FULL PROGRAMME Saturday 23 September: Optional Pre-conference programme Pre-conference training course This course is fully booked In this training course participants will have the opportunity to gain hands-on experience with the latest open- source, freely available, solutions for standardized cytometry data management, integration and analysis. Location: Conference venue. Registration fee: € 60 when you attend the Conference; € 95 for the course only without attending the Conference. Maximum 30 delegates; pre-registration required via the online registration form 14.00-18.30 TRAINING COURSE ON CYTOMETRY ANALYSIS TOOLS DOCK SIX II 14.00-16.00 Instructor: Iannis Drakos (Paris, FR) A brief introduction in the Flow Cytometry Standard (FCS) Quality control and quality assessment Normalization Data cleanup (removing low quality/corrupted events or samples) 16.00-16.30 Coffee break 16.30-18.30 Instructor: Nicolas Derian (Paris, FR) Cell population identification (gating) Cell population annotation (labeling the gates) Diagnosis ESCCA European Cytometry Certification Examination This exam will be held from 17.00-19.00 hrs. at the Conference venue. Candidates will be presented with some 100 multiple choice questions on a wide range of topics. At least 60% of the questions have to be answered correctly in order to pass the exam and receive the ESCCA Certificate. The exam is only available to ESCCA members. Location: Conference venue Admission fee: € 100 For more information and the application procedure, please see the membership section of the ESCCA website. Restricted to ESCCA members; maximum 30 places. For more information and registration, please see the Membership section of the ESCCA website. Pre-registration for the Conference The registration desk is open from 17.00-19.30 hrs. on Saturday. Please collect your badge and registration materials while enjoying a welcome drink and meeting and greeting your fellow participants. For the opening hours during the rest of the Conference, please consult the General Information page of the website. ESCCA 2017 ● THESSALONIKI 24-27 SEPTEMBER 2017
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FULL PROGRAMME Saturday 23 September: Optional Pre-conference programme Pre-conference training course This course is fully booked In this training course participants will have the opportunity to gain hands-on experience with the latest open-source, freely available, solutions for standardized cytometry data management, integration and analysis. Location: Conference venue. Registration fee: € 60 when you attend the Conference; € 95 for the course only without attending the Conference. Maximum 30 delegates; pre-registration required via the online registration form
14.00-18.30 TRAINING COURSE ON CYTOMETRY ANALYSIS TOOLS
DOCK SIX II
14.00-16.00 Instructor: Iannis Drakos (Paris, FR)
A brief introduction in the Flow Cytometry Standard (FCS)
Quality control and quality assessment
Normalization
Data cleanup (removing low quality/corrupted events or samples)
16.00-16.30 Coffee break
16.30-18.30 Instructor: Nicolas Derian (Paris, FR)
Cell population identification (gating)
Cell population annotation (labeling the gates)
Diagnosis
ESCCA European Cytometry Certification Examination
This exam will be held from 17.00-19.00 hrs. at the Conference venue. Candidates will be presented with some 100 multiple choice questions on a wide range of topics. At least 60% of the questions have to be answered correctly in order to pass the exam and receive the ESCCA Certificate. The exam is only available to ESCCA members. Location: Conference venue Admission fee: € 100 For more information and the application procedure, please see the membership section of the ESCCA website.
Restricted to ESCCA members; maximum 30 places. For more information and registration, please see the Membership section of the ESCCA website. Pre-registration for the Conference The registration desk is open from 17.00-19.30 hrs. on Saturday. Please collect your badge and registration materials while enjoying a welcome drink and meeting and greeting your fellow participants. For the opening hours during the rest of the Conference, please consult the General Information page of the website.
