13th International Symposium on Hyphenated Techniques in Chromatography and Separation Technology (HTC-13) and 3rd International Symposium on Hyphenated Techniques for Sample Preparation (HTSP-3) January 28 - January 31, 2014 Congress Centre Oud Sint-Jan, Bruges, Belgium Symposium organized by the Royal Flemish Chemical Society (KVCV) and the Separation Science Group of the Royal Society of Chemistry (RSC) HTSP-3 Tuesday, January 28 th Short course Tuesday, January 28 th HTC-13 and HTSP-3 Wednesday, January 29 th www.comsa.be 09:00 09:10 09:20 09:30 09:40 09:50 10:00 10:10 10:20 10:30 10:40 10:50 11:00 11:10 11:20 11:30 11:40 11:50 12:00 12:10 12:20 12:30 12:40 12:50 13:00 13:10 13:20 13:30 13:40 13:50 14:00 14:10 14:20 14:30 14:40 14:50 15:00 15:10 15:20 15:30 15:40 15:50 16:00 16:10 16:20 16:30 16:40 16:50 17:00 17:10 17:20 17:30 17:40 17:50 18:00 Novel techniques and materials for sample preparation and separation (chair: Hernan Cortes) Opening HTSP-3 State-of-the-art. Alois Jungbauer (AT) Separation of large biomolecular assemblies, virusses, VLP, and protein superstructures Short course: Mass spectrometry: fundamental developments and hyphenation strategies Instructor: Prof. E. De Pauw (ULiege) In this short course, we will review the basic concepts of ionization methods from both the structural and quantitative point of view, with an emphasis on mutual requirements between the ionization, the separation methods and the mass spectrometers design. The associated activation techniques leading to fragmentation will be conceptually presented. Finally, the recently commercially available ion mobility separation and mass spectrometry imaging will be presented in terms of structural and analytical applications. Short course: continued Strategies for efficient hyphenations of (U)HPLC with MS Instructor: Dr. G. Rozing Given the strongly different environments in which (U)HPLC and Mass Spectrometry take place (liquid phase, atmospheric pressure and above versus vacuum) they are in principle alien, incompatible methods. Nevertheless early on in (U)HPLC system development, the method was successfully coupled online with MS. Since electrospray ionization has become commercially available, LC-MS evolved into a routine methodology. However, MS detection puts constraints on any (U)HPLC system, the solvents used, the solute properties and sample preparation. PLA. Janusz Pawliszyn (CA) SPME for on-site and lab quantification in complex systems - advantages and limitations KN1. Patrick Dirinck (BE) GC-MS and mass fingerprinting for understanding and measuring aroma characteristics in food products KN2. Roland Meesters (CO) Evaluation of peripheral blood microsampling techniques in combination with LC-HRMS for drug pharmacokinetics in clinical studies PLB. Valerie Pichon (FR) Biological and biomimetic approach for selective sample pretreatment KN3. Nobuo Ochiai (JP) Multi-stir bar sorptive extraction (SBSE): a new approach for SBSE using non-polar and polar coatings TU1. Tuulia Hyotylainen (FI) Sample preparation for metabolomics and for biological samples OR4. Stig Pedersen-Bjergaard (NO) Liquid-phase microextraction – Implementation in micro-chips and 96-well technology OR5. Benjamin L’homme (BE) Analysis of POPs in 20 µL blood by MEPS-GC-MS/MS KN4. Monika Wortberg (DE) Use of laboratory chromatographs as online instruments for waste-water monitoring OR1. Audrey Combes (FR) Synthesis and evaluation of a MIP for selective extraction of β-methylamino-L-alanine OR2. Beate Gruber (DE) Needle trap microextraction combined with GCxGC-TOF for the analysis of breath gas OR3. Krzysztof Gorynski (CA) Development of SPME-LC-MS method: application to pharmakinetic study Poster flash presentations Sorptive sampling techniques (Valerie Pichon) Advances in sample prep and hyphenation (Alois Jungbauer) life-science and