14. Chromatography GENERAL CATALOGUE 2010/11bio-plast.net/pdf/Catalogue/Haeberle-lab/14 chromatography.pdfgeneral catalogue 2010/11 ... 14.75 x 45 22 x 38.25 23 x 75.5 22 x 75.5 100
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General informations - vials and capsMaterialsAccording to the high requirements of chemical analyses, especially with regard to reproducibility combined with highdetection sensivity, the container material for the respective samples is of great importance. In general, for this pur-pose vials made from glass are used. The hydrolytic resistance of the glass can be taken as a measure for its chemi-cal inertness. Determination of the hydrolytic resistance and the resulting classification of a glass grade are governedby the German and international industrial standard DIN 12111/ISO 719. Glass grades are classified in hydrolyticclasses .We supply vials from the following classes:1st hydrolytic classGlass grades made from borosilicate, such as Duranfi, Pyrexfi, Fiolaxfi and others belong to this group. Glass of thisclass, which is often called neutral glass, has a very good chemical resistiance towards acid and neutral solutions. Therelatively low alkali content permits good values for the resistance towards alkaline solutions, too. If nothing else isstated, the vials of our programm are made from glass of the 1st hydrolytic class (manufactured in accordance withEu.Ph. VI Ed., U.S.P. XXV Ed., DAB-10, Ph. Jap. 13).3rd hydrolytic class, AR glassGlass of this class, also called soft glass or lime soda glass, has a mediumhydrolytic resistance. For longtime storageof aqueous and especially alkaline-aqueous samples (for example to use them repeatedly) itis not recommended. Nevertheless, it can be used for many analytical applications.We supply the following types of sample bottles:vials with crimp top and corresponding capsscrew thread vials and srew capssealing disks for individual combinations of cap and sealExcept for a few frequently used combi packs, vials and caps can be ordered separately, thus allowing a wide range ofpossible combinations.Advantages of the DIN crimp top:Crimp top vials are available with 3 differnt rim heights: 3 mm, DIN crimp top with 3.6 mm and 4mm. When usingonly one crimper, tedious adjustments for the different heights are necessary. The standardized DIN crimp top avoidsthis problem. The rim height in accordance with DIN 58366 should be 3.6 ±0.2 mm. We supply vials with DIN crimptop for volumes above 5 ml.Except where explicitly mentioned, caps with sealing disks are supplied assembled, i. e. ready-to-use.Seals below the caps are shown for illustration purposes only, and they are pictured upside down.
1 2 3 4MACHEREY-NAGEL
Physical properties of glass gradesParameter1st hydrolytic class 3rd hydrolytic classdensity: 2.64 density: 2.5thermal coefficient of linear expansion (K-1): 60 * 10-7 thermal coefficient of linear expansion (K-1):
85 * 10-7
quenching stability (∆ T in K) in accordance with DIN 52321: 60 quenching stability (∆ T in K) in accordancewith DIN 52321: 42internal pressure resistance (bar) in accordance with DIN 52320: atleast 6 bar internal pressure resistance (bar) in accordan-ce with DIN 52320: at least 6 barTemperature stability of sealing disks and plastic capsshaped butyl rubber disks, center coated with PTFE 120°C (-40°C)natural rubberbutyl rubber 140°Csilicone rubber 190°C (-30°C)PTFE 200°C (-60°C)PE (polyethylene) 250°CPP (polypropylene) 80°CAllowable variation for the thickness of sealing disks: ±0.25 mm. 120 - 130°C
4
3
2
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14. ChromatographyVials/Ampoules, Septa, Caps
GENERAL CATALOGUE 2010/11
E & OE.
Crimp top vials and capsVials with crimp top are injection bottles with rim diameters of 8, 11, 13 or 20mmwhich can be closed with crimp caps or PE caps (PE caps only for 11 and 20mm rimdiameters).Vials with snap ring design with 11mm rim diameter may be closed with PE snap-on caps or crimp caps.Conic vials are vials with tapered inner shape for small sample volumes, which can be closed with crimp caps or PEcaps.Micro inserts are used to reduce the volume of standard sample vials for application with very small sample volu-mes. Vials are closed as usual. As an alternative for small volumes you may use the sample vials with conic innershape (conic vials) mentioned above.Crimp caps can be used for crimp top vials and for vials with snap ring design. They are available with or withoutsealing disks, with centre hole, tear-off middle seal or as tear-off caps. Caps with centre hole are made from alumini-um, aluminium with steel insert or steel. The two latter are magnetic.Additionally we offer a versatile range of sealing disks for crimp caps and PE caps.
1MACHEREY-NAGEL
1
Description Capacity diameter / height rim
o.d. x height
PK Cat. No.
ml mm mmN 8-08, clearN 11-1 HP, clear*N 11-1 HP, clear, with writing patchand inscription*N 11-1 HP, amber, writing patchand inscription*Micro insert for N11-1 HP, taperedMicro inserts for N11-1 HP andSnap-ring tubes, fitted with PPspiral pringVials with snap N 11-1, clear*Vials with snap N 11-1, clear, writing patch*Vials with snap N 11-1, amber, writing patch*Micro inserts for Snap-ring tubes,15mm tip, sharply taperingMicro inserts for Snap-ring tubes, 12mm tipN-13-4, clearN 20-6 PE, clear,conical top edge, rounded baseN 20-20 PE, clear conic rim,rounded bottom edgesfor PE autosamplers, wall 1.2 mm thickN20-20 HP/CTC, clear,flat crimp top, long neck rounded bottomedges for PE / CTC and HP autosamplers
0.801.501.501.500.200.25
1.501.501.500.200.304.006.00
20.00
20.00
8 / 311 / 311 / 311 / 3
--
11 / -11 / -11 / -
--
13 / 3.620 / 4
20 / 4
20 / 3.6
8.2 x 30.011.6 x 32.011.6 x 32.011.6 x 32.05.5 x 30.05.5 x 29.0
11.5 x 32.511.5 x 32.511.5 x 32.55.5 x 30.05.5 x 30.014.75 x 4522 x 38.25
*wide neckSnap ring vials N11 can also be used with crimp caps N 11
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1171
14. ChromatographyVials/Ampoules, Septa, Caps
GENERAL CATALOGUE 2010/11
E & OE.
Crimp top vials and capsVials with crimp top are injection bottles with rim diameters of 8, 11, 13 or 20 mm which can be closed with crimp caps.Vials with snap ring design with 11 mm rim diameter may be closed with PE snap-on caps or crimp caps.Conic vials are vials with tapered inner shape for small sample volumes, which can be closed with crimp caps or PE caps.Micro inserts are used to reduce the volume of standard sample vials for application with very small sample volumes. Vials are closed as usual. As an alternative for small volumes you may use the sample vials with conic inner shape (conic vials) mentioned above.Crimp caps can be used for crimp top vials and for vials with snap ring design. They are available with or without sealing disks, with centre hole, tear-off middle seal or as tear-off caps. Caps with centre hole are made from aluminium, aluminium with steel insert or steel. The two latter are magnetic.
Snap ring caps:
MACHEREY-NAGEL
Description Int. borediameter
Material / Sealing disk Thickness / Hardness PK Cat. No.mm mm / Image
Screw thread vials and screw capsVials with screwthreads are available in sizes of N 8, N 9 and N 13. They feature athreaded rim for screw caps.Conic vials with screwthreads and thick glass walls are micro reaction vials for small sample volumes, which are clo-sed with screw caps.Micro inserts are used to reduce the volume of standard sample vials for applications with very small sample volumes.Vials are closed as standard. As an alternative for small volumes you may use the sample vials with conic inner shape(conic vials) mentioned above.Screw caps for threaded vials available with or without sealing wads and with or without centre hole.Additionally we offer a versatile range of sealing wads for screw caps.
2MACHEREY-NAGEL
2
Description Capacity o.d. x height
PK Cat. No.
ml mmN 8-1, clear*N 8-1, amber*N 8-1, amber, label area with scale*Micro inserts for N 8-1, with mounted PP springMicro inserts for N8-1, standard, clearMicro inserts for N8-1, strongly taperedSprings for micro inserts 5 x 30 mmN9-1, clear, wide opening**N9-1, clear, wide opening, label area with scale**N9-1, amber, with label area, wide mouth**N 13-4 G, clearN 13-4 G, amberMicro inserts for vials N 13-4 GSprings for micro inserts 6 x 40 mm
*8-425 thread**short threadCombi packs of screw thread vials and screw caps N 8Sealing wads assembled MACHEREY-NAGELDescription PK Cat. No.
Combipack N 8-1, clear + N 8, with phenolic resin screw caps and red / natural sealsCombipack N 8-1, clear + N 8, with phenolic resin screw caps and red / white seals
10001000
9.003 4379.003 438
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14. ChromatographyVials/Ampoules, Septa, Caps
GENERAL CATALOGUE 2010/11
E & OE.
Screw caps N 8, N 9, N 13with or without sealing wadsN 8 8-425 threadsN 9 short threadsN 13 13-425 threads
1MACHEREY-NAGEL
1
Description Int. borediameter
Material / Sealing disk Thickness / Hardness PK Cat. No.mm mm / Image
N 8 Nat. rubber, red / colourless PTFEN 8 White silicone rubber / red PTFEN 8 White silicone rubber / blue PTFE /slit888
1.31.31.0603555
1001001007.060 4177.060 4197.085 892
Manual crimpers and opening pliersFor tightly closing crimp top vials manually, you need an appropriate crimper, foropening the corresponding opening pliers are used. If you have e. g. sample vials with20 mm rim diameter and the proper crimp caps (e. g. N 20 TB/oA), you can use crimpers N 20 and opening pliers N20 OE. The crimpers listed below can be adjusted to the respective rim height with the aid of a hexagonal key (except for the N8 crimper).
3MACHEREY-NAGEL
Description PK Cat. No.
8131120
1111
9.003 4709.003 4739.003 4719.003 475
3
Opening pliersFor sample, injection and infusion, crimp top bottles or vials with aluminium, or combination caps.
For dia. PK Cat. No.mm
111320
1111
9.003 5119.003 3679.003 3689.003 369
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14. ChromatographyVials/Ampoules, Septa, Caps
GENERAL CATALOGUE 2010/11
E & OE.
Containers for vialsavailable in 3 different sizes for small (N8 to N 11), medium size (N 13) and large (N 20) vials suited for freezers stackable MACHEREY-NAGEL
Description PK Cat. No.
81 positions, for vials N 8, N 9 and N 11, with integrated insert; size 130 x 130 x 45 mm, coded, with lid
49 positions, for vials N 13, with integrated insert; size 130 x 130 x 50 mm, with lid
25 positions, for vials N 20, with removable insert; size 130 x 130 x 80 mm, with lid
1
1
1
6.225 649
4.001 527
4.001 528
Sample vials, crimp topTransparent borosilicate glass vials.Choice of clear or amber.Supplied with and without labelling area, size 12 x 32 mm.Crimp top vials, standard neck.
1
Type PK Cat. No.
2.0 ml, transparent2.0 ml, transparent with labelling area2.0 ml, amber with labelling area100100100
0.10 ml conical, glass0.15 ml conical, glass with POLYSPRING0.25 ml flat bottom, glass
100100500
9.003 6039.003 6049.003 605
2 3 4
Sample vials, crimp topTransparent borosilicate glass vials. Choice of clear or amber.Supplied with and without labelling area, size 12 x 32 mm.Crimp top vials, wide mouth.
5 6
Type PK Cat. No.
2.0 ml, transparent2.0 ml, transparent with labelling area2.0 ml, amber with labelling area100100100
0.10 ml conical, glass0.15 ml conical,glass with Polyspring0.25 ml flat bottom, glass
100100500
9.003 6039.003 6049.003 605
3 4 5
Screwthread caps and septaDiameter 8 mm.Open screwthread cap.6 7
Type PK Cat. No.
Phenolic, blackPP, black 1000100 9.003 6339.003 634
6 7
SeptaSepta. 8 mm8Type PK Cat. No.
PTFEPTFE/RubberPTFE red/Silicone white1000100100
9.003 6359.003 6369.003 638
8
Three-layer septaLow density silicone wad sandwiched between two high density silicone layers. Working temperature max. 200°C.9
Hamilton
Dia. PK Cat. No.mm9.510.013.0
1212129.221 8039.221 8059.221 809
9
www.haeberle-lab.de
MACHEREY-NAGEL
FiltrationMACHEREY-NAGELFind out about high-quality products for filtration. As one of the leading filter paper manufacturers, we offer more than 7000 products from the following ranges:
• Quantitative (ashless) filter papers
• Qualitative filter papers
• Glass fibre filters
• Filter papers for technical applications
• Extraction thimbles
• PORAFIL® filter membranes
• CHROMAFIL® syringe filters
• CHROMABOND® MULTI 96 filters
• Special products and costum-made products
• ... and many more
Quality „Made in Germany“We produce our filter papers exclusively in Germany. Thereby we guarantee constant high quality for our customers.
With pride we look back to a almost 100-year-old successful history. Founded in 1911 MACHEREY-NAGEL today is a worldwide operating company. We develop and produce a large number of special products for analytical chemistry.
• Filter and filtration products
• Rapid tests for environmental, process and food analysis
• Test papers for medical urine analysis
• Consumables for chromatography
• Kits for purification of DNA and proteins
Be prepared for the futureRely on MACHEREY-NAGEL filter papers at any time in the future. As a family-owned company the friendly expert advice for our highly-valued customers as well as outstanding product quality have been the cornerstones of our corporate success for almost 100 years.
1178
14. ChromatographySyringes/Microlitre Syringes
GENERAL CATALOGUE 2010/11
E & OE.
Syringe TerminationsSyringe terminations are offered in a number of different configurationsdesigned to accommodate a broad range of applications. From cemented toremovable needles, and luer taper /TEFLON luer lock to special syringe fittings, syringe barrel termi-nations serve a key function at the interface of a syringe and its mating connection.N, NR (Cemented Needle, Cemented Needle Rheodyne)Needles are cemented into the glass syringe barrel at a point corresponding to the zero graduationmark. NR stands for syringes with Rheodyne specified needles.LTN (Luer Tip Cemented Needle)Needles are cemented into the conical glass luer tip of the barrel at a point corresponding to the zerograduation mark.SN (Special Needle)Hamilton offers customized syringes with special needles. State the following details: needle length,gauge, point style, and whether electro-tapered. Example of a correct specification: 701 SN, 70 mm,gauge 25, point style 3, not electrotapered. Without these details the corresponding standard syringe701 N will be supplied.RN (Removable Needle)Removable needles seat precisely to the zero graduation mark of the syringe. Allows the use of diffe-rent specification needles on the same syringe barrel. LT (Luer Tip)Ground glass syringe barrel with a male luer taper accepts most hypodermic needles. Use Kel-F hubneedles and connectors for a tight seal.KH (Knurled Hub)Removable needle, knurled hub is used on 7000 Series syringes, exclusively. The attachment of a spa-cer enables repeatable depth injections.SL (SampleLock)On/Off syringe valve with RN needle is cemented to a syringe barrel. Used for headspace, environ-mental sample collection and storage, pre-pressurization of gaseous samples for GC analysis, andsample spiking.TLL, TLLX (TEFLON Luer Lock)Male luer taper with nickel-plated brass hub accepts, and locks in place, luer hub needles and connec-tors. The X-style plunger stop incorporates a 6-32 UNC female thread on the end of the stop to allowattachment to drive arm mechanisms, such as on the MICROLAB 500 Series diluters/dispensers.C (Cheminert)1/4''-28 UNF, male fitting. Used in low volume applications where system dead volumes need to beminimized.AD (AccuDil®)M8 x 0.75, male fitting. Used with MICROLAB 1000 PLUS diluter/dispenser.DAD (Diluter AccuDil®)M8 x 0.75 male fitting with M6 x 1 side port. Used with MICROLAB 1000 PLUS diluter/dispenser.
1Hamilton1
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1179
14. ChromatographySyringes/Microlitre Syringes
GENERAL CATALOGUE 2010/11
E & OE.
Microlitre syringes, Needle TypesPoint Style 2:12° bevelled non-coring needle point recommended for septumpenetration. Ideal for gas chromatographic applications.Point Style 3:Blunt needle point (90°) for use with HPLC injection valves. Also recommended for applications whereexact dosing is required (e.g. thin layer chromatographs).Point Style 4:10-12° bevelled needle point recommended for life science applications; special point styles such as12°, and 45° are available on requestPoint Style 5:Conical needle with side port for penetration of septa, thin-gauged vinyls and plastics without coring;minimizes septum damagePoint Style AS:Special conical style needle point (8° taper) designed towithstand the demands of multiple injections; exclusively used onGC autosampler syringes
1Hamilton 1
Microlitre syringes, 700 seriesWith fixed needle (N, NR). NR stands for syringes with Rheodyne specified needles.Plungers are individually fitted, therefore cannot be interchanged and are not availableas replacement parts. Needles are electro-tapered.
Microlitre syringes, 700 series- 5 µl - 500 µl- For use with liquids- Cemented needles (N), removable needles (RN9, or luer tip (LT)- Plungers and Syringe barrels are not interchangeable or replaceable- Tight tolerances between the plunger and the barrel
Needles for RN syringesRemovable. Not for use in HPLC applications.1
Hamilton
Length 51 mmPoint style 2Type Ext.
dia.Int.dia.
Gauge Volume PK Cat. No.mm mm litres
7758-027758-037779-017779-03
0.50.70.70.7
0.130.150.410.15
26s22s22s22s
2.5 µl to 100 µl2.5 µl to 100 µl250 µl to 10 ml250 µl to 10 ml
6666
9.221 6929.221 6939.221 6949.221 695
1
Microlitre syringes, 800 seriesWith cemented-in, standard needles (N). With metal handle. Plungers are individuallyfitted, therefore cannot be interchanged and are not available as replacement parts.Needles are electro-tapered.
Microlitre syringes, 800 seriesType RN are equipped with metal plunger handles and removable needles. Type RN B/P are syringes only without metal plunger (replacement syringes).3
Microlitre syringes, 1700/1000 seriesGlass barrel with Teflon-coated plunger, seal and with Luer Tip (LT).Gas-tight microlitre syringe without needle. MS = Plunger stop fitted to prevent damage tothe Teflon seal. Prevents the plunger tip from reaching the end of the syringe.
Microlitre syringes, 1700/1000 seriesGlass barrel with Teflon-coated plunger and seal, and Luer lock outlet (TLL). TLLX with6-32 UNC female thread. Allows attachment to drive arm mechanisms, e.g. Microlab500 Series diluters/dispensers.Gas-tight microlitre syringe without needle. MS = Plunger stop fitted to prevent damage to the Teflon seal. Prevents the plunger tip from reaching the end of the syringe.
Needles for LT/TLL/TLLX syringesRemovable. With Luer tip made of Kel-F. All hypodermic needles available according tospecification in 3 mm to max. 1200 mm lengths.2
1800 Series Gastight Syringes10 µl - 250 µl- For use with gases and liquids- Removable needles (RN) or cemented needles (N)- Precision-machined Teflon PTFE-tipped plungers- Reinforced plungers- Plunger assemblies and glass barrels are replaceable
Microlitre syringes, 7000 series- 0.5 µL - 5 µL- For use with liquids- Removable needles (KH)- Positive displacement- Sample contained in the needle- No dead volume- Replaceable syringe parts
Spare unit for Microlitre syringes, 7000 seriesHamilton
Type PK Cat. No.
Spare unit for syringes 7000.5Spare unit for syringes 7001Spare unit for syringes 7002Spare unit for syringes 7105Spare unit for syringes 7101Spare unit for syringes 7102
Microlitre syringes, repeat dispensing deviceFor syringes 25 µl to 2.5 ml. Dispenses 2 % of total syringe volume each time theplunger is depressed.2
Hamilton
Type PK Cat. No.
PB 600-1 1 9.221 650
2
We can supply thismanufactorer’swholeproduct range !
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1184
14. ChromatographySample preparation/SPE
GENERAL CATALOGUE 2010/11
E & OE.
So
lid p
hase
extra
ction
Solid phase extraction (SPE) is a powerful method for sample preparation and is used by most chromatographers today.
More than 20 years ago MACHEREY-NAGEL designed and introduced CHROMABOND® SPE cartridges containing silica-based adsorbents. Since then we developed the widest range of phases and products for SPE based on silica and polymeric materials.
SPE has capabilities in a broad range of applications:
environmental analyses
pharmaceutical and biochemical analyses
organic chemistry
food analysis
Dri
nkin
g w
ate
rUrineFo
od
Soil
Vegetable
Fruit
Tabl
ets
Bloo
d
Was
te o
il
PesticidesPAHs PCBs
DrugsDyes
Vitamins
SPE is a form of digital (step-wise) chromatography designed to extract,
partition, and / or adsorb one or more components from a liquid phase
(sample) onto a stationary phase (adsorbent or resin). An adsorbed sub-
stance can be removed from the adsorbent by step-wise increase of elution
strength of the eluent (step gradient technique). SPE extends a chroma-
tographic system’s lifetime, improves qualitative and quantitative analy-
sis, and the demand placed on an analytical instrument is considerably
lessened.
In general, SPE is used for three important purposes in state-of-the-art analyses:
concentration of the analyte
(up to factor 10.000 - increase of chromatographic sensibility /
improved limits of detection)
removal of interfering compounds
(protection of subsequent analyses like HPLC, GC, TLC, UV or IR
spectroscopy, …)
changing an analyte’s environment to a simpler matrix more suit-
able for subsequent analyses
Advantages of SPE compared to classical liquid-liquid extrac-tion:
lower consumption of solvents
faster – enormous time savings
lower costs per sample
potential for automation
high consistency in individual sample handling
and different retention mechanisms
optimisation of extraction by variation or adjusting of the solid
Chromabondfi HR-Xhydrophobic polystyrene-divinylbenzene copolymerpH stability 1 to14high-purity material with highest reproducibility and lowest blank values due to a novel manufacturing processspherical particles 85µm; pore size 55 to 60Åvery high surface 1000m²/gcapacity 390mg/g (caffeine in water)excellent recovery rates especially for the enrichment of pharmaceuticals/active ingredients due to the spherical structure of the particles, very homogeneous surface, and optimised pore structurerecommended applications:pharmaceuticals/active ingredients from tablets, creams and water/waste waterdrugs and pharmaceuticals from urine, blood, serum and plasmatrace analysis of pesticides
Sample pretreatment: if necessary, adjust pH valueColumn conditioning: 5 ml methanolEquilibration: 5 ml waterSample application: slowly aspirate the sample through the columnColumn washing:Elution: after drying 3 x 2 ml methanol
Further analysis: if necessary, evaporate and redissolve in a suitable
Compounds:
A phenobarbital
B pentobarbital
C hexobarbital
Batch 1
0
50
100
A A AB B BC C C
Batch 2 Batch 3
Barbiturates from serum Column type:
CHROMABOND® HR-X 3 ml / 200 mg
Sample:Column conditioning: 5 ml methanol, 5 ml dist. waterSample application: 1 ml spiked serumColumn washing: 5 ml waterElution: after drying 3 x 2 ml methanol
Further analysis: ®18 ec, see MN Appl.
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1188
14. ChromatographySample preparation/SPE
GENERAL CATALOGUE 2010/11
E & OE.
Chromabondfi Easypolar modified polystyrene-divinylbenzene copolymer with a weak anionexchangerSpecific surface 650 to 700 m²/g, particle size 80µm, pore size 50 Å, pH stability 1 to 14. Due to bifunctional modifi-cation much more hydrophilic than conventional polystyrene-divinylbenzene polymers and thus easily wettable withwater.recommended applications:- polar herbicides/pesticides from water (acidic, neutral, basic)- polar phenols from water- polyaromatic compounds- polychlorinated biphenyls- drug analysis from urine, blood, serum, plasma pharmaceuticals/active ingredients from tablets, creamsDue to the bifunctional modification CHROMABONDfi Easy is considerably more hydrophilic than conventional polysty-rene-divinylbenzene polymers and thus easily wettable with water. The Easy effect: Aqueous samples can be loadeddirectly without preconditioning! This means that little or even no conditioning is needed, in contrast to standard SPEmaterials, where recovery rates normally decrease, in the worst case down to zero! Depending on the separation taskconditioning may be required and is recommended for method development. A positive side effect of the excellentwettability: there is no decrease of recovery rates, if the cartridge runs dry, therefore automation is easier or, in somecases compared to silica materials only feasible with CHROMABONDfi Easy, because a permanent vacuum can be usedwithout supervision.Further advantages of using a polymeric material:- high surface, this means very high binding capacity (2 5 times higher than silica-based adsorbents)- less adsorbent is needed in the cartridge (without losing sensitivity or recovery)- faster analysis, because the height of the adsorbent bed can be reduced- acidic or basic solvents (e. g. TFA) do not destroy the phase, or lead to unintended side productsBecause of the polar modification the material is suitable for a broad range of compounds (acidic, neutral, basic, polar and nonpolar substances). Highly reproducible recovery rates can be obtained, even if the cartridge runs dry (especially advantageous when using 96-well plates, where stopcocks are not available!)
Chromabondfi C18 ecoctadecyl modified silica phase for SPE, endcappedbase material silica, pore size 60Å, particle size 45µm for C18 ec, 100µm for C18 ec f (for fast flow),specific surface 500m²/g, pH stability 2 to 8octadecyl phases, endcapped, carbon content 14%very non-polar, hydrophobic interactions with a wide variety of organic compoundsadvantageous for clean-up of samples with large structural variations (polarity differences)recommended applications:non-polar compoundsaflatoxins, amphetamines, antibiotics, antiepileptics, barbiturates, caffeine, drugs, preservatives, fatty acids, nicotine, PAHs, pesticides, PCBs, heavy metals, vitaminsvery well suited for desalting of samples
CHROMABONDfi C18octadecyl modified silica phase for SPE, not endcappedbase material silica, pore size 60Å, particle size 45µm for C18, 100µm for C18 f (for fast flow),specific surface 500m²/g, pH stability 2 to 8 octadecyl phases, not endcapped, carbon content 14% similar to C18 ec,however possesses more free silanols (SiOH), which allow secondary interactions with polar groups of the analytesrecommended applications:non-polar compoundspesticides
Chromabondfi C18 Hydraoctadecyl modified silica phase for SPE of polar analytesbase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8special octadecyl phase for polar analytes, not endcapped, carbon content 15%recommended applications:more polar compounds like pesticides and their polar degradation products,phenols, phenoxycarboxylic acids,nitroaromatics, pharmaceuticals
octyl modified silica phase for SPE, not endcappedbase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8octyl phase, not endcapped, carbon content 8%similar to C18, however slightly more polarsecondary interactions with polar compounds are more pronounced due to shorter alkyl chainsrecommended applications:pesticides, PCBs
1MACHEREY-NAGEL
Capacity Capacity PK Cat. No.
ml mg13366
1002005005001000
10050503030
4.003 4606.203 6724.003 4614.003 4626.224 849
1
CHROMABONDfi C6H5phenyl modified silica phase for SPEbase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8phenyl phase, carbon content 8%polarity similar to C8in addition to hydrophobic interactions more selective adsorption is possible by Π-Π interactions due to the electrondensity of the phenyl ringrecommended applications:aflatoxinscaffeinephenols
2MACHEREY-NAGEL
Capacity Capacity PK Cat. No.
ml mg133
1002005001005050
4.003 4914.003 6086.224 848
2
Chromabondfi CNcyanopropyl modified silica phase for SPEbase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8cyanopropyl phase, carbon content 5.5%polar to mid-polarin addition to weak hydrophobic interactions selective interactions are possible due to the high electron density of theCN grouprecommended application:cyclosporinscarbohydrates
3MACHEREY-NAGEL
Capacity Capacity PK Cat. No.
ml mg133
1002005001005050
4.003 4734.003 6114.003 474
3
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14. ChromatographySample preparation/SPE
GENERAL CATALOGUE 2010/11
E & OE.
Chromabondfi NH2
aminopropyl modified silica phase for SPEbase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8aminopropyl phase, carbon content 3.5%polar, weak anion exchangerrecommended applications:trace elementslipids
Chromabondfi OHdiol modified silica phase for SPEBase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8diol phase, carbon content 5.5%polarproperties similar to SiOHrecommended application:antibioticsprostaglandins
2MACHEREY-NAGEL
Capacity Capacity PK Cat. No.
ml mg1336
100200500500
100505030
4.003 4704.003 6104.003 4716.224 847
2
Chromabondfi SiOHunmodified silica phase for SPEunmodified, weakly acidic silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8very polaradsorbs humidity from air, for this reason it should be kept well closed and if necessary dried before usedue to its high affinity for polar compounds it should not be conditioned with polar (e.g. methanol) or water-contai-ning solventsrecommended applications:aflatoxinschloramphenicolpesticidessteroidsvitamins
Chromabondfi Florisilfimagnesium silicate for SPEmatrix magnesium silicate (MgO to SiOH 15:85), high purity, particle size 150 to 250µmrecommended application:organic tin compounds,aliphatic carboxylic acids,PCBs, PAHs
Chromabondfi PApolyamide 6 for SPEmatrix polyamide 6, unmodified, high purity, particle size 40 to 80µmrecommended application:flavonoids, PAHs
1MACHEREY-NAGEL
Capacity Capacity PK Cat. No.
ml mg3366
2005005001000
50503030
4.003 5954.003 5117.089 0897.400 537
1
Chromabondfi PSApropylsulphonic acid modified silica cation exchanger for SPEBase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8propylsulphonic acid modified silicavery strong cation exchanger (capacity ~ 0.7meq/g)contrary to the SA phase no Π-Π interactionsrecommended applications:weak cations
2MACHEREY-NAGEL
Capacity Capacity PK Cat. No.
ml mg136
10050010001005030
4.003 6264.003 6276.206 233
2
Chromabondfi SAbenzenesulphonic acid modified silica cation exchanger for SPE (SCX)base material silica, pore size 60Å, particle size 45 µm, specific surface 500m²/g, pH stability 2 to 8 benzenesulphonic acid modified silicastrongly acidic cation exchanger (capacity ~ 0.5meq/g)adsorbent with hydrophobic and Π-Π interactions (benzene ring)ion exchange of organic compounds from aqueous matrixelution of interesting compounds with solvent systems, which compensate the ionic and nonpolar interactions, e.g. methanolic HClrecommended application:amino acidsamineschlorophyllPCBs
Chromabondfi SBquaternary ammonium modified silica anion exchanger for SPE (SAX)base material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8silica modified with quaternary aminestrongly basic anion exchanger (capacity ~ 0.3meq/g)not suited for very strong anions such as sulphonic acids, because these are difficult to eluterecommended application:organic acidscaffeinesaccharin
Polymer-based ion exchangers for SPEThe innovative cation exchanger offers:mixed-mode SPE phase with distinct cation exchange and RP properties excellent enrichment of basic analytesmodern support polymer with optimised pore structure and large specific surfacegood reproducibility, reliable and cost-efficient analysesmore aggressive washing procedures for matrix removal possiblecleaner samples and protection of analytical HPLC and GC instrumentationquantificationof analytes from heavily contaminated sampleslower limits of detection also for critical matrices... the perfect fit for basic analytes in comination with CHROMABOND® HR-X
2MACHEREY-NAGEL
2
Chromabondfi HR-XCbenzenesulphonic acid modified polymeric cation exchanger for SPEbase material spherical PS/DVB copolymer, pore size 65 - 75Å, particle size 85µm, very large specific surface800m²/g, pore volume 1.4cm³/g, pH stability 1 to 14 benzenesulphonic acid modification, exchange capacity1.0meq/g RP capacity 300mg/g (caffeine in water) high-purity material, highest reproducibility and lowest blank values due to an optimised production process outstanding recovery rates especially for the enrichment of basic analytesrecomended application: basic active ingredients from strongly matrix-contaminated samples e.g. urine, plasma,serum fungicides from food basic analytes, e.g. amines
3MACHEREY-NAGEL
Capacity Capacity PK Cat. No.
ml mg336
200500500303030
4.003 8214.003 8224.003 823
3
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1196
14. ChromatographySample preparation/SPE
GENERAL CATALOGUE 2010/11
E & OE.
Fractionation of acidic, neutral and basic analytes from serum
0
1.000
2.000
3.000
3.500
mAU
0
20
40
60
80
100
0 5 10 15 min
1
23
4
serum sample
without SPE
after SPE with HR-XC
after SPE with HR-XC
MN Appl. No. 304780
Column type: CHROMABOND® HR-XC / 3 ml / 200 mg
Sample: 3PO4
Column conditioning: 5 ml methanol, then 5 ml waterSample application:the columnWashing:Elution: fraction A: neutral and acidic an-alytes); then 5 ml methanol – NH3 fraction B: basic analytes)
Further analysis ® C18
® C8
Recovery rates [%]
Fraction A: neutral and acidic analytes
Fraction B: basic analytes
Compound HR-XC Compound HR-XC Oasis® Strata X-C
Suprofen 68
Naproxen 85 95 71 85
Tolmetin 73 3. Amitriptyline 94 78
Phenobarbital 4. Trimipramine 81
33
Hexobarbital
CHROMAFIXfi HR-XCColumns for HR-XC1
MACHEREY-NAGELSize Capacity PK Cat. No.
mgSML
155240500505050
4.003 8354.003 8364.003 837
1
www.haeberle-lab.de
1197
14. ChromatographySample preparation/SPE
GENERAL CATALOGUE 2010/11
E & OE.
PS-RP/PS-OH-/PS-H+/PS-Mix/PS-Ag+/PS-Ba2+phases for RP/ion chromatographybase material: high-purity polystyrene-divinylbenzene copolymers (PS/DVB)pore size 100 Ä, paricle size 100 µmverly low degree of swelling, thus very well suited for chromatographyreliable function over the whole pH range from 0-14different modifications for different applications from elimination of nonpolar compounds up to removal of specificpolar comonentsrecommended application:removal of interfering compounds- improves chromatographic separtions, if the interfering components overlap with the analyte in the chromatogram- improves lifetime of the chromatographic column, since interfering components can irreversibly block the columnpackingenrichment of the analytes
1MACHEREY-NAGEL
removal of organic interfering components from water PS-RP hydrophobic PS/DVB-copolymerPS-OH- strong PS/DVB anion exanger, OH- form capacity0.6 meq/gremoval or concentration of anions from water increasingthe pH value in acidic samples removal or concentrationof cations from water
decreasing the pH value in basic samples PS-H+ strong PS/DVB cation exchanger, H+ form, capaci-ty 2.9 meg/gdesalting of water PS-Mix mixture of PS-OH- and PS-H+removal of halide ions from water PS-AG+ strong PS/DVB cation exchanger, Ag+ formremoval of sulphate ions from water PS-Ba2+ strong PS/DVB cation exchanger, Ba2+ Form
Chromabondfi Drugspecial silica phase for SPE enrichment of drugs from urine or plasmabase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8special bifunctional modification - C8/SA (strong cation exchanger - benzenesulphonic acid)recommended application:enrichment of acidic, neutral and basic drugs from urine or plasma
1MACHEREY-NAGEL
Capacity Capacity PK Cat. No.
ml mg1336
100200500500
100505030
4.003 6966.802 7154.003 6994.003 697
1
Chromabondfi Drug IISpecial silica phase for SPE of THC and derivatives acidic analytes frombiological fluids (urine, blood ...)base material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8special bifunctional modification - C8/SB (strong anion exchanger - quaternary amine)recommended applications:extraction of THC and derivatives from urine, blood, serum, plasmaacidic analytes from biological fluids
2MACHEREY-NAGEL
Capacity Capacity PK Cat. No.
ml mg1336
100200500500
100505030
4.003 7004.003 6954.003 7014.003 698
2
Chromabondfi NH2/C18
Combination phase for SPE analysis of PAHs from water containing humic acidsSpecial combination phase:aminopropyl phase for removal of interfering humic acidsoctadecyl phase for enrichment of PAHsrecommended application:PAHs from water containing humic acids
3MACHEREY-NAGEL
Capacity Capacity PK Cat. No.
ml ml / mg66 500500 // 500 mg1000 mg 3030 6.228 257
4.003 675
3
PAHs from water containing humic acids
Column type: CHROMABOND® NHglass column
Sample pretreatment:
Column conditioning:
v/v)
Sample application:
Washing:
Elution: Cl
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14. ChromatographySample preparation/SPE
GENERAL CATALOGUE 2010/11
E & OE.
Chromabondfi CN/SiOHCombination phase for SPE analysis of PAHspecial combination phasecyanopropyl phase for selective adsorption of polycyclic aromatics via Π-Π interactionsunmodified silica phase for removal of polar compoundsrecommended applications:extraction of the 16 PAHs according to EPA from soil samples
1MACHEREY-NAGEL
Capacity Capacity PK Cat. No.
ml ml / mg366
500500500///
1000 mg1000 mg1000 mg5030250
4.003 5076.233 1284.003 514
BIGpacks: 4.003 514
1
Hydrocarbons from water
Column type: CHROMABOND® Na2SO4/Florisil®, 2000/2000 mg, 6 ml glass column
Internal standard solution: dissolve 20 mg n-tetracon-tane (C40H82) in petroleum ether, add 20 ml n-decane (C10H22
perparation of the extraction solution dilute standard
Sample pretreatment: adjust 900 ml water (10 °C) with HCl (12 mol/l) to pH 2 and add 80 g MgSO4
of the extraction solution, close the bottle and stir the
Column conditioning:Sample application: slowly aspirate or force the sample through the column Elution:the combined solutions from sample application and
Recovery rate: must be > 80 % for n
Chromabondfi Na2SO4/FlorisilfiCombination phase for SPE of hydrocarbons from water acc. to DIN H53/ISODIS 9377-4Special combination phase of sodium sulphate and Florisilfirecommended application:hydrocarbons from drinking, surface and waste waters
Soxhlet extractor. For low PAH contents (colourless or weakly coloured ex tracts) concentrate extract to 1/10 of its volume in a rotation evaporator.Column conditioning:
MN Appl. Nr. 301310
Sample application:
Washing:Elution:
NUCLEOSIL® 18 PAHrecovery rates see application 301310 at www.mn-net.com
Chromabondfi SA/SiOHCombination phase for SPE analysis of PCBsSpecial combination phase:SA: strongly acidic cation exchanger based on silica with benzenesulphonic acid modificationSiOH: unmodified silica for removal of polar compoundsrecommended application:extraction of PCBs from waste oil (hexane extract)
Column conditioning: 1 ml n-hexane Sample application:
force it into the adsorbent with 2 x 1 ml n-hexane
Elution: n-hexane through the column; collect all n-hexane fractions and if necessary adjust to a concentration suitable for subsequent analysis by either evaporting n-hexane in a stream of nitrogen or by dilution with n-hexaneRecovery rates:
Chromabondfi SiOH-H+/SACombination phase for SPE analysis of PCBsSiOH-H+: H2SO4-impregnated silica phase for oxidation of accompanying compounds to ionic and/or polar compoundsSA: strongly acidic cation exchanger based on silica with benzenesulphonic acid modification for removal of ionic andsulphur-containing compoundsrecommended application:extraction of PCBs from oil with reference to German industrial standard DIN 51527, part 1.This combination column is used together with a SiOH column.
1MACHEREY-NAGEL
Capacity Capacity PK Cat. No.
ml ml / mg33 50250 7.051 054
4.003 492BIGpacks: 4.003 492
1
Chromabondfi SiOH-H+/SAMACHEREY-NAGEL
Description PK Cat. No.
Kombi-Kit for extraction of PCBs from oil with reference to DIN 51527, part 1, 25 columns each CHROMABONDfi SiOH-H+/SA and CHROMABONDfi SiOH 25 7.051 055
PCBs in oil samples (German industrial standard DIN 51527)
Column type: CHROMABOND® SiOH-H2SO4/SA 3 ml, 500/500 mg and CHROMABOND® SiOH / 3 ml / 500 mgor Kombi-Kit PCB
Sample pretreatment: extract oil-contaminated solids with n-hexane. Homogenise other oil samples and dissolve 1.5 to 2.0 g in 50 ml n-hexane. Water which may cause turbidity can be removed with sodium sulphate.Column conditioning: let 1 ml nthe CHROMABOND® SiOH-H2SO4/SA column
MN Appl. No. 301380
Sample application: aspirate 500 µl sample through the CHROMABOND® SiOH-H2SO4/SA column. Place CHROMABOND® SiOH-H2SO4/SA column on top of SiOH
sample into the SiOH column with 2 x 1 ml n-hexane.Elution: elute SiOH column with 3 x 0.5 ml n-hexane; adjust to a suitable concentration for subsequent GC analysis by evaporation of n-hexane in a stream of nitrogen or by dilution with n-hexaneRecovery rates: PCB-28 99 %, PCB-52 95 %, PCB-101 99 %, PCB-138 94 %, PCB-153 99 %, PCB-180 96 %, PCB-209 101 %
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1201
14. ChromatographySample preparation/SPE
GENERAL CATALOGUE 2010/11
E & OE.
Chromabondfi NANSpecial phase for SPE analysis of PCB from sludgespecial combination phase:N: sodium sulphate for removal of trace waterA: SiOH/AgNO3 phase for removal of sulphur, sulphur-containing and polar compoundsrecommended application:extraction of PCB from sludge
1MACHEREY-NAGEL
Capacity Capacity PK Cat. No.ml ml / mg366
400700700///
140020002000///
4007007005030250
4.003 5036.204 6844.003 517
BIGpacks: 4.003 517
1
PCBs from sludge
Compounds investigated: polychlorinated biphenyls (PCB)This method is also suited for soil samples.
Sample pretreatment: extract 2 g lyophilised sludge with 70 ml nwith n-hexane
Column conditioning: n-hexane Sample application: aspirate 2 ml of the extract through the columnElution: n-hexane through the
5 ml with n-hexaneRecovery rates:
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1202
14. ChromatographySample preparation/SPE
GENERAL CATALOGUE 2010/11
E & OE.
Chromabondfi
In addition to the required adsorbent CHROMABONDfi Diamino MACHEREY-NAGEL offersa number of individually weighed and pre-mixed buffers and extraction mixtures,specially composed for different sample matrices.Procedure 1 for standard food samples: The sample is extracted with Mix II, then purified with Mix III or Mix IV (for food with higher fat content)Procedure 2 for complex or rich food samples: The sample is extracted with Mix I, then purified with- Mix III (samples with low fat content),- Mix IV (moderate content of chlorophyll and carotinoids; e. g. carrots, lettuce),- Mix V (high content of chlorophyll and carotinoids; e. g. bell peppers, spinach),- Mix VI (high fat content; e. g. avocados).
Mix I Citrat-Extraction-MixMix II Acetat-Extraction-MixMix III Diamino Clean-up MixMix IV Diamino/Carbon Clean-up MixMix V Diamino/Carbon Clean-up MixMix VI Diamino/C 18 ec Clean-up Mix
4g MgSO4, 1g NaCl, 0,5g Na2H-Citrat*1,5 H2O, 1g Na3-Citrat*2 H2O6 g MgSO4 - 1,5 g Na-Acetat0,9 g MgSO40,9 g MgSO4 - 0,015 g CHROMABOND Carbon0,9 g MgSO4 - 0,150 g CHROMABOND C 18 ec0,9 g MgSO4 - 0,045 g CHROMABOND Carbon
15 ml centrifuge tubes with screw capChromabondfi Diamino
Special silica phase for determination of pesticides in food samplesBase material silica, pore size 60Å, particle size 45µm, specific surface 500m²/g, pH stability 2 to 8Primary and Secondary Amine functions (PSA), 5% C removes polar compounds (e. g. organic acids, pigments,sugars) from matrices like fruit or vegetables similar phases: Supelclean PSA, Bond Elut PSArecommended application:special SPE phase for quick and cheap determination of pesticides in strongly matrix-contaminated samples by GCQuEChERS method = Quick Easy Cheap Effective Rugged SafeQuEChERS clean-up mixes containing 0.15 g Chromabondfi Diamino each
3MACHEREY-NAGEL
Capacity Capacity PK Cat. No.
ml mg36 200500 5030 4.003 652
4.003 653
3
SPE phases for food analysisQuEChERS method and pre-mixesWithin a fes years after its devleopment by Anastassiades et al. the QuEChERS method hasgained a leading position for determination of pesticide residues in food by GC-MS or LC-MS allowingrapid and cheap clean-up of stronglymatrix-contaminated samples.Standard clean-up of food samples10 g sample are homogenised with 10 ml acetonitrile. After adding the internal standard thesample is shaken with 4 g MgSO4 and 1 g NaCI and afterwards centrifuged.1 ml of the supernantant is spiked with 25 mg CHROMABOND® Diamino and 150 mg MgSO4 and shaken again. Aftercentrifugation the supernatant is injected into the GC/MS.For optimising the extraction of pH-dependent compounds, for minimising decomposition ofsensitive substances, and for broadending the matrix spectrum, different modifications of theQuEChERS method have been elaborated.
50 ml PP-centrifuge tube with crew cap 50 4.003 552
2
1203
14. ChromatographySample preparation/SPE
GENERAL CATALOGUE 2010/11
E & OE.
Chromabondfi vacuum manifolds and accessories for handling SPE columns and car-tridgesfor simultaneous preparation of up to 12, 16 or 24 samplesreplacement parts and accessories for special applicationsVacuum manifold complete consists of: glass cabinet with lid and lid gasket, removable needles on lower side of lid, vacuum gauge, control valve, valves and caps, variable rack.
3MACHEREY-NAGEL
3
Description PK Cat. No.
Vacuum manifold complete for up to 12 columns or cartridges (incl. reservoir tank)Vacuum manifold complete for up to 16 LV columnsVacuum manifold complete for up to 24 columns or cartridgesLids with gaskets for 12 columns (incl. Luer fittings and valves), plasticGaskets for lid, for 12 columnsLuer fittings for lid, femaleLuer fittings for lid, maleValves, plasticStainless steel needlesDrying attachment for 12 columnsProducts for protection from cross contamination valve, brass, tarnishedProducts for protection from cross contamination valve as aboveProducts for protection from cross contamination stainless steel connectorsProducts for protection from cross contamination PTFE connectors with valveTubing adaptor for 1,3 and 6 ml polypropylene columns (teflon, 1ml)
brass valves and stainless steel or PTFE connectors, the
has contact with the inert connector and not with the lid,
Drying attachment
If the eluate has to be evaporated, this can be performed
11
12), from
tions (13
SPE column with frits and solid phase
lid of vacuum manifold with bore
connector
stainless steel needle
www.haeberle-lab.de
1204
14. ChromatographySample preparation/SPE
GENERAL CATALOGUE 2010/11
E & OE.
CHROMABONDfi empty columns and accessories for SPEfor individual packing of SPE columns with CHROMABOND® adsorbents MACHEREY-NAGELDescription PK Cat. No.
Empty polypropylene column with PE filter elements, 3 mlEmpty polypropylene column with PE filter elements, 6 mlEmpty polypropylene column with PE filter elements, 15 mlEmpty polypropylene column with PE filter elements, 45 mlEmpty polypropylene column with PE filter elements, 30 mlEmpty polypropylene column with PE filter elements, 70mlEmpty polypropylene column with PE filter elements, 150 mlEmpty glass columns with glass fibre filter elements, 3 mlEmpty glass columns with glass fibre filter elements, 6 mlGlass fibre fliter elements for glass columns 3 mlGlass fibre fliter elements for glass columns 6 mlAdaptor (PP) for polypropylene columns (1,3 and 6 ml)Reservoir columns 70 ml, polypropylene, incl. 1 connector
Chromabondfi XTRFor liquid-liquid extractionBase material coarse-grained kieselguhr (also known as diatomaceous earth, hydromatrix, celite)large pore size, high pore volume, constantly high batch-to-batch qualitypH working range 1to13application:liquid-liquid extraction of highly viscous aqueous solutions such as physiological fluids (blood, plasma, and serum) in clinical chemistry, dyes in textiles, environmental and food analysis without use of a separation funnelhigh water loadability without water breakthrough during elution with organic solvents also suited for removing small amounts of water from solvents which are not miscible with water.advantages:fast, reproducible and economicalsimultaneous preparation of several samplesno problems with phase separation, no formation of emulsionshigh recovery ratessaving of time and solventsorganic solutions need not to be dried after separation
1 2 3MACHEREY-NAGEL
Capacity Capacity max. volume capacity of aq. solution
for 70 ml PP columns with 100 PE filter elementsfor NT20 with 50 PE filter elements (10 mm dia.)500 g1 kg5 kg
505050010005000
4.003 6566.900 7174.003 6584.003 6574.003 659
We can supply thismanufactorer’swholeproduct range !
www.haeberle-lab.de
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14. ChromatographySample preparation/SPE
GENERAL CATALOGUE 2010/11
E & OE.
Syringe CHROMAFIL®
®
121 °C
CHROMAFIL®
®
®
www.haeberle-lab.de
1207
14. ChromatographySample preparation/SPE
GENERAL CATALOGUE 2010/11
E & OE.
Syringe filters CHROMAFILfi
CHROMAFIL® Xtralabelled for method validation and cerificationXtra: imprint for direct identification of membrane type, diameter and pore sizeXtra: low bleeding polypropylene housingXtra: colour-free plain polypropylene
MACHEREY-NAGEL
Chromafilfi PET syringe filters with polyester membranehydrophilic multipurpose membraneFor polar as well as non-polar solventsthe HPLC filter, especially suited for mixtures of water and organic solventsfor TOC/DOC determinationnot cytotoxic, does not inhibit the growth of microorganisms and higher cellspolyester filter with integrated glass fibre prefilter (GF/PET): recommended for solutions with a high load of particulate matter or for highly viscous solutions
MS = minispike on filter exitCHROMAFILfi Xtra: 4.003 417/6.232 548/6.232 549BIG-BOX: 4.003 418/4.003 416/6.233 172/4.003 397/4.003 398/9.049 020/9.049 021
CHROMAFILfi RC syringe filters with membrane of regenerated cellulosehydrophilic membrane with very low adsorptionfor aqueous and organic/aqueous liquids i.e. polar and medium polar sample solutionsbinding capacity for proteins 84µg/filter
1MACHEREY-NAGEL
Type Poresize
Membranedia.
Housingcolourtop
Housingcolourbase
PK Cat. No.
µm mmRC-20/25RC-45/25RC-20/25RC-45/25RC-20/15 MSRC-20/15 MSRC-45/15 MSRC-45/15 MS
MS = minispike on filter exitBIG-BOX: 4.003 425/6.233 891/4.003 399/4.003 400
1
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1208
14. ChromatographySample preparation/SPE
GENERAL CATALOGUE 2010/11
E & OE.
Chromafilfi PTFE syringe filters with Teflonfi membranehydrophobic membranefor nonpolar liquids and gases very resistant towards all kinds of solvents as well as acids and bases flushing withalcohol, followed by water, makes the originally hydrophobic membrane more hydrophilic
1MACHEREY-NAGEL
Type Poresize
Membranedia.
Housingcolourtop
Housingcolourbase
PK Cat. No.
µm mmPTFE-20/25PTFE-45/25PTFE-20/25PTFE-45/25O-20/3O-45/3O-20/15 MSO-45/15 MSO-20/15 MSO-45/15 MS
MS = minispike on filter exitBIG-BOX: 4.003 410/9.049 060/4.003 394/4.003 395
1
Cellulose mixed esters (MV)hydrophilic membranefor aqueous or polar solutions
2MACHEREY-NAGEL
Type Poresize
Membranedia.
Housingcolourtop
PK Cat. No.
µm mmMV-20/25MV-45/25MV-20/25MV-45/25
0.200.450.200.45
25252525
labelledlabelledlabelledlabelled
100100400400
4.003 4074.003 4054.003 4084.003 406
BIG-BOX: 4.003 408/4.003 406
2
Cellulose acetate (CA)hydrophilic membranefor filtration of water-soluble oligomers and polymers, especially suited for biological macromoleculesvery high shape stability in aqueous solutionsextremely low binding capacity for proteins (21µg/filter)also available in a sterile package (S) for filtration under sterile conditions (each filter individually sealed)
3MACHEREY-NAGEL
Type Poresize
Membranedia.
Housingcolourtop
Housingcolourbase
PK Cat. No.
µm mmCA-20/25CA-45/25CA-20/25CA-45/25CA-20/25 SCA-45/25 S
hydrophilic membranefor aqueous and slightly organic liquids with higher flow ratesvery low adsorption for pharmaceuticals and proteins, withgood stability against acids and basesfor sterile filtration of non-sterile solutions we recommend the Chromafil Sterilizer PES (each filter individually sealed)binding capacity for proteins29µg/filter
2MACHEREY-NAGEL
Type Poresize
Membranedia.
PK Cat. No.µm mm
Sterilizer PES - blue rim 0.20 25 50 6.228 095
2
Polyvinylidene difluoride (PVDF)hydrophilic membranefor polar and nonpolar solutions, water-soluble oligomers and polymers like proteinsbinding capacity for proteins 82µg/filterThe PVDF filter with integrated glass fibre prefilter is recommended for filtration of biological samples with high parti-cle loads. This filter features a high binding capacity for proteins.also suited for filtration of polar and non-polar solutions
Glass fibre (GF)inert filter, nominal pore size 1µm, allows higher flow rates than smaller pore filtersfor solutions with high loads of particulate matter or for highly viscous solutions (e. g. soil samples, fermentation broths)as prefilters for other Chromafil filters, they prevent plugging of the membrane.
1MACHEREY-NAGEL
1
Type Poresize
Membranedia.
Housingcolourtop
Housingcolourbase
PK Cat. No.
µm mmGF- 100/25GF- 100/25GF- 100/15 MS
nom. 1.0nom. 1.0nom. 1.0252515
labelledlabelledblue natural100400100
6.232 3624.003 4239.049 077
MS = minispike on filter exitCHROMAFILfi Xtra: 6.232 362/4.003 423BIG-BOX: 4.003 423
Chromabondfi Multi 96 filter plates96-well polypropylene plates for simultaneous filtration of 96 samplesadvantages of this high-throughput system are:- economical by saving time and solvent- use of multi-channel pipettors facilitates liquid transfer steps- readily adaptable to all common automated/robotic handling systems- minimised dead volume (≤ 40µl)- membrane materials correspond to the respective Chromafilfi syringe filters
2MACHEREY-NAGEL
2
Description PK Cat. No.
Filter plates with cellulose mixed ester filter elements (0.20 µm)Filter plates with cellulose mixed ester filter elements (0.45 µm)Filter plates with cellulose mixed ester filter elements (3.0 µm)Filter plates with RC filter elements (regenerated cellulose, 0.2 µm)Filter plates with RC filter elements (regenerated cellulose, 0.45 µm)Filter plates with PTFE filter elements (0.2 µm)Filter plates with PTFE filter elements (0.45 µm)Filter plates with PTFE filter elements (1.0 µm)Filter plates with PTFE filter elements (3.0 µm)Filter plates with PE filter elements (20 µm)Filter plates with PE filter elements (50 µm)Filter plates with glass fibre filter elements (nominal 1 µm)Filter plates with glass fibre filter elements (nominal 3 µm)Vacuum manifold for monoblocks, with reservoir tank, vacuum gauge and control valve, required for filtration with 96-well filter plates
The following table lists the chemical compatibility of our CHROMAFIL® materials. The chemical compatibility depends on several parameters such as time, pressure, temperature and concentration.
In most cases, CHROMAFIL®
of limited compatibility.
although PP shows only limited resistance towards THF.
Solvent Material CA RC PA PTFE PES PET GF PP
Acetaldehyde
Acetic acid, 100 %AcetoneAcetonitrile
Benzene
n–Butanol
Cyclohexane
Ethanol
Ethyl acetate
Ethylene glycol
Formic acid, 100 %
Hydrochloric acid, 30 %Methanol
Oxalic acid, 10 % aqueousPetroleum ether
Phosphoric acid, 80 %
Potassium hydroxide, 1 mol/l
2–Propanol
Sodium hydroxide, 1 mol/l
Tetrachloromethane
Tetrahydrofuran
Toluene
Trichloroethene
Trichloromethane
Water
Xylene
resistant, not resistant, limited resistance
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14. ChromatographyChromatography columns/HPLC
GENERAL CATALOGUE 2010/11
E & OE.
Colu
mns fo
r HPLC
NUCLEODUR® is a fully synthetical type B silica (silica of 3rd
advanced physical properties like totally spherical particle shape, outstanding surface microstructure, high pressure stability and low metal content.
NUCLEODUR® as a state-of-the-art silica is the ideal base material for modern HPLC
pioneering research in chromatography for
NUCLEOSIL® silica.
ca itself. Shortcomings in the surface geometry of the particles or metal contaminants are the main reasons for inadequate coverage with the covalently bonded alkylsilanes in the subsequent derivatization steps. It is well known, that poor surface coverage and, in con-sequence, high activity of residual free silanols often results in peak tailing or adsorption, particularly with basic compounds.
Particle shape and surface symmetry
NUCLEODUR® silicas are synthesized in a unique and carefully controlled manufacturing process which pro-vides silica particles, which are totally spherical. The picture shows the outstanding smoothness of the NUCLEODUR® surface.
