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    Automatic Microplate Washer

    DROP

    USER MANUAL

    Software Version 2.2

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    DROP Automatic Microplate WasherUSER MANUAL

    Software Version 2.2

    SEAC S.R.L.Via di Prato, 72/74 - Calenzano Firenze (ITALY)

    Tel: +39 055 8877469 - Fax: +39 055 8877771

    Code 53872593

    Revision Date11/09/08

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    INDEX

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    1 GENERAL DESCRIPTION......................................................................................1-1

    1.1 Introduction ..........................................................................................................1-1

    1.2 Technical features................................................................................................1-2

    1.3 General characteristics ........................................................................................1-2

    1.4 Operation principles .............................................................................................1-3

    1.5 Precautions and restrictions.................................................................................1-4

    1.5.1 Use ..............................................................................................................1-4

    1.5.2 Performance................................................................................................1-4

    1.5.3 Protection.....................................................................................................1-4

    2 INSTALLATION AND TESTING .............................................................................2-1

    2.1 Introduction ..........................................................................................................2-1

    2.2 Transportation, storage and unpacking................................................................2-1

    2.2.1 Composition of the supply............................................................................2-2

    2.3 Preparation of the installation site ........................................................................2-3

    2.4 Correct installation of the instrument....................................................................2-3

    2.5 AC Power connection and grounding...................................................................2-4

    2.6 Environmental characteristics of the installation room ......................................... 2-4

    2.7 Installation............................................................................................................2-5

    2.8 Tests to be performed with the user.....................................................................2-6

    2.9 Disinstallation.......................................................................................................2-6

    3 USE .........................................................................................................................3-1

    3.1 Principal buttons...................................................................................................3-1

    3.2 Functions selection ..............................................................................................3-1

    3.2.1 Selection of washing microplate program ....................................................3-2

    3.2.2 Set the row number of washing microplate..................................................3-2

    3.2.3 Set the times of washing microplate ............................................................3-2

    3.2.4 Set immersion time of washing microplate...................................................3-3

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    3.2.5 Set the injection solution volume of washing microplate..............................3-3

    3.2.6 Align the position of washing tip...................................................................3-4

    3.2.7 Mode of shaking microplate.........................................................................3-4

    3.2.8 Mode of washing microplate ........................................................................3-5

    3.2.9 Adjust the level of suction solution...............................................................3-5

    3.2.10 Adjust the level of injection solution ............................................................. 3-5

    3.2.11 Adjust the middle position of two-point washing microplate

    (mode 2 of washing microplate)...................................................................3-6

    3.2.12 Adjust the special treatment for the last washing microplate ....................... 3-6

    3.2.13 Adjust the velocity of injection solution or suction solution...........................3-7

    3.2.14 Rinsing hose................................................................................................3-7

    4 MAINTENANCE AND TROUBLESHOOTING........................................................ 4-1

    4.1 Daily maintenance................................................................................................4-1

    4.1.1 Cleaning the outside of the instrument.........................................................4-1

    4.2 Troubleshooting ...................................................................................................4-2

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    INDEX OF FIGURES

    Figure 1.1 - Front View ...................................................................................................1-1

    Figure 1.2 - Block diagram..............................................................................................1-3

    Figure 2.1 - Sketch map..................................................................................................2-5

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    YOUR OPINION COUNTS FOR US

    Reference: DROP Automatic Microplate Washer - User Manual Code 53872592

    Your suggestions, advice and criticism are very welcome and useful for improving thismanual. We would therefore be grateful if you could answer the following questions:

    - Do you find this manual well arranged and easy to understand and follow? Please

    give us your impressions

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    - Have you found any mistakes in the instructions? Please indicate the paragraphand page.

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    COMPANY...............................................................................................................YOUR FULL NAME................................................................................................................ADDRESS..............................................................................................................................TELEPHONE..................................................................DATE..............................................

    Please return to:SEAC S.R.L. - Via di Prato, 72/74 - Calenzano (FI) ITALY.

    Thank you for your cooperation.

