April 22, 2004 nanoManipulator Apps Advanced Visualization and Control University of Hamburg, Russ Taylor, Summer ‘04 Slide 1 Science Experiments Enabled by The nanoManipulator Russell M. Taylor II April 22, 2004 nanoManipulator Apps Advanced Visualization and Control University of Hamburg, Russ Taylor, Summer ‘04 Slide 2 Administrative • Course Textbook – One is in the library – The other is on reserve in room F-311 • Do not confuse with my office, which is F-331 • Project Teams and Ideas – Meet in the last several minutes of class – Meeting for all who can attend from 3-5 in F-331 • my office – Schedule future meetings for Thursday afternoon or another time April 22, 2004 nanoManipulator Apps Advanced Visualization and Control University of Hamburg, Russ Taylor, Summer ‘04 Slide 3
17
Embed
2004 04 22 nanomanipulator apps - uni-hamburg.de · 1 April 22, 2004 nanoManipulator Apps Advanced Visualization and Control University of Hamburg, Russ Taylor, Summer ‘04 Slide
This document is posted to help you gain knowledge. Please leave a comment to let me know what you think about it! Share it to your friends and learn new things together.
Transcript
1
April 22, 2004nanoManipulator Apps
Advanced Visualization and ControlUniversity of Hamburg, Russ Taylor, Summer ‘04 Slide 1
Science Experiments Enabled byThe nanoManipulator
Russell M. Taylor II
April 22, 2004nanoManipulator Apps
Advanced Visualization and ControlUniversity of Hamburg, Russ Taylor, Summer ‘04 Slide 2
Administrative
• Course Textbook– One is in the library– The other is on reserve in room F-311
• Do not confuse with my office, which is F-331
• Project Teams and Ideas– Meet in the last several minutes of class– Meeting for all who can attend from 3-5 in F-331
• my office
– Schedule future meetings for Thursday afternoon or another time
April 22, 2004nanoManipulator Apps
Advanced Visualization and ControlUniversity of Hamburg, Russ Taylor, Summer ‘04 Slide 3
2
April 22, 2004nanoManipulator Apps
Advanced Visualization and ControlUniversity of Hamburg, Russ Taylor, Summer ‘04 Slide 4
Atomic Force Microscopy 101
Microscope Microscope TipTip
Tip or Sample Surface scanned back and forthTip or Sample Surface scanned back and forth
MirrorMirror
LASERLASERQuadrantQuadrant
PhotodiodePhotodiode
ComputerComputer
DisplayDisplay
April 22, 2004nanoManipulator Apps
Advanced Visualization and ControlUniversity of Hamburg, Russ Taylor, Summer ‘04 Slide 5
E n d - O n - P u s hSliding
Tip contacts object
Tip on substrateLa
tera
l For
ce
AFM tip
Carbon Nanotube
AFM cantilever twists under “lateral” forces
Lateral Force Measurements during a manipulation
s (nm)
Atomic Force MicroscopyManipulation: Quantitative Nanometer-
Nanonewton Forces and Dynamics
April 22, 2004nanoManipulator Apps
Advanced Visualization and ControlUniversity of Hamburg, Russ Taylor, Summer ‘04 Slide 6
nanoManipulator System
3
April 22, 2004nanoManipulator Apps
Advanced Visualization and ControlUniversity of Hamburg, Russ Taylor, Summer ‘04 Slide 7
• Fibrin fibers: major structural component of clots
• Biomedical importance:• wound healing• heart attacks and strokes
• Disorders are associated with fibrinogen mutations• Some lead to excessive bleeding• Others lead to excessive clotting• Clot morphology is different from wild-type
Tools in Bioscience: Fibrin Fibers
April 22, 2004nanoManipulator Apps
Advanced Visualization and ControlUniversity of Hamburg, Russ Taylor, Summer ‘04 Slide 45
+
Lateral aggregation
thrombin
fibrinogen fibrin Protofibrilformation
Fibrin Fiber Questions:What are the biochemical/structural What are the biochemical/structural
factors that determine clotting quality?factors that determine clotting quality?
•• Measure fiber Measure fiber strength during strength during growthgrowth
•• Compare wild Compare wild type and type and mutant/drug mutant/drug treatedtreated
16
April 22, 2004nanoManipulator Apps
Advanced Visualization and ControlUniversity of Hamburg, Russ Taylor, Summer ‘04 Slide 46
Tip hits fiber
Partial surfaceDetachment
Rupture
Tip
fiber
Ft
Fs
Fr
Late
ral f
orce
Tip position
B
TranslocationDeformation
0
0.5
1
1.5
2
2.5
3
0
1000
2000
3000
4000
5000
6000
0 1000 2000 3000 4000 5000 6000s(nm)
Late
ral f
orce
(nN
)
800 nm
Measurements on Individual Fibers
April 22, 2004nanoManipulator Apps
Advanced Visualization and ControlUniversity of Hamburg, Russ Taylor, Summer ‘04 Slide 47
21 DcFR ⋅=
DcFR ⋅= 2
Solid cylinder:
Thin-walled cylinder:
4.1=b3 ⋅= RcF bR
Best exponential fit:
Young’s modulus
E = 0.4 GPa,Steel: E = 200 GPa
DNA: E = 0.1 GPa
Diameter (nm)0.1
1
400
6008001000
3000
50007000
10 100
Rup
ture
forc
e (n
A) R
upture force (nN)
1 .2
1.4
1.6
1.8
2
2.2
2.4
2.6
2.8
3350 3400 3450 3500 3550
Late
ral F
orce
(nA
)
s (nm )
Quantify fiber strength/structure
April 22, 2004nanoManipulator Apps
Advanced Visualization and ControlUniversity of Hamburg, Russ Taylor, Summer ‘04 Slide 48
17
April 22, 2004nanoManipulator Apps
Advanced Visualization and ControlUniversity of Hamburg, Russ Taylor, Summer ‘04 Slide 49
Credits for non-UNC Inclusions
• The Forms of Carbon: Images from the Internet
• Composite Materials: Stanley Tools• Nanotube strong-man: The Economist• Atomic-scale simulation of bent nanotube: