440-LC Fraction Collector P. van Poppel – Field Support Specialist October 2009.

440-LC Fraction Collector

P. van Poppel – Field Support Specialist October 2009

Overview

•Introduction to 440-LC

• Options

• Usage

•Operation and Control

•Installation

•Problems

440-LC Fraction Collector

1. Theta axis

2. Z axis slide

3. Retaining ring

4. Probe mounting block

5. Ridged mount nut on the probe assembly

6. Ferrule holding the metal probe

7. Probe

8. Rack

9. Rack location mat

440-LC Fraction Collector Options

•High-flow kit including high-flow PEEK™ probe, diverter valve and funnel rack •High-flow probe •Funnel rack •USB to RS232 converter •Various racks:– 90 position x 13 mm OD (7 mL) rack – 60 position x 16 mm OD (13 mL) rack – 40 position x 20 mm OD (25 mL) rack – 24 position x 25 mm OD (38 mL) rack – 21 position x 30 mm OD (50 mL) rack – Rack overlay for 40 position rack, 18 mm OD – Rack overlay for 60 position rack, 16 mm OD

Specifications

•Large fraction capacity

•Wide flow rate range up to 800 mL/min

•Compatible with range of HPLC detectors

•Simple, intuitive software control

•Compact footprint (600 x 400mm)

Usage

Control

Installation

•Connect up power

•Connect serial cable between PC and F/C

•Calibrate tray

•Save coordinates

•Install software

•Determine delay time

Problems

•Fractions not correlating to the tick marks on the Galaxie software

•Liquid is not delivered to the tubes

•Probe hits the top of the tubes or is not low enough

•Error status LEDs are showing an error

•The fraction collector shows offline in Status Overview

•The arm is not moving correctly in the X, theta or Z axes

•Fraction collector is not initializing