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    I. RESPONSIBILITY DECLARATION

    Our Company declines all responsibility for damage due to any type of modifications madeon the Hardware and Software due to connection to other instruments not carried out byour personnel, or not previously authorized by our firm.In case the apparatus is damaged do not switch it on until it has been repaired by atechnician from our company's Service Department.Any type of electric operation required to install or repair the instrument must be carriedout by our staff; do not install in any other way.The instrument should be used under the environment conditions and according to safetynorms given in points II and III of the following chapter, in order to guarantee that theresults obtained by the instrument are compliant with those given in the technicalspecifications.

    Use this instrument only after reading this manual

    II. SAFETY PRECAUTIONS

    The DROP Automatic Microplate Washer guarantees the maximum safety to the operatorduring its operation. However, as there is dangerous internal voltage, the operator mustfollow the general safety rules:

    Make sure that the Power Supply has the required voltage before switching on theinstrument

    The instrument must have a ground connection. In case of an electric short circuit theground connection eliminates the danger of electric contact with the external metalparts.

    In case of a current surge (due to a short circuit or another malfunction within theinstrument or due to sudden line voltage variations) the protection fuse located on theback will intervene; in this case, if the new fuse burns out again after having beenreplaced with another of the same kind, the instrument must be disconnected and theService Technicians contacted.

    Do not use the commands or adjustments inside the instrument and notify the servicestaff as soon as any anomaly occurs during functioning.

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    Before starting any type of intervention inside the instrument switch it off anddisconnect the power cable. Switch the instrument off from the Main Switch. (Press Oto switch off). Unplug the power cable to disconnect it; do not pull the cable.

    Any repairs to the instrument must be carried out by the Technical Assistance staff.

    Any electric operation that is necessary for the installation must be carried out byqualified staff.

    If the instrument is damaged do not switch it on until it has been repaired by atechnician of the Factory Assistance Service.

    Disconnect the instrument if it is left inactive for a long period.

    III. ELECTRICAL FEATURES AND LINE DISTURBANCE

    Disturbances on the power line may be caused by several factors such as:

    - Starting and stopping of electric motors.- Atmospheric phenomena.- Connection and disconnection of high line loads.

    It must be stressed how serious power supply line disturbances have negative effects onthe system.

    An efficient ground connection is necessary to reduce line disturbances and guarantee themaximum safety to the operator.

    Distribution : 1 phase, neutral and ground

    Power supply : 100 240 VAC

    50 60 Hz

    Power Consumption : 100 VA

    Line Fuse : 2 x T 0,6A

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    SYMBOL TABLE

    Serial Symbol Description

    1 Alternate current

    2 Earthing terminal

    3 On (power supply)

    4 Off (power supply)

    5 Fuse

    6 Warning! Risk of electric discharge.

    7 Warning! See instructions manuals for reference.

    8 Biological danger

    9 CE mark

    10 Waste for electronic device

    11 Part in movement

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    1 GENERAL DESCRIPTION

    1.1 INTRODUCTION

    Model DROP Automatic Microplate Washer is designed to wash the microplate forsatisfying the need of diagnosis in the medical field with reference to modern high-techprinciple. It is controlled by computer so that its operation is simple and fast and itswashing program can be easily configured. It can automatically and precisely complete thewashing from single row to the whole plate. On the base of its refined structure, reliablequality and good service after sale, Model DROP Automatic Microplate Washer will play an

    important role in assay laboratory.

    Figure 1.1 - Front View

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    1.2 TECHNICAL FEATURES

    Adjust the position of washing tip according to users need

    Set various washing parameters to complete different washing;

    Soak time : 5s-1200s; Adjustable with 5s step length

    Residual volume of washing solution : < than 1ul/well

    Washing times : 1-20 times-optional according to differentneeds

    Washing row : 1-12 rows-optional according to differentneeds

    Models of microplate applicable : both 48-well and 96-well microplate

    1.3 GENERAL CHARACTERISTICS

    Distribution : 1 phase, neutral and ground

    Power Supply : 100 240 VAC

    50 60 Hz

    Power Consumption : 100 VA

    Line fuse : 2 x T 0,6A

    Operating Conditions

    Temperature : 10 40 C

    Relative Humidity : 80%

    Atmosphere pressure : 860hPa-1060hPa.(84.86kPa-104.6kPa)