Chairs: Katherina Psarra (Athens, GR), Alexandra Fleva (Thessaloniki, GR)
CRYSTAL HALL
09.00-09.10 Welcome address & announcements - Welcome from the ESCCA President: Katherina Psarra (Athens, GR)
09.10-09.25 Thessaloniki: a city at the crossroads of film cultures
Yannis Lambrou (Athens, GR)
09.25-10.15 PLENARY 1 KEYNOTE LECTURES
Chairs: Stefano Papa (Urbino, IT), Claudio Ortolani (Urbino, IT)
CRYSTAL HALL
09.25-09.50
PLE-01-01 2016: WHO update: acute leukemias Mike Borowitz (Baltimore, MD, USA)
09.50-10.15
PLE-01-02 2016: WHO update: mature lymphoid malignancies Andy Rawstron (Leeds, UK)
10.15-11.00 ESCCA INDUSTRIAL PARTNER PRESENTATION
CRYSTAL HALL
11.00-11.30 BREAK
11.30-13.00 A1 ADVANCED HEMATOLOGY-ONCOLOGY
Chairs: Andy Rawstron (Leeds, UK), Katherina Psarra (Athens, GR)
CRYSTAL HALL
11.30-12.15 Normal and malignant myelopoiesis Frank Preijers (Nijmegen, NL)
12.15-13.00 AML Dx & MRD Gerrit Jan Schuurhuis (Amsterdam, NL)
11.30-13.00 B1 Group 1
CLINICAL DIAGNOSTIC CYTOMETRY
Moderator: Claudio Ortolani (Urbino, IT)
DOCK SIX I
Maximum number of participants is 80, divided over 2 groups. Your group number is indicated on your badge. Please note that in view of the interactive sessions it is not possible to switch groups.
11.30-12.15 Clinical flow overview Bruno Brando (Legnano, IT)
12.15-13.00 PNH cases Steve Richards (Leeds, UK)
Sunday 24 September continued
11.30-13.00 B1 Group 2
CLINICAL DIAGNOSTIC CYTOMETRY
Moderator: Enrique O’Connor (Valencia, ES)
DOCK SIX II
Your group number is indicated on your badge. Please note that in view of the interactive sessions it is not possible to switch groups on-site.
11.30-12.15 PNH cases Steve Richards (Leeds, UK)
12.15-13.00 Clinical flow overview Bruno Brando (Legnano, IT)
16.30-18.00 C3 ADVANCED IMMUNOLOGY: HLA TYPING AND CROSS-MATCH
Chairs: Silvia Della Bella (Milan, IT), Genny del Zotto (Genoa, IT)
TIMBER HALL II
16.30-17.15 HLA Single Antigen Typing Bruno Brando (Legnano, IT)
17.15-18.00 PRA and HLA Cross-Match Katherina Psarra (Athens, GR)
MONDAY 25 SEPTEMBER
09.00-10.30 A4 ADVANCED HEMATOLOGY-ONCOLOGY
Chairs: Andy Rawstron (Leeds, UK), Katherina Psarra (Athens, GR)
CRYSTAL HALL
09.00-09.45 Normal T-cell subsets Hyun-Dong Chang (Berlin, DE)
09.45-10.30 Refining the diagnosis of clonal disorders of Large Granular Lymphocytes Julia Almeida (Salamanca, ES)
09.00-10.30 B4 Group 1
CLINICAL DIAGNOSTIC CYTOMETRY
Moderator: Claudio Ortolani (Urbino, IT)
DOCK SIX I
09.00-09.45 ALL Mike Borowitz (Baltimore, MD, USA)
09.45-10.30
AML Gerrit Jan Schuurhuis (Amsterdam, NL)
09.00-10.30 B4 Group 2
CLINICAL DIAGNOSTIC CYTOMETRY
Moderator: Enrique O’Connor (Valencia, ES)
DOCK SIX II
09.00-09.45 MDS Anna Porwit (Lund, SE)
09.45-10.30
Myeloproliferative neoplasms and acute leukemia: focus on CMML and monocytic-lineage leukemias Sergio Matarraz (Salamanca, ES)
09.00-10.30 C4 ADVANCED IMMUNOLOGY: mRNA AND miRNA DETECTION
Chairs: Silvia Della Bella (Milan, IT), Genny del Zotto (Genoa, IT)
TIMBER HALL II
09.00-09.45 RNA detection by flow cytometry Gloria Martrus (Hamburg, DE)
09.45-10.30 miRNA in DC vaccination Willemijn Hobo (Nijmegen, NL)
10.30-11.00 COFFEE BREAK - EXHIBITION - POSTERS
11.00-12.30 PLENARY 2 ESCCA INFORMATICS PROJECTS
Chairs: Attila Tarnok (Leipzig, DE), Katherina Psarra (Athens, GR)
CRYSTAL HALL
11.00-11.30 PLE-02-01 Health-RI: a next generation of research infrastructure to improve translation from biology to clinic Mariska Bierkens (Amsterdam, NL)
