascorbate oxidase mg/dl 5.81mmol/l 0.349 Uricase peroxidase no ascorbate oxidase mg/dl 5.86mmol/l 0.348 Spectrophotometric at 280-290 mg/dl 5.85mmol/l 0.359 Uricase Peroxidase with ascorbate oxidase @ 546nm mg/dl 6.03

Zinc µmol/l 30.1 Atomic absorption µg/dl 197µmol/l 31.6 Colorimetric with deproteinisation µg/dl 206


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a-HBDH U/I 237 Oxobutyrate < 10 mmol/l 37°CAcid Phosphatase (Total) U/I 20.0 1-Naphthyl Phosphate, Kinetic with Pentane diol Activation 37°CAlbumin g/l 42.6 Bromocresol Green

g/dl 4.26Alkaline Phosphatase U/l 238 p-Nitrophenylphosphate AMP 37°C

U/l 327 Diethanolamine buffer DEA 37°CU/l 238 AMP optimised to IFCC 37°C

ALT (GPT) U/l 44 Tris buffer no P5P IFCC/SFBC 37°CAmylase Total U/I 97 Olympus - blocked pNPG7 37°CAST (GOT) U/l 38 Tris buffer no P5P IFCC/SFBC 37°CBicarbonate mmol/l 12.6 Enzymatic Bilirubin Direct µmol/l 22.4 Dichlorophenyl Diazonium (DPD)

mg/dl 1.31µmol/l 21.3 Diazo with Sulphanilic Acid mg/dl 1.25

Bilirubin Total µmol/l 31.0 Diazo with Dichloroaniline (DCA) mg/dl 1.81µmol/l 30.4 Diazo with Sulphanilic Acid mg/dl 1.78µmol/l 31.5 Dichlorophenyl Diazonium (DPD) mg/dl 1.85




U/l 230 CK-NAC substrate start (DGKC) 37°CU/l 236 CK-NAC (IFCC) 37°C

Copper µmol/l 18.7 Colorimetric µg/dl 119



CALIBRATION SERUM LEVEL 2 (CAL 2)OLYMPUS AU400/500/600/800® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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LD (LDH) U/l 228 L->P 37°CU/l 511 P->L Scandinavian & Dutch 37°CU/l 436 P->L German methods 37°CU/l 227 L->P IFCC 37°C

Lipase U/I 38 Other Colorimetric 37°CMagnesium mmol/l 0.966 Xylidyl Blue









CALIBRATION SERUM LEVEL 2 (CAL 2)OLYMPUS AU400/500/600/800® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Alkaline Phosphatase U/l 190 Roche Integra AMP buffer 37°CU/I 148 Roche Integra AMP buffer 30°CU/I 121 Roche Integra AMP buffer 25°CU/l 189 AMP optimised to IFCC 37°CU/I 147 AMP optimised to IFCC 30°CU/I 121 AMP optimised to IFCC 25°C


Amylase Pancreatic U/I 75 Randox EPS Liquid and BM/Roche EPS Liquid 37°CAmylase Total U/I 98 Roche Integra 2-chloro-pNPG7 37°C



Bicarbonate mmol/l 13.0 Enzymatic Bilirubin Direct µmol/l 13.8 Diazo with Sulphanilic Acid

mg/dl 0.810Bilirubin Total µmol/l 27.9 Diazo with Sulphanilic Acid

mg/dl 1.63µmol/l 28.5 Dichlorophenyl Diazonium (DPD) mg/dl 1.67





Creatinine µmol/l 135 Alkaline picrate no deproteinization mg/dl 1.53µmol/l 135 Jaffe rate blanked mg/dl 1.52µmol/l 136 Jaffe rate blanked comp. (-26 µmol/l) mg/dl 1.53

gamma-GT U/I 54 Gamma glutamyl.-3-carboxy-4-nitroanilide 37°CU/I 43 Gamma glutamyl.-3-carboxy-4-nitroanilide 30°CU/I 33 Gamma glutamyl.-3-carboxy-4-nitroanilide 25°C

CALIBRATION SERUM LEVEL 2 (CAL 2)Roche Cobas 6000 c501 e601 Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Glucose mmol/l 6.37 Hexokinase mg/dl 115