    Altitude : < 2.000 m (in funzione)

    Environmental coefficient : 2

    Sonic Pressure :

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    Safety : DIRECTIVE 73/23 CEE e 93/68 CEE

    Electromagnetic compatibility : DIRECTIVE 89/336 CEE

    In Vitro Diagnostic : DIRECTIVE 98/79/CE

    Dimension : 40 x37 x17 (h) cm

    Weight : 10 Kg

    1.4 OPERATION PRINCIPLES

    After receiving a washing signal from the keyboard, SCM will send a working signal topump and a pulse to drive circuit of step motor. With the progressing of step motor and theopening of valve, microplate washing starts according to the mode pre-selected.Communication between man and machine is realized through keyboard and LCD.

    Figure 1.2 - Block diagram

    KB

    SCM

    Step Motor Driver

    Step Motor Driver

    Washing Solution

    Bottle Pump Waste SolutionBottle

    Step Motor

    StepMotorCleaning Tip Device

    Valve

    Display

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    1.5 PRECAUTIONS AND RESTRICTIONS

    1.5.1 USE

    Model DROP Automatic Microplate Washer is used to wash the microplate in the assaylab of various medical units.

    The use of clinical samples implies an important biological risk andshould be carried out with extreme caution.Therefore, before handling test microplate, wear the appropriate

    protection gloves and face mask. In case of any leakage of fluids fromthe instrument, clean with diluted hypochlorite, always wearing theappropriate protection gloves and face mask.Tank emptying operations must also be carried out while wearing theappropriate protection gloves and face mask to prevent anycontamination due to contact or via aerosol.

    1.5.2 PERFORMANCE

    Performance standards are applicable only if the instrument has been properly used and

    maintained, as indicated in the Manual and using the reagents specified.

    The operator is strictly prohibited from changing the instruments internalsettings, unless explicitly instructed otherwise. Moreover, the operator mustreport any faults or anomalies observed during operation to the qualifiedtechnical staff.

    1.5.3 PROTECTION

    Electronic components may cause electric shock and injury. Do not removecovers or doors if not specifically recommended in this manual.

    The use of clinical samples involves an important biological risk and musttherefore be carried out with the utmost caution.

    The instrument is designed to ensure maximum protection of the user duringnormal operation.

    Always keep the panel closed during normal operation.

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    2 INSTALLATION AND TESTING

    2.1 INTRODUCTION

    The paragraphs below illustrate the procedures to follow for correct installation of theDROP Automatic Microplate Washer. This information concerns: instructions for transportation, storage and unpacking the instrument; reference documents for installation; procedures and particular requisites for installation; test procedure, designed to ascertain that the unit has been installed correctly and is

    able to carry out its function.

    2.2 TRANSPORTATION, STORAGE AND UNPACKING

    There are particular precautions to be observed in the transportation and storage and inunpacking the equipment to prevent it from being damaged or deterioration due toprolonged storage in an unsuitable area.

    TRANSPORTATION

    In its packaging the instrument weighs about 12 Kg and about 10 Kg without packaging.The dimensions are 49 x 46 x 35 (h) cm with packaging, and 40 x 37 x 17 (h) cm without.No special equipment is therefore required for transporting, loading or unloading theinstrument in its box.When handling the equipment the warning on the outside of the packaging (TOP-BOTTOM) must be respected.

    STORAGE

    The instrument must be kept in its original packing consisting of wood cases and stored inobservance of the TOP-BOTTOM signs marked on the outside.In setting the crate down, provide enough room for any handling and removal, as well asany necessary inspections.If the packing has been damaged during storage or shipment, it will at least be necessaryto verify that the instrument has no visible damage; the packing must be repaired beforestoring the instrument.If any damage is found, please contact the Manufacturer.The type of packing used does not guarantee protection or isolation should the crate be

    stored in a damp place; the storage room must be dry and free of dust.

