5 mab peach breeding arus pere

• 1. MARKERS FOR PEACH BREEDINGYOUR LOGO

2. 1. PARENT CHARACTERIZATION AND SELECTION Relationships between parents Cross planning Genetic distances Heterozygosity Breeders rights enforcement2. SEEDLING MARKER-ASSISTED SELECTION YOUR LOGO 3. MARKER (SSR) FINGERPRINTING FOR BREEDERS RIGHTS PROTECTION251261280280246 280251246261261189 196139139189 196189 196196189 196YOUR LOGO135148148148139135 139148 4. PEDIGREE VALIDATION 167P1P2141178183147189178 183141H-A178H-B189125141YOUR LOGO147147129131125 131125 131 5. SELECTING PARENTS FOR A CROSS: GENETIC DISTANCE The DENDROGRAM Clusters group genetically similar parents Do my pedigree data fit?Do I need more variability? Do I want to have a fixed background where only a few traits segregate? 6. SELECTING PARENTS FOR A CROSS: HETEROZYGOSITY % Heterozygosity 0102030405060More heterozygosity in the parents More segregation in their progeny 7. MARKER-ASSISTED SELECTIONGM1M2APPROPRIATE USE OF MARKERS REQUIRES DETAILED KNOWLEDGE OF THE PARENTS OF EACH BREEDING PROGRAM 0-5 cMLINKED MARKERS: Require validation in a progeny (phase knowledge) DIAGNOSTIC MARKERS: Presence of certain alleles predict corresponding phenotypes YOUR LOGO 8. a tDhncia ellHRINEgPEACH MAJOR GENES used for Marker-Assisted Selection (MAS) Male sterilityAcidityPeach/Nectarine Pubescencia de la pellMELTINGNECTARINEColor de la polpaConsistencia de la polpaPEACHFlesh colorNON-MELTINGWHITEYELLOWG/gY/y Forma del fruitFlesh texture Consistencia de la polpaM/mFruit shapePubescencia de la pellConsistencia de la polpaForma del fruitPEACHMELTINGROUNDNECTARINEColor de la polpaNON-MELTINGColor de polpaMD MELTINGROUNDWHITEWHITEMaturity date NON-MELTING NON-MELTINGM/m F/fFLATSh/shYELLOWY/yYOUR LOGOG/gM/mFLATSh/shYELLOWY/y 9. Marker Assisted Breeding (MAB) workflow - ARE THERE MARKERS AVAILABLE FOR THE TRAIT? YESNO(Ex: acidity, flat shape)(Ex: pistilar closing)Use the marker or saturate the region in a segregating population to find a better markerFind or develop a segregating population and construct a genetic map and look for the position of MAJOR GENES or QTLsPrunus reference map (Dirlewanger et al. 2004, PNAs 101(26):9891-9896)validate markers in germplasm collectionPeach genome (www.rosaceae.org)MASYOUR LOGO 10. INTRODUCTION of MAB in the IRTA BREEDING PROGRAM 1st STEP: DEMONSTRATION: plants in the field phenotype known or that could be determined # plants analyzed by fruit shape# plants analyzed by acidityYears1,3111,3492010-2012Less than 5 % of errors in phenotype predictions with either marker 2nd STEP: SEEDLING SELECTION: selection in the greenhouse # plants analyzed by fruit shape# plants analyzed by acidityYears1,2451,5022012-201366% of the seedlings of the program in 2013 were selected with markers: All except small progenies and fixed progenies YOUR LOGO 11. THANK YOUYOUR LOGO