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D–3242

DISTANCE EDUCATION

M.Sc.(Microbiology) DEGREE EXAMINATION, DECEMBER 2019.

First Semester

GENERAL MICROBIOLOGY

(CBCS- 2018-19 Academic year onwards)

Time : Three hours Maximum : 75 marks

SECTION A — (10 2 = 20 marks)

Answer ALL questions.

Define/comment on:

1. Protista

2. Gram positive bacteria

3. Natural media

4. Lyophilization

5. Stationary phase

6. Capsule

7. Protozoa

8. Mitochondria

9. Viroids

10. DNA Viruses

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SECTION B — (5 5 = 25 marks)

Answer ALL questions

11. (a) Write the short notes on introduction to microbiology.

Or

(b) Write short notes on Whittaker's five kingdom concept.

12. (a) Write short notes on anaerobic culture.

Or

(b) Write short notes on nutritional types of bacteria.

13. (a) Write the general characteristics of BGA.

Or

(b) Give a brief account on classification of protozoa.

14. (a) Give a brief note on economic importance of algae.

Or

(b) Write short notes on bacterial endospore.

15. (a) Write the distinctive properties of viruses.

Or

(b) Give a brief account on life cycle of virus.

SECTION C — (3 10 = 30 marks)

Answer any THREE questions.

16. Explain the classification of fungi based on Alexopoulus system.

17. Explain the principle and applications of confocal microscopy.

18. Explain in detail about the flagella and motility.

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19. Explain the structure of eukaryotic cell with suitable illustration.

20. Give an account on types of virus envelope and their compositions.

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Ws15

D– 3243

DISTANCE EDUCATION

M.Sc. DEGREE EXAMINATION, DECEMBER 2019.

First Semester

Microbiology

MICROBIAL BIOCHEMISTRY

(CBCS – 2018-2019 Academic year onwards)



Answer ALL the questions

1. Agar-Agar

2. Peptidoglycan

3. Peptides

4. β sheet

5. Phospholipids

6. Nucleosides

7. Apoenzyme

8. Abzyme

9. Aflatoxin

10. Tocopherol

Sub. Code 36412

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Answer ALL the questions

11. (a) Write short notes on Structure of disaccharides.

Or

(b) Give an account on structure of cellulose.

12. (a) Briefly explain the biological importance of amino acids.

Or

(b) Add short note on primary structure of protein.

13. (a) Describe the synthesis of cholesterol in E.coli.

Or

(b) Give an account on degradation of pyrimidines.

14. (a) Write brief note on chemical nature of enzymes.

Or

(b) Illustrate the allosteric inhibition of enzymes.

15. (a) Write a brief account on bacteriochlorophyll.

Or

(b) Give brief note on functions of fat soluble vitamins.


Answer any THREE questions

16. Discuss in detail about types of polysaccharides with suitable example.

17. Write elaborate note on classification of amino acids.

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18. Explain in detail about β – oxidation of fatty acids.

19. Write an essay on Lock and key model and induced fit theory of enzymes.

20. Describe the biological functions of Co -factors and Co-enzymes.

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Ws9

D–3244

DISTANCE EDUCATION


First Semester

Microbiology

MICROBIAL PHYSIOLOGY

(CBCS 2018 – 2019 Academic Year Onwards)


PART A — (10 2 = 20 marks)


Define/Comment on :

1. Autotrophy.

2. Chemoheterotrophism.

3. Chlorophyll.

4. Phosphorylation.

5. Osmosis.

6. Symbiosis.

7. Anaerobic respiration.

8. TCA.

9. Quorum Sensing.

10. Energy bond.

Sub. Code 36413

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PART B — (5 5 = 25 marks)


11. (a) Write short notes on nutritional types.

Or

(b) Write a brief note on factors affecting microbial growth.

12. (a) Write short notes on carotenoids.

Or

(b) Write short notes on aerobic to anaerobic transitions.

13. (a) Write short notes on mechanism of oxidative phosphorylation.

Or

(b) Describe in brief account on sulphate respiration.

14. (a) Write short notes on respiratory electron transport in mitochondria.

