Chemistry Green fluorescent protein, GFP QuickTime™ and a TIFF (Uncompressed) dec are needed to see thi QuickTime™ and a TIFF (Uncompressed) dec are needed to see thi QuickTime™ TIFF (Uncompre are needed to Osamu Shimomura Jellyfish/ bioluminescen ce Purified and characterized GFP (1974) Marty Chalfie Obtained the GFP gene (gfp) clone from Prasher in 1992. Made promoter::GFP And Promoter::GFP::Gene expression vectors to show the When and where genes are expressed. Roger Tsien Figured out how GFP glows Made variants with different colors
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2008 Nobel Prize in Chemistry Green fluorescent protein, GFP Osamu Shimomura Jellyfish/ bioluminescence Purified and characterized GFP (1974) Marty Chalfie.
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2008 Nobel Prize in Chemistry Green fluorescent protein, GFP
QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.
QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.
Osamu Shimomura Jellyfish/bioluminescence
Purified and characterized GFP (1974)
Marty Chalfie
Obtained the GFP gene (gfp) clone from Prasher in 1992.
Made promoter::GFP And Promoter::GFP::Gene expression vectors to show the When and where genes are expressed.
Roger Tsien
Figured out how GFP glows
Made variants with different colors
2008 Nobel Prize in Chemistry the other guys
Douglas Prasher The first person to realize the potential of GFP as a tracer molecule.
Cloned GFP in 1992!
Sergey A. Lukyanov
Identified and cloned GFP-like proteins in corals
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GFP
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JellyfishAequorea victoria
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Aequorea victoria photoorgans
GFP
Figure 2. Fluorescence excitation (full-line curve) and emission (dashed curve) spectra of native GFP from Aequorea victoria (Tsien et al., 1998).
Figure 3. The tertiary structure of GFP, displaying its can-like shape with the α-helix, containing the chromophore, threading up through the can. (Brejc et al., 1997)
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GFP color variants
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Have altered absorbsion/emission spectra.
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QuickTime™ and aTIFF (Uncompressed) decompressor
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QuickTime™ and aTIFF (Uncompressed) decompressorare needed to see this picture.
QuickTime™ and aTIFF (Uncompressed) decompressor
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QuickTime™ and aTIFF (Uncompressed) decompressor
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