13 dioxin detection 戴戴戴 : 戴戴戴戴 戴戴戴戴戴戴戴戴戴 戴戴戴戴戴戴戴戴
Jan 30, 2016
13 dioxin detection:
(dioxin): 210 75 polychlorinated dibenzo--dioxinsPCDDs135 polychlorinated dibenzofuransPCDFs 210172,3,7,8-(2,3,7,8-TCDD)
209(PCB)12, (dioxin-like congener PCB)
2,3,7,8-(2,3,7,8-TCDD)
toxic equivalent quantitiesTEQ
2,3,7,8-TCDD toxic equivalent quantities
toxic equivalent factorTEFPCDDs 2,3,7,8-TCDD PCDDs PCDFs TEF TEQTEQ
Toxic Equivalent Factors
/HRGC/HRMS2~3
Chemically Activated Luciferase Expression CALUX
CALUX
H4IIE rat-hepatomaH4IIEluciferase geneXRE
CALUX 3~4
Luciferase is a generic term for the class of oxidative enzymes used in bioluminescence.One famous example is the firefly luciferase.In luminescent reactions, light is produced by the oxidation of a luciferin
luciferin + O2 oxyluciferin + light
luciferin + ATP luciferyl adenylate + PPi luciferyl adenylate + O2 oxyluciferin + AMP + light
Enzyme-linked immunosorbentassayELISA Electrochemical biosensor Surface plasmon resonance immunosensor Piezoelectric quartz crystal microbalancebiosensor2,3,7,8-TCDD AhR-ARNT
dioxin binding peptidepeptide library Combinatorial Chemistry
Combinatorial ChemistryMerrifield: the solid-phase synthesis of peptides.
Furka, 1988 : divide-couple-recombine, mixing-divide-coupleHoughten, 1991 : mesh packets (Nature), positional scanning