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    UNPACKING and HANDLING

    Unpacking the instrument is a simple operation. Unpack with care and keep the packingmaterials in case the instrument has to be shipped back. One people are needed to carrythe instrument. Pick it up at the sides placing the hands near the center.Keep the instrument vertical during handling.

    WARNING: DO NOT TURN THE INSTRUMENT UPSIDE DOWN FOR ANY REASON.

    2.2.1 COMPOSITION OF THE SUPPLY

    The following items are supplied with the DROP Automatic Microplate Washer:

    Q.ty Description Observation/Code

    1 Power cable DIRECTIVEIEC60245/602270.75mm2, 3 Conductors withliners

    2 Fuse tube 250VAC,F,0.63A,520

    1 Clean solution bottle

    1 Waste solution bottle

    1 Buffer bottle

    5 Clean needle

    1 Set Dust sheet

    1 8 needles washing manifold

    1 12 needles washing manifold

    1 User Manual

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    2.3 PREPARATION OF THE INSTALLATION SITE

    The operating requirements are extremely important in the choice of the room where theinstrument is to be installed. In any case the Automatic Microplate Washer must never beinstalled in dusty places or with caustic fumes.DROP Automatic Microplate Washer dimensions are 40 (width) x 17 (height) x 37 (depth)cm. However it is important to allow sufficient space around the instrument for facilitatingmaintenance operations.

    Leave at least 10 cm on the back, it allows all the electric connections, the right position forthe tubes (straight) and a good heat dissipation.

    2.4 CORRECT INSTALLATION OF THE INSTRUMENT

    The main action to be taken for installing the instrument are: Choice of a person in charge of following all the installation procedures and contacting

    the Manufacturer for any clarifications. Choice of the environment where installation is to take place. Definition of the best position in the installation site. Purchase of additional accessories (supports etc.) apart from those supplied with the

    instrument .

    Although the Automatic Microplate Washer has been designed with components which areable to operate in unfavorable conditions, a thorough check of the surroundings is usefulfor achieving the most reliable performance.

    High temperatures increases component-aging speed, producing temporary and/oreven permanent transformations in the same.

    An extremely dusty environment may cause an abrasive action on the componentsand therefore reduce their life.

    Vibrations may cause deterioration of the mechanical parts of the instrument. High frequency and high intensity pulses generated in the electronic system or induced

    by the surroundings may cause errors in the system. Do not place the instrument near any heat sources like radiators, hot air pipes or in

    direct sunlight. Try to avoid rooms where sudden temperature changes occur. Always allow adequate ventilation to avoid internal over heating. Install the instrument on a clean, flameproof and flat surface that has at least the same

    dimension of the instrument The instrument must be positioned on the correct feet on the surface.

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    2.5 AC POWER CONNECTION AND GROUNDING

    The room where the instrument will be installed must have a power supply outlet with thefollowing characteristics:

    Distribution : 1 phase, neutral and ground

    Power Supply : 230 VAC

    50 Hz

    Power Consumption : 100 VA

    A cable with three wires and a plug with 3 contacts is supplied for each instrument forconnection to the power supply and grounding. Never connect the instrument to anungrounded outlet.Grounding that does not comply with standards may be a hazard for the users and causemalfunctioning in the instrument.

    2.6 ENVIRONMENTAL CHARACTERISTICS OF THE INSTALLATION ROOM

    Temperature : 10 40 C

    Relative humidity : 80%

    Altitude : < 2.000 m

    Atmosphere pressure : 860hPa-1060hPa.(84.86kPa-104.6kPa)

    This is an instrument of Class A. In a residential district this instrument can makeinterference. In this case it can be demanded the user to take suitable measures.

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    2.7 INSTALLATION

    Model DROP Automatic Microplate Washer consists of main machine, washing solution

    bottle, buffer bottle and waste solution bottle.

    Figure 2.1 - Sketch map

    According to the color on tubing and the lid of Clean Solution Bottle, connect the

    bottle and machine using silica hose.

    After make sure the power supply is correct as above, plug in the power supply cable

    and turn on the power switch

    After self-test program, the following letters of initialization program will appear on the

    LCD.

