39 Matthew Blair Tli Objective3 Phase Ii Work Plan

TL1 proposal: common bean

TL2 annual Meeting, Bamako Mali – Nov. 2009

(Objective 3 – Tropical Legumes I)

Objective 3 Common bean – Major Outcome flows to TL-1 Phase II

Germplasm Screening

Genomic Resources

Marker Associations

Population development

SSR platform (1500) from EST, BAC, etc., and

genotyping sets 1st gen. SNP platform (768)

Germplasm genotyped

Germplasm phenotyped (604) drought tolerance

Sources of drought tolerance

Diversity Sets: Ethiopia, Kenya,

ECABREN / SABRN and

Reference Coll.

Deep rooting sources

Mapped markers / Physical map

QTL for deep rooting and for carbohydrate

translocation

Marker-assisted backcrossing to develop improved

local varieties

MAS crosses (CBB, BCMV, Bruchid)

Adv Backross populations created (5)

Early generation populations and advanced lines

Adv. Backcross and MARS breeding populations for

Andean drought tolerance

2007

2010

2014

Phase I Phase II

Activity

Tools

Operation

Outcome

SNP confirmation

For Intra-genepool populations

QTL populations phenotyped (deep

rooting and carbohyd. translocation)

Intra-genepool Elite x Elite Crosses (40)

Inter-genepool lines (DAB and CBIB lines)

Advanced lines and segregating

populations

Act. 1: Evaluate rooting depth of parental sources

•  Extending work on reference and regional collections (G. Makunde, Ph.D. thesis)

Act. 2: Validate SNPs for inter- and intra-gene pool crosses

•  For 50 donor and recipient genotypes

•  For introgression to Andeans

•  For use in MARS

Andeans

Mesoamericans

Act. 3: Deploy insect and disease resistance genes

•  Confirm MAS in advanced lines –  BCMNV –  Storage insects –  Bacterial Blight

•  RGA for Bacterial blight

Act. 4: Deploy drought tolerance traits from

inter-gene pool crosses in Andean

gene pool

•  Characterize traits in 40 parentals

•  Develop & test visual scales

•  QTL for Pod Harvest Index & deep rooting

•  Test 60 Andeans for heat tolerance

ooooo

Pod Partitioning

Index

Pod Harvest Index

Act. 5: Pyramid drought tolerance traits through MARS and Advanced Backcross

in Andean gene pool •  Genotype 2 AB

populations

•  Genotype & phenotype 4 RIL populations for MARS

•  3 more populations for MARS

•  Pyramiding from AB and RILs

P1 P2

F1

F1-BC1

F1-BC2

X

Advanced Backcross Lines

AdvancedBackcrossScheme

QTL identification

Phenotype

Genotype Genetic Map

Mesoamerican Drought Tolerance

Sources SER16 SER48

Microsatellites and SNPs Andean

Red Mottled Cultivars

CAL96 (Uga) CAL143 (Mwi)

2 AB-QTL Populations

selected

Line distribution

F2: Drought Single Plant Selection Lowland site

F3: Plant select. Low P + MAS for R gene Highland site

F3.4: Families Lowland site

F1 to F2

F3.5: Drought Multi-location trials option

Phenotyping for QTL analysis Double & Multiple

crosses

DNA extraction

Genotyping

QTL analysis

F1: Simple cross Drought tol. X And. commercial

Lines Derived and Recombination Cycles

MarkerAssistedRecurrentSelectionScheme

Cycle 1 starts

Act. 6: Training of students in drought tolerance and MAS

•  Training students in MARS •  Short course in CIAT

Act. 7: Data storage and management

Proposed Act. 8: Testing the out-sourcing of marker analysis with partners

•  Samples currently cost $8 in Africa (paid by special project using unique method)

•  Outsourcing would cost $2-3

•  Accuracy of markers would be verified – Bacterial blight – Storage insects