Accepted Manuscript Epidemiology of human coronavirus NL63 infection among hospitalized patients with pneumonia in Taiwan Su-Hua Huang, Mei-Chi Su, Ni Tien, Chien-Jhen Huang, Yu-Ching Lan, Chen-Sheng Lin, Chao-Hsien Chen, Cheng-Wen Lin, PhD, Professor PII: S1684-1182(15)00905-6 DOI: 10.1016/j.jmii.2015.10.008 Reference: JMII 713 To appear in: Journal of Microbiology, Immunology and Infection Received Date: 28 July 2015 Revised Date: 18 September 2015 Accepted Date: 22 October 2015 Please cite this article as: Huang S-H, Su M-C, Tien N, Huang C-J, Lan Y-C, Lin C-S, Chen C-H, Lin C- W, Epidemiology of human coronavirus NL63 infection among hospitalized patients with pneumonia in Taiwan, Journal of Microbiology, Immunology and Infection (2015), doi: 10.1016/j.jmii.2015.10.008. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
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Accepted Manuscript
Epidemiology of human coronavirus NL63 infection among hospitalized patients withpneumonia in Taiwan
Su-Hua Huang, Mei-Chi Su, Ni Tien, Chien-Jhen Huang, Yu-Ching Lan, Chen-ShengLin, Chao-Hsien Chen, Cheng-Wen Lin, PhD, Professor
PII: S1684-1182(15)00905-6
DOI: 10.1016/j.jmii.2015.10.008
Reference: JMII 713
To appear in: Journal of Microbiology, Immunology and Infection
Received Date: 28 July 2015
Revised Date: 18 September 2015
Accepted Date: 22 October 2015
Please cite this article as: Huang S-H, Su M-C, Tien N, Huang C-J, Lan Y-C, Lin C-S, Chen C-H, Lin C-W, Epidemiology of human coronavirus NL63 infection among hospitalized patients with pneumonia inTaiwan, Journal of Microbiology, Immunology and Infection (2015), doi: 10.1016/j.jmii.2015.10.008.
This is a PDF file of an unedited manuscript that has been accepted for publication. As a service toour customers we are providing this early version of the manuscript. The manuscript will undergocopyediting, typesetting, and review of the resulting proof before it is published in its final form. Pleasenote that during the production process errors may be discovered which could affect the content, and alllegal disclaimers that apply to the journal pertain.
Epidemiology of human coronavirus NL63 infection among hospitalized patients with 3
pneumonia in Taiwan 4
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Su-Hua Huang1 Mei-Chi Su2 Ni Tien2 Chien-Jhen Huang3 7
Yu-Ching Lan4 Chen-Sheng Lin5 Chao-Hsien Chen3 Cheng-Wen Lin 1, 3* 8 9 1Department of Biotechnology, Asia University, Wufeng, Taichung, Taiwan. 10 2Department of Laboratory Medicine, China Medical University Hospital, Taichung, 11
Taiwan. 12 3Department of Medical Laboratory Science and Biotechnology, China Medical 13
University, Taichung, Taiwan. 14 4Department of Health Risk Management, School of Public, China Medical 15
University, Taichung, Taiwan. 16 5Division of Gastroenterology, Kuang Tien General Hospital, Taichung, Taiwan 17
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Short title: HCoV-NL63 infection in Taiwan 19
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*Corresponding author. 25
Mailing address: Cheng-Wen Lin, PhD, Professor. Department of Medical Laboratory 26
Science and Biotechnology, China Medical University, No. 91, Hsueh-Shih Road, 27
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Detection of human coronavirus-NL63 in children in Japan. Pediatr Infect Dis J 310
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pandemic influenza A/H1N1 virus during 2009-2010 in Taiwan. Virus Res 2013; 329
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177: 46-54. 330
15. Van der Hoek L, Pyrc K, Jebbink MF, Vermeulen-Oost W, Berkhout RJ, Wolthers 331
KC, et al. Identification of a new human coronavirus. Nat Med 2004; 10: 368-73. 332
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Figure captions 344
Figure 1. Age distribution HCoV-NL63 (A-B) and influenza virus (C-D) infection 345
in hospitalized patients with pneumonia (A, C) and outpatients with 346
influenza-like illness (B, D). 347
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Figure 2. Seasonal prevalence of HCoV-NL63, influenza A/H1N1, A/H3N2 and B 349
viruses during 2010-2011 in Taiwan. Positive numbers (Left) and percentage 350
(Right) of HCoV-NL63 infection in hospitalized patients (A), as well as influenza 351
A/B infection in hospitalized patients (B) and outpatients (C). 352
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Figure 3. Phylogenetic tree of partial 1a gene sequences from Taiwan and global 354
HCoV-NL63 strains using Maximum-likehood method. Phylogenetic tree 355
plots nucleotide sequences of HCoV-NL63 strains in this study and worldwide 356
strains. Sequences were aligned via BioEdit and Clustal_X, phylograms 357
generated by ML methods and MEGA tree-drawing software. Branch labels 358
represent stability of branches over 1,000 bootstrap replicates, only bootstrap 359
values >60% presented. 360
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Table 1. Clinical data of hospitalized patients with pneumonia in this study.
*Negative for real-time PCR detection of HCoV NL63, influenza A/H1N1, influenza A/H3N2, and influenza B viruses. + Patients co-infected with HCoV NL63 and influenza A/H1N1. aP value = 0.037 (Negative or Flu A/H1N1 positive vs co-infection); bP value <0.02 (Negative, HCoV NL63 positive or Flu A/H1N1 positive vs co-infection)
Hospitalized patients with pneumonia
Negative* HCoV NL63 positive Flu A/H1N1
positive Flu A/H3N2
positive Flu B positive HCoV NL63 positive/
Flu A/H1N1 positive+ Cases 70 9 17 9 5 3
Average age (Y) 37.3 41.7 42.4 56.1 45.6 58.7
Clinical symptom (%)
Breathing difficulties 48.6% 55.6% 52.9% 33.3% 60.0% 66.7% a
Table 2. Clinical data of the outpatients with influenza-like illness in this study.
*Negative for real-time PCR detection of HCoV NL63, influenza A/H1N1, influenza A/H3N2, and influenza B viruses + Two patients co-infected with HCoV NL63 and influenza A/H1N1 and one co-infected with HCoV NL63 and influenza A/H3N2 aP value <0.02 (negative or Flu A/H1N1 positive vs Flu A/H3N2 positive)