LD (LDH) U/l 448 P->L German methods 37°CU/I 323 P->L German methods 30°CU/I 227 P->L German methods 25°CU/l 224 L->P Roche/Randox 37°CU/I 162 L->P Roche/Randox 30°CU/I 114 L->P Roche/Randox 25°C

Lipase U/I 37 Roche Colorimetric 37°CMagnesium mmol/l 1.01 Chlorphosphonazo III

mg/dl 2.46Phosphate Inorganic mmol/l 1.49 Phosphomolybdate UV

mg/dl 4.62Potassium mmol/l 4.17 ISE method - indirect Protein Total g/l 64.1 Biuret reaction end point






CALIBRATION SERUM LEVEL 2 (CAL 2)Roche Cobas 6000 c501 e601 Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Alkaline Phosphatase U/l 183 Roche Integra AMP buffer 37°CU/I 143 Roche Integra AMP buffer 30°CU/I 117 Roche Integra AMP buffer 25°C


Amylase Total U/I 98 Roche liquid stable pNPG7 37°CAST (GOT) U/l 36 Tris buffer no P5P IFCC/SFBC 37°C







Creatinine µmol/l 137 Roche Creatinine Plus mg/dl 1.55



Magnesium mmol/l 1.00 Chlorphosphonazo III mg/dl 2.43




CALIBRATION SERUM LEVEL 2 (CAL 2)Roche Cobas C111® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Uric Acid (Urate) mmol/l 0.334 Uricase peroxidase with ascorbate oxidase mg/dl 5.61mmol/l 0.346 Uricase peroxidase no ascorbate oxidase mg/dl 5.81

CALIBRATION SERUM LEVEL 2 (CAL 2)Roche Cobas C111® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Alkaline Phosphatase U/l 292 Diethanolamine buffer DEA 37°CU/l 216 AMP optimised to IFCC 37°C

ALT (GPT) U/l 43 Tris buffer no P5P IFCC/SFBC 37°CAmylase Total U/I 96 Siemens - blocked pNPG7 37°CAST (GOT) U/l 38 Tris buffer no P5P IFCC/SFBC 37°CBicarbonate mmol/l 13.7 Enzymatic Bilirubin Direct µmol/l 18.6 Oxidation to Biliverdin

mg/dl 1.09Bilirubin Total µmol/l 30.0 Diazo with Sulphanilic Acid

mg/dl 1.75µmol/l 29.6 Dichlorophenyl Diazonium (DPD) mg/dl 1.73µmol/l 28.8 Oxidation to Biliverdin mg/dl 1.68




U/l 226 CK-NAC substrate start (DGKC) 37°CU/l 215 CK-NAC (IFCC) 37°C

Creatinine µmol/l 147 Alkaline picrate no deproteinization mg/dl 1.67µmol/l 146 Jaffe rate blanked mg/dl 1.64µmol/l 144 Jaffe rate blanked comp. (-26 µmol/l) mg/dl 1.63




CALIBRATION SERUM LEVEL 2 (CAL 2)SIEMENS ADVIA 1650® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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LD (LDH) U/l 222 L->P 37°CU/l 430 P->L German methods 37°CU/l 230 L->P IFCC 37°C








CALIBRATION SERUM LEVEL 2 (CAL 2)SIEMENS ADVIA 1650® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Alkaline Phosphatase U/l 197 AMP optimised to IFCC 37°CU/I 153 AMP optimised to IFCC 30°CU/I 126 AMP optimised to IFCC 25°C

Bilirubin Direct µmol/l 22.0 Dichlorophenyl Diazonium (DPD) mg/dl 1.29









CALIBRATION SERUM LEVEL 2 (CAL 2)SIEMENS/CIBA 550 Express® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Albumin g/l 43.8 Bromocresol Green g/dl 4.38g/l 45.6 Bromocresol Purple g/dl 4.56

Alkaline Phosphatase U/l 170 Siemens Dimension AMP buffer 37°CALT (GPT) U/l 56 Siemens Dimension IFCC 37°CAmylase Total U/I 103 Siemens - maltopenta/hexaoside 37°C