11.30-12.00 PLE-02-02 Daedalus: an ESCCA project to elevate the impact of cytomics on translational research Iannis Drakos and Nicolas Derian (Paris, FR)
12.00-12.30 PLE-02-03 Working with ESCCAbase Claudio Ortolani (Urbino, IT)
Monday 25 September continued
12.30-13.15 ESCCA INDUSTRIAL PARTNER PRESENTATION
CRYSTAL HALL
13.15-14.30 LUNCH BREAK - EXHIBITION - POSTERS
14.30-16.00 A5 ADVANCED HEMATOLOGY-ONCOLOGY
Chairs: Andy Rawstron (Leeds, UK), Katherina Psarra (Athens, GR)
CRYSTAL HALL
14.30-15.15 B-lymphoproliferative disorders Andy Rawstron (Leeds, UK)
15.15-16.00 Plasma Cell Disorders Mikhail Roshal (New York, USA)
14.30-16.00 B5 Group 1
CLINICAL DIAGNOSTIC CYTOMETRY
Moderator: Claudio Ortolani (Urbino, IT)
DOCK SIX I
14.30-15.15 MDS Anna Porwit (Lund, SE)
15.15-16.00 Myeloproliferative neoplasms and acute leukemia: focus on CMML and monocytic-lineage leukemias Sergio Matarraz (Salamanca, ES)
14.30-16.00 B5 Group 2
CLINICAL DIAGNOSTIC CYTOMETRY
Moderator: Enrique O’Connor (Valencia, ES)
DOCK SIX II
14.30-15.15 ALL Mike Borowitz (Baltimore, MD, USA)
15.15-16.00 AML Gerrit Jan Schuurhuis (Amsterdam, NL)
Chairs: Silvia Della Bella (Milan, IT), Genny del Zotto (Genoa, IT)
TIMBER HALL II
16.30-17.15 Emerging frontiers in flow cytometry detection of extracellular vesicles Paola Lanuti (Chieti, IT)
17.15-18.00 Detecting cell-derived vesicles with cell counters: from artefact to clinical act Edwin van der Pol (Amsterdam, NL)
18.30-23.00 18.15-18.45 18.45-23.30
GREEK SUNSET DINNER PARTY Pre-registration required; a fee applies. For more information, please click here. Bus transfer from the Porto Palace Hotel Greek Sunset Dinner Party
12.00-12.15 PLE-04-02 The PD-1 immune checkpoint on human NK cells in normal and pathological condition Silvia Pesce (Genoa, IT)
12.15-13.00 PLENARY 5 BEST POSTER ABSTRACT PRESENTATIONS
Chairs: Jordi Petriz (Barcelona, ES), Stefano Papa (Urbino, IT)
CRYSTAL HALL
12.15-12.22 PLE-05-01 Poster # 55
Leukemic stem cells detection in PB by flow cytometry: a simple and rapid new diagnostic tool for Chronic Myeloid Leukemia Santina Sirianni (Siena, IT)
12.22-12.29 PLE-05-02 Poster # 33
Probabilistic models for automated flow cytometric analysis of minimal residual disease in chronic lymphocytic leukemia Konstantia Kotta
12.29-12.36 PLE-05-03 Poster # 56
CD73, an ecto-5’-nucleotidase, is a commonly overexpressed aberrant marker in Acute Leukaemias Mahima Sanyal (Mumbai, IN)
to be continued on the next page
Tuesday 26 September continued
Plenary 5 – Best Poster Abstract Presentations – continued
12.36-12.43 PLE-05-04 Poster # 57
Identification of Minimal Disseminated Disease in T-Cell Acute Lymphoblastic Lymphoma by Flowcytometric Immunophenotyping Ganesh Kumar Viswanathan (Mumbai, IN)
12.43-12.50 PLE-05-05 Poster # 58
Multicenter study of the antibody VS38c, at diagnosis, MRD and patients undergoing Daratumumab treatment for Multiple Myeloma (MM) Ricardo Morilla
12.50-12.57 PLE-05-06 Poster # 59
CD44 is highly expressed in adult B-cell precursor Acute Lymphoblastic Leukemia and is a useful minimal residual disease monitoring marker Pearl Rodrigues (Mumbai, IN)
13.00-14.30 13.00-13.15
LUNCH BREAK - EXHIBITION - POSTERS Exhibition Raffle prize drawing in the Crystal Hall
Chairs: Attila Tarnok (Leipzig, DE), Alexandra Fleva (Thessaloniki, GR)
CRYSTAL HALL
14.30-15.00 PAR-01-01 The different facets of myeloid-derived suppressor cells in cancer patients Susanna Mandruzzato (Padova, IT)
15.00-15.30 PAR-01-02 Pitfalls in automated gating of Lymphocyte Populations in HIV patients Maria Arroz (Lisbon, PT)