Purity
As already mentioned above, a highly pure silica is re-quired for achieving symmetric peak shapes and maxi-mum resolution. Inclusions of e. g. iron or alkaline earth metal ions on the silica surface are largely responsible for the unwanted interactions with ionizable analytes, e. g. amines or phenolic compounds.
NUCLEODUR® is virtually free of metal impurities and low acidic surface silanols. Elemental analysis data of NUCLEODUR®
Elementary analysis (metal ions) of NUCLEODUR® 100-5
Aluminium < 5 ppm
Iron < 5 ppm
Sodium < 5 ppm
Calcium < 10 ppm
Titanium < 1 ppm
Zirconium < 1 ppm
Arsenic < 0.5 ppm
Mercury < 0.05 ppm
Pressure stability
The totally spherical and 100% synthetic silica gel ex-hibits an outstanding mechanical stability, even at
rates.
In addition, after several cycles of repeated packing, no
ter is of prime importance for preparative and process-scale applications.
Physical properties of NUCLEODUR®
Surface (BET) 340 m2/g
Pore size 110 Å
Pore volume 0.9 ml/g
NUCLEODUR®
on NUCLEODUR® silica have been developed over the last years providing a full range of
every separation:
NUCLEODUR® C18 Gravity and C8 Gravity
NUCLEODUR® C18 Isis
NUCLEODUR® C18 Pyramid
NUCLEODUR® Sphinx RP
NUCLEODUR® CN and CN-RP
NUCLEODUR® NH2 and NH2-RP
NUCLEODUR® C18 ec and C8 ec
For important properties of NUCLEODUR® phases please see our summary.
NUCLEODUR® high purity silica for HPLC
www.haeberle-lab.de
Colu
mns fo
r HPLC
Phase
A B C
C18 Gravity
, high
L1
NU
CLE
OD
UR
®
(Si-O
2) n
C8 Gravity
L7
NU
CLE
OD
UR
®
(Si-O
2) n
C18 Isis
L1 NU
CLE
OD
UR
®
(Si-O
2) n
C18 Pyramid
C18
L1
NU
CLE
OD
UR
®
(Si-O
2) n OH
OH
Sphinx RP 18
L1 L11 NU
CLE
OD
UR
®
(Si-O
2) n
C18 ec
L1
NU
CLE
OD
UR
®
(Si-O
2) n
Si OSi(CH3)3
Si OH
C8 ec
L7
NU
CLE
OD
UR
®
(Si-O
2) n
Si OSi(CH3)3
Si OH
CN /
L10
NU
CLE
OD
UR
®
(Si-O
2) n
Si OSi(CH3)3
Si OH
C N
C N
NH2 / NH2 L8
NU
CLE
OD
UR
®
(Si-O
2) n Si
Si
OH
OH
NH2
NH2
SiOH L3 (Si-O2)n Si OH
Overview of NUCLEODUR® HPLC phases
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14. ChromatographyChromatography columns/HPLC
GENERAL CATALOGUE 2010/11
E & OE.
www.haeberle-lab.de
1214
14. ChromatographyChromatography columns/HPLC
GENERAL CATALOGUE 2010/11
E & OE.
Columns for H
PLC
NUCLEOSIL® C18 HD®
18 18;® C18 (2),
® ®
®
only hydrophobic
interactions
O
NH3C
CH3Si(CH3)3
18 NUCLEOSIL® C8 HD®
8 8;® C8;
®
NUCLEOSIL® C18 AB®
®
steric interactions
hydrophobic
interactions
®; ® AQ;
®
hydrophobic
interactions
polar interactions
O
OH
N
H3C
CH3
hydrophobic
interactions NO2
18 phase
NUCLEOSIL® C18 ® ®
® C18; ® ®
®
only hydrophobic
interactions
O
NH3C
CH3Si(CH3)3
SiOH
8
NUCLEOSIL® C8 ec / C8® ®
® C8; ® ®
NUCLEOSIL®
,
polar interactions
hydrophobic
interactions
O
HOC N
C N
NUCLEOSIL®
NH2 / NH2
polar / ionic
interactions,
hydrophobic
interactions
NH3
+
OH
O
® polar / ionic
interactionsO2NSiOH
An optimised phase for every separations
www.haeberle-lab.de
Colu
mns fo
r HPLC
decrease of analysis time (ultra fast HPLC)
suitable for LC/MS due to low bleeding characteristics
all NUCLEODUR®
C
NUCLEODUR®
®
NUCLEODUR® C Competitor A
–
Column: 50 x 4 mm NUCLEODUR® C18 GravityA) 3 µm, B) 1.8 µm
Eluent: acetonitrile – water (80:20, v/v)
Flow rate: 2 ml/min
Pressure: A) 80 bar, B) 160 bar
Detection: UV, 254 nm
Peaks:
1. Naphthalene2. Ethylbenzene
0.4 0.6
A)
3 µm
Rs = 1.11
1
2
0.4 0.6 min
B)
1.8 µm
Rs = 1.42
1
2
min0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0
1.8 µm, 3.00 ml/min (320 bar)
1.8 µm, 2.00 ml/min (220 bar)
1.8 µm, 1.15 ml/min (125 bar)
o-Terphenyl
m-Terphenyl
p-Terphenyl
Triphenylene
5 µm, 0.8 ml min (50 bar)
Column: 50 x 4 mm (for 5 µm
125 x 4 mm) NUCLEODUR®
C18 IsisEluent: 100 % methanol
Flow rates and pressure see
Detection: UV, 254 nm
1215
14. ChromatographyChromatography columns/HPLC
GENERAL CATALOGUE 2010/11
E & OE.
www.haeberle-lab.de
1216
14. ChromatographyChromatography columns/HPLC
GENERAL CATALOGUE 2010/11
E & OE.
HPLC columns with NUCLEODURfi PhasenNUCLEODUR® C18 - C 8 Gravity nonpolar high density phasesavailable as octadecyl (C18 -USP L!) and octyl (C8 - USP L7) modificationsPore size 110 Ä; particle sizes 1.8 µm, 3 µm and 5 µm for C18, 1.8 and 5 µm for C87, 10, 12 and 16 µm particles for preparative separations on requestcarbon content 18 % C for C18 , 11 % C for C8ideal for method developmentallows HPLC at pH extremes (pH 1 - 11)suitable for LC/MS due to low bleeding characteristicsrecommended for overall sophisticated analytical separationscompound classes separated so far: pharmaceuticals, e.g. analgesics, antiinflammatory drugs, antidepressants; herbicides; phytopharmaceuticals; immunosppressants
MACHEREY-NAGEL
Nucleodurfi C18 Gravity, 1.8µmEC analytical columnsparticle size 1.8µm, 18% C
MACHEREY-NAGEL
Type Length PK Cat. No.
mm2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.
Nucleodurfi C18 Gravity, 3µmEC analytical columnsparticle size 3µm, 18% C
MACHEREY-NAGEL
Type Length PK Cat. No.
mm2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.
Guard columns for Nucleodurfi C18 Gravity, 3µmMACHEREY-NAGEL
Type PK Cat. No.
2 and 3 mm i.d.4 and 4.6 mm i.d.
33
4.004 6244.004 625
www.haeberle-lab.de
1217
14. ChromatographyChromatography columns/HPLC
GENERAL CATALOGUE 2010/11
E & OE.
Nucleodurfi C18 Gravity, 5µmEC analytical columnsparticle size 5µm, 18% C
MACHEREY-NAGEL
Type Length PK Cat. No.
mm2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.
mm2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.
mm2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.
Guard columns for Nucleodurfi C18 Isis, 3µmMACHEREY-NAGEL
Type PK Cat. No.
2 and 3 mm i.d.4 and 4.6 mm i.d.
33
4.004 6294.004 630
Nucleodurfi C18 Isis, 5µmEC analytical columnsparticle size 5µm, 20% C
MACHEREY-NAGEL
Type Length PK Cat. No.
mm2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.
VarioPrep columns for preparative HPLC C18 Isis, 3, 5µmMACHEREY-NAGEL
Type PK Cat. No.
8 mm i.d.16 mm i.d.
22
4.004 8024.004 803
10 x 8mm ID VarioPrep guard columns require the VP 8mm guard column holder and are suited for 8 and 10mm ID VP columns,20 x 16mm ID VarioPrep guard columns require the VP guard column holder 16mm and are used for 16 and 21mm ID VP columns.
NUCLEODUR C18 Pyramid phase for highly aqueous eluentsstabel in 100 % aqueous eluent systems- SUP L1pore size 110 Ä, particle sizes 1.8 µm, 3 µm and 5 µm; 14 % C7 and 10 µm particles for preparative separations on requestinteresting polar selectivity featuresexcellent base deactivation; suitable for LC/MS due low bleeding characteristicspH stability 1 - 9classes of compounds separated so far: analgesics, penicillin antibiotics, nucleic acid bases, water-soluble vitamins, complexing agetns, organic acids
MACHEREY-NAGEL
RP HPLC with highly aqueous eluents
Stability features
® C18
® C18
®
C18
Retention characteristics
tional C18
Stability test
initial injection
0
1
2
3
1
2
1
2
3
3
5 min 0 5 min
Pyramid
18
stop
stop
2 4
1 µl Peaks:
Separation of very polar compounds ® C18
4
Peaks:
1
2
20 min
t0
Length
mm1020
www.haeberle-lab.de
1222
14. ChromatographyChromatography columns/HPLC
GENERAL CATALOGUE 2010/11
E & OE.
Nucleodurfi C18 Pyramid, 1.8µmEC analytical columnsparticle size 1.8µm, 14% C
MACHEREY-NAGEL
Type Length PK Cat. No.
mm2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.
Nucleodurfi C18 Pyramid, 3µmEC analytical columnsparticle size 3µm, 14% C
MACHEREY-NAGEL
Type Length PK Cat. No.
mm2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.
Guard columns for Nucleodurfi C18 Pyramid, 3µmMACHEREY-NAGEL
Type PK Cat. No.
2 and 3 mm i.d.4 and 4,6 mm i.d.
33
4.004 7394.004 740
Nucleodurfi C18 Pyramid, 5µmEC analytical columnsparticle size 5µm, 14% C
MACHEREY-NAGEL
Type Length PK Cat. No.
mm2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.
VarioPrep columns for preparative HPLC C18 Pyramid, 3, 5µmMACHEREY-NAGEL
Type PK Cat. No.
8 mm i.d.16 mm i.d.
22
4.004 7884.004 789
VarioPrep guard columns require the 8mm VP guard column holder and are suited for 8 and 10mm ID VP columns,20 x 16mm ID VarioPrep guard columns require the 16mm VP guard column holder and are used for 16 and 21mm ID VP columns.
NUCLEODURfi Sphinx RP bifunctional RP phasedistinct selectivity based on bifunctional surface coverage - USP L1 and USP L11pore size 110 Ä, particle sizes 1.8 µm, 3 µm and 5 µm; 14 % Chigh density of covalently bonded silanes for tailing-free peaks widens the scope for method developmentpH stability 1 - 10suitable for LC/MS due low bleeding characteristicshigh reproducibility and consistent quality due to tight QC proceduresrange of application: quinolone antibiotics, sulfonamides, xanthines, substituted aromatics
MACHEREY-NAGEL
Length
mm1020
www.haeberle-lab.de
1224
14. ChromatographyChromatography columns/HPLC
GENERAL CATALOGUE 2010/11
E & OE.
Alternative RP selectivity
®
selectivity features generated by a well-balanced ratio of
bination of classical hydrophobic with interactions
mm2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.
Guard columns for Nucleodurfi C18 SPHINX RP, 3µmMACHEREY-NAGEL
Type PK Cat. No.
2 and 3 mm i.d.4 and 4.6 mm i.d.
33
4.004 6964.004 697
Nucleodurfi, Sphinx RP, 5µmEC analytical columnsparticle size 5µm, 14% C
MACHEREY-NAGEL
Type Length PK Cat. No.
mm2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.2 mm i.d.3 mm i.d.4 mm i.d.4.6 mm i.d.
VarioPrep columns for preparative HPLC SPHINX RP, 3, 5µmMACHEREY-NAGEL
Type PK Cat. No.
8 mm i.d.16 mm i.d.
22
4.004 7954.004 796
10 x 8mm ID VarioPrep guard columns require the 8mm VP guard column holder and are suited for 8 and 10mm ID VP columns,20 x 16mm ID VarioPrep guard columns require the 16mm VP guard column holder and are used for 16 and 21mm ID VP columns.
www.haeberle-lab.de
1226
14. ChromatographyChromatography columns/HPLC
GENERAL CATALOGUE 2010/11
E & OE.
NUCLEODURfi C18 ec - C8 ec nonpolar phases for routine analysesavailable with medium density octadecyl (C18 - USP L1) and octyl (C8 - USP L7)modificationpore size 110 Ä, particle sizes, 3 µm and 5 µm;7 µm, 10 µm, 12 µm, 16 µm, 20 µm, 30 µm und 50 µm for preparative separations on requestfor daily routine analysis and up-scaling for preparative HPLCpH stability 1 - 9carbon content 17.5 % C for C18 , 10.5 % C for C8high reproducibility from lot to lotfor standard routine applications in reversed phase chromatography
VarioPrep columns for preparative HPLC 100-5, 100-7, 100-10, C18 ecMACHEREY-NAGEL
Type PK Cat. No.
8 mm i.d.16 mm i.d.
22
4.004 7694.004 770
10 x 8mm ID VarioPrep guard columns require the 8mm VP guard column holder and are suited for 8 and 10mm ID VP columns,20 x 16mm ID VarioPrep guard columns require the 16mm VP guard column holder and are used for 16 and 21mm ID VP columns.
VarioPrep columns for preparative HPLC 100-5 C8 ecMACHEREY-NAGEL
Type PK Cat. No.
10 mm i.d.21 mm i.d.
22
4.004 7714.004 772
10 x 8mm ID VarioPrep guard columns require the 8mm VP guard column holder and are suited for 8 and 10mm ID VP columns,20 x 16mm ID VarioPrep guard columns require the 16mm VP guard column holder and are used for 16 and 21mm ID VP columns.
NUCLEODURfi CN/CN-RP cyano-modified high purity silica phasepore size 110 Ä, particle sizes 3 µm and 5 µm; 7 % C - USP L10multi-mode columns (RP and NP)widens the scope in selectivitydiffernet retention characteristics compared to C8 and C18stable against hydrolysis at low pH values, working range pH 1-8high reproducibility from lot to lotclasses of compounds separated so far: tricyclic antidepressants, steroids, organic acids
MACHEREY-NAGEL
®
A) 250 x 4 mm NUCLEODUR® 100-5 C18B) 250 x 4 mm NUCLEODUR® 100-5 CN-RP
Guard columns for Nucleodurfi 100-5 CNMACHEREY-NAGEL
Type PK Cat. No.
4 and 4.6 mm i.d. 3 4.004 654As guard columns for EC columns use ChromCartfi guard column cartridges with guard column adaptor EC.
NUCLEODURfi NH2 /NH2 -RP amino-modified high purity silica phasepore size 110 Ä, particle sizes 5 µm; 2.5 % C; not endcapped - USP L8multi-mode columns (RP and NP)normal phase chromatography (NP) with hexan, dichloromethane or 2-propanol as mobile phase for polar com-pounds such as substituted anilines, esters, chlorinated pesticidesreversed phase chromatography (RP) of polar compounds like sugars in aqueous-organic eluent systemsion exchange chromatography of anions and organic acids using common buffers and organic modifiersstable against hydrolysis at low pH, working range pH 2-8, 100 % stable in water, suitable for LC-MSclasses of compounds separated so far:polar compounds under RP conditions (sugars, DNA bases), hydrocarbons under NP conditionsEluent in column is n-heptane for the NP mode - RP columns are delivered in acetonitrile - water. For changing the solvent system a rinsing step with THF may be necessary.
NUCLEOSILfi 100-5 C18 PAH special octadecyl phase for PAH analysisbase material NUCLEOSILfi silica, particle size 5 µm, pore size 110 Ä; polymeric coating- USP L1eluent in column acetonitrile/water 70:30allows efficient gradient separation of the 16 PAH in accordance with EPAdetection of the separated PAH by UV (250 to 280 nm), with diode array or with fluorescence detection at differentwavelegths for excitation and emission (acenaphthylene cannot be analysed with fluorescence detection)
MACHEREY-NAGEL
Rapid separation of 16 PAH according to EPA® 100-5 C
Guard columns for NUCLEOSILfi 100-5 C18 PAHMACHEREY-NAGEL
As guard columns for EC columns use ChromCartfi guard column cartridges with guard column adaptor EC.HPLC columns for enantiomer separation
NUCLEOCEL ALPHA enantiomer separation based on amylose derivativesbase material silica,chiral selector amylose-(3.5-dimethylphenylcarbamate) - USP L51similar phases: Chiralpakfi AD, Kromasilfi, AmyCoatfi, Europak 01high resolution type (S) with 5 µm particle size, allows use of shorter columns (150 mm) for faster separations, pres-sure stability up to ~ 150 bar(2000 psi)NUCLEOCEL ALPHA for normal phase applications:eluent in column n-heptane - propanol-2 (90:10, v/v)typical eluents are heptane - propanol mixturesNUCLEOCEL ALPHA-RP for reversed phase applications:eluent in column acetonitrile - water (50:50), v/v)designed for use either in polar organic mode or with eluents containing high concentrations of chaotropic salts suchas perchloraterecommended application: pharmaceutically active compounds, chiral pollutants (e. g. herbicides, PCB), chiral compounds in food (dyes, preservatives), chiral catalysts and bioorganic compounds
Guard columns for Nucleocel ALPHA-RP SMACHEREY-NAGEL
Type PK Cat. No.
4.6 mm i.d. 1 4.002 510As guard columns for EC columns use ChromCartfi guard column cartridges with guard column adaptor EC. 8mm ChromChartfi guard column cartridges inpacks of 3, all other columns in packs of 1.
HPLC columns for enantiomer separationNUCLEOCEL DELTA enantiomer separation based on cellulose derivativesbase material silica,chiral selector Cellulosetris-(3.5-dimethylphenylcarbamate) - USP L40similar phases: Chiralcelfi OD, Kromasilfi, CelluCoat™, Eurocelfi 01standard particle size 10 µm,high resolution type (S) with 5 µm particle size, allows use of shorter columns (150 mm) for faster separations, pres-sure stability up to ~ 150 bar (2000 psi)NUCLEOCEL DELTA for normal phase applications:eluent in column n-heptane - propanol-2 (90:10, v/v)typical eluents are heptane - propanol mixturesNUCLEOCEL DELTA-RP for reversed phase applications:eluent in column acetonitrile - water (40:60), v/v)designed for use either in polar organic mode or with eluents containing high concentrations of chaotropic salts suchas perchloraterecommended applications: pharmaceutically active compounds, chiral pollutants (e. g. herbicides, PCB), chiralcompounds in food (dyes, preservatives), chiral catalysts and bioorganic compounds
Guard columns for Nucleocel DELTA-RPMACHEREY-NAGEL
Type PK Cat. No.
4.6 mm i.d. 1 4.002 514
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1236
14. ChromatographyChromatography columns/HPLC
GENERAL CATALOGUE 2010/11
E & OE.
