2998 PDA DETECTOR - LabWrench

T he perfecT maTch for any Lc sysT em

Waters® 2998 Photodiode Array (PDA) detector is designed to

complement any of Waters analytical HPLC, preparative HPLC, or

LC/MS system solutions. Its uniquely integrated software and optics

innovations deliver no-compromise chromatographic and spectral

sensitivity, and a noise specification as low as 10 µAU. It is the

ideal detector for any lab application from compound identification

to method development. For routine analyses, the 2998 PDA detec-

tor is reliable, easy-to-use, and has enhanced software control to

provide flexibility for simultaneous 2D and 3D operation with either

Empower™ or MassLynx™ software.

The 2998 pDa detector provides:n Unprecedented trace impurity detection and quantification.

n Definitive compound identification and co-elution detection.

n Superior linear range with constant optical bandpass.

n Uncomplicated method development.

2998 P DA D E T EC T O R

Ultimate chromatographic sensitivity in photodiode array detection

2998 Photodiode Array detector.

2998 PDA detector with its choice of flow cells can easily be integrated into Waters HPLC and LC/MS system solutions, such as the Alliance® HPLC system or the AutoPurification™ system.

Minutes

AU

0.00 2.50 5.00 7.50 10.00 12.50 15.00

0.0045

0.0030

0.0015

0.0000

Om

epra

zole

- 7

.491

Peak

2 -

1.9

11

Peak

1 -

1.7

13

Peak

3 -

2.5

91

Peak

4 -

2.8

48

Peak

5 -

4.4

38

Peak

6 -

4.7

95

Peak

7 -

6.0

48

2998 PDA detector enables exceptional signal-to-noise (S/N) ratios to quantify low- level impurities, and determine trace components.

peak area % signal-to-noise

Peak 1 0.003 4

Peak 2 0.031 35

Peak 3 0.005 5

Peak 4 0.022 22

Peak 5 0.033 25

Peak 6 0.008 4

Peak 7 0.006 3

Omeprazole 99.89 44485

GroUnD-BreaKInG T echnoLoGy TaK es pDa DeT ecT Ion To T he neXT LeV eL

Instrument console —2998 PDA detector interactive display.

spectral exposure optimizationn Maximizes the signal-to-noise across the entire

wavelength range.

n Improves noise performance in lower energy regions of

the spectrum while maintaining optimal performance

across the range.

console software

The 2998 PDA detector is equipped with a customizable instrument

console compatible with both Empower and MassLynx software,

enabling users to easily stay in control. Instrument setup, status

monitoring, and diagnostics are easily accessible through the

software’s intuitive, easy-to-learn interface.

The console interface:

n Uses a simple navigational approach, for easy system

implementation and instrument usability.

n Allows for quick and easy access to critical instrument

parameters, enabling the detector to be easily controlled,

monitored, and diagnosed.

Conventional cell

window

UV light

lens

TaperSlit™ flow cell

window

window

patented Taperslit flow celln Maximizes energy throughput.

n Eliminates refractive index effects.

n Maintains good peak shape, ensuring the highest sensitivity

while maintaining optimal spectral performance.

Lamp optimizationn Designed to automatically maximize signal-to-noise in

both the visible and UV spectra with the use of a single

Deuterium lamp.

n Extends the useful life of the lamp for consistent results

over time.

Thermal wander managementn Provides maximum baseline stability by managing the

effects of ambient temperature and humidity changes.

n Minimizes vulnerability to hostile environmental conditions.

n Stabilizes long-term baseline wander for high-sensitivity

analysis.

opT ImIZe yoUr DeT ecT Ion anD resoLUT Ion WIT hoUT comp romIse

Definitive compound identification

While most PDA detectors can distinguish between

compounds possessing comparatively large

spectral differences, the 2998 PDA detector can

differentiate between the spectra of closely-related

compounds.

optical and digital resolution

The 2998 PDA detector operates at a fixed optical resolution of

1.2 nm, providing high-quality spectral resolution. The low detector

noise performance allows you to operate at maximum digital resolution

and not sacrifice linearity.

reliable co-elution detection

The 2998 PDA detector combined with Empower software employs

powerful capabilities for determining spectral homogeneity, yielding

confidence in method specificity.

Spectral analysis algorithms can aid in the assessment of peak purity. When low levels of an impurity were spiked into a sample as shown in this lidocaine example, the spectral analysis software was able to detect the impurity at levels as low as 0.1%.

0.00

0.20

0.40

0.60

0.80

3.00 3.20 3.40 3.60 3.80 4.00

0.1

% I

mp

uri

ty1

.0%

Im

pu

rity

511

M

505

M

No I

mpu

rity

Lidoca

ine

- 3.5

17

AU

Minutes

Lidoca

ine

- 3.5

20

Lidoca

ine

- 3.5

05

Lidoca

ine

- 3.5

11

Deg

rees

Deg

rees

Deg

rees

Minutes

Minutes

MinutesAU

AU

PurityAuto Threshold

PurityAuto Threshold

PurityAuto Threshold

0.66

0.44

0.22

0.00

0.88

0.60

0.40

0.20

0.00

0.80

AU

0.60

0.40

0.20

0.00

0.80

3.40 3.45 3.50 3.55 3.60 3.65

3.404 3.450 3.496 3.542 3.588 3.634

3.404 3.450 3.496 3.542 3.588 3.634

80.00

60.00

40.00

20.00

0.00

80.00

60.00

40.00

20.00

0.00

80.00

60.00

40.00

20.00

0.00

Spectral analysis algorithms can distinguish closely related compounds, as shown in this barbiturates example, providing reliable library matching.

Peak 3 - Butalbarbital

Library Match - Butalbarbital

Minutes

AU

nm

AU

AU

superior linearity

Wide linear dynamic range allows for simultaneous quantification of high-

and low-level components within a single chromatographic separation.

Wide linear dynamic range is demonstrated by the linearity plot of propyl paraben at 257 nm.

0

0.5

1

1.5

2

2.5

3

3.5

4

0 2 4 6 8 10 12 14 16

5.0% Deviation at 2.7 AU

1.7% Deviation at 2.0 AU

Abso

rban

ce

Steps

Waters corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com

fast hpLc with Intelligent speed columns

Supporting data rates up to 80 Hz, the 2998 PDA detector allows

for the independent optimization of data rates and filtering

constants. This enables the accurate and reproducible quantitation

of today’s fast LC separations, which generate peaks only a few

seconds wide, as seen with the Alliance HPLC system and Intelligent

Speed™ columns.

Waters and Alliance are registered trademarks of Waters Corporation. Empower, AutoPurification, MassLynx, TaperSlit, Intelligent Speed, and The Science of What’s Possible are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.

©2007 Waters Corporation. Printed in the U.S.A.June 2007 720002187EN LB-PC

powerful pDa data processing with empower software

Demonstrate peak homogeneity Visualize any differences in spectra by using the Spectrum Index Plot to display apex spectrum and other peak spectra — all corrected for noise and normalized in a color-coded overlay plot.

Quantify peak purity For a more quantitative analysis, the Purity Angle Plot mathematically compares the apex spectrum of the peak to that of every data point across a peak. With adjustments to noise and background solvent absorbance, spectral differences and potential impurities become virtually impossible to miss.

Confirm peak iDentity The Library Match function automatically iden-tifies each peak in a sample by mathematically comparing unknown peaks to the reference spectra stored in the library. Once initiated, the library automatically searches for the closest spectral match and reports findings indicating the degree to which it matches. Numerous libraries can be created, stored, searched, and shared among network users.

DoCument wavelength monitoring ChoiCes Automatically select and store maximum absorbencies for each peak. Documentation of the λ-max will justify wavelength selec-tion to auditors.

ConstruCt a multi-wavelength Chromatogram Select and store maximum absorbencies for each peak to automatically construct a multi-wavelength chromatogram, resulting in a meticulous record of all the compounds detected.

DiagnostiC tool anD Confirm methoD ComplianCe Routinely diagnose fluidic performance and confirm method compliance in LC/MS.

Benzo

cain

e -

0.4

43

Pro

cain

e -

0.5

12

Prilo

cain

e -

0.6

91

Lidoca

ine -

0.8

92

Tetr

aca

ine -

0.9

51

Bupiv

aca

ine -

1.1

79

Buta

cain

e -

1.3

13

0.00 0.15 0.30 0.45 0.60 0.75 0.90 1.05 1.20 1.35 1.50

0.845 0.910 0.975 1.0400.80 0.85 0.90 0.95 1.00 1.05

80 Hz

20 Hz

5 Hz

0.1 sec

0.2 sec

0.4 sec

*

*

* 20Hz; 0.1sec

Minutes

MinutesMinutes

Independent manipulation of data rate and digital filtering results in ideal resolution, sensitivity, and repeatable quantification. In this example showing

seven anesthetics, optimization of these parameters yielded a separation in less than 1.5 minutes with baseline resolution.