    Now, Model DROP Automatic Microplate Washer is in a normal state. (i.e. a standbystatus)

    Fill the Clean Solution Bottle with approximately 200mL of distilled water

    Press the PRIME key.

    Check the filling of the solution inlet tubes and the drainage flow current. Wait until all

    the operations have been completed.

    DROP

    Rear Panel of Washer

    Timing valve A B

    Bufferbottle

    Waste

    solutionbottle

    Washing

    solutionBottle

    Electromagnetismvalve

    Washingtip

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    Insert a microplate and push Wash/Stop Key. The instrument will perform a completemicroplate washing procedure as previously programmed

    Empty the distilled water out of the Clean Solution Bottle and Waste Solution

    Bottle bottles and remove the microplate in order to leave the instrument ready fornormal operations.

    2.8 TESTS TO BE PERFORMED WITH THE USER

    During the tests to be made with the user the technician should: Illustrate the features of the instrument, with particular emphasis on the types of

    analysis possible. Explain the operation of each of the controls.

    Illustrate the various operating procedures. Have the user repeat these operations untilhe acquires the necessary skills.

    Illustrate the maintenance operations. Ascertain that the user understands the characteristics of the instrument and has the

    necessary skills to use it correctly.

    2.9 DISINSTALLATION

    Use protective gloves and a mask to carry out all the operations.

    Empty the working solution out of the three Clean Solution Bottle, Waste SolutionBottle and Buffer Bottle bottles.

    Fill the two Clean Solution Bottle and Buffer Bottle bottles with approximately200mL of 10% hypochlorite solution.

    Close the caps on all the bottles.

    Press the PRIME button and wait for all the operations to be completed. Empty the10% hypochlorite solution out of the bottles, wash with distilled water and fill

    the Clean Solution Bottle with approximately 200mL of distilled water.

    Close the caps on all the bottles.

    Wait about 10 minutes and then press the PRIME key again. Wait for all theoperations to be completed.

    Empty the solutions out of the bottles.

    Disconnect the tubes of the bottles.

    The Waste Solution Bottle bottle, together with its cap and relative tubing must all bedisposed of in containers specifically for potentially infected biological waste.

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    3 USE

    3.1 PRINCIPAL BUTTONS

    1 + plus key

    2 minus key

    3 Right cursor key

    4 Left cursor key5 STOP/WASH begin to wash or stop6 PRIME Perform priming procedure

    3.2 FUNCTIONS SELECTION

    Instrument has functions that allow programming of work cycles. Enabled functions areillustrated below:

    Set the program of washing microplate

    Set the row number of microplate to be washed

    Set the times of washing microplate

    Set the immersion time of washing microplate

    Set the volume of injection solution for washing microplate

    Align the position of washing tip

    Set the mode of shaking microplate

    Set the mode of washing microplate Adjust the level of suction solution

    Adjust the level of injection solution

    Adjust the middle position of two-point washing microplate

    Set the special treatment for the last washing microplate.

    Warning of solution level (add this function according to needs)

    Select to equip three channels simultaneously. (Add this function according to needs)

    Adjust the velocity of injection solution or suction solution

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    3.2.1 SELECTION OF WASHING MICROPLATE PROGRAM

    Washing micropate program consists of the row number of washing microplate, the times

    of washing microplate and immersion time of washing microplate

    Adjust the code of washing microplate program so that LCD will display the corresponding

    three parameters including the row number of washing microplate, the times of washing

    microplate and immersion time of washing microplate. Press the wash button to save the

    edited parameters.

    Press or key to move cursor to the figure that program indicates.

    Press + or to change the code of program. (Adjustable range is 0~99).

    Program Row number Washing times Immersion time Solution volume Alignment

    3.2.2 SET THE ROW NUMBER OF WASHING MICROPLATE

    Press or to move cursor to the figure that row number indicates.

    Press + or to adjust the row number of washing microplate (adjustable range is1~12).

