2013 HID University Introduction

1 Life Technologies™ |

Introduction: Increasing Success Rates & Efficiency from Crime Scene to Courtroom Ariana Wheaton Senior Market Development Manager - Americas Life Technologies


Increased Efficiency

Ease of Use Success with Difficult Samples

Simplified Interpreta;on

Higher Throughput

Streamlined Workflow

Technology Improvements Over Last 7 Years…

Increased Integra9on and Efficiency


Time to Result

(hrs/per 24 samples) ~1-‐3.5 hrs ~0-‐2 hrs ~4 hrs ~1.5 hrs ~1 hrs

Improved Efficiency for Single Source Sample Processing

Time to Result

(per 24 samples) 0 0 ~40 mins ~35 mins ~5-‐10 mins

Total Time to Result = ~7-‐12 hrs

Collec;on Extrac;on Quan;ta;on/ Normaliza;on Amplifica;on Detec;on Analysis

Total Time to Result = ~1.5 hrs



Sample Prepara;on

Quan;ta;on/ Normaliza;on Amplifica;on Detec;on Analysis

Time to Result

(hrs/per 6-‐16 samples) ~1-‐48 hrs ~2 hrs ~3.5 hrs ~0.75 hrs ~2-‐3 hrs Time to Result

(hrs/per 13 samples) ~1-‐3 hrs ~1.5 hrs ~1.5 hrs ~0.55 hrs ~1-‐2 hrs


Dependent on sample type and method

Improved Efficiency for Casework Sample Processing


A lot of progress… But how much have we increased your casework success rates?

Increased Efficiency

Ease of Use Success with Difficult Samples

Simplified Interpreta;on

Higher Throughput

Streamlined Workflow

Technology Improvements Over Last 7 Years…


Results from recent Forensic Magazine survey… Failure rate following Amplification/CE

Overall average from North American Crime Labs (n=126)

§  20% - Note: Labs processing higher % of property crimes and touch DNA have much higher failure rates.

Ø  We did a similar survey in 2006, and the average failure rate was also 20%!

Ø  Survey indicated failure rates have not been reduced, but this is due to increase in property crime processing and more challenging samples (i.e. touch samples)


What is the cost of failed samples?

§  2006 study* in the USA estimates processing a single piece of evidence through a DNA workflow takes on average: §  157.6 minutes of analyst and labor time §  Anywhere between $425 and $1,720

§  In addition, outsourcing labs typically charge the same flat fee for service whether or not the submitted sample yields probative or useful information

*Source: Cost Study of DNA Testing and Analysis North Carolina DOJ OSBM March 2006

Increasing success rates in the lab means more efficient use of investigative and laboratory resources


What contributes to the total processing cost for a DNA evidence sample?

0%

10%

20%

30%

40%

50%

60%

70%

80%

90%

100%

Typical Cost Breakout*

Labor Cost/Time Facility Overhead Shipping Reagents/Supplies

Most impacted by 1st Pass Success Rate

*Actual cost breakout will vary by laboratory


The “Hidden Cost” of Low First Pass Success Rates

Higher 1st Pass Success Rate = Less Re-‐runs

Lower 1st Pass Success Rate = More Re-‐runs

Reduced Labor & Consumable Cost

Increased Labor & Consumable Cost


Detection & Analysis

Our Mission: Improve Efficiency. Increase Success Rates. Provide More Info.

LIFE Offers Complete Integrated HID Workflow

GeneMapper® ID-X Expert System

Software

Next Generation STR Kits

3500/3500xL Genetic Analyzer

3130/3130xl Genetic Analyzer

Extraction Quantification Amplification

7500 Real-Time PCR Instrument

Next Generation Quantifiler® Kits

PrepFiler™ Forensic DNA Extraction kit

Veriti® 96-Well Thermal Cycler

GeneAmp® PCR System 9700 Cycler

AutoMate Express™

Collection

Copan 4N6FLOQSwab™

& NUCLEIC-CARD™

Screening

ParaDNA®

Converge™ Software DNA Workflow

Kinship and Paternity

Direct Search Familial Search

NEW

NEW

NEW

NEW

Converge™

Software

NEW

NEW


Strategies for Increased Downstream Success Rates:

Improve Collection & Sample Selection Collection

4N6FLOQSwabs™ Patented technology to maximize DNA

collection and elution efficiency; enables significantly improved profile success

rates compared to cotton swabs

Screening Sample to Answer Casework Screening Device to help determine which samples

contain the highest likelihood of generating a probative DNA profile in a

laboratory workflow


NUCLEIC-CARD™ System—Now Available Globally

§  Scheduled to A reliable device for collection, preservation, and long-term storage of nucleic acids

