Elr0Vhqvlqj SKRWRQLF WKHUDSLHV ELR0LPDJLQJ ELR0PDQLSXODWLRQ Biophotonics?? Fusion technologies of photonics and biotechnology and biomedical engineering enabled by information technology Lqirupdwlrq Whfkqrorj| Biophotonics - Imaging: X-ray, OCT, polarimetry, DOT, TIRF, photon migration, endoscopy, confocal microscopy, multiphoton microscopy, multispectral imaging - Biosensing: IR spectroscopy, fluorescence, luminescence, Raman scattering, surface plasmon resonance, polarimetry, evanescent wave-based detection - Laser-tissue interaction: ablation, coagulation, cutting, welding, PDT, optical trapping Advantages of the lightwave technology in biomedical engineering - Non-invasive/minimally invasive techniques (detection based on fiber-optics) - Real-time feedback for clinical diagnostics and visual guidance for surgery - High sensitivity and specificity: single molecule sensing, molecular tagging - Wide range of spatial scales that can be sampled: « ʅm~ cm - Abundance of intrinsic (hemoglobin) and extrinsic (fluorescent protein) chromophores Bio-imaging Optical imaging for early detection and monitoring the progression of a disease Benefits of optical imaging: • Non-invasive or minimally invasive • No harmful radioactivity (vs. radioisotope imaging) • Rapid real-time measurements (vs. MRI) • High spatial resolution (vs. ultrasound) • High sensitivity (vs. x-ray imaging) • Portability and compactness • In vitro, in vivo, and ex vivo specimens References http://microscope.fsu.edu/primer/ http://bama.ua.edu/~hsmithso/class/bsc_656/websites/light.html