15.30-15.45 PAR-01-03 Selected oral abstract presentation: Monitoring of circulating Plasmablasts in IgG4 Related Disease Raffaella Milani (Milan, IT)
15.45-16.00 PAR-01-04 Selected oral abstract presentation: Predictive/prognostic value of myeloid-derived suppressor cells and regulatory T cell subsets in untreated non-small cell lung cancer patients Eleni-Kyriaki Vetsika (Heraklion, GR)
14.30-16.00 PARALLEL 2 NEW CAPABILITIES FOR FLOW CYTOMETRY Chairs: Jordi Petriz (Barcelona, ES), George Vartholomatos (Ioannina, GR)
TIMBER HALL II
14.30-14.50 PAR-02-01 Cancer stem cells and mechanisms of multidrug resistance by flow cytometry Jordi Petriz (Barcelona, ES)
14.50-15.15 PAR-02-02 Principles of acoustic focusing cytometry and application to rare population analysis Michael Ward (Eugene, OR, USA)
15.15-15.30 PAR-02-03 Selected oral abstract presentation: Detection of circulating cell-derived microparticles across different cardiovascular risk groups Eugenia Gkaliagkousi (Thessaloniki, GR)
to be continued on the next page
Tuesday 26 September continued
Parallel 3 – New capabilities for flow cytometry – continued
15.30-15.45 PAR-02-04 Selected oral abstract presentation: Application of a new analysis strategy including recent multidimensional software to hematological multiparametric flow cytometry data Marie-Christine Béné (Nantes, FR)
15.45-16.00 PAR-02-05 Selected oral abstract presentation: Utility of CD148, CD180 & CD200 in the differential diagnosis of B-cell non-Hodgkin lymphoma involving peripheral blood and bone marrow Prashant Tembhare (Navi Mumbai, IN)
14.30-16.00 PARALLEL 3 UK NEQAS LI FOCUS SESSION
Chairs: Liam Whitby (Sheffield, UK) and Stefano Papa (Urbino, IT)
DOCK SIX II
14.30-15.00 PAR-03-01 Technical and analytical error in Clinical Flow Cytometry Liam Whitby (Sheffield, UK)
15.00-15.30 PAR-03-02 Harmonisation of leukaemia reporting: Is it achievable? Alison Whitby (Sheffield, UK)
15.30-16.00 PAR-03-03 Findings of the Haematological Malignancy Bone Marrow Aspirate Assessment Pre-Pilot programme Liam Whitby (Sheffield, UK)
16.00-16.30 COFFEE BREAK - EXHIBITION - POSTERS
16.30-18.00 PLENARY 6 PRELEUKEMIC STATES
Chairs: Frank Preijers (Nijmegen, NL), Aglaia Dimitrakopoulou (Athens, GR)
Chairs: Paula Fernandez (Aarau, CH), Aglaia Dimitrakopoulou (Athens, GR)
CRYSTAL HALL
09.00-09.20 PAR-04-01 Introductory lecture: Normal and malignant mast cells
Cristina Teodosio (Leiden, NL)
09.20-09.40 PAR-04-02 Eosinophilia: The role of flow cytometry George Paterakis (Athens, GR)
09.40-09.55 PAR-04-03 Selected oral abstract presentation: Characterization of redox signaling sensitivity associated with leukemia disease progression by phospho-specific flow cytometry Maria Teresa Scupoli (Verona, IT)
09.55-10.10 PAR-04-04 Selected oral abstract presentation: Accumulation of classical monocytes defines a subgroup of MDS that frequently evolve into CMML Orianne Wagner-Ballon (Créteil, FR)
10.10-10.25 PAR-04-05 BEST ORAL ABSTRACT NOMINEE: Co-expression of CD25/CD34/CD123 is a highly predictive indicator of FLT3-ITD mutations in acute myeloid leukemia Manisha Suthar (Navi Mumbai, IN)
10.25-10.30
Q&A
09.00-10.30 PARALLEL 5 FLOW CYTOMETRY IN CLINICAL TRIALS
Chairs: Frank Preijers (Nijmegen, NL), Alexandra Fleva (Thessaloniki, GR)
TIMBER HALL II
09.00-09.20 PAR-05-01 Challenges of flow Cytometry in Global Clinical studies - I Ioanna Athanasiadou (Geneva, CH)
09.20-09.40 PAR-05-02 Challenges of flow Cytometry in Global Clinical studies - II Christèle Gonneau (Geneva, CH)