Or

(b) Write short notes on Amphibolic reactions.

15. (a) Write a short note on entropy and enthalpy.

Or

(b) Give a brief account on group translocation.

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PART C — (3 10 = 30 marks)


16. Explain the Physiology and economic importance of methylotrophs.

17. Explain about the microbial growth curve.

18. Explain about the general types of microbial photosynthesis.

19. Give an account on Nitrogen cycle.

20. Explain in detail about bioenergetics.

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WS19

D–3245

DISTANCE EDUCATION


Second Semester

Microbiology

MICROBIAL GENETICS





Define/Comment on:

1. Mutagenesis

2. Thymine dimer

3. Base analog

4. Recombination

5. F factor

6. Trp operon

7. Colicinogeneic

8. Intergerons

9. Transposons

10. Phage Mu

Sub. Code 36421

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11. (a) Write a short notes on mutation rate.

Or

(b) Give a brief account on SOS repair mechanism.

12. (a) Explain in brief about Hfr conjugation.

Or

(b) Elaborate the transformation mechanism in Bacillus subtilis with streptococcus pneumonia.

13. (a) Write short notes on generalized transduction.

Or

(b) Give a brief notes on negative gene regulation.

14. (a) Give a brief notes on host range plasmids.

Or

(b) Explain briefly about the replications of plasmids.

15. (a) Write short notes on reterotranposons.

Or

(b) Give a brief account on bacteriophage lS911.



16. Write in detail about the mutations and its types.

17. Explain in detail about the deamination of base pairs, alkylation and UV induced DNA damages.

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18. Write an account on regulation of gene expression in Arabinose operon system.

19. Give a detail account on genetic organization and transposition of Tn5 and Tn3.

20. Discuss in details about the bacterial epigenetics.

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D–3246

DISTANCE EDUCATION


Second Semester

Microbiology

MOLECULAR BIOLOGY AND rDNA TECHNOLOGY




Answer ALL the questions.

1. Z - form of DNA

2. Topoisomerase

3. Okazaki fragments

4. Transcription factors

5. Cosmids

6. SV-40 vectors

7. cDNA library

8. Nitrocellulose membrane

9. RAPD

10. Microarray

Sub. Code 36422

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Answer All the questions

11. (a) Write short on Denaturation and renaturation of

nucleic acids.

Or

(b) Write in brief about DNA polymerase enzyme.

12. (a) Give short notes on tRNA.

Or

(b) Describe the post transcriptional modification in

prokaryotes.

13. (a) Explain about structure of phagemids.

Or

(b) Give an account on YAC vectors.

14. (a) Give brief note on shot gun cloning.

Or

(b) Write about the significance of western blotting in

molecular diagnosis.

15. (a) Write a brief account on Maxam Gilbert's method of

sequencing.

Or

(b) Write briefly about mechanism of Gene silencing.

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16. Write the molecular basis of DNA as genetic material.

17. Explain the salient features of pBR322.

18. Write elaborate notes on commercial production of antibiotics.

19. Discuss about the primer designing and optimization.

20. Give detailed account on microinjection and electroporation.

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D–3247

DISTANCE EDUCATION


Second Semester

Microbiology

FOOD AND DAIRY MICROBIOLOGY




Answer ALL questions:

Define/Comment on:

1. Mould

2. pH

3. Redox Potential

4. Canned food

5. Relative humidity

6. Intoxication

7. Preservative

8. Fermentation

9. Cellulase

10. HACCP

Sub. Code 36423

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11. (a) Write short notes on food related microorganisms.

Or

(b) Brief notes on nutritional content of the foods.

12. (a) Write short notes on food poisoning.

Or

(b) Elaborate the quality checking of milk.

13. (a) Give brief account on SCP.

Or

(b) Write critical notes on amylase.

14. (a) Write a short note on industrial production of protease.

Or

(b) Explain physical preservation methods.

15. (a) Discuss about the food sanitations?

Or

(b) Write a short note on food laws.



16. Write in detail about the Intrinsic and extrinsic factor for food.

17. Give a detailed account on food preservation methods and its types.

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18. Give an account on microbial fermentation food products.