®
Injection volume: 5 µl
2 4
Temperature: 35 °C
Citric acid
Tartaric acid
Pyruvic acid
Lactic acid
Propionic acid
i
0 5 10 15 20 25
®
Cellobiose
Trehalose
Lactose
Palatinose
Lactulose
Xylose
Lactitol
Palatitol
meso
Xylitol
0 5 10 15 20
Nucleogelfi SUGAR 810 HValco type columnsseparation of sugars, sugar alcohols and organic acids - USP L17eluent in column 0.01N H2SO4
MACHEREY-NAGEL
Type Length PK Cat. No.
mm7.8 mm i.d. 300 1 4.002 276
Guard columns for Nucleogelfi SUGAR 810 HMACHEREY-NAGEL
Type PK Cat. No.
7.8 mm i.d. 2 4.002 277
NUCLEOGEL® SUGAR 810 separation of sugarssulphonated polystyrene/divinylbenzene resins in diffent ionic formsand RP chromatographyseparation mechanism includes ion exclusion, ion exchange, size exclusion, ligand exchange as well as NP and RP chromatographyH+ form: separation of sugars, sugar alcohols and organic acids - USP L17 - eluent in column 0.01 N H2SO4Ca2+ form: separation of mono-, di- and oligosaccharides - USP L19eluent in column water
MACHEREY-NAGEL
www.haeberle-lab.de
Nucleogelfi SUGAR 810 CaValco type columnsseparation of mono-, di- and oliogosaccharides - USP L19eluent in column water
MACHEREY-NAGEL
Type Length PK Cat. No.
mm7.8 mm i.d. 300 1 4.002 274
1237
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GENERAL CATALOGUE 2010/11
E & OE.
Guard columns for NUCLEOGEL fi SUGAR 810 CaMACHEREY-NAGEL
Type PK Cat. No.
7.8 mm i.d. 2 4.002 275ChromCart Nucleogelfi SUGAR 810 guard column cartridges measure 30 x 4mm and require the 30mm CC column holder, (columns in packs of 1, guard columns in packs of 2
MN column systemsVarioPrep columnscolumn system for preparative HPLC manufactured from stainless steel with one adjustable end fitting (on request, columns with two adjustable end fittingsare also available, e.g for frequent use of backflushing techniques)allows compensation of a dead volume, which could result at the column inlet after some time of operation, without need for opening the columnpacked with NUCLEODURfi and NUCLEOSILfi spherical silica
MACHEREY-NAGEL
Available standard dimensions of
[mm]
Length [mm]
10* 20* 50 100 125 150 250 500
8 x x x x x10 x x x x x16 x x x x x21 x x x x x32 x x x40 x x x x x x50 x x x80 x x x
VarioPrep guard column holders and replacement partsO-rings available on request.1
MACHEREY-NAGEL1
Description PK Cat. No.
for 8 and 10 mm ID guard columnsfor 16 and 21 mm ID guard columns 11 4.002 1764.002 175
Accessories for VarioPrep2MACHEREY-NAGEL
Description PK Cat. No.
VP plunger fitting 10 mmVP nut 10 mmVP sealing element set 10 mmVP sealing ring set 10 mmVP MN Inert sealing combination 10 mmVP plunger fitting 21 mmVP nut 21 mmVP sealing element set 21 mmVP sealing ring set 21 mmVP MN Inert sealing combination 21 mm
MN column systemsEC standard columns for analytical HPLCanalytical column system manufactured from stainless steel M 8 outer threads on both endscombination of sealing element and very fine-meshed stainless steel screen, PTFE sealing ring and fitting adaptor column heads SW 12 with inner threads M8 x 0.75 and UNF 10-32as built -in guard columns use ChromCartfi guard column cartridges with 8 mm lenght with the guard column adaptor ECpacked with NUCLEODURfi spherical silica
MACHEREY-NAGEL
[mm] 8* 20 50 75 100 125 150 200 250
2 – x x x x x x x x x x
x x x x x x x x x x x
4 x x x x x x x x x x x
– x x x x x x x x x x
Accessories and replacement parts for EC columnsMACHEREY-NAGEL
Description PK Cat. No.
Guard column adapter EC1/16" nut fpr connecting 1/16" capillaries1/16" ferrule1/16" end cap, plasticEC fitting adaptorEC column head (nut)EC PTFE sealing ringsealing-set combination for EC columns
Chromabondfi Flash cartridges for Biotagefi systemsproduct range designed for use in Flash systems of Biotage AB (Flash 12i™ andFlashMaster™) without additional connectors or capillarieson request all column types listed below can be packed with any Chromabondfi adsorbent (please note that other packings often result in differing adsorbent weights)Chromabondfi Flash RS columns for Teledyne Iscofi systems (RS) on request.
1MACHEREY-NAGEL
1
Description Columnlength
Int.dia.
Capacity PK Cat. No.
cm mm gCHROMABONDfi Flash FM 15/2 SiOHCHROMABONDfi Flash FM 25/5 SiOHCHROMABONDfi Flash FM 25/10 SiOHCHROMABONDfi Flash FM 70/10 SiOHCHROMABONDfi Flash FM 70/20 SiOHCHROMABONDfi Flash FM 70/25 SiOHCHROMABONDfi Flash FM 150/25 SiOHCHROMABONDfi Flash FM 150/50 SiOHCHROMABONDfi Flash FM 150/70 SiOHCHROMABONDfi Flash FM 15/2 C18 ecCHROMABONDfi Flash FM 25/5 C18 ecCHROMABONDfi Flash FM 70/10 C18 ecCHROMABONDfi Flash FM 150/50 C18 ec
Silica adsorbents for low pressure column chromatographystandard silica 60, pore size ~ 60 Å; pore volume ~ 0.75 ml/g; spec. surface BET ~ 500 m2/ghighly porous, amorphous silicic acid in the form of hard, opalescent particles, prepared by precipitation of water glass withsulphuric acid.For higher demands on the performance of column packings we recommend our high-purity irregular Polygoprep silicas.silica FIA for the fluorescence indicator adsorption procedure for the determination of hydrocarbon groups in the testing of liquid fuels in accordance with DIN51791 and ASTM D 1319-58TThe FIA method determines saturated hydrocarbons, olefins and aromatic hydrocarbons of a sample chromatographically by adsorption and desorption in acolumn filled with FIA silica, in the presence of a fluorescent dye mixture
MACHEREY-NAGEL
Description particle size Weight PK Cat. No.mesh/mm kg
Aluminium oxide adsorbents for column chromatographyaluminium oxides produced by dehydration of different aluminium hydroxides, e. g. hydrargillite between 400 and 500°C,activity grade I, particle size 50 to 200µm, specific surface (BET) ~ 130m²/g MACHEREY-NAGEL
Safety CapsThe integral air filter blocks hazardous vapours and cleans the inflowing air from dustand dirt particles. With various connectors for capillaries and tubes. Safety Caps areavailable for most common glass bottles (e.g. thread size GL45). They can be customized for other container sizes byusing different adapters. Especially recommended for HPLC use: Solvents stay clean and components of solvent mix-tures can not evaporate. Optimum protection against health hazards caused by evaporation and insufficient sealing.Key advantages:- no evaporation of hazardous gases- no contamination of solvents- no crimping of connection tubing- easy container exchange- no air intake (HPLC)- cost efficiency (save expensive solvents by avoiding evaporation)
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1
Safety CapsSafe solvent supply.With integral air filter vent. For maximum efficiency, we recommend that the vent is changed every 6 months. The filtermembrane absorbs dust and dirt particles to protect your solvent reservoirs. The vent is universally suitable for all Safety Caps: it also works with your cur-rent Safety Caps. Simply replace the old vent with the new one.
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Description Connections PK Cat. No.
Safety Caps I, GL45Safety Caps II, GL45Safety Caps III, GL45Safety Caps IV, GL45Safety Caps VI, GL45Safety Caps I, with 1 stopcock, GL45Safety Caps II, with 2 stopcocks, GL45Safety Caps III, with 3 stopcocks, GL45Safety Caps IV, with 4 stopcocks, GL45Safety Caps VI, with 6 stopcocks, GL45Safety Caps II, combined, GL45 (1with stopcock / 1 without stopcock)Safety Caps III, combined, GL45 (2 with stopcocks / 1 without stopcock)Safety Caps I, for 3/16", GL45Safety Caps II for NS 29/32 bottles
Pressure relief valve for SafetyCapsPressure relief valve for SafetyCaps, with integral air filter.The valve opens when the HPLC pump is operated, and allows air to flow into the bottle- this prevents a vacuum buiding up in the bottle. As soon as the pump stops, the membrane immediately seals shut and no dangerous solvent vapours canescape.
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Description PK Cat. No.
Pressure relief valve for SafetyCapsPressure relief valve for SafetyCaps (refill pack) 110 9.139 8644.005 886
www.haeberle-lab.de
SafetyCaps Basic fire-resistantFor GL45 necked solvent reservoirs in HPLC devices- fire-resistant- orange- optionally available with 1 to 6 connectors
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1
Description Connections PK Cat. No.
SafetyCap I GL45SafetyCap II GL45SafetyCap III GL45SafetyCap IV GL45SafetyCap VI GL45
1 x connector 3.2mm o.d.2 x connector 3.2mm o.d.3 x connector 3.2mm o.d.4 x connector 3.2mm o.d.6 x connector 3.2mm o.d.
11111
4.005 7704.005 7804.005 7814.005 7764.005 779
SafetyCaps with shutoff fire-resistantFor GL45-necked, solvent reservoirs in HPLC devices- with shutoff- fire-resistant- orange- optionally available with 1 to 6 connectors
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Description Connections PK Cat. No.
SafetyCap I GL45SafetyCap II GL45SafetyCap II GL45SafetyCap IV GL45SafetyCap VI GL45
1 x connector 3.2mm o.d. with shutoff2 x connectors 3.2mm o.d. each with shutoff3 x connectors 3.2mm o.d. each with shutoff4 x connectors 3.2mm o.d. each with shutoff6 x connectors 3.2mm o.d. each with shutoff
11111
4.005 7724.005 7854.005 7864.005 7774.005 778
Fire-resistant pressure relief valve, orange, for SafetyCapsThe valve opens when the HPLC pump is operated, and allows air to flow into the bottle- this prevents vacuum building up within the bottle. As soon as the pump stops, themembrane immediately seals shut and no dangerous solvent vapours can escape.
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Description PK Cat. No.
pressure relief valve SafetyCaps, with integral air filter 1 4.005 769
SafetyWasteCaps fire-resistant, orangeFor GL45 necked, solvent reservoirs in HPLC systems. Stopper systems - made up of acap and safety funnel (on request) - offer great flexibility in the area of application anduse. Through the use of pure PTFE and HDPE, SafetyWasteCaps are resistant to aggressive media, e.g. organic solvents, acids and bases.
1 x safety funnel with mesh sieve,1 x connector 2.3/3.2mm o.d.1 x connection tubing 6.4mm i.d.2 x connectors 2.3/3.2mm o.d.1 x connector 6.4mm i.d.3 x connectors 2.3/3.2mm o.d.4 x connectors 2.3/3.2mm o.d.1 x connector 6.4mm i.d.
Safety Waste Caps S-thread with tube connection- different thread diameters- optional 1 to 4 connections- exhaust filter- safety funnel- level control
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Thread Connections PK Cat. No.
S51S55S60/61S60/61S70/71S90
2 x connectors 2.3/3.2 mm o.d., 1 x connector 6.4 mm i.d.2 x connectors 2.3/3.2 mm o.d., 1 x connector 6.4 mm i.d.3 x connectors 2.3/3.2 mm o.d.2 x connectors 2.3/3.2 mm o.d., 1 x connector 6.4 mm i.d.2 x connectors 2.3/3.2 mm o.d., 1 x connector 6.4 mm i.d.4 x connectors 2.3/3.2 mm o.d., 1 x connector 6.4 mm i.d.
Exhaust air filters, fire-resistant, for Safety Waste CapsFire-resistant exhaust air filter, orange. For flammable liquids.1
ScatDescription PK Cat. No.
Exhaust air filter Size S (24g), fire-resistantExhaust air filter Size M (48g), fire-resistant 11 4.005 7824.005 784
1
Safety Waste CapsFor safe disposal of liquid waste.Safety Waste Caps have a connection for exhaust vent filter.The exhaust vent filter absorbs 99% of all volatile substances that can evaporate from the containers during solventdisposal. For optimum protection of health and environment. Safety Waste Caps are manufactured of pure PTFE andHDPE, ensuring maximum chemical resistance against organic solvents and other aggressive chemicals.Please order exhaust vent filter separately.
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Thread Connections PK Cat. No.
GL45GL45GL803 connectors Ø 2.3/3.2 mm o.d.2 connectors Ø 2,3/3.2 mm o.d., 1 tubing Ø 6.4 mm i.d.4 connectors Ø 2.3/3.2 mm o.d., 1 tubing Ø 6.4 mm i.d.
1119.139 8659.139 8669.139 869
www.haeberle-lab.de
Safety Waste CapsB-thread with tube connection.1
Scat1
Thread Connections PK Cat. No.
53B83B
4 x connector 2.3/3.2mm o.d.1 x connector 6.4 mm i.d.4 x connector 2.3/3.2mm o.d.1 x connector 6.4mm i.d.
11
4.005 4414.005 438
Charcoal filters for Safety Waste CapsThere are three sizes of absorbent charcoal filters (with capacity for 3, 6 or 9 monthsrespectively) available:2
Exhaust air filter for Safety Waste Caps with splash guardThe filter cleans the exhaust air of solvent vapours. With a specific surface area of1200m²/g, our special, activated charcoal-based, granulate is the optimum filtermedium for almost all solvent vapours - 99% of all volatile substances are absorbed.
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Size Filterpacking
Description PK Cat. No.g
Exhaust air filter S - smallExhaust air filter M - mediumExhaust air filter L - large2448100
with splash guardwith splash guardwith splash guard111
4.005 6344.005 6314.005 635
Exhaust air filter supply pack for Safety Waste CapsExhaust air filter supply pack (blue)
4Scat
Description Filterpacking
PK Cat. No.g
Supply pack SSupply pack M 2448 43 4.005 8844.005 885
Safety Waste Caps with safety funnelPress the locking mechanism to open the funnel when disposing liquid waste. Whenreleasing, the funnel will close automatically and seal the container safely. With integral exhaust filter, for optimum protection against hazardous vapours and gases. Each cap has different connectors for multiple capillaries.
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Thread Connections PK Cat. No.
S 50S 55S 60 / 61S 65S 70 / 7183BS 90S 95
2 x connectors 2.3/3.2mm o.d.2 x connectors 2.3/3.2mm o.d.2 x connectors 2.3/3.2mm o.d.4 x connectors 2.3/3.2mm o.d.4 x connectors 2.3/3.2mm o.d.4 x connectors 2.3/3.2mm o.d.4 x connectors 2.3/3.2mm o.d.4 x connectors 2.3/3.2mm o.d.
Safety Waste Caps with mechanical level control- proven SCAT technology for SafetyWasteCaps- different thread diameters- choice of 1 to 4 connections- exhaust filter- safety funnel- mechanical level control- option: SafeLock- supplied as a set with connectors
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5
Thread Connections PK Cat. No.
S55S60/61S902 x connectors 2.3/3.2mm o.d., 1 x connector 6.4 mm i.d.2 x connectors 2.3/3.2mm o.d., 1 x connector 6.4 mm i.d.4 x connectors 2.3/3.2mm o.d., 1 x connector 6.4 mm i.d.
1114.005 6124.005 6134.005 616
Side collectors for Safety Waste CapsSide collectors for tube connectors.1
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Description PK Cat. No.
2 x connectors 3.2 mm o.d., 1 x tube 6.4 i.d.3 x tube 6.4 mm i.d.2 x tube 6.4 mm i.d.3 x connectors 3.2 mm o.d.
1111
4.005 8594.005 8644.005 8659.139 888
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Collectors for Safety Waste CapsWith fittings and ferrules 3.2 mm.Straight µanifold for tubing connector.2
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Description PK Cat. No.
3 connection collector for 3 capillaries with 3.2mm o.d.8 connection collector for 8 capillaries with 3.2mm o.d. 11 9.139 8889.139 889
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www.haeberle-lab.de
Level control, electronic, contactlessMonitor up to 15 containers simultaneouslyExternal devices, e.g. pumps or valves, can additionally be controlled via a contactswitch (floating output contact). All signal boxes are delivered with power supply (110/230V).Disk sensorLevel control without contacting the contents of the container. The sensitivity of the sensor can be adjusted to diffe-rent wall thicknesses. Suitable for all containers made in glass and non-conductive plastic. Not suitable for stainlesssteel containers and canisters made of electro-conductive plastic.Cable for transmission to signal box and for the connection of peripheral devices.Combine the extension cable to monitor containers at a distance of up to 200 metres. Control the connected devicevia a switch cable with a potential free contact.
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Description Description PK Cat. No.
Full level SafetySet
Low level SafetySet
Signal box withdisk fill level senor (1 channel),220V EU power supply, signal cable 3 meters,Fastening material for sensorSignal box withdisk low level senor (1 channel),220V EU power supply, signal cable 3 meters,Fastening material for sensor
Safety funnel, electroconductive HDPEHDPE safety funnel, black, electroconductive.- earthing cable included- for all types of chemicals- different thread sizes- with or without mesh sieve- for barrels- supplied as a set with connectors
Connection systems DG Safety CapsDG safety caps are flexible connection systems which have been designed specially forhandling aggressive, gas-forming liquids.Available with 1, 2 or 3 connection ports with optional stopcocks, DG safety caps are particularly suitable for pharma-ceutical and chemical applications.- No dangerous gas emissions- No slipped tubes- No substance loss due to evaporation- Clean working environmentThe safety caps come complete with v-ring seal (inside diameter 3.2 mm) and are ready for immediate use.V-ring seals are optionally available for 2 additional tube sizes (1.6 and 2.3 mm external diameter) to suit your appli-cation.DG safety waste caps are ideal for safely collecting and storing liquid laboratory waste. With a choice of different con-nections and activated carbon filters, they increase safety for laboratory technicians.- Drip-free collection of used or contaminated media.- Charcoal filters prevent harmful gas emissions- Additional connections available for charcoal filter housings and vent tubing.
1DURAN Group
Description PK Cat. No.
DG Safety Cap I (1 port)DG Safety Cap II (2 ports)DG Safety Cap III (3 ports)DG Safety Cap I (1 port), with stopcockDG Safety Cap II (2 ports), with stopcocksDG Safety Cap III (3 ports), with stopcocksDG Safety Cap for NS 29/32DG Safety Waste GL 45DG Safety Waste GL 45 for leak hose
HPLC bottles, Duranfi complete system 4-port screw capFor sterile transfer of media or for feeding solvent to HPLC instruments.The bottle is pressure/vacuum resistant from -1 to +1.5 bar (tested by TÜV inaccordance with EN 1596, GS marked). Complete with a 4-port screw cap (autoclavable, reusable). Suitable for tubediameters of 1.6 mm and 3.2 mm.Spare parts are available individually. Further connection system components for the GL45 thread are available uponrequest (Tubing not included). With Retrace Code (Batch Identification), with certificate available via the internet.Neutral /Type I glass acc. to USP/EP.