    Program Row number Washing times Immersion time Solution volume Alignment

    3.2.3 SET THE TIMES OF WASHING MICROPLATE

    Press or to move cursor to the figure that washing times indicates

    Press + or to adjust the times of washing microplate. (Adjustable range is 0~20)

    letters A means 10 times

    letters B means 11 times

    letters C means 12 times

    letters D means 13 times

    letters E means 14 times

    01 12 5 05 65 2

    01 12 5 05 65 2

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    letters F means 15 times

    letters Gmeans 16 times

    letters H means 17 times

    letters I means 18 times

    letters J means 19 times

    letters K means 20 times

    Program Row number Washing times Immersion time Solution volume Alignment

    3.2.4 SET IMMERSION TIME OF WASHING MICROPLATE

    Press or to move cursor to the figure that time indicates

    Press + o to adjust the immersion time of washing microplate. (Adjustable range is0~FK. (0s~1200s), step length is 5s)

    Program Row number Washing times Immersion time Solution volume Alignment

    3.2.5 SET THE INJECTION SOLUTION VOLUME OF WASHING

    MICROPLATE

    Press or to move cursor to the figure that solution volume indicates.

    Press + o to adjust the injection volume. (Adjustable range is 0~99).

    Program Row number Washing times Immersion time Solution volume Alignment

    01 12 5 05 65 2

    01 12 5

    05 65 2

    01 12 5 05 65 2

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    SBSBSBSB:0 MD: 2

    3.2.6 ALIGN THE POSITION OF WASHING TIP

    Check out whether two rows of needles of washing tip are aligned with the first row of

    micro-well of the microplate and whether the washing tip will be moved to right side by

    1mm after pressing + or key each time till the proper position is achieved (that is, thetwo rows of needles can insert right into the micro-well of microplate when the washing tip

    is lowered down)

    Press or to move cursor to the figure that alignment indicates.

    Press + o to adjust the position of washing tip. (Adjustable range is 0~9)

    Note:Aligning the position of washing tip only affects washing mode 1 and 3 instead of

    mode 2.

    Program Row number Washing times Immersion time Solution volume Alignment

    3.2.7 MODE OF SHAKING MICROPLATE

    Press or to move cursor to the figure that SB indicates

    Press + o to change the mode of shaking microplate. (Adjustable range is 0~5).

    Mode 0 means no shaking microplate

    Mode 1 means general shaking following automatic washing microplate.

    Mode 2 means the frequency of shaking is bigger that mode 1 and automatic washing

    microplate after shaking

    Mode 3 means light shaking and no washing microplate

    Mode 4 means stronger shaking and no washing microplate

    Mode 5 means strongest shaking and automatic washing microplate after shaking

    01 12 5 05 65 2

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    SBSBSBSB: 0 MD:2

    PLPLPLPL: 019 IL: 014

    PLPLPLPL: 019 IL:014

    3.2.8 MODE OF WASHING MICROPLATE

    Press or to move cursor to the figure that MD indicates

    Press + o to change the mode of washing microplate. (Adjustable range is 1~5).

    Mode 1 means general washing microplate

    Mode 2 means two-point suction washing microplat

    Mode 3 means single strip washing microplae

    Mode 4 means two-point suction and bottle-rinsing washing microplate

    Mode 5 means general and bottle-rinsing washing microplate

    3.2.9 ADJUST THE LEVEL OF SUCTION SOLUTION

    Press or to move cursor to the figure that PL indicates

    Press + o to adjust the level of suction solution. (Adjustable range is 3~24).

    Note: Whether the adjustment of this parameter is appropriate or not will directly affect the

    residual solution volume after washing..

    3.2.10 ADJUST THE LEVEL OF INJECTION SOLUTION

    Press or to move cursor to the figure that IL indicates.Press + o to adjust the level of injection solution. (Adjustable range is 3~20).

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    XILXILXILXIL:03 FT: 01

    XILXILXILXIL: 03 FT: 01

    3.2.11 ADJUST THE MIDDLE POSITION OF TWO-POINT WASHING

    MICROPLATE (MODE 2 OF WASHING MICROPLATE)

    Press or to move cursor to the figure that XIL indicates

    Press + o to adjust the middle position of two-point washing microplate . (Adjustablerange is 1~5).