19. Elaborate the preventive measures of food borne diseases.

20. Write a detailed account on AGMARK and BIS.

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wk14

D–3248

DISTANCE EDUCATION

M.Sc. (Mircobiology) DEGREE EXAMINATION, DECEMBER 2019.

Third Semester

IMMUNOLOGY



PART A — (10 2 = 20 marks)


Define/Comment on:

1. Haematopoiesis

2. Innate immunity

3. Complement

4. Adjuvants

5. Precipitation

6. Oncogenes

7. DNA vaccine

8. MHC

Sub. Code 36431

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9. Monoclonal antibody

10. Hypersensitivity

PART B — (5 × 5 = 25 marks)


11. (a) Write a short notes on concepts of immunology.

Or

(b) Explain briefly on cell mediated immune response.

12. (a) Write short notes on humoral mediated immune response.

Or

(b) Explain about the maturation of B – cell.

13. (a) Write a short notes on T– cell receptor.

Or

(b) Explain briefly about Haemokines.

14. (a) Write a short notes of antibody diversity.

Or

(b) Explain briefly about Haptens.

15. (a) Explain briefly about MHC.

Or

(b) What are the types of vaccines? Briefly explain.

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PART C — (3 10 = 30 marks)

Answer any THREE Questions.

16. Give an account on structure and functions of immune system.

17. Explain in detail about Immunoglobulin and their types.

18. Describe in detailed about mechanisms of Antigen processing.

19. What is Stem cells? Explain and its clinical applications.

20. Describe about Auto immune disorders.

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D–3249

DISTANCE EDUCATION


Third Semester

Microbiology

MEDICAL MICROBIOLOGY

(CBCS – 2018-19 Academic Year Onwards)


PART A — (10 2 = 20 marks)


Define/Comment on:

1. Bio safety

2. CSF

3. Throat swab

4. Microbial infection

5. Pharyngitis

6. Antibiotic

7. SARS

8. Poliomyelitis

9. Amoebiasis

10. Ebola.

Sub. Code 36432

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PART B — (5 5 = 25 marks)

Answer ALL questions, choosing either (a) or (b).

11. (a) Write short notes on microbiological examination of sputum.

Or

(b) Explain briefly about nosocomical infection.

12. (a) Explain about pathogenesis of Gonorrhea.

Or

(b) Write short notes on Diptheira.

13. (a) What are the control measures of pertusis?

Or

(b) Explain briefly about Salmonellosis.

14. (a) Write short notes on Rabies.

Or

(b) Explain briefly about Swine flu.

15. (a) Explain briefly about H1N1 virus.

Or

(b) Write short notes on the mode of action of metraindazole.

PART C — (3 10 = 30 marks)


16. Give an account on microbial examination of urine.

17. Describe about characteristic laboratory diagnosis and control measures of Yersiniosis.

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18. Explain detailed about tuberculosis.

19. Describe detailed about Hepatitis.

20. Explain detailed about mode of action of penicillin.

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WS19

D–3250

DISTANCE EDUCATION


Third Semester

Microbiology

ENVIRONMENTAL AND AGRICULTURAL MICROBIOLOGY





1. Food chains

2. Eutrophication

3. Saccharification

4. Oxidation pond

5. Xenobiotics

6. VAM

7. Phosphorus mobilization

8. Phyllosphere

9. Flavonoids in plants

10. Bunchy top of banana

Sub. Code 36433

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11. (a) Describe the composition and structure of the environment.

Or

(b) Give a brief account on food webs.

12. (a) Write short note on vermi composting.

Or

(b) Briefly explain about trickling filter.

13. (a) Write about the activated sludge digestion process.

Or

(b) Add short notes on biomagnification.

14. (a) Briefly explain the properties of soil.

Or

(b) Describe the structure of soil.

15. (a) Write an account on rhizospheric microbes.

Or

(b) Add a brief note on bacterial blight of paddy.



16. Explain in detail about ecological pyramids.

17. Write elaborate note on solid waste management.

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18. Explain the symbiotic association of bacteria in legume plant.

19. Describe in detail about the carbon cycle.

20. Discuss the various methods of plant disease management.

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