Chemically-treated matrix to enable cell lysis and protein denaturation

NUCLEIC –CARD™ NUCLEIC –CARD™ COLOR

NUCLEIC –CARD™ Collection Device


Quantification – Areas for Improvement

Predic9on of M:F Mixture Ra9o

Improve Sensi9vity and Reproducibility

Predict Sample Quality

Match Performance to Newer Genera9on STR kits

Improve DNA Standard Consistency

Faster Time to Result


Quantifiler® Kits Product Family

Quantifiler® Duo Kit Quantifiler® Human Kit

Quantifiler® Y Human Kit

Three kits – for different applications • Quantifies DNA from wide variety of sources • Works with wide variety of DNA input • Sample Extraction chemistry compatible • Guides selection of optimal AmpFSTR®

PCR Amplification Kit • Accurate, Sensitive & Specific

Next Gen Quantification

Two new Quantifiler® Kits in 2013 +

Flexible workflow software

To integrate with Next Generation STR kits

Quantifiler® HP Kit Quantifiler® Trio Kit

HID RT v1.2 Software

NEW


The GlobalFiler™ Kit: Going Further for Casework


Additional 6-dye Application: Yfiler® Plus Kit


Fall 2013

Yfiler® Plus: The First 6-Dye, 27 Marker Y-STR Multiplex

Plus in Power of Discrimination

•  All 17 loci amplified by the Yfiler® kit •  10 new Y-STR loci including 7 Rapidly Mutating1 Y-STRs for ability to

discriminate between close male relatives •  11 mini STRs below 220bp

Plus in Performance

•  Improved sensitivity & results with challenging casework samples •  Improved recovery of alleles and intracolor balance in male/female

mixtures containing high female background •  Improved resolution capacity for male/male mixtures •  Expanded allelic ladder •  Shorter amplification times •  Direct amplification protocol with single-source samples

1.  Rapidly Mutating Y-STRs have mutation rates above 1 x 10-2 per marker per generation. 17 Yfiler Y-STRs show mutation rates between 1 x 10-3 and 1 x 10-4 per marker per generation. The content provided herein may relate to products that have not been officially released and is subject to change without notice.


Ion Torrent™ Semiconductor Sequencing for Forensic Applications

Casework Single Source


Mitochondrial DNA Sequencing

SNP Genotyping Paternity, Mixtures, Missing

Persons, DVI

Ancestral & Phenotypic SNPs for Inves;ga;ve Leads

Eye Color

Hair Color

Missing Persons, DVI Facial Reconstruc;on

Looking ahead… Poten9al PGM™ Human Iden9fica9on Applica9ons


Webinar hosted by Forensic Magazine Ion Torrent™ Semiconductor Sequencing for Forensic Applica9ons

Lisa Calandro Director of Product Management Life Technologies

Dr. Walther Parson Institute of Legal Medicine University of Innsbruck, Austria

Available to view “on-demand”: www1.gotomeeting.com/register/134635184


HID Workflow Software Solutions


Converge™ Software - Integrate & Manage All Your Data, Analysis & Reporting Tools

§  Modular system means easier deployment of new: −  Analysis tools (i.e. Paternity/Kinship, Database & Familial Searching) −  Lab management tools −  Data management tools −  Reporting tools

Long term goal of Converge™ Centralize and integrate data, analysis and reporting across all points of the forensic DNA workflow including:

−  At the crime scene −  At the police agency −  At the crime lab −  In the court room

Converge™


Our Mission: Improve Efficiency. Increase Success Rates. Provide More Info.

More Workflow Efficiency

Higher Success

Rates

More

Information

4N6FLOQSwab™ &

NUCLEIC-CARD™ Collection Devices

ü

ParaDNA® Screening System ü

Next Gen Quantifiler® Kits ü ü ü

GlobalFiler® Kits ü ü ü

Yfiler® Plus Kit ü ü ü

Converge™ Software ü ü

PGM™ for Forensics ü


Today’s Agenda

Most of the presentations will be posted to SlideShare: http://www.slideshare.net/Lifetech_HID


THANK YOU!!

Ariana Wheaton Senior Market Development Manager-Americas Life Technologies Phone: 650.638.6317 Email: [email protected]

FOR FORENSIC OR PATERNITY USE ONLY. ©2013 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation and/or its affiliate(s) or their respective owners. The content provided herein may relate to products that have not been officially released and is subject to change without notice.