Ultimate performance and flexibility Designed to deliver the highest performance available in UV/Vis detection, the 2489 UV/Vis Detector offers: ■ ■ Uniquely designed optics ■ ■ Low detector noise (<5 µAU) ■ ■ High-speed data rates ■ ■ Patented TaperSlit™ flow cell ■ ■ Maximum sensitivity for HPLC and fast LC separations 2489 UV/Vis Detector Achieving high UV/Vis sensitivity shouldn’t be complicated SETTING THE STANDARD IN HPLC DETECTION Whether you are doing routine UV-based applications or pushing the limits of detection with low-level impurity analyses, Waters ® 2489 UV/Visible (UV/Vis) Detector is the best detection choice for performance, reliability, and usability. The 2489 UV/Vis Detector is the most sensitive and versatile dual- wavelength absorbance detector available for HPLC. The design combines a high energy Deuterium source, a precise optical design, and low noise, high speed electronics. These advanced capabilities take UV/Vis detection performance to a new level. Trace impurity analysis The detector’s extended linear range of 2.5 AU enables the identification and quantitation of both major and minor components in the same run – a key benefit for trace impurity or metabolism studies. The ability to identify and quantitate low-level impurities in the same chromatographic run as the analyte of interest is a good measure of the detector’s sensitivity. The 2489 UV/Vis Detector easily integrates trace impurities down to 0.006% and lower. 2489 UV/Visible Detector. Conditions: System: Alliance HPLC with 2489 UV/vis Detector Sample: 1.0 mg/ml irbesartan tablet Column: XSelect™ HSS T3, 4.6 mm x 250 mm, 5 µm Injection volume: 8 µL Flow rate: 1.0 mL/min Detection: 220 nm, 40 Hz, 0.05 s (normal) AU 0.0000 0.0009 0.0018 0.0027 0.00 3.60 7.20 Minutes 10.80 14.40 18.00 Impurity 1 – 6.369 Impurity 2 – 9.287 Impurity 3 – 9.719 Impurity 4 – 107.41 RCA – 12629 Irbesartan Tablet – 14.904 Impurity Area % Signal-to Noise Impurity 1 0.006 53 Impurity 2 0.011 70 Impurity 2 0.034 213 Impurity 2 0.003 18 RCA 0.018 89 Irbesartan Tablet 99.927 422602
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2489 UV/Vis Detector - Waters Corporation · high-speed data rates and independent filter optimization The 2489 UV/Vis Detector offers a wide variety of sampling rates, from 1 to
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Ultimate performance and flexibility
Designed to deliver the
highest performance
available in UV/Vis
detection, the 2489 UV/Vis
Detector offers:
■■ Uniquely designed optics
■■ Low detector noise
(<5 µAU)
■■ High-speed data rates
■■ Patented TaperSlit™
flow cell
■■ Maximum sensitivity
for HPLC and fast
LC separations
2489 UV/Vis DetectorAchieving high UV/Vis sensitivity shouldn’t be complicated
Set t ing t he Standard in hPLC det eCt ion
Whether you are doing routine UV-based applications or pushing
the limits of detection with low-level impurity analyses, Waters®
2489 UV/Visible (UV/Vis) Detector is the best detection choice for
performance, reliability, and usability.
The 2489 UV/Vis Detector is the most sensitive and versatile dual-
wavelength absorbance detector available for HPLC. The design combines
a high energy Deuterium source, a precise optical design, and low noise,
high speed electronics. These advanced capabilities take UV/Vis detection
performance to a new level.
trace impurity analysis
The detector’s extended linear range of 2.5 AU enables the
identification and quantitation of both major and minor components in
the same run – a key benefit for trace impurity or metabolism studies.
The ability to identify and quantitate low-level impurities in the same chromatographic run as the analyte of interest is a good measure of the detector’s sensitivity. The 2489 UV/Vis Detector easily integrates trace impurities down to 0.006% and lower.
2489 UV/Visible Detector.
Conditions:System: Alliance HPLC with 2489 UV/vis DetectorSample: 1.0 mg/ml irbesartan tabletColumn: XSelect™ HSS T3, 4.6 mm x 250 mm, 5 µmInjection volume: 8 µLFlow rate: 1.0 mL/min
Detection: 220 nm, 40 Hz, 0.05 s (normal)
AU
0.0000
0.0009
0.0018
0.0027
0.00 3.60 7.20 Minutes
10.80 14.40 18.00
Impu
rity
1 –
6.36
9
Impu
rity
2 –
9.28
7
Impu
rity
3 –
9.71
9
Impu
rity
4 –
107.
41
RCA
– 1
2629
Irbes
arta
n Ta
blet
– 1
4.90
4
Impurity Area %Signal-to Noise
Impurity 1 0.006 53
Impurity 2 0.011 70
Impurity 2 0.034 213
Impurity 2 0.003 18
RCA 0.018 89
Irbesartan Tablet 99.927 422602
Better analyte information
Dual wavelength capability is standard
on the 2489 UV/Vis Detector. This gives
you the flexibility to simultaneously
monitor absorbance at two user-selectable
wavelengths, resulting in more analyte
information per run and increased
productivity. The two wavelength channels can
be overlaid, ratioed, and/or summarized
within the data system for better
qualitative data.
Sample: Orange drink mix (unsweetened) 4.2 g in 250 mL of waterColumn: XBridge™ C18, 4.6 x 150 mm, 3.5 µm
Dual wavelength capability allows sensitivity and selectivity to be optimized for each analyte in a single run without the need for wavelength switching.
optimal performance
Whether you work in Research and
Development, Analytical Development,
or QA/QC, the 2489 UV/Vis Detector is
designed to meet your needs with its wide
range of flow cell options and integrated
features, while providing optimal
flexibility, usability, and performance for
both routine and complex samples.
Sample: 7 Ginseng std mix ~ 0.075 mg/mLColumn: XBridge Phenyl, 4.6 x 250 mm, 3.5 µm
As shown in this example, compounds found in natural products that exhibit weak UV absorption characteristics, such as ginsenosides found in Panax ginseng, are easily detected due to the superior sensitivity of the 2489 UV/Vis Detector.
Lamp optimization ensures consistent noise performance for high sensitivity applications and reproducible integration whether your lamp is new, right out-of-the-box, or over 2000+ hours.
Sample: Caffeine and acetaminophen, approx. 0.0025 mg/mL Column: Atlantis dC18, 3.0 x 150 mm, 3 μmDetection: 270 nm, 5 Hz, 0.5 s
Waters Corporation 34 Maple Street Milford, MA 01757 U.S.A. T: 1 508 478 2000 F: 1 508 872 1990 www.waters.com
Waters, Alliance, Empower, MassLynx, and Atlantis are registered trademarks of Waters Corporation. Intelligent Speed, SunFire, TaperSlit, XBridge, XSelect, and The Science of What’s Possible are trademarks of Waters Corporation. All other trademarks are the property of their respective owners.