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“Improving Cowpea Productivity for Marginal Environments in SSA”
UC Riverside and IITA (Jeff Ehlers, Phil Roberts, Tim Close, Ousmane Boukar) N. Cisse - ISRA, Senegal I. Drabo - INERA, Burkina Faso R. Chiulele - Eduardo Mondlane University, Mozambique
200mm
800mm
Partners located across semi-arid “cowpea zone”: IITA – Kano, Nigeria
TL- 2 IITA/NARS MAS & MABC; Test, release varieties
Data Management Genotyping, MARS Tools QTL Analysis
TL-1 MAGIC population
Training
Agarose gel markers
cDNA, EST sequencing
High-throughput SNP genotyping platform
8 elite x elite , 2 cycles MARS; 8 MABC sets of lines
24 advanced lines w/superior performance under drought; 8 MABC lines
Test - MARS efficiency, practicality
3 PhDs trained
7 NARS breeders trained
Transfer. Outcome
Activity 1: Develop MAGIC population
SNP genotypes for the eight prospec3ve parents for linkage group 1 are shown below as an example.
Activity 2: Develop genomic resources in support of marker-assisted breeding
• Customized sets of markers for MARS ; genotype data production in support of MARS breeding
• Genotyping data analysis to optimize MARS (breeding values, selection indices)
• Genetic analysis for QTL discovery in MARS populations
Activity 3: Employ MARS and MABC to develop improved breeding lines
• 2 populations per partner, 300 lines/population • Conduct MARS cycles 1 and 2 in elite x elite crosses • Selection and performance testing of advanced lines
Genotype 5/family, recombine selected F4, also advance selects to F5-tests Genotype, advance F5 to F6
Performance evaluation to determine progress
Genotyping Needs/popln 92 10x5 300 300
30x5
10x5
10x2 400
10x5
10x5
10x5
10x5 100 300
No. of Populations 2 2 6 2 6 2 2 6 6 2 6 6 6 6
Total=10,727 samples to be genotyped 184 100
1800 600 900 100 40
2400 300 100 300 300 600
1800
**Yellow and Blue filled areas are for populations initiated for TL-1 Phase 2 and under the Top-Off funding in 2009, respectively; Red indicates work already completed during Phase 1 as part of Phase 2; Light blue and light yellow indicates new cycle being initiated for TL-1 Phase 1 ‘Top-Off’ and Phase 2 populations, respectively. Top-Off populations (SuVita2/Mouride and IT93K-503-1/IT84S-2246) currently consist of 92 F4 families each, with phenotyping being conducted at UCR, Senegal and Burkina Faso. The 6 Phase 2 MARS founder populations (Table 2) will consist of 300 F3:4 families.
Cross Partner #
polymorphic markers
Known resistance/tolerance traits present in population