U/I 102 Siemens 2-chloro-pNPG3 37°CAST (GOT) U/l 49 Siemens Dimension IFCC 37°CBicarbonate mmol/l 14.1 Enzymatic Bilirubin Direct µmol/l 15.0 Diazo with Sulphanilic Acid

mg/dl 0.878µmol/l 15.5 Diazo with Dichloroaniline (DCA) mg/dl 0.908

Bilirubin Total µmol/l 28.8 Diazo with Dichloroaniline (DCA) mg/dl 1.68µmol/l 28.8 Diazo with Sulphanilic Acid mg/dl 1.69



mg/dl 144mmol/l 3.72 Dimension-Siemens reagents mg/dl 144

CK Total U/l 224 CK-NAC serum start (DGKC) 37°CU/l 211 CK-NAC (IFCC) 37°CU/l 215 Dithioerythritol 37°CU/l 226 Dithioerythritol (DTE) IFCC correlated 37°C


gamma-GT U/I 68 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 37°CU/I 74 Siemens Dimension (IFCC) 37°C


CALIBRATION SERUM LEVEL 2 (CAL 2)SIEMENS DIMENSION® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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LD (LDH) U/l 194 Siemens Dimension L-P Non IFCC 37°CU/l 216 L->P IFCC 37°C

Lipase U/I 214 Siemens Dimension Colorimetric 37°CLithium mmol/l 1.07 Spectrophotometric

mg/dl 0.743Magnesium mmol/l 0.907 Methylthymol blue





mg/dl 107mmol/l 1.20 L/G Kinase EP. no correction mg/dl 106mmol/l 1.22 Lipase/Glycerol Dehydrogenase mg/dl 108

Urea mmol/l 7.58 Urease end point mg/dl 45.6mmol/l 7.82 Urease kinetic mg/dl 47.0mmol/l 7.82 BUN mg/dl 21.9

Uric Acid (Urate) mmol/l 0.344 Uricase catalase 340nm mg/dl 5.78mmol/l 0.352 Uricase peroxidase no ascorbate oxidase mg/dl 5.91mmol/l 0.348 Spectrophotometric at 280-290 mg/dl 5.85

CALIBRATION SERUM LEVEL 2 (CAL 2)SIEMENS DIMENSION® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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Albumin g/l 43.8 Bromocresol Green g/dl 4.38g/l 44.0 Bromocresol Purple g/dl 4.40

Alkaline Phosphatase U/l 209 p-Nitrophenylphosphate AMP 37°CU/I 163 p-Nitrophenylphosphate AMP 30°CU/l 206 AMP optimised to IFCC 37°CU/I 160 AMP optimised to IFCC 30°CU/l 210 AMP non-optimsed 37°CU/I 164 AMP non-optimsed 30°C

ALT (GPT) U/l 42 Tris buffer no P5P IFCC/SFBC 37°CU/I 31 Tris buffer no P5P IFCC/SFBC 30°CU/l 42 Tris buffer SCE 37°CU/I 31 Tris buffer SCE 30°C

Amylase Total U/I 94 Beckman maltotetraose 37°CU/I 98 Roche liquid stable pNPG7 37°C

AST (GOT) U/l 34 Tris buffer no P5P IFCC/SFBC 37°CU/I 23 Tris buffer no P5P IFCC/SFBC 30°CU/l 35 Tris buffer SCE 37°CU/I 24 Tris buffer SCE 30°C

Bicarbonate mmol/l 13.1 Differential rate pH change mmol/l 12.9 Ion selective electrode



Calcium mmol/l 2.30 Ion selective electrode mg/dl 9.22mmol/l 2.34 Arsenazo III mg/dl 9.38



U/I 144 CK-NAC serum start (DGKC) 30°CU/l 225 CK-NAC substrate start (DGKC) 37°CU/I 141 CK-NAC substrate start (DGKC) 30°CU/l 228 CK-NAC (IFCC) 37°CU/I 143 CK-NAC (IFCC) 30°C

CALIBRATION SERUM LEVEL 2 (CAL 2)SYNCHRON CX4/5/7/9/LX20® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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CK Total U/l 219 Monothioglycerol 37°CU/I 137 Monothioglycerol 30°C