1DURAN Group
Capacity PK Cat. No.ml5001000 11 9.072 526
9.072 525
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Accessories for connecting system for Duranfi GL 45 bottles (HPLC)2 3DURAN Group
2
3
Description PK Cat. No.
Screw cap HPLC GL 45,4 Port completeSpare set for HPLC screw capPressure equilization, 0,2 µm for 4-Port-cap,incl. membrane filterSpare membrane filterfor pressure equilization, 0,2 µmBottle 1000 ml, GL 45,Duran pressure resistant up to +1,5 barBottle 500 ml, GL 45,Duran pressure resistant up to +1,5 bar
Flexible connecting system for Duranfi GL 45 flasks (HPLC)Materials used: PP and PTFE. Flexible modular system. Four different tube diameters(1.6 mm; 3.0 mm; 3.2 mm and 6.0 mm) can be connected. Sterile pressureequalisation is possible through use of a membrane filter. Unused ports can be provided with a blind cap.Typical applications: safe transfer of liquid media within a closed and sterile system (evaporation is reduced).Ordering exampleYou would like to fit two bottles with a 3-port connection system. You would like to work with two different tube dia-meters (1.6 mm and 3 mm) and pressure equalization. Consequently, you need the following individual components:Screw cap GL 45 3-port (Art. No. 7.623 018) 1 pack (2 per pack);Screw cap GL 14 (Art. No. 6.227 781) 2 packs (2 per pack);Insert for screw cap GL14 1.6 mm inner diam. (Art. No. 6.229 494) 2 packs (1 per pack);Insert for screw cap GL14 3.0 mm inner diam. (Art. No. 6.229 495) 2 packs (1 per pack);Pressure equalization set (complete) (Art. No. 6.228 023) 2 packs (1 per pack).
1DURAN Group
1
Description PK Cat. No.
Scew cap GL 45, 2 port x GL 14Screw cap GL 45, 3 port x GL 14Screw cap GL 14 for tubing connectorLiner for GL 14 screw cap, 1.6 mm i.d. holeLiner for GL 14 screw cap, 3 mm i.d. holeLiner for GL 14 screw cap, 3.2 mm i.d. holeLiner for GL 14 screw cap, 6 mm i.d. holeScrew cap, GL 14, redPressure equalising set, 0.2 µm filter, for 2- / 3-Port screw capReplacement 0.2 µm membrane filter for pressure equalising setBottle 1000 ml, GL 45, Duran pressure resistant up to +1,5 barBottle 500 ml, GL 45, Duran pressure resistant up to +1,5 bar
Scrubber Adapter for BottlesConsisting of PTFE body with connecting nut and two lateral GL18 threaded necks, a300mm long FEP inlet tube and a gas distributor with frit. Easy in- and outlet of gas bymeans of rigid-walled tubing (e.g. PTFE) which can be connected to the threaded necks using Bola Laboratory ScrewJoints. Elastic tubing can be connected by means of hose connectors. Inlet tube can be individually shortened. Specialfeature: the body of the adapter can be turned independently from the connecting nut. This means, that the completely assembled adaptor can be removed and fixed onto another bottle without the risk ofdisarranging the tubing. Suitable for bottles from Duran Group (formerly Schott, Mainz) with GL45 or GLS80 threadsand capacities between 100 and 5000ml.
2 3Bohlender
For thread type
Centre neck Lateral neck
PK Cat. No.
Thread GL45Thread GLS 80 11 9.110 3109.110 311
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Distributors for Reaction Vessels (R)Blue screw cap made of PP with GLS80 thread and PTFE body. Centre neck with GL25thread for connecting tubing up to 15 mm o.d., four lateral necks with GL18 thread.Exchangeable shaft guide (PTFE) with screw cap (PPS) for inserting and fixing a stirrer shaft in the centre neck included. Very good chemical resistance, for working temperatures up to max. +250°C (PP screw cap max. +140°C).
4Bohlender
For diam.stirrer shaft
PK Cat. No.
GL 25GL 25 4 x GL 184 x GL 18 8 mm10 mm 11 9.209 9209.209 921
Bottle distributorsPTFE, with GL-threaded necks and screw caps. Suitable for GL45 threaded bottles. Thedistributors are ideal for decanting liquids, whereby the necks are connected either to aliquid source or to an excess pressure or vacuum system (up to 6mm max. tubing dia.).For hassle-free connection, we recommend the Bola threaded connector, which has to be ordered separately, in accor-dance with the outer diameter of the tubing.
1Bohlender
Type Centralneck
PK Cat. No.
PFAPTFEPTFE2 x GL143 x GL143 x GL18
1119.209 8519.209 8529.209 844
1
Ground Joint GL AdaptersBlack screw cap made of PPS with GL45 thread or blue screw cap made of PP withGLS80 thread, movable insert with ground joint made of PTFE. Transition from a groundjoint to a glass thread. The body can be turned independently from the screw cap. The complete, assembled adaptercan be removed and fixed onto another bottle without the risk of disarranging the tubing. Very good chemical resistance, for working temperatures up to max. +250°C.
2Bohlender
Type Jointsize
PK Cat. No.NS
1 6.224 507
2
Flexible DistributorsPolypropylene. For connecting elastic tubing by simply slipping it onto hose connectors.Suitable for GL45 threaded bottles. With screw cap made of glass-fibre reinforced PP,ideal for transferring liquids or gases. The angled tubing connectors can be attached to a liquid source, or to a pressu-re or vacuum system and avoid crimping of the tubing. The distributor can be loosened by simply turning the screwcap. The complete distribution system can then be fixed onto another bottle.
3 4Bohlender
number of nozzles Fortubingbore
PK Cat. No.
mm232
6 - 9 mm6 - 9 mm7 - 11 mm111
7.634 8929.209 8589.209 859
3
4
GL fittingsPTFE couplings with GL connector screwthread. Connect PTFE, PFA, FEP, glass and metaltubing. Fittings are chemical and temperature resistant to approx. 250°C. Can be usedat working pressures of up to 10 bar when used in combination with Bola laboratory screwthread connections (at room temperature). A further advantage is that they can be used as expansion/reduction adapters to connecttubing of different diameters. For easy tubing connection we recommend accessory Bola laboratory screwthread con-nectors which can be ordered separately.
Optimafi 1high performance capillary columns100% dimethylpolysiloxanenonpolar phaseseparation of components according to boiling pointsthick film columns ≥ 3 µm film are expecially recommended for solvent analysissimilar phases: Permabondfi SE-30 , OV-1, DB-1, SE-30, HP-1, SPB-1, CP-Sil 5 CB, Rtx-1, 007-1, BP1, MDN-1, AT-1,ZB-1, OV-101for columns with 0.2 to 0.32mm ID and films <3µm the max. temperature for isothermal operation is 340°C, themax. temperature for short isotherms in a temperature programme is 360°Cfor 0.53mm ID columns with films < 3µm the max. temperatures are 320 and 340°C, resp.for thick film columns with films ≥ 3µm the max. temperatures are 300 and 320°C, resp.
1MACHEREY-NAGEL
1
Type Filmthickness
Length PK Cat. No.
µm m0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.) 0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)
In addition to this standard programme we will be happy to supply columns custom-made to your specifications.Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules.Column ends are melted or closed with septa, and thus protected from Additionally, we supply the corresponding test mixture with each column.
Optimafi 1 MS Accent ultra-low bleed capillary columns for GC
100 % Dimethylpolysiloxannonpolar phase with ultra-low bleeding, ideal for ion trap and quadrupol MS detectorsincreased sensitivity due to an unmatched low background level perfect inertness for basic compounds solvent rinsing for removal of impurities applicableapplication areas: all-round phase for environmental analyses, trace analyses, EPA methods, pesticides, PCB, food and drug analysessimilar phases: Ultra-1, DB-1 MS, HP-1 MS, Rtx-1 MS, Equity?1, AT-1 MS, VF-1 MS, CP-Sil 5 CB MSmax. temperature for isothermal operation 340°C, max. temperature for short isotherms in a temperature programme 360°C
1
MACHEREY-NAGEL
1
Type Filmthickness
Length PK Cat. No.
µm m0.25 mm i.d. (0.4 mm a.d.)0.25 mm i.d. (0.4 mm a.d.)0.25 mm i.d. (0.4 mm a.d.)0.25 mm i.d. (0.4 mm a.d.)0.32 mm i.d. (0.5 mm a.d.)0.32 mm i.d. (0.5 mm a.d.)0.32 mm i.d. (0.5 mm a.d.)0.32 mm i.d. (0.5 mm a.d.)
Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules. Column ends are melted or closed with septa, and thus protected from atmosphericoxygen.Additionally, we supply the corresponding test mixture with each column.
EPA 8140 / 8141 / 8141 A org. P pesticides
Column: OPTIMA®
mix A + 8141 OP pesticides calibration mix B; IS triphenyl phosphate and tributyl phosphate
Injection splitless (for 1 min); inj. temperature 250 °C Carrier gas He, 1 ml/min, constant pressureTemperature 100 °C 180 °C, 10 °C/min (2 min) 300 °C, 18 °C/min (3 min)
Optimafi 5 capillary columns for GC5 % phenyl - 95 % dimethylpolysiloxaneUSP G27, G36nonpolar standard phase with large range of applicationsimilar phases: Permabondfi
SE-52 , SE-54, SE-52, DB-5, HP-5, SPB-5, CP-Sil 8, Rtx-5, 007-5, BP5, MDN-5, AT-5, ZB-5for columns with 0.1 to 0.32mm ID and films <3µm the max. temperature for isothermal operation is 340°C, the max. temperature for short isotherms in a temperature programme is 360°Cfor 0.53mm ID columns with films < 3µm the max. temperatures are 320 and 340°C, resp.for thick film columns with films ≥ 3µm the max. temperatures are 300 and 320°C, resp.
1MACHEREY-NAGEL
1
Type Filmthickness
Length PK Cat. No.
µm m0.10 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)
In addition to this standard programme we will be happy to supply columns custom-made to your specifications.Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules. Column ends are melted or closed with septa, and thus protected from atmospheric oxygen.Additionally, we supply the corresponding test mixture with each column.
Optimafi 5 MS capillary columns for GC5 % diphenyl - 95 % dimethylpolysiloxaneUSP G36nonpolar phase with low bleedingideal for GC/MS and ECD applications and general analyses at trace levelperfect inertness for basic compoundssimilar phases: DB-5 MS, HP-5 MS, Ultra-2, Equity-5, CP-Sil 8 CB low bleed/MS, Rtx-5SIL-MS, Rtx-5 MS, 007-5 MS,BPX5, MDN-5S, AT-5 MS, VF-5 MSmax. temperature for isothermal operation: 340°C,max. temperature for short isotherms in a temperature programme: 360°C
1MACHEREY-NAGEL 1
Type Filmthickness
Length PK Cat. No.
µm m0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)
Optimafi 5 MS Accent capillary columns for GCsilarylene phasewith polarity similar to a 5 % diphenyl -95 % dimethylpolysiloxane phase.USP G27, G36lowest column bleed, nonpolar phase, ideal for ion trap and quadrupol MS detectors solvent rinsing for removal ofimpurities applicable application areas: all-round phase for environmental analyses, trace analyses, EPA methods,pesticides, PCB, food and drug analysessimilar phases: DB-5 MS, HP-5 MS, Ultra-2, Equity-5, CP-Sil 8 CB low bleed/MS, Rtx-5SIL-MS, Rtx-5 MS, 007-5 MS,BPX5, MDN-5S, AT-5 MS, VF-5 MSmax. temperature for isothermal operation: 340°C,max. temperature for short isotherms in a temperature programme: 360°C
1MACHEREY-NAGEL
1
Type Filmthickness
Length PK Cat. No.
µm m0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)
Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules. Column ends are melted or closed with septa, and thus protected from atmosphericoxygen.Additionally, we supply the corresponding test mixture with each column.
® 5-MS Accent
with a conventional 5-MS phase shows the outstanding
performance of the silarylene phase.
Background noise at 340 °C
®
5 MS Accent, which is approximately three times lower
increased sensitivity and allows the application in trace
Optimafi 17 capillary columns for GCphenylmethylpolysiloxane (50 % phenyl)USP G3medium polar phase suitable for higher temperatures preferred applications: steroids, pesticides, drug analyses similar phases: OV-17, DB-17, HP50+, HP17, SPB-50, SP-2250, Rtx-50, CP-Sil 24 CB, 007-17, ZB50max.temperature for isothermal operation: 320°C, max. temperature for short isotherms in a temperature programme: 340°C, for 0.53mm ID columns the max. temperatures are 300 and 320°C, resp.
1MACHEREY-NAGEL 1
Type Filmthickness
Length PK Cat. No.
µm m0.10 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)
Optimafi 1701 capillary columns for GC14 % Cyanopropyl-phenyl - 86 % DimethylpolysiloxaneUSP G46medium polar phasespecial selectivity due to high cyanopropyl contentreference column for structure identification, e.g. in combination with Optima 5film thickness ? 1 µm for solvent analysessimilar phases: OV-1701, DB-1701, CP?Sil 19 CB, HP-1701, Rtx-1701, SPB-1701, 007-1701, BP10, ZB-1701max. temperature for isothermal operation: 300°C, max. temperature for short isotherms in a temperature programme: 320°Cfor 0.53mm ID columns the max. temperatures are 280 and 300°C, resp.
1MACHEREY-NAGEL
1
Type Filmthickness
Length PK Cat. No.
µm m0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.32.mm i.d. (0.5 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)
Optimafi 624 capillary columns for GC6 % cyanopropyl-phenyl - 94 % dimethylpolysiloxaneUSP G43medium polar phaserecommended for environmental analysessimilar phases: HP-624, HP-VOC, DB-624, DB-VRX, SPB-624, CP-624, Rtx-624, Rtx-Volatiles, 007-624, BP624, VOCOLmax. temperature for isothermal operation: 280°C, max. temperature for short isotherms in a temperature programme: 300°C
1MACHEREY-NAGEL
1
Type Filmthickness
Length PK Cat. No.
µm m0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)
In addition to this standard programme we will be happy to supply columns custom-made to your specifications.
Optimafi 624 LB capillary columns for GC6 % cyanopropyl-phenyl - 94 % dimethylpolysiloxaneUSP G 43medium polar phaselow bleed columns for halogenated hydrocarbons, volatiles, aromatic compounds, solvents etc.similar phases: HP-624, HP-VOC, DB-624, DB-VRX, SPB-624, CP-624, Rtx-624, Rtx-Volatiles, 007-624, BP624, VOCOLmax. temperature for isothermal operation: 280°C, max. temperature for short isotherms in a temperature programme: 300°C
2MACHEREY-NAGEL
2
Type Filmthickness
Length PK Cat. No.
µm m0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)
1.801.80
3050
11
4.003 2144.003 215
In addition to this standard programme we will be happy to supply columns custom-made to your specifications.
OPTIMAfi 225 capillary columns for GC50 % cyanopropylmethyl - 50 % phenylmethylpolysiloxaneUSP G7/G19middle polar phaserecommended for fatty acid analysessimilar phases: DB-225, HP-225, OV-225, Rtx-225, CP-Sil 43, 007-225, BP225max. temperature for isothermal operation: 260°C, max. temperature for short isotherms in a temperature programme: 280°C
1MACHEREY-NAGEL
1
Type Filmthickness
Length PK Cat. No.
µm m0.10 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)
Optimafi WAX capillary columns for GCpolyethylene glycol 20000 daltonsUSP G16polar phaserecommended for solvent analysis and alcoholssuitable for aqueous solutionssimilar phases: Premabondfi CW 20 M , DB-Wax, Supelcowax, HP-Wax, HP-INNOWAX, Rtx-Wax, CP-Wax 52 CB,Stabilwax, 007-CW, BP20, AT-Wax, ZB-Waxmax. temperature for isothermal operation 250°C, max. temperature for short isotherms in a temperature programme: 260°C,for 0.53 mm ID columns the max. temperatures are 220 and 240°C, resp.
1MACHEREY-NAGEL
1
Type Filmthickness
Length PK Cat. No.
µm m0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)
Optimafi FFAP capillary columns for GCpolyethylene glycol 2-nitroterephthalateclose equivalent USP G25/G35polar phaserecommended for FAME, free carboxylic acidssimilar phases: Permabond FFAP , DB-FFAP, HP-FFAP, CP-Sil 58 CB, 007-FFAP, CP-FFAP CB, Nukolfor columns with 0.10 to 0.32mm ID the max. temperature for isothermal operation is 250°C, the max. temperaturefor short isotherms in a temperature programme is 260°Cfor 0.53mm ID columns the max. temperatures are 220 and 240°C, resp.
1MACHEREY-NAGEL
1
Type Filmthickness
Length PK Cat. No.
µm m0.10 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)0.53 mm i.d. (0.8 mm o.d.)
In addition to this standard programme we will be happy to supply columns custom-made to your specifications.Each column is individually tested and supplied with test certificate and test chromatogram, but without fittings or ferrules. Column ends are melted or closed with septa, and thus protected from atmosphericoxygen.Additionally, we supply the corresponding test mixture with each column.
FAME test
Column: OPTIMA®
Carrier gas: 1.2 bar He, splitTemperature: 55 °C 250 °C,
Optimafi 5 Amine GC capillary columns for amine separationespecially deactivated for the analysis of polyfunctional amines such as ethanolamines, amino-functionalised diols and similarcompounds, which are important base materials in industrial chemistry, and shows strong tailing on standard-deactivatedcolumns similar phases: Rtx-5 Amine, PTA-5USP G27/G36improved linearity for analyses of active components at trace levels: no amine absorptions even for aliphatic and aromatic amines at concentrations of 100pg/peak tested with the Optimafi Amine test mixture, which among others also contains diethanolamine and propanol-pyridine (this test mixture is suppliedwith each column)max. temperature for isothermal operation: 300°C, max. temperature for short isotherms in a temperature programme: 320°C
MACHEREY-NAGEL
Type Filmthickness
Length PK Cat. No.
µm m0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)0.32 mm i.d. (0.5 mm o.d.)
