20.109 Synthetic Biology Module Lecture #3 Ron Weiss Department of Biological Engineering MIT March 15, 2011 Department of Biological Engineering
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20.109Synthetic Biology Module
Lecture #3
Ron Weiss
Department of Biological Engineering
MIT
March 15, 2011Department of Biological Engineering
DNA synthesis & genetic circuits
*Sampling of systems in publications with experimental circuits
207
2,100 2,7007,500 14,600
32,000
583,0001,080,000
100
1,000
10,000
100,000
1,000,000
1975 1985 1995 2005
Length in
base pa
irs
Year
DNA synthesis Synbio Pubs
DNA synthesis & genetic circuits
*Sampling of systems in publications with experimental circuits
?
207
2,100 2,7007,500 14,600
32,000
583,0001,080,000
100
1,000
10,000
100,000
1,000,000
1975 1985 1995 2005
Length in
base pa
irs
Year
DNA synthesis Circuit size
A collection of reusable parts
System elements:Lambda repressor (cI)
Bacteriophage
Lysis/Lysogeny
PR and PRM Promoters
The Promoters
p. 44-45
Repressor Positive and Negative Control
Cooperative Binding
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