Creatinine µmol/l 139 Alkaline picrate with deproteinization mg/dl 1.57µmol/l 143 Alkaline picrate no deproteinization mg/dl 1.61µmol/l 147 Jaffe rate blanked mg/dl 1.66

gamma-GT U/I 53 Gamma glutamyl.-3-carboxy-4-nitroanilide 37°CU/I 42 Gamma glutamyl.-3-carboxy-4-nitroanilide 30°CU/I 52 Gamma glutamyl-4-nitroanilide 37°CU/I 41 Gamma glutamyl-4-nitroanilide 30°CU/I 52 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 37°CU/I 41 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 30°C

Glucose mmol/l 6.25 GOD/02-Beckman method mg/dl 113mmol/l 6.32 Hexokinase mg/dl 114mmol/l 6.35 Oxygen electrode mg/dl 114mmol/l 6.35 Glucose oxidase mg/dl 114


LD (LDH) U/l 186 L->P 37°CU/I 134 L->P 30°CU/l 554 Pyruvate 1.4 mM - Beckman LD-P 37°CU/I 400 Pyruvate 1.4 mM - Beckman LD-P 30°CU/l 215 L->P Roche/Randox 37°CU/I 155 L->P Roche/Randox 30°C

Lithium mmol/l 1.02 Spectrophotometric mg/dl 0.705

Magnesium mmol/l 1.00 Calmagite mg/dl 2.44

Osmolality mmol/kg 293 Calculated Phosphate Inorganic mmol/l 1.52 Phosphomolybdate enzymatic

mg/dl 4.71mmol/l 1.47 Phosphomolybdate UV mg/dl 4.56mmol/l 1.64 Beckman PHOSm (365nm) mg/dl 5.08

Potassium mmol/l 4.17 ISE method - indirect Protein Total g/l 60.8 Biuret reaction CX4/5/7

g/dl 6.08


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Protein Total g/l 60.8 Biuret reaction end point g/dl 6.08g/l 59.8 Biuret reaction kinetic g/dl 5.98

Sodium mmol/l 145 ISE method - indirect TIBC µmol/l 45.8 Removal of excess free iron

µg/dl 256µmol/l 47.3 FE+UIBC(saturation with iron) µg/dl 264

Triglycerides mmol/l 1.25 Lipase/GPO-PAP no correction mg/dl 111mmol/l 1.20 L/G Kinase EP. no correction mg/dl 106

Urea mmol/l 8.00 Beckman-Conductivity mg/dl 48.1mmol/l 8.16 Urease kinetic mg/dl 49.0mmol/l 8.16 BUN mg/dl 22.8

Uric Acid (Urate) mmol/l 0.337 Uricase peroxidase with ascorbate oxidase mg/dl 5.66mmol/l 0.340 Uricase peroxidase no ascorbate oxidase mg/dl 5.71


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Alkaline Phosphatase U/l 204 AMP optimised to IFCC 37°CALT (GPT) U/l 46 Tris buffer no P5P IFCC/SFBC 37°CAST (GOT) U/l 37 Tris buffer no P5P IFCC/SFBC 37°CBilirubin Direct µmol/l 23.3 Dichlorophenyl Diazonium (DPD)

mg/dl 1.36Bilirubin Total µmol/l 30.3 Dichlorophenyl Diazonium (DPD)

mg/dl 1.77Calcium mmol/l 2.38 Arsenazo III

mg/dl 9.54Cholesterol mmol/l 4.14 Cholesterol Oxidase


mg/dl 1.52gamma-GT U/I 57 Gamma Glutamyl-3-Carboxy-4-nitroanilide (IFCC) 37°CGlucose mmol/l 6.45 Glucose oxidase

mg/dl 116LD (LDH) U/l 454 P->L SFBC 37°CPhosphate Inorganic mmol/l 1.52 Phosphomolybdate UV

mg/dl 4.71Protein Total g/l 62.0 Biuret reaction end point

g/dl 6.20Triglycerides mmol/l 1.25 Lipase/GPO-PAP no correction




CALIBRATION SERUM LEVEL 2 (CAL 2)VITALAB SELECTRA® Lot. No. 563UN Cat. No. CAL2350Size 20 x 5ml Expiry 2010-08Analyte unit target methods

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563UN

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use serum

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abccalibration serum

lyophilised human serum

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