    Note: Whether the adjustment of this parameter is appropriate or not will directly affect

    the motion of washing tip in two-point washing microplate. (That is, mode 2 of

    washing microplate) instead of that in mode 1 and 3.

    3.2.12 ADJUST THE SPECIAL TREATMENT FOR THE LAST WASHING

    MICROPLATE

    Press or to move cursor to the figure that FT indicates

    Press + o to adjust the washing effect of last washing microplate. (Adjustable range is1~5).

    Note: Whether the adjustment of this parameter is appropriate or not will directly affect

    the residual solution volume after washing

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    3.2.13 ADJUST THE VELOCITY OF INJECTION SOLUTION OR SUCTION

    SOLUTION

    Adjust the velocity of injection solution or suction solution by the timing valve on the rear

    panel of the washer.

    3.2.14 RINSING HOSE

    Press PRIME button to make washer automatically complete the washing. And LCD will

    display the following figure.

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    4 MAINTENANCE AND TROUBLESHOOTING

    4.1 DAILY MAINTENANCE

    Routine maintenance will keep the instrument in optimum working conditions.

    Cleaning or replacing parts that have come into contact with dangerousbiological materials may cause contamination. Always wear appropriateprotective gloves and face mask during these operations.

    Disconnect power supply before maintenance!

    Solution must be strictly prevented from entering the pump. After each operation,dispose the waste solution in the waste bottle in time. Before each operation, make surethat the waste bottle is clean. During operation, level of waste solution in waste bottlecannot exceed the indicated borderline. User should pay more attention to this ifwashing more than two pieces of microplates every day.

    Before and after operation each time, rinse the hose with distilled water, which is themost effective method to prevent washing tip from jamming due to crystallization ofwashing solution.

    4.1.1 CLEANING THE OUTSIDE OF THE INSTRUMENT

    External Cleaning of the instrument:

    Clean the external surface of the instrument with a brush to remove the dust

    Use a slightly damp rag to eliminate any signs of dirt

    Warning: Do not use air compressors or solvents that may damage the paint

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    4.2 TROUBLESHOOTING

    No. Trouble Possible Causes Remedies

    Power is not turned on; Turn power on.

    Fuse burned out; Replace fuse.

    1 Washer does not work;No display on screen afterwasher is turned on.

    Power cable is notconnected properly.

    Connect power cablewell.

    2 Needles of washing tipcannot insert into micro-well of microplate.

    The firs row is in wrongposition.

    Re-align the position ofwashing tip.

    Suction nozzle of washingtip does not connect withthat in front of the washer;

    Connect pipeline well.

    The lid of waste solutionbottle is not tight;

    Tighten the lid of bottle.

    Air suction nozzle does notconnect with negativepressure air nozzle of mainmachine;

    Connect pipeline well.

    Silica gel hose breaks orhas a dead knot in hoseline;

    Replace silica gel hoseor remove the knot.

    The long needle of washing

    tip is blocked;

    Use steel wire of 1

    or needle to clear theblocked needle hole.

    3 Solution cannot be sucked.

    Negative pressure airnozzle of washer fails tosuck air.

    Contract manufacturer.

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    No. Trouble Possible Causes Remedies

    Solution outlet nozzle ofwashing tip does notconnect with that in front ofwasher;

    Connect pipeline well.

    Lid of washing solutionbottle is not tight enough;

    Tighten the lid.

    Air suction nozzle ofwashing solution bottledoes not connect with

    positive pressure air nozzleof washer;

    Connect pipeline well.

    Silica gel hose breaks orhas a dead knot in hoseline;

    Replace silica gel hoseor remove the knot.

    The short needle ofwashing head is blocked;

    Use needle of 0.5 toclear the blocked fineneedle hole.

    Washing solution bottle hasno solution;

    Add solution

    No air comes out of positivepressure air nozzle ofwasher;

    Contract withmanufacturer.

    4 Washing solution cannotbe added.

    Electromagnetism valve isclose.

    Check the line