biological samples (Janus Pawliszyn) Ambassadeur (conference venue) Novotel Coffee/Tea Exhibition/Posters Lunch Welcome reception HTSP-3 Coffee/Tea Exhibition/Posters Lunch Exhibition/Posters 09:00 09:10 09:20 09:30 09:40 09:50 10:00 10:10 10:20 10:30 10:40 10:50 11:00 11:10 11:20 11:30 11:40 11:50 12:00 12:10 12:20 12:30 12:40 12:50 13:00 13:10 13:20 13:30 13:40 13:50 14:00 14:10 14:20 14:30 14:40 14:50 15:00 15:10 15:20 15:30 15:40 15:50 16:00 16:10 16:20 16:30 16:40 16:50 17:00 17:10 17:20 17:30 17:40 17:50 18:00 Opening session HTC (chairs: Hernan Cortes, Pat Sandra, Freddy Adams) Opening HTC-13 TU2. Koen Sandra (BE) Biopharmaceutical analysis TU3. Didier Thiebaut (FR) The role of hyphenated techniques in the petroleum industry TU4. Ian Wilson (UK) Hyphenation for metabonomics/metabolomics OR6. Gauthier Eppe (BE) Innovative strategies by ion-mobility MS OR7. Emmanuelle Bichon (FR) Hard competition between GC and LC to pro-vide reliable signals for metabolites by MS OR8. Sebastien Carpentier (BE) Label free quantitative proteomics: data independent or data dependent analysis? OR9. Ann van Schepdael (BE) CE/MS for the study of matrix metalloproteinase OR13. Eleanor Riches (GB) Evaluating the potential of ion mobility MS for crude oil analysis OR14. Julie Herniman (GB) UPC2-MS applied to the analysis of renewable biofuels OR15. Sam Whitmarsh (GB) Forensic fingerprinting in the petrochemical industry using hyphenated techniques OR22. Jose Di Mavungu (BE) Comparative metabolomics based on UHPLC/Orbitrap and microLC/triple TOF HRMS OR21. Thorsten Teutenberg (DE) Comprehensive nano/cap-LC´LC-MS for the screening analysis of complex samples Life-time-achievement award PL1. Gert Desmet (BE) Instrument influences on observed column performance PL2. Hans-Gerd Janssen (NL) From good multi-dimensional data to great multi-dimensional information KN5. Andre De Villiers (SA) Systematic optimisation and evaluation of LC×LC separation of phenolics: Fundamental aspects and selected applications KN6. Luigi Mondello (IT) Rapid collection of high solute amounts by using an on-line four-dimensional LC-GC-GC-GC preparative instrument KN7. Robert Shellie (AU) You are what you eat: Using GCxGC to unravel the diet of the whale shark Rhincodon typus KN8. Tadeusz Gorecki (CA) Towards a simpler future in comprehensive two dimensional gas chromatography KN9. Frank David (BE) New hyphenated techniques for aroma analysis KN10. Aviv Amirav (IL) Pulsed flow modulation with cold EI - The emergence of GC↔GC-MS OR10. Szabolcs Fekete (CH) Possibilities and limitations of current state-of-the-art column technology OR17. Sara Ongay (NL) Free and total desmosine and isodesmosine in human urine by LC-MS OR18. Karsten Hiller (LU) Stable-isotope labeling to profile dynamics of cellular metabolism OR19. John Barr (USA) Development of an alternative potency assay for influenza vaccines using SEC-online trypsin digestion-LC-MS/MS OR20. Mike De Vrieze (BE) Modeling central nervous system drug activity OR11. Jirka Urban (CZ) Hypercrosslinking modification of organic polymer-based monoliths OR12. Fabien Brothier (FR) Miniaturization of selective sorbents: antibody/aptamer-modified monoliths KN11. Michael Laemmerhofer (DE) Tailoring chromatographic selectivity KN12. Deirdre Cabooter (BE) Detailed characterization of the kinetic performance of first and second generation silica monolithic columns KN13. Hian-Kee Lee (SG) Translational environmental analytical chemistry: laboratory-to-field possibilities KN14. Emily Hilder (AU) Polymeric monolithic materials for miniaturised bioanalysis OR16. Esme Candish (AU) Rapid sample preparation using minaturized solid phase extraction Poster flash presentations Poster flash presentations Poster flash presentations Poster flash presentations Mass Spectrometry (Bart Devreese) LCn and GCn (Aviv Amirav) (Bio)pharma (Fred Regnier) GC-MS and GC↔GC (Pat Sandra) Column technology for sample preparation and HPLC (Gert Desmet) RSC: (Petro-)Chemical industry (John Langely) Sample preparation (Tuulia Hyotylainen) LC-MS and biomarkers (Davy Guillarme) Hyphenated strategies (Ralf Zimmermann) Ambassadeur Erasmus Morus Reception Town Hall (19:00) / Processing and analysis of Belgian Beer (20:30 tickets required) Coffee/Tea Exhibition/Posters Coffee/Tea Exhibition/Posters Lunch Exhibition/Posters Product seminars (13:10 - 13:50) Product seminar Waters Product seminar FMS/Campro Scientific Product seminar Gerstel Product seminar Peak Scientific
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13th International Symposium on Hyphenated Techniques in
Chromatography and Separation Technology (HTC-13)
and
3rd International Symposium on Hyphenated Techniques
for Sample Preparation (HTSP-3)
January 28 - January 31, 2014
Congress Centre Oud Sint-Jan,Bruges, Belgium
Symposium organized by the Royal Flemish Chemical Society (KVCV)
and theSeparation Science Group of the Royal Society of Chemistry (RSC)
HTSP-3 Tuesday, January 28th Short course Tuesday, January 28th HTC-13 and HTSP-3 Wednesday, January 29th
Separation of large biomolecular assemblies, virusses, VLP, and protein superstructures
Short course:
Mass spectrometry: fundamental developments and hyphenation strategies
Instructor:Prof. E. De Pauw (ULiege)
In this short course, we will review the basic concepts of ionization methods from both the structural and quantitative point of view, with an emphasis on mutual requirements between the ionization, the separation methods and the mass spectrometers design. The associated activation techniques leading to fragmentation
will be conceptually presented. Finally, the recently commercially available ion mobility separation and mass spectrometry imaging will be presented in terms of structural and analytical applications.
Short course: continued
Strategies for efficient hyphenations of (U)HPLC with MS
Instructor:Dr. G. Rozing
Given the strongly different environments in which (U)HPLC and Mass Spectrometry take place (liquid phase, atmospheric pressure and above versus vacuum) they are in principle alien, incompatible methods.
Nevertheless early on in (U)HPLC system development, the method was successfully coupled online with MS. Since electrospray ionization has become commercially available, LC-MS evolved into a routine methodology.
However, MS detection puts constraints on any (U)HPLC system, the solvents used, the solute properties and sample preparation.
PLA. Janusz Pawliszyn (CA)SPME for on-site and lab quantification in complex
systems - advantages and limitations
KN1. Patrick Dirinck (BE)GC-MS and mass fingerprinting for understanding
and measuring aroma characteristics in food products
KN2. Roland Meesters (CO)Evaluation of peripheral blood microsampling techniques in combination with LC-HRMS for drug pharmacokinetics
in clinical studies
PLB. Valerie Pichon (FR)Biological and biomimetic approach for selective
sample pretreatment
KN3. Nobuo Ochiai (JP)Multi-stir bar sorptive extraction (SBSE): a new approach for
SBSE using non-polar and polar coatings
TU1. Tuulia Hyotylainen (FI)Sample preparation for metabolomics and
for biological samples
OR4. Stig Pedersen-Bjergaard (NO)Liquid-phase microextraction – Implementation in micro-chips
and 96-well technology
OR5. Benjamin L’homme (BE)Analysis of POPs in 20 µL blood by MEPS-GC-MS/MS
KN4. Monika Wortberg (DE)Use of laboratory chromatographs as online instruments for
waste-water monitoring
OR1. Audrey Combes (FR)Synthesis and evaluation of a MIP for selective extraction
of β-methylamino-L-alanineOR2. Beate Gruber (DE)
Needle trap microextraction combined with GCxGC-TOF for the analysis of breath gas
OR3. Krzysztof Gorynski (CA)Development of SPME-LC-MS method: application