Injection volume: 1 µlCarrier gas: 0,6 bar H2, Split 1:100Temperature: 100 °C (3 min) 280 °C, 10 °C/min
1
2
3
4
567
89
10
11
12
0 10 20 min
Peaks:
3. Triethylaminen-propylaminen-butylamine
6. Tri-n-propylamine
8. Tri-n-butylamine
12. Tri-n-hexylamine
MN Appl. No. 210280
Capillary columns for special separationsLIPODEX® cyclodextrin phase for enantiomer separationbase material: cyclic oligosaccharide consisting of eight glucose units bonded through α-1.4-linkages (y-cylodextrin)regioselective alkylation and acylation of the hydroxyl groups leads to a lipophilic phase, which is well suited for GC enantiomer analysesimportant advantage: many compounds can be analysed without derivatisation (however, for certain substances enantioselectivity can be favourably influenced by formation of derivatives)A large number of separations have been achieved, however, it is not possible to make gerneral predictions. Even for compounds with small structural diffe-rences or within a homologous series the enantiodifferentia-tion can be quite different.LIPODEX fi is patented under EP 0407 412 and US Re. 36.092
MACHEREY-NAGEL
Lipodexfi E � octakis-(2.6-di-O-pentyl-3-O-butyryl)-γ-cyclodextrinrecommended for α-amino acids, α- and β-hydroxycarboxylic acid esters, alcohols (TFA), diols (TFA), ketones, pheromones(cyclic acetals), amines, alkyl halides, lactonesmax. temperature for isothermal operation: 200°C, max. temperature for short isotherms in a temperature programme: 220°C
MACHEREY-NAGEL
Type Diam. Length PK Cat. No.m
FS-LIPODEXfi EFS-LIPODEXfi E
0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)
2550
11
4.002 9254.002 926
Enantiomer separation of amino acid methyl esters (TFA)
heptakis-(2.3-di-O-methyl-6-O-t-butyldimethyl-silyl)-β-cyclodextrinphase diluted with optimised polysiloxanerecommended for γ-lactones, cyclopentanones, terpenes, esters, tartratesmax. temperature for isothermal operation: 230°C, max. temperature for short isotherms in a temperature programme: 250°C
MACHEREY-NAGEL
Type Diam. Length PK Cat. No.m
FS- HYDRODEX β− 6 TBDMFS- HYDRODEX β− 6 TBDM
0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)
2550
11
4.002 9314.002 932
heptakis-(2.3-di-O-acetyl-6-O-t-butyldimethyl-silyl)-β-cyclodextrinall columns 0.25 mm ID, 0.4 mm ODphase diluted with optimised polysiloxanerecommended for alcohols, esters, ketones, aldehydes, δ-lactonesmax. temperature for isothermal operation: 220°C, max. temperature for short isotherms in a temperature programme: 240°C
MACHEREY-NAGEL
Type Diam. Length PK Cat. No.m
FS-HYDRODEX β-TBDAC
FS-HYDRODEX β-TBDAC
0.25 mm i.d. (0.4 mm o.d.)0.25 mm i.d. (0.4 mm o.d.)
2550
11
4.002 9354.002 936
max. temperature 250 °C
Peaks:
4. Naproxen
5. Ketoprofen
1
SR
2
3
4
5
10 15 20 30 40 50 min
2
2Cl2
Peaks:
1
2
3
4
0 10 20 30 min
O
OH
Reagents and methods for derivatisationDerivatisation reagentsfor improved volatility, better thermal stability or low limit of detection in gas chromatographyprerequisite: quantitative, rapid and reproducible formation of only on derivativehalogen atoms introduced by derivatisation (e.g. trifluoroacetates) allow specific detection (ECD) with the advantage of high sensitivity elution order and frag-mentation patterns in MS can be influenced by a specific derivatisationreagents for silylation - alkylation (methylation) - acylationare available
MACHEREY-NAGEL
HYDRODEX cyclodextrin phases for enantiomer separationcyclodextrin derivatives with high melting point:for GC-enantiomer separation diluted with polysiloxanes MACHEREY-NAGEL
Amino acids silylation R’ – CH(NH – TMS) – CO – O – TMS BSTFA, MSTFA
alkylation (a)
+ acylation (b)
R’ – CH(NH – CO – R) – CO – O – R a) TMSH
b) HFBA, MBTFA
Carboxylic acids
(fatty acids)
silylation R’ – CO – O – TMS
susceptible to hydrolysis
MSTFA
alkylation R’ – CO – O – R TMSH
Carbohydrates silylation MSTFA
acylation MBTFA
Steroids acylation MBTFA, HFBA
Derivatisation method development kitWhich type of derivatisation is best suited for your sample (alkylation, acylation or silylation)? MACHEREY-NAGEL
Contents of the kit PK Cat. No.
2 x 1 ml each of TMSH, MSTFA, MBTFA 1 4.001 514
Acylation kitWhich is the proper reagent for acylation? MACHEREY-NAGELContents of the kit PK Cat. No.
2 x 1 ml each for MBTFA , TFAA, MBHFBA 1 6.207 118
Alkylation kitWhich is the proper reagent for methylation? MACHEREY-NAGELContents of the kit PK Cat. No.
3 x 1 ml each for TMSH, DMF-DMA 1 4.001 513
Silylation kitWhich is the proper reagent for silylation? MACHEREY-NAGELContents of the kit PK Cat. No.
2 x 1 ml each for MSTFA, BSTFA, TSIM, MSHFBA 1 6.704 458
Acylation reagents for GC - Anhydridesby-products of acylation with anhydrides: corresponding acidsexcess reagent and the acid formed have to be removedAcylation with fluorinated acid anhydrides can be used for alcohols, phenols, carboxylic acids, amines, amino acidsand steroids forming volatile, stable derivatives suited for FID as well as for ECD detection.Heptafluorobutyric acid anhydride (HFBA)m.w. 410.06, Bp 106 - 107°C (760 mm Hg),C3F7 - CO - O - CO - C3F7density d20°/4° = 1.665
Acylation with HFBA can be used for alcohols, phenols,
carboxylic acids, amines, amino acids and steroids form-
ing volatile, stable derivatives suited for FID as well as for
ECD detection.
Procedure:
Dissolve 0.1 to 1 mg of the sample in 0.1 ml solvent,
add 0.1 ml HFBA and heat to 60 – 70 °C for 1 – 2
hours. If the sample need not be concentrated prior
to the analysis and if there is no danger of catalytical-
ly induced side reactions, pyridine is used as solvent.
The reaction solution can be injected directly into the
gas chromatograph. Otherwise use a volatile solvent
and evaporate solvent, excess derivatisation reagent
and free acid in a stream of nitrogen. Dissolve the
residue in 50 µl hexane, chloroform etc. and inject
aliquot portions.MN Appl. No. 213042
This method is recommended for alcohols, primary and
secondary amines as well as thiols under mild, neutral
conditions. MBTFA also forms very volatile derivatives with
carbohydrates [J. Sullivan and L. Schewe, J. Chromatogr. Sci.
15 (1977) 196 – 197].
Procedure:
Add 0.5 ml MBTFA to about 2 mg sample. If there is
no reaction at ambient temperature, heat the reaction
mixtures. It is recommended to use a ratio of solvent
to MBTFA of 4 : 1. The reaction mixture can be chro-
matographed directly.MN Appl. No. 213051
Acylation reagents for GC - Bisacylamidesby-products: corresponding neutral acylamides, which can be easily removed due totheir high volatility; because of neutral conditions and favourable chromatographicproperties often removal of the bisacylamide is not necessary. Thus sample preparation is much more convenient.Acylation with fluorinated acid amides is recommended for alcohols, primary and secondary amines as well as forthiols under mild, neutral conditions. MBTFA also forms very volatile derivatives with carbohydrates.MBTFA /MBHFBAN-methyl-bis(trifluoroacetamide) MBTFAm.w. 223.08, Bp 123 - 124°C (760 mm Hg), density d20°/4° = 1.55CF3 - CO - N(CH3) - CO - CF3
MACHEREY-NAGEL
Code Capacity PK Cat. No.
MBTFAMBTFAMBTFA20 x 1 ml1 x 10 ml5 x 10 ml
20157.401 1437.510 7966.228 605
Due to their purpose, derivatisation reagents are very reactive substances. For this reason they should be stored cool and protected from moisture.Our derivatisation reagents are supplied in vials with crimp caps for easy access with a syringe. Vials with pierced sealing disks have limited stability andshould be used soon.
Alkylation reagents for GC - Trimethylsulphonium hydroxideTMSH (0.2 M in methanol) M.G. 94.06 MACHEREY-NAGEL
Code Capacity PK Cat. No.
TMSHTMSHTMSH10 x 1 ml20 x 1 ml5 x 10 ml
102057.086 1477.083 3084.001 512
Silylation reagentsUsually the term silylation in GC stands for replacement of active hydrogen atoms by atrimethylsilyl group (TMS derivative). Sometimes, however, trialkylsilyl groups ordimethylalkylsilyl groups with longer alkyl chains are used for derivatisation. The trialkylsilyl group increases volatilityand enhances thermal stability of the sample. Silylation can be catalysed either acidic by addition of TMCS or basic byaddition of pyridine (e. g. for sterically hindered functionalities like tert. alcohols)
MACHEREY-NAGEL
www.haeberle-lab.de
Procedure:
Add 0.5 ml silylation reagent to 1 – 10 mg sample; if necessary, add some solvent (normally pyridine or DMF
[dimethylformamide] are used). Heat to 60 – 80 °C for 20 min to increase the reaction rate.
Silylation reagents - BSTFA, SILYL-991N,O-bis-trimethylsilyl-trifluoroacetamidem.w. 257.4, Bp 40°C (12 mm Hg), density d20°/4° = 0.961BSTFA:R = CF3powerful trimethylsilyl donor with approximately the same donor strength as the non-fluorinated analogue BSAadvantage of BSTFA over BSA: greater volatility of its reaction products (particularly useful for GC of some lower boi-ling TMS amino acids.BSTFA is nonpolar (less polar than MSTFA), and can be mixed with acetonitrile for improved solubility. For silylatingfatty acid amides, hindered hydroxyls and other compounds, which are difficult to silylate (like secondary alcohols andamines), we recommend BSTFA + 1 % trimethylchlorosilane (TMCS), available under the designation SILYL-991.
Due to their purpose, derivatisation reagents are very reactive substances. For this reason they should be stored cool and protected from moisture.Our derivatisation reagents are supplied in vials with crimp caps for easy access with a syringe. Vials with pierced sealing disks have limited stability andshould be used soon.
Silylation reagents - MSTFAN-methyl-N-trimethylsilyl-trifluoroacetamidem.w. 199.1, Bp 70°C (75 mm Hg), density d20°/4° = 1.11MSTFA:R' = CF3, R" = CH3the most volatile trimethylsilyl amide availablevery strong TMS donor which does not cause any noticeable FID fouling even after long-time measuring seriesThe already good solution characteristics can be improved by addition of submolar quantities of protic solvents (e.g.TFA for extremely polar compounds such as hydrochlorides) or pyridine (e.g. for carbohydrates).recommended application: carboxylic acids, hydroxy and ketocarboxylic acids, amino acids, amines, alcohols, poly-alcohols, sugars, mercaptans and similar compounds with active hydrogen atoms. Even amine hydrochlorides can besilylated directly.advantages: complete reaction with high reaction rates, even without a catalyst (1 to 2% TMCS or TSIM)the by-product of the reaction (N-methyltrifluoroacetamide) features high volatility and short retention time.
Due to their purpose, derivatisation reagents are very reactive substances. For this reason they should be stored cool and protected from moisture.Our derivatisation reagents are supplied in vials with crimp caps for easy access with a syringe. Vials with pierced sealing disks have limited stability andshould be used soon.
1
Silylation with MSTFA
Procedure:
Dissolve 10 – 15 mg sample in 0.8 ml solvent, then add 0.2 ml MSTFA. The reaction mixture can be heated to
60 – 70 °C for up to 1 h and can be analysed directly. If TFA is used as a solvent, proceed as follows [M. Donike,
J. Chromatogr. 85 (1973) 1 – 7]: Dissolve 1 – 2 mg sample in 100 µl TFA. Dropwise add 0.9 ml MSTFA.
After cooling the sample can be chromatographed directly.MN Appl. No. 213111
Chromatography columns, ground glass jointWith PTFE stopcock.With NS ground socket neck as indicatedIndents are moulded in above the stopcock for holding cotton wool plug supports.
Ion exchange papersA thin (0.20 mm) DEAE cellulose paper-a weakly basic anion exchanger withdiethylaminoethyl functional groups. The ion exchange capacity is 1.7 µeq/cm2 and flowrate is 95 mm/30 min. For use with reverse transcriptase assays and DNA polymerase.
2Whatman
Grade Dia. PK Cat. No.mm
DE81 23 400 9.950 335
2
www.haeberle-lab.de
MACHEREY-NAGEL
MACHEREY-NAGEL
Professional Solutions for HPLC
➢ C18 and C8 Gravity unique base-deactivation for pH extremes
➢ C18 Pyramid polar endcapped phase
➢ Sphinx RP distinct selectivity for aromatic compounds
➢ C18 Isis exceptional steric selectivity
www.mn-net.com
Products for Solid Phase Extraction
➢ modern polymer resins – HR-X, EASY, PS/DVB copolymers
➢ standard phases – C18ec, SiOH, NH2, . . .
➢ cation / anion exchangers – HR-XC, HR-XA, SA, SB
➢ polar phases – Alox, Diol, Florisil®, . . .
➢ phases for special applications – QuEChERS, PCB, PAH, THC, . . .
guaranteed by stringent production control including
tion
coatings or impregnations are not necessary
Florisilfi adsorbent for column chromatographyhard granular magnesia silica gel: MgO 15.5±0.5% - SiO2 84.0±0.5% - Na2SO4 <=1.0%; 60/100 mesh typical applications: sample preparation (SPE); clean-up of pesticide residues, separation of chlorinated pestici-des, extraction of steroids, sex hormones, antibiotics, lipids etc.
MACHEREY-NAGEL
Description particle size Weight PK Cat. No.mesh/mm kg
Florisil standard 60 / 100 meshFlorisil standard 60 / 100 mesh 0.15 / 0.25 mm0.15 / 0.25 mm 15 11 6.232 8734.005 002
www.haeberle-lab.de
Standard silica layers for TLCSupport materials for TLC ready-to-use layersGlass plates: glass, ~ 1.3 mm thick, high requirments for weight, packaging and storage, ideal torsional strength,high temerature stability, susceptible to breakage, can not be cut with scissors, high resistance against solvents,mineral acids and conc. ammonia, suitability for aqueous detection reagents depends on the phasePOLYGRAM®: polyester, ~ 0.2 mm thick, low requirments for weight, packaging and storage, low torsional strength,max. 185°C temerature stability, not susceptible to breakage, can be cut with scissors, high resistance against solvents, mineral acids and conc. ammonia, very suitable for aqueous detection reagentsALUGRAM®: aLuminium, ~ 0.15 mm thick, low requirments for weight, packaging and storage, relatively high torsional strength, high temerature stability, not susceptible to breakage, can be cut with scissors, high resistanceagainst solvents, low resistance against mineral acids and conc. ammonia, limited suitable for aqueous detectionreagents
Adamant unmodified standard silica layers for TLCsilica 60, specific surface (BET) ~ 500m²/g, mean pore size 60Å, specific pore volume 0.75ml/g,particle size 5 to 17µmoutstanding hardness and abrasion resistance due to an optimized binder systemincreased separation efficiency due to an optimized particle size distirbutionhigh suitability for trace analyses resulting from a UV indicator with brilliance and a low-noise background of the layeravailable as glass plates with or without fluorescent indicator (UV254)
MACHEREY-NAGEL
Type Plate size Thickness of layer PK Cat. No.cm mm
SIL G unmodified standard silica layers for TLCglass plates, Polygramfi, Alugramfi
silica 60, specific surface (BET) ~ 500m²/g, mean pore size 60 Å, specific pore volume 0.75 ml/g,particle size 5 to 17µm; standard grade thickness of layer for analytical plates 0.25mm, for preparative plates 0.5 and 1mm; for 2mm preparative layers aslightly coarser material is used indicators: manganese activated zinc silicate with green fluorescence for short-wave UV (254nm); special inorganic fluorescent pigment with blue fluorescence for long-wave UV (366nm)binders: highly polymeric products, which are stable in almost all organic solvents and resistant towards aggressive visualisation reagents; binder system forPolygramfi sheets is also completely stable in purely aqueous eluents available as glass plates, Polygram polyester sheets and Alugram aluminium sheetsavailable as glass plates with or without fluorescent indicator (UV254)
1 2 3MACHEREY-NAGEL
1 2 3
Type Plate size Thickness of layer PK Cat. No.cm mm
Glass plates SIL G-25 UV254Glass plates SIL G-25Glass plates SIL G-25Glass plates SIL G-25Glass plates SIL G-25Glass plates SIL G-25Glass plates SIL G-25 UV254Glass plates SIL G-25 UV254Glass plates SIL G-25Glass plates SIL G-25 UV254Glass plates SIL G-25Glass plates SIL G-25 UV254Glass plates SIL G-100Glass plates SIL G-100 UV254Glass plates SIL G-200Glass plates SIL G-200 UV254POLYGRAMfi polyester sheets SIL GPOLYGRAMfi polyester sheets SIL G UV254POLYGRAMfi polyester sheets SIL GPOLYGRAMfi polyester sheets SIL G UV254POLYGRAMfi polyester sheets SIL GPOLYGRAMfi polyester sheets SIL G UV254POLYGRAMfi polyester sheets SIL GPOLYGRAMfi polyester sheets SIL G UV254POLYGRAMfi polyester sheets SIL GPOLYGRAMfi polyester sheets SIL G UV254ALUGRAMfi aluminium sheets SIL G UV254ALUGRAMfi aluminium sheets SIL G UV254ALUGRAMfi aluminium sheets SIL GALUGRAMfi aluminium sheets SIL G UV254ALUGRAMfi aluminium sheets SIL GALUGRAMfi aluminium sheets SIL G UV254ALUGRAMfi aluminium sheets SIL GALUGRAMfi aluminium sheets SIL G UV254ALUGRAMfi aluminium sheets SIL GALUGRAMfi aluminium sheets SIL G UV254ALUGRAMfi aluminium sheets SIL GALUGRAMfi aluminium sheets SIL G UV254
2.5 x 7.55 x 105 x 105 x 105 x 105 x 205 x 2010 x 1010 x 2010 x 2020 x 2020 x 2020 x 2020 x 2020 x 2020 x 202.5 x 7.52.5 x 7.54 x 84 x 85 x 205 x 2020 x 2020 x 2040 x 2040 x 202.5 x 7.54 x 85 x 7.55 x 7.55 x 105 x 105 x 205 x 2010 x 2010 x 2020 x 2020 x 20
Silgur unmodified standard silica layers with concentrating zoneglass plates silica 60, specific surface (BET) ~ 500m²/g, mean pore size 60Ä, specific pore volume 0.75ml/g, particle size 5.17µmkieselguhr zone for rapid sample application: because kieselguhr is completely inert towards a large number of compounds, the samples always from anarrow band at the interface of the two adsorbents, irrespective of shape, size or position of the spots in the concentrating zone. Separation then takes placein the silica layer.available as glass plates with or without fluorescent indicator (UV254)
MACHEREY-NAGEL
Type Plate size Thickness of layer PK Cat. No.cm mm
Nano-Adamant unmodified nano silica layers for HPTLCglass platesnano silica 60, specific surface (BET) ~ 500m²/g, mean pore size 60Å, specific pore volume 0.75ml/g,particle size 2 to 10 µmoutstanding hardness and abrasion resistance due to an optimized binder system increased separation efficiency due to an optimized particle size distributionhigh suitability for trace analyses resulting from a UV indicator with increased brilliance and a low-noise background of the layer narrow fractionation of thesilica particles allows theoretical plate heights, which are one order of magnitude smaller than on standard silica layers with the advantage of sharper separations, shorter developing times, shorter migration distances, lower amount of samples, and increased detection sensitivity with equal selectivityavailable as glass plates with or without fluorescent indicator (Υς 254)
MACHEREY-NAGEL
Type Plate size Thickness of layer PK Cat. No.cm mm
Nano-SIL unmodified Nano-silica layers for HPTLCAlugramfi
nano silica 60, specific surface (BET) ~ 500m²/g, mean pore size 60Å, specific pore volume 0.75ml/g,particle size 2 to 10µmindicator: manganese activated zinc silicate with green fluorescence for short-wave UV (254nm)binder: highly polymeric product, which is stable in almost all organic solvents and resistant towards aggressivevisualisation reagents narrow fractionation of the silica particles allows sharper separations, shorter developing times,shorter migration distances, smaller samples and an increased detection sensitivity compared to SIL G platesavailable as glass plates with or without fluorescent indicator (Υς254)
1MACHEREY-NAGEL
1
Type Plate size Thickness of layer PK Cat. No.cm mm
ALUGRAM NANO-SIL GALUGRAM NANO-SIL G UV254ALUGRAM NANO-SIL GALUGRAM NANO-SIL G UV254
5 x 205 x 2020 x 2020 x 20
0.200.200.200.20
50502525
4.005 0446.227 9006.227 8994.005 045
RP-18 W/UV254 octadecyl-modified nano silica layers for HPTLCglass plates, ALUGRAMfi
base material:silica 60, specific surface (BET) ~ 500m²/g, mean pore size 60Å, specific pore volume 0.75ml/g, mean particle size 9µm, pH stability 2 to 10indicator:acid-resistant product with a pale blue fluorescence for short-wave UV (254nm); UV-absorbing substances apppear asdark-blue to black spots on a light-blue backgroundpartial octadecyl modification, wettable with water, carbon content 14%order of polarity:silica > DIOL > NH2 > CN > RP-2 > C 18-50 > RP-18 W > C 18-100normal phase or reversed phase separation modes with eluents from anhydrous solvents to mixtures with high con-centrations of water (see figure); the relative polarity of the eluent determines the polarity of the layerrecommended application: aminophenols, barbiturates, preservatives, nucleobases, polycyclic aromatic hydrocar-bons, steroids, tetracyclines, plasticizers (phthalates)available as glass plates with or without fluorescent indicator (UV254)
2 3MACHEREY-NAGEL
2
3
Type Plate size Thickness of layer PK Cat. No.cm mm
Glass plates RP-18 W UV254Glass plates RP-18 W UV254Glass plates RP-18 W UV254Glass plates RP-18 W UV254Glass plates RP-18 W UV254ALUGRAMfi aluminium sheets RP-18 W UV254ALUGRAMfi aluminium sheets RP-18 W UV254ALUGRAMfi aluminium sheets RP-18 W UV254ALUGRAMfi aluminium sheets RP-18 W UV254ALUGRAMfi aluminium sheets RP-18 W UV254
5 x 2010 x 1010 x 2020 x 2020 x 204 x 85 x 105 x 2010 x 1020 x 20
TLC plates, aluminium coatedStandard, rigid TLC plates in a choice of media, backing materials, and with dimensionsas indicated.1
MACHEREY-NAGEL1
Material Gelthickness
Dimensions PK Cat. No.