09.40-09.55 PAR-05-03 BEST ORAL ABSTRACT NOMINEE: Association between the expression of factor XIII-A in lymphoblasts and the ’B-other’ cytogenetic group in children with acute lymphoblastic leukemia Zsuzsanna Hevessy (Debrecen, HU)
09.55-10.10 PAR-05-04 BEST ORAL ABSTRACT NOMINEE: Minimal residual disease and log –reduction explain the superior value of double autologous stem cell transplant in multiple myeloma Giovanni Rossi (San Giovanni Rotondo, IT)
10.10-10.25 PAR-05-05 Selected oral abstract presentation: Prognostic value of flow cytometric bone marrow investigation in neuroblastoma patients Alexander Popov (Moscow, RU)
10.25-10.30
Q&A
Wednesday 27 September continued
09.00-10.30 PARALLEL 6 QUALITY CONTROL AND DATA ANALYSIS
09.40-09.55 PAR-06-03 Selected oral abstract presentation: TERS - application of a new tool for standardizing functional T-cell assays Florian Kern (Brighton, UK)
09.55-10.10 PAR-06-04 Selected oral abstract presentation: Evaluation of CD319 (SLAMF7) as a novel gating marker for plasma cells in flow cytometric immunophenotyping of multiple myeloma Sitaram Ghogale (Mumbai, IN)
10.25-10.30
PAR-06-05 Selected oral abstract presentation: AML MRD by multiparameter flow cytometry using LAIP and LSC: Methodological aspects in multicentric study of the French AML Intergroup Adriana Plesa (Lyon, FR)
10.25-10.30
Q&A
10.30-11.00 COFFEE BREAK - POSTERS
11.00-12.15
PLENARY 7 STANDARDISATION VS. HARMONISATION
Chairs: Stefano Papa (Urbino, IT), Frank Preijers (Nijmegen, NL)
CRYSTAL HALL
11.00-11.15 PLE-07-01 ESCCA Harmonization Projects Andy Rawstron (Leeds, UK)
11.15-11.45
PLE-07-02
B-ALL Euroflow Vincent van der Velden (Rotterdam, NL)
12.15-12.40 Clinical Cytometry Best Original Paper Award 2016-2017 Single cell analysis of human tissues and solid tumors with mass cytometry Jonathan M. Irish, Nalin Leelatian (Nashville, TN, USA)
12.40-12.55 ESCCA Best Oral Abstract Award Nominations for the Award are (in alphabetical order):
Zsuzsanna Hevessy (Debrecen, HU) Association between the expression of factor XIII-A in lymphoblasts and the ’B-other’ cytogenetic group in children with acute lymphoblastic leukemia (presented in PAR-05-03)
Giovanni Rossi (San Giovanni Rotondo, IT) Minimal residual disease and log –reduction explain the superior value of double autologous stem cell transplant in multiple myeloma (presented in PAR-05-04)
Manisha Suthar (Navi Mumbai, IND) Co-expression of CD25/CD34/CD123 is a highly predictive indicator of FLT3-ITD mutations in acute myeloid leukemia (presented in PAR-04-05)
12.55-13.05 ESCCA Best Poster Award The Scientific Committee will review the posters on-site and select the Best
Poster.
13.05-13.10 Presentation of ESCCA 2018
13.10-14.00 LUNCH BREAK - POSTERS
14.00-15.30 PLENARY 9 INTERESTING CASES WITH INTERACTIVE VOTING
Chair: George Paterakis (Athens, GR), Aglaia Dimitrakopoulou (Athens, GR)
CRYSTAL HALL
In this session a series of clinical cases will be presented. Together with the presenter these cases will be actively reviewed by the delegates through interactive voting via your mobile phone. The session includes:
voting on an initial differential diagnosis based on CBC and morphology;
presentation of flow cytometry data; voting on a possible diagnosis or possible cytogenetics suspected;
presentation of the diagnostic data and re-voting on the diagnostic options;
review of the answers.
15.30-16.00 BREAK
16.00-17.30 PLENARY 10 IMMUNOTHERAPY IN HEAMATOLOGICAL MALIGNANCIES