mm mmpolyester with aluminium oxide*aluminium with aluminium oxide* 0.20.2 200 x 200200 x 200 2525 9.003 495
9.003 498*with 254 nm UV indicator
TLC micro-set A beginner's set for science educationFeatures separations with simple developing sovents; samples are coloured thuseliminating the need for visualisation. All equipment needed is contained in the kit. MACHEREY-NAGEL
Description PK Cat. No.
TLC micro-set A for beginners 1 6.057 472
TLC micro-set A for beginners
This kit contains all chemicals and accessories for the fol-
lowing separations:
separation of the fat-soluble (lipophilic) test mixture
1: butter yellow, indophenol, sudan blue II, sudan
red G
separation of a mixture of anthraquinone dyes (test
dye mixture 2): blue 1, blue 3, green, greenish blue,
red, violet 1, violet 3
separation of a mixture of food dyes (test dye
mixture 3): brilliant black BN (E151), fast red E,
erythrosine (E127), yellow orange S (sunset yellow
CFC, E110), naphthol red S, ponceau 4 R (E124),
tartrazine (E102)
separation of dyes from felt tip pens
Contents of TLC micro-set A for beginners
1 manual
3 developing chambers
50 glass capillaries 1 µl
1 spotting guide
1 measuring cylinder10 ml
50 polyester sheets each 4 x 8 cm POLYGRAM® SIL G/UV254, ALOX N/UV254 and CEL 300
8 ml each of test dye mixture 1 (4 lipophilic dyes), test dyes sudan red G and sudan blue II
8 ml each of test dye mixture 2 (7 anthraquinone dyes), test dyes blue 1 and violet 2
8 ml each of test dye mixture 3 (7 food dyes), test dyes yellow orange S and brilliant black BN
100 ml each of toluene, toluene/cyclohexane (2:1, v/v)chloroform/acetone (1:1, v/v)2.5 % sodium citrate solution25 % ammonia/2-propanol (5:3, v/v)
TLC PlatesSilica gel plates. 250 µm layer. The plates are 20 x 20cm and can be cut with scissors.The flexible plates Silica-Gel 60 A, available on aluminium or polyester support material,have a similar separation performance to K6 glass plate and are often used for moderate to strong polar analytes.Ion exchanger plates (DEAE-diethylaminoethyl tertiary amine) are used for anionic species and are available on apolyester support material.
TLC platesLinear- K: give rapid, precise recognition. The pre-adsorbent layer is thicker than thesilica layer and functions like a sponge that pre-concentrates the sample before it reactswith the silica layer. This allows the user to apply sample volumes that would not be possible with standard TLC-plates.The symbol for silica gel is K (from the German Kieselgel). followed by a classification designation:- K5 10-12 µm silica, with pore size 150 Å- K6 10-12 µm silica, with pore size 60 Å- High-performance silica is prefixed with the letters HP: HP-K 4.5 µm silica, pore size 60 Å- Reversed-phase plates with a bonded alkyl group are represented by a K, followed by the length of the alkyl chainKC-18 10-12 µm Silica 60 Å, octadecyl bonded phaseFurther identification information for a particular plate can be found from the following letter codes:- L pre-adsorbent layer for the easier application of larger volumes. Compresses each sample into a narrow horizontalband, so also known as Linear-K, prefix L.- D channelled plates with separate lanes. 2 mm clear glass channels separate the individual sample lanes to preventthe samples becoming mixed, so D for Division.- F fluorescent indicator: fluorescent plates glow bright green under UV light. Samples that absorb UV at 254 nm arerecognized by fluorescence quenching.- M microscope slide, plate size 25/75 mm.- P preparative layer. Has a thickness of 500 or 1000 µm for large samples.These letter codes enable easy, rapid identifcation of all TLC plates, for example PLK6DF = preparative K6 silica 60 Åpore diameter, separate lanes, fluorescent indicator and preadsorbent layer.
Chromatography paper/Ion exchange papersWhatman chromatography papers are the most widely used papers for chromatographyworldwide. This acceptance and usage reflects the purity, high quality and consistency ofWhatman papers. These qualities are relied upon by chromatographers and essential to successful reproducible chro-matography. Whatman chromatography paper media are made from specially selected cotton cellulose. They are rigo-rously quality controlled for characteristics important to the chromatographer and to ensure uniformity within thegrade.1 Chr world standard chromatography paper. A smooth surface, 0.18 mm thick with a linear flow rate (water) of 130mm/30 min. Good resolution for general analytical separations.3MM Chr widely used as a blotting paper, 3MM Chr is used in both electrophoresis and for general chemistry. A medi-um thickness paper (0.34 mm) used extensively for general chromatography and electrophoresis. Flow rate is 130mm/30 min.3 Chr medium thickness paper (0.36 mm) with a flow rate of 130 mm/30 min. For general applications with medi-um/heavy solute loadings. Frequently used for separation of inorganic compounds and for electrophoresis.17 Chr thick (0.92 mm) and highly absorbent paper with a very high flow rate of 190 mm/30 min. Suitable for theheaviest loadings and ideal for preparative paper chromatography and electrophoresis.Ion exchange papersDE81A thin (0.20 mm) DEAE cellulose paper-a weakly basic anion exchanger with diethylaminoethyl functional groups. Theion exchange capacity is 1.7 µeq/cm² and flow rate is 95 mm/30 min. For use with reverse transcriptase assays andDNA polymerase.SG81A unique paper (0.27 mm thick) combining cellulose and large pore silica gel. Suitable for separations in which bothpartition and adsorption are important, including the separation of phospholipids, steroids, phenols and dyes. Flowrate is 110 mm/30 min.P81A thin (0.23 mm) cellulose phosphate paper. Strong cation exchanger of high capacity. Ion exchange capacity is 18.0µeq/cm² and the flow rate is 125 mm/30 min. For use with protein kinase assay with peptide substrates.
100 x 300 mm200 x 200 mm250 x 250 mm460 x 570 mm200 x 200 mm315 x 355 mm460 x 570 mm460 x 570 mm580 x 680 mm460 x 570 mm460 x 570 mm460 x 570 mm460 x 570 mm460 x 570 mm
Standard separating chamberWith absolutely flat, chamber floor, ground flange rim and lid for all TLC plates up to 200 x 200 mm.Other separating chambers available on request.
2
Type PK Cat. No.
Separating chamber with knob lidSeparating chamber with ground cover plateKnob lid for 9.020 160Glass cover disc for 9.020 173
1111
9.020 1609.020 1739.020 1639.020 177
2
Simultaneous developing chamberFor TLC plates up to 20 x 20 cm. Choice of plates as indicated.3
MACHEREY-NAGEL3
Chromatography stripsDivided into 12 bands, each of 15 mm wide, for parallel separation of 12 samples. Whatman
Grade Width Length PK Cat. No.mm mm
1Chr CRL 110 213 100 9.950 321
Chromatography paper, reelsChromatography Paper 1 CHRThe standard chromatography paper. Good resolution for general analytical separations.Pure cellulose. Thickness 0.18 mm. Capillary rise (water) 130 mm/30 min.Chromatography Paper 3MM CHRUsed in electophoresis, in general chemistry and as blotting paper. Pure cellulose. Thickness 0.34 mm.Capillary rise (water) 130 mm/30 min.
Simultaneous batch separating chamberWith absolutely flat, chamber floor, ground flange rim and lid and storage trough for five 200 x 200 mm TLC plates.1 1
Type PK Cat. No.
Simultaneous batch separatingchamber with knob lidSimultaneous batch separatingchamber with ground cover plate
11
9.020 1679.020 174
Nano separating chambersThe use of quantitative TLC on nano or HPTLC gel layer platesis increasing. Nano separating chambers have been developed for the more popular 100 x 100 mm and 200 x 100mm plate formats and have all the advantages of standard separating chambers.
2 2
Type PK Cat. No.
Nano separating chamber 200 x 100 mm,with stainless steel lidNano separating chamber 100 x 100 mm,with knob lidNano separating chamber 100 x 100 mm,with stainless steel lidNano stainless steel lid, 200 x 100 mmNano knob lid, 100 x 100 mmNano stainless steel lid, 100 x 100 mmNano filter paper for vapour conditioning the chamber,210 x 110 mm, 25 sheets
H separating chamberThe H separating chambers make optimum use of HPTLC gel layer advantages. Smallparticle size 5 µm, stringently controlled pore size and distribution, and more theoreticalbases. Excellent value for money and can be supplied for time and cost saving in 50 x 50 mm plate format or traditio-nal 100 x 100 mm format. Optimum separations are achieved even on the shortest runs.
3Sarstedt (Desaga)
Type Width Length PK Cat. No.mm mm
H separation chamberH separation chamberFrit rodsCover plateCover plate
50100050100
501005050100
11511
9.023 1509.023 1609.023 9559.023 9569.023 957
3
Syringes, disposable plastic2-piece construction: PP barrel, with PE piston. Sterile.Disposable. With Luer nozzle.4 5
Disposable syringesPolypropylene with two-piece plunger and Luer nozzle. Without needle. Supplied sterile, individually pouch-sealed inouter boxes as indicated.1
Without stopcockWithout stopcockWithout stopcockWith capillary stopcockWith capillary stopcock
11111
9.143 0559.143 0569.143 0579.143 0669.143 067
2
3
Syringes, glass, Fortuna Optimafi
Borosilicate glass. With centre glass or metal luer nozzle. Interchangeable plungers andbarrels (between identical capacity syringes). With amber graduations. Autoclavable up to +134°C.
Glass-metal syringesWith calibrated glass cylinder and plunger made of stainless steel.Plungers and cylinders interchangeable.With central metal cone.Graduated. Sterilizable ip to +200°C.
9.950 302 is a thin wall needle with a short bevel. 9.950 304 and 9.950 305 are intravenous thin wall needles.
2
Micro capillary tubesThese disposable capillary tubes fill automatically from end to end. Accuracy is better than 1 %. A capillary tube hol-der is supplied in each pack. Also contains a small bulb with aperture, which can be inserted into capillary tube andused as an effective support for filling and emptying tubes.
3
Capacity PK Cat. No.µl0.51.02.05.010.0
100100100100100
9.020 1929.020 1939.020 1949.020 1959.020 196
3
Chromatography sprayer SG 1CFC-free spraying with powerful and quiet pump.The finest spray is produced even when the battery power is low.Liquids, up to the viscosity of light oil, can be finely sprayed at the touch of a button. Particle diameter 5 to 10 µmwith a throughput of 20 ml/min. based on water. The reservoir bottle for the spray reagent is made of borosilicateglass. The bottle is screwed into the high-grade PTFE nozzle and can be changed in seconds. With quick-charging dock as a storage base. Overload protection enables continuous storage of the sprayer in thecharging station. Supplied with battery, battery charger, bottle and nozzle.
4
Type PK Cat. No.
SG 1Reservoir, 50 ml 110 9.539 0459.539 046
4
Special atomiserWith rubber blowball for nebulising reagents. Can be connected to other compressed air supplies.5
Test tube atomiserGlass atomiser for nebulising small amounts of reagents. Atomiser can be inserted in a 12 ml test tube with a groundjoint and held in position with a spring clip.1
Type PK Cat. No.
Test tube atomiser 1 9.023 990
1
Spray box with fanFor spraying TLC plates with aggressive media. Housing made of acid-resistant PVC for plates up to 200 x 200 mm.Low-noise extraction fan supplies 400 m³/h. The exhaust tube leads upwards and can be connected to NW 100 extraction tubing. Reagent droplets are collected in a separate drip tray. Size (WxDxH) 620 x 610 x 580 mm.
2
Type PK Cat. No.
Spray box with fan 1 9.020 031
2
Thermoplate SElectronically controlled, precision laboratory hotplate. For controlled, constant heating of dipped TLC plates forincreased reproducibility and evaluation. Rapid evaporation of the solvent is enabled by the direct warming of the pla-ted zone. Provides defined intermediate drying for multiple processing. Also used for reduction or evaporation of smallliquid quantities at constant temperatures or warming of reaction mixtures at a preset temperature, e.g. for colorime-tric analysis. Visual control of colour build-up. Temperature is preset and displayed digitally.
3
Hotplate: 240 x 240 mmTemperature range: 25 to 199°CAccuracy: ±2°CType PK Cat. No.
Thermoplate S 1 9.023 840
3
Analytical lamps, CabUVISWith back lighting. Constant lighting, low-pressure mercury tubes and UV-Filter for 254 and 366 nm wavelength out-put. Incident and back lighting with 8 W daylight fluorescent tube lamps and 200 x 200 mm diffusing screen. Basicunit is supplied with transparent cover screen. Optional attachment allows observation or documentation of films wit-hout a darkroom.
For UV analysis without a darkroom. High-pressure mercury lamp emits very intense radiation at 366 nm for fluoro-chemical analytical evaluation. Specially selected 254 nm filters enable optimal contrast. Minimal footprint in spite ofsimultaneous observation points for two 200 x 200 mm plates. Inclined plate angle gives comfortable viewing.Dimensions (WxDxH) 325 x 280 x 475 mm. Supply requirements 230 V.
1
Type PK Cat. No.
HP-UVIS 1 9.539 360
1
UV irradiation systems BIO-LINK- Compact and powerful, ideal for a broad range of applications- Precise measurement and control technology, non-ageing UV sensors- Choice for irradiation parameter energy or time- Easy operation: Programme memory, storage of the last parameters, programme resumes after opening of the door,auto-restart after power failure- Secure and stable construction, very easy to use- Available in 3 different wavelengths- Easy exchange of the UV tubes for wavelength change
2
Dimensions (WxDxH)Housing: 350 x 360 x 305mmInterior: 260 x 330 x 145mmType Description Tubes Wave-
lengthPK Cat. No.
nmBLX-E254BLX-E312BLX-E365
UV crosslinkerUV irradiation systemUV irradiation system5 x 8 W5 x 8 W5 x 8 W
254312365111
9.971 9239.971 9249.971 925
2
TLC Viewing cabinetsModel CN-6:- for one or two UV hand lamps model VL-6; choice of combined wavelengths 254nm, 312nm and 365nm- cabinet dimensions (WxDxH): 300 x 280 x 240mm; easy access also for large samplesModel CN-15:- integral high intensity UV lamps, extra large capacity; easy access also for large samples; white-light bulb for nor-mal observation- removable bottom panel for use with an ETX Professional Line transilluminator- cabinet dimensions (W x D x H): 505 x 415 x 280mm
33
Type Description Tubes Wave-length
PK Cat. No.nm
CN-6CN-15.LC* without UV handlampswith integrated UV tubes and white-light source -2 x 15W each -365 / 254 11 9.971 9269.971 927
Other models available on requestTLC accessories set
MACHEREY-NAGELDescription PK Cat. No.
DC simultaneous chamber for up to 5 plates, 20 x 20 cmLaboratory atomiser, glass with rubber bulbGlass capillary 1 µlOutlining templatesChromatography Paper MN 260, 7,5 x 17 cm (for saturating)
111502100
9.003 5004.004 9097.056 8494.004 9034.004 907
TLC plate cutterFor scoring and cutting glass backed TLC plates. For cost-effectiveness in plate use, or in order to give individual plates different derivatives after separation. Supplied with cutting ring and template.4
Universal application and evaluation templatesPlexiglass. Simplifies application, marking and evaluation of thin layer chromatograms. Size 200 x 200 mm.1
Type PK Cat. No.
Universal application and evaluation templates 1 9.020 131
1
Outlining templatesWith limit stops for precise positioning of the plate on the template. Provide precise pipette guidance due to triangularapertures in a 5 mm spaced grid, providing 9, 19 or 39 outlining points, depending on the size of the template. A non-slip coating means that the outlining template does not slide on the bench.
2
Width Length PK Cat. No.mm mm10050100200
10050200200
1111
9.020 1349.020 1359.020 1369.020 137
2
Laboratory dryer3 position power switch, 230V a.c. (0/700/1400W). With 2 heat/blower speed settings, nozzle attachment, cold airbutton, detachable air filter and a 3 metre mains lead.3
Type PK Cat. No.
Laboratory dryer 1 9.018 9953
Profi-labdryer PHT 303 heating levels, 3 cooling levels and 3 fan speeds giving 12 heating/ventilation combinations. Overheat protected.Removable inlet grille with filter.Supply requirement: 230 to 240V, 50 Hz